2022
Arginase-1 Is Required for Macrophage-Mediated Renal Tubule Regeneration
Shin NS, Marlier A, Xu L, Doilicho N, Linberg D, Guo J, Cantley LG. Arginase-1 Is Required for Macrophage-Mediated Renal Tubule Regeneration. Journal Of The American Society Of Nephrology 2022, 33: 1077-1086. PMID: 35577558, PMCID: PMC9161787, DOI: 10.1681/asn.2021121548.Peer-Reviewed Original ResearchConceptsIschemia-reperfusion injuryTubular cell proliferationArginase-1Contralateral nephrectomyRenal repairFl/Littermate controlsTubular cellsReceptor 1GM-CSFRenal tubular cell proliferationRenal tubule regenerationMacrophage scavenger receptor 1Mannose receptor 1Cell proliferative responsesCell proliferationScavenger receptor 1Coculture of macrophagesDead cell debrisKidney injuryKidney repairRenal responseProinflammatory activationTubule regenerationMouse survival
2019
Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury
Xu L, Sharkey D, Cantley LG. Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury. Journal Of The American Society Of Nephrology 2019, 30: 1825-1840. PMID: 31315923, PMCID: PMC6779361, DOI: 10.1681/asn.2019010068.Peer-Reviewed Original ResearchConceptsIschemia/reperfusion injuryWild-type miceTubular cellsTubular injuryReperfusion injuryImmune cellsKidney ischemia/reperfusion injuryUnilateral ischemia/reperfusion injuryMCP-1/CCR2Monocyte chemoattractant protein-1Initial kidney damageInjured tubular cellsKidney 14 daysKidney injury markersProgressive interstitial fibrosisProfibrotic growth factorsChemoattractant protein-1MCP-1 receptorGranulocyte-macrophage colony-stimulating factorRenal tubular cellsNumber of macrophagesTime of repairColony-stimulating factorCoculture of macrophagesMacrophages persist
2014
Click Hybridization of Immune Cells and Polyamidoamine Dendrimers
Xu L, Zolotarskaya OY, Yeudall WA, Yang H. Click Hybridization of Immune Cells and Polyamidoamine Dendrimers. Advanced Healthcare Materials 2014, 3: 1430-1438. PMID: 24574321, PMCID: PMC4133313, DOI: 10.1002/adhm.201300515.Peer-Reviewed Original Research
2012
25-Hydroxycholesterol-3-sulfate attenuates inflammatory response via PPARγ signaling in human THP-1 macrophages
Xu L, Shen S, Ma Y, Kim JK, Rodriguez-Agudo D, Heuman DM, Hylemon PB, Pandak WM, Ren S. 25-Hydroxycholesterol-3-sulfate attenuates inflammatory response via PPARγ signaling in human THP-1 macrophages. AJP Endocrinology And Metabolism 2012, 302: e788-e799. PMID: 22275753, PMCID: PMC3330710, DOI: 10.1152/ajpendo.00337.2011.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Inflammatory AgentsBlotting, WesternCell NucleusCholesterol EstersCytokinesCytosolEnzyme-Linked Immunosorbent AssayHumansHydroxycholesterolsHypoglycemic AgentsI-kappa B ProteinsLipopolysaccharidesMacrophagesNF-kappa BPPAR gammaProtein TransportReal-Time Polymerase Chain ReactionRNA, MessengerRNA, Small InterferingRosiglitazoneSignal TransductionThiazolidinedionesConceptsNF-κB protein levelsPeroxisome proliferator-activated receptorInflammatory responseNuclear PPARγReporter gene assayLipid metabolismNuclear receptor peroxisome proliferator-activated receptorIL-1β expressionLiver X receptor ligandsProtein levelsActivation of PPARγHuman THP-1 macrophagesProliferator-activated receptorGene assayLuciferase activityTHP-1 macrophagesPPARγ-specific antagonistIκB/NF-κBX receptor ligandsIL-1βPromoter reporter gene assaysCholesterol metabolitesPPARγ activityPotent regulatory moleculesNF-κB
2008
25-Hydroxycholesterol-3-sulfate regulates macrophage lipid metabolism via the LXR/SREBP-1 signaling pathway
Ma Y, Xu L, Rodriguez-Agudo D, Li X, Heuman DM, Hylemon PB, Pandak WM, Ren S. 25-Hydroxycholesterol-3-sulfate regulates macrophage lipid metabolism via the LXR/SREBP-1 signaling pathway. AJP Endocrinology And Metabolism 2008, 295: e1369-e1379. PMID: 18854425, PMCID: PMC2603552, DOI: 10.1152/ajpendo.90555.2008.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCell ProliferationCells, CulturedCholesterol EstersDNA-Binding ProteinsDose-Response Relationship, DrugGene Expression RegulationHumansHydroxycholesterolsLipid MetabolismLipidsLiver X ReceptorsMacrophagesModels, BiologicalOrphan Nuclear ReceptorsReceptors, Cytoplasmic and NuclearSignal TransductionSterol Regulatory Element Binding Protein 1SulfatesConceptsLXR/SREBPFatty acid synthaseSREBP-1Lipid metabolismFatty acid synthetic pathwayKey transcriptional regulatorMacrophage lipid metabolismImportant regulatory moleculesTHP-1-derived macrophagesMature proteinTranscriptional regulatorsAcetyl-CoA carboxylase 1MRNA levelsFAS mRNA levelsKey proteinsGene expressionRegulatory moleculesHuman THP-1-derived macrophagesAcid synthaseSREBPLipid synthesisOxysterol sulfationLXR ligandsCarboxylase 1Cell proliferation