2022
Donor extracellular vesicle trafficking via the pleural space represents a novel pathway for allorecognition after lung transplantation
Habertheuer A, Chatterjee S, Sada Japp A, Ram C, Korutla L, Ochiya T, Li W, Terada Y, Takahashi T, Nava RG, Puri V, Kreisel D, Vallabhajosyula P. Donor extracellular vesicle trafficking via the pleural space represents a novel pathway for allorecognition after lung transplantation. American Journal Of Transplantation 2022, 22: 1909-1918. PMID: 35285127, DOI: 10.1111/ajt.17023.Peer-Reviewed Original ResearchConceptsMediastinal lymph nodesLymph nodesLung transplantationDonor antigensPleural spaceBronchial anastomosisGraft-draining lymph nodesLocoregional lymph nodesLung transplant recipientsPeripheral lymph nodesTransplant recipientsAllorecognition pathwaysPulmonary transplantationMHC-IIPleural fluidRat modelT cellsLymphatic drainageTransplantationDonor extracellular vesiclesPleural lymphaticsCell traffickingRapid rejectionAlternative pathwayAntigen
2021
Circulating Donor Lung-specific Exosome Profiles Enable Noninvasive Monitoring of Acute Rejection in a Rodent Orthotopic Lung Transplantation Model
Habertheuer A, Ram C, Schmierer M, Chatterjee S, Hu R, Freas A, Zielinski P, Rogers W, Silvestro EM, McGrane M, Moore JS, Korutla L, Siddiqui S, Xin Y, Rizi R, Tao J, Kreisel D, Naji A, Ochiya T, Vallabhajosyula P. Circulating Donor Lung-specific Exosome Profiles Enable Noninvasive Monitoring of Acute Rejection in a Rodent Orthotopic Lung Transplantation Model. Transplantation 2021, 106: 754-766. PMID: 33993180, DOI: 10.1097/tp.0000000000003820.Peer-Reviewed Original ResearchConceptsAcute rejectionDonor exosomesExosome profileSyngeneic controlsRat orthotopic lung transplant modelChronic lung allograft dysfunctionOrthotopic lung transplantation modelLung transplant modelPosttransplant time pointsAcute rejection episodesLung allograft dysfunctionLung transplant patientsLung transplant rejectionLung transplantation modelEarly therapeutic interventionMajor risk factorTime-sensitive diagnosisDevelopment of biomarkersAllograft dysfunctionRejection episodesLewis recipientsTransplant patientsPulmonary allograftsRecipient bloodAllogeneic grafts
2020
Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes
Yu M, Agarwal D, Korutla L, May CL, Wang W, Griffith NN, Hering BJ, Kaestner KH, Velazquez OC, Markmann JF, Vallabhajosyula P, Liu C, Naji A. Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes. Nature Metabolism 2020, 2: 1013-1020. PMID: 32895576, PMCID: PMC7572844, DOI: 10.1038/s42255-020-0269-7.Peer-Reviewed Original ResearchConceptsIslet transplantationSubcutaneous spacePancreatic isletsNon-human primate modelSyngeneic islet transplantationType 1 diabetesTreatment of typeLack of neovascularizationPancreatic islet transplantationNew therapeutic paradigmHuman pancreatic isletsSustained normoglycaemiaGraft survivalHypoxic cell deathPrimate modelPreclinical modelsAnimal modelsTransplantationSubcutaneous transplantationTherapeutic paradigmDiabetesEuglycaemiaCell deathIsletsTransplantation methodologiesSyncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia
Levine L, Habertheuer A, Ram C, Korutla L, Schwartz N, Hu RW, Reddy S, Freas A, Zielinski PD, Harmon J, Molugu SK, Parry S, Vallabhajosyula P. Syncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia. Scientific Reports 2020, 10: 6398. PMID: 32286341, PMCID: PMC7156695, DOI: 10.1038/s41598-020-62193-7.Peer-Reviewed Original ResearchConceptsSyncytin-1Preeclampsia groupMaternal circulationExtracellular vesiclesCommon placental pathologyHigh expressionDiagnosis of preeclampsiaConditions of preeclampsiaBone alkaline phosphatasePotential physiologic roleTissue-specific biomarkersExtracellular microvesiclesPlasma extracellular vesiclesAlkaline phosphatasePreeclampsia subjectsNormal pregnancyPlacental pathologyCell extracellular vesiclesImproved biomarkersMaternal bloodPreeclampsiaPlacental syncytiotrophoblastMaternal plasmaPlacental alkaline phosphatasePhysiologic role
2019
Circulating exosomes derived from transplanted progenitor cells aid the functional recovery of ischemic myocardium
Saha P, Sharma S, Korutla L, Datla SR, Shoja-Taheri F, Mishra R, Bigham GE, Sarkar M, Morales D, Bittle G, Gunasekaran M, Ambastha C, Arfat MY, Li D, Habertheuer A, Hu R, Platt MO, Yang P, Davis ME, Vallabhajosyula P, Kaushal S. Circulating exosomes derived from transplanted progenitor cells aid the functional recovery of ischemic myocardium. Science Translational Medicine 2019, 11 PMID: 31118291, PMCID: PMC6857931, DOI: 10.1126/scitranslmed.aau1168.Peer-Reviewed Original ResearchConceptsCardiac progenitor cellsMessenger RNACondition-specific mannerComputational pathway analysisProgenitor cellsCellular messenger RNAsCardiosphere-derived cellsProgenitor cell typesStem cell fieldCell-derived exosomesPathway analysisMicroRNA (miRNA) cargoCell typesExosomesTransplanted progenitor cellsCPC exosomesConditional activityPlasma exosomesC-kitCellsCell fieldTransplanted cardiac progenitor cellsRNAMyocardial infarction modelPathwayNoninvasive diagnosis of recurrent autoimmune type 1 diabetes after islet cell transplantation
Korutla L, Rickels MR, Hu RW, Freas A, Reddy S, Habertheuer A, Harmon J, Korutla V, Ram C, Naji A, Vallabhajosyula P. Noninvasive diagnosis of recurrent autoimmune type 1 diabetes after islet cell transplantation. American Journal Of Transplantation 2019, 19: 1852-1858. PMID: 30801971, PMCID: PMC7043773, DOI: 10.1111/ajt.15322.Peer-Reviewed Original ResearchConceptsIslet cell transplantationAutoimmune type 1 diabetesType 1 diabetesCell transplantationT1D autoimmunityNoninvasive diagnosisPancreatic β-cell injuryGlutamic acid decarboxylase 65Time-matched analysisΒ-cell injuryTransplanted β-cellsHyperglycemia onsetIslet injuryRecurrent autoimmunityHypoglycemic unawarenessTime-specific increaseIslet autoantigensCurative therapyT1D patientsImmunologic rejectionExogenous insulinInjury typeControl subjectsIslet transplantationDonor islets
2017
Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue
Vallabhajosyula P, Korutla L, Habertheuer A, Yu M, Rostami S, Yuan CX, Reddy S, Liu C, Korutla V, Koeberlein B, Trofe-Clark J, Rickels MR, Naji A. Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue. Journal Of Clinical Investigation 2017, 127: 1375-1391. PMID: 28319051, PMCID: PMC5373894, DOI: 10.1172/jci87993.Peer-Reviewed Original ResearchConceptsRenal transplantationImmunologic rejectionTransplanted tissueClinical settingBiomarker platformAppearance of hyperglycemiaIslet transplant modelIslet β-cellsDonor exosomesTransplant isletsHLA antibodiesRecipient bloodTransplant modelRecipient circulationXenogeneic modelRecipient plasmaRenal epithelial cellsTransplant tissueHuman isletsΒ-cellsBiomarker potentialTransplantationNoninvasive windowEpithelial cellsExosomal microRNAsEx Vivo Lung Perfusion Model to Study Pulmonary Tissue Extracellular Microvesicle Profiles
Vallabhajosyula P, Korutla L, Habertheuer A, Reddy S, Schaufler C, Lasky J, Diamond J, Cantu E. Ex Vivo Lung Perfusion Model to Study Pulmonary Tissue Extracellular Microvesicle Profiles. The Annals Of Thoracic Surgery 2017, 103: 1758-1766. PMID: 28242077, DOI: 10.1016/j.athoracsur.2016.11.074.Peer-Reviewed Original ResearchConceptsEVLP systemWestern blot analysisTransplant groupExtracellular microvesiclesVivo lung perfusion modelTime pointsMarginal donor lungsLung perfusion modelFollowing time pointsTranscription-polymerase chain reactionBlot analysisEV size distributionMarginal lungsNontransplant groupDonor lungsLung recoveryLung perfusionSteen solutionPolymerase chain reactionPulmonary parenchymaEV protein cargoPathologic processesPerfusion modelTissue-specific markersPerfusate exchange
2013
NAC1, A POZ/BTB protein interacts with Parkin and may contribute to Parkinson’s disease
Korutla L, Furlong H, Mackler S. NAC1, A POZ/BTB protein interacts with Parkin and may contribute to Parkinson’s disease. Neuroscience 2013, 257: 86-95. PMID: 24231739, DOI: 10.1016/j.neuroscience.2013.11.001.Peer-Reviewed Original ResearchMeSH KeywordsAged, 80 and overAnimalsBrainCell Line, TransformedCentral Nervous SystemCysteine Proteinase InhibitorsCytoplasmDown-RegulationGlutathione TransferaseHumansImmunoprecipitationLeupeptinsMaleMiceNerve Tissue ProteinsNeuronsParkinson DiseaseProteasome Endopeptidase ComplexRepressor ProteinsUbiquitin-Protein LigasesConceptsNucleus accumbens-1Neuronal cell deathParkinson's diseaseDisease patientsParkinson's disease patientsProteasomal activityPOZ/BTB proteinCell deathNeuronal cellsDiseaseParkin levelsProtein levelsCell susceptibilityParkin proteinProteasome protein levelsPatientsProteasome activityUbiquitin-dependent proteasome degradationCell viabilityParkin degradationDeathParkinKey proteinsProteasome degradationToxicity
2008
NAC1, a POZ/BTB protein that functions as a corepressor
Korutla L, Wang P, Jackson T, Mackler S. NAC1, a POZ/BTB protein that functions as a corepressor. Neurochemistry International 2008, 54: 245-252. PMID: 19121354, DOI: 10.1016/j.neuint.2008.12.008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCentral Nervous SystemGenes, ReporterHumansKruppel-Like Transcription FactorsMiceNeoplasm ProteinsNerve Tissue ProteinsPromyelocytic Leukemia Zinc Finger ProteinProtein IsoformsProtein Structure, TertiaryProto-Oncogene ProteinsRecombinant Fusion ProteinsRepressor ProteinsTranscription, GeneticConceptsPOZ/BTB proteinBTB proteinsPOZ/BTB domainFusion proteinVP16 activation domainGST pulldown assaysGAL4 fusion proteinsTranscriptional repressor proteinZinc finger proteinProtein-protein interactionsB fusion proteinFinger proteinActivation domainTransient assaysNAC1 functionPulldown assaysRepressor proteinBTB domainNon-neuronal cellsTranscriptional inhibitionCorepressorNeuronal cellsProteinMature CNSSelective interaction
2007
NAC1, a cocaine‐regulated POZ/BTB protein interacts with CoREST
Korutla L, Degnan R, Wang P, Mackler S. NAC1, a cocaine‐regulated POZ/BTB protein interacts with CoREST. Journal Of Neurochemistry 2007, 101: 611-618. PMID: 17254023, DOI: 10.1111/j.1471-4159.2006.04387.x.Peer-Reviewed Original ResearchConceptsPOZ/BTB proteinPOZ/BTB domainBTB proteinsBTB domainProtein-protein interactionsHEK293T cellsNeuro-2a cellsT cellsTranscriptional regulatorsRepressor proteinRat brain samplesBrain samplesCoRESTCoimmunoprecipitation studiesHomodimer formationHomodimer assemblyRepressor mechanismProtein expressionProteinDirect interactionEndogenous interactionPresent studyPox virus
2005
The POZ/BTB protein NAC1 interacts with two different histone deacetylases in neuronal‐like cultures
Korutla L, Wang P, Mackler S. The POZ/BTB protein NAC1 interacts with two different histone deacetylases in neuronal‐like cultures. Journal Of Neurochemistry 2005, 94: 786-793. PMID: 16033423, DOI: 10.1111/j.1471-4159.2005.03206.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBlotting, WesternButyratesCells, CulturedEnzyme InhibitorsGene Expression RegulationHippocampusHistone DeacetylasesHumansHydroxamic AcidsImmunoprecipitationMiceNerve Tissue ProteinsNeuroblastomaNeuronsPlasmidsProtein BindingProtein IsoformsRatsRepressor ProteinsTranscription, GeneticTransfectionTwo-Hybrid System TechniquesConceptsPOZ/BTB proteinProtein-protein interactionsBTB proteinsHistone deacetylasesPOZ domainTwo-hybridGlutathione S-transferase pulldownRecruit histone deacetylasesDifferent histone deacetylasesTranscriptional repressionGST pulldownSNAC1Histone deacetylase inhibitionCorepressorDeacetylasesRepressionDeacetylase inhibitionProteinPulldownHDAC-3NaC1MSin3ANucleus accumbensCNS regionsCoimmunoprecipitation
2003
The mouse nac1 gene, encoding a cocaine-regulated bric-a-brac tramtrac broad complex/pox virus and zinc finger protein, is regulated by ap1
Mackler S, Homan Y, Korutla L, Conti A, Blendy J. The mouse nac1 gene, encoding a cocaine-regulated bric-a-brac tramtrac broad complex/pox virus and zinc finger protein, is regulated by ap1. Neuroscience 2003, 121: 355-361. PMID: 14521994, DOI: 10.1016/s0306-4522(03)00376-2.Peer-Reviewed Original ResearchAnimalsBlotting, NorthernBlotting, SouthernCell DifferentiationCell Line, TumorChromosomes, Human, Pair 8Cloning, MolecularColforsinDNA ProbesDNA-Binding ProteinsDrosophilaDrosophila ProteinsExonsGene ExpressionHumansMiceMice, Inbred C57BLMutagenesisNerve Tissue ProteinsNeuroblastomaPromoter Regions, GeneticRatsRepressor ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSequence AlignmentSequence AnalysisTranscription Factor AP-1Transcription FactorsTransfectionZinc Fingers
1996
Inhibition of Vesicular Stomatitis Virus Replication by Epidermal Growth Factor in Human Epidermoid A-431 Cells
Korutla L, Kumar R. Inhibition of Vesicular Stomatitis Virus Replication by Epidermal Growth Factor in Human Epidermoid A-431 Cells. Biochemical And Biophysical Research Communications 1996, 220: 670-674. PMID: 8607823, DOI: 10.1006/bbrc.1996.0462.Peer-Reviewed Original ResearchConceptsEpidermal growth factorVesicular stomatitis virusInfectious viral particle productionAnti-EGF receptor monoclonal antibodyGrowth factorObserved antiviral effectReceptor monoclonal antibodyVesicular stomatitis virus replicationHuman epidermoid AVSV protein synthesisAntiviral effectViral particle productionAntiviral actionVSV infectionPrior treatmentViral protein synthesisVSV replicationPostinfection treatmentVirus replicationMonoclonal antibodiesInhibitory effectEarly effectsProtein synthesisSignificant inhibitionCell protectionMechanism of interferon action: in vivo activation of 91 kDa transcription factor.
Korutla L, Kumar R. Mechanism of interferon action: in vivo activation of 91 kDa transcription factor. Anticancer Research 1996, 16: 2789-95. PMID: 8917387.Peer-Reviewed Original ResearchConceptsLive human cellsHuman cellsSpecific cellular genesProtein tyrosine kinasesRegulation of expressionTreatment of cellsProtein phosphotyrosineCellular genesEarly biochemical eventsDistinct biological effectsTranscription factorsTyrosine phosphorylationTyrosine kinaseSodium orthovanadateBiochemical eventsCell growthInterferon actionBiological effectsPossible involvementCellsActivationVivo activationKinaseGenesPhosphorylation
1995
Induction of Expression of Interferon-Stimulated Gene Factor-3 (ISGF-3) Proteins by Interferons
Kumar R, Korutla L. Induction of Expression of Interferon-Stimulated Gene Factor-3 (ISGF-3) Proteins by Interferons. Experimental Cell Research 1995, 216: 143-148. PMID: 7529187, DOI: 10.1006/excr.1995.1018.Peer-Reviewed Original ResearchCell NucleusCytosolDNA-Binding ProteinsGene Expression RegulationHumansInterferon Type IInterferon-gammaInterferon-Stimulated Gene Factor 3Interferon-Stimulated Gene Factor 3, gamma SubunitKineticsPhosphorylationPhosphotyrosineRecombinant ProteinsRNA, MessengerTranscription FactorsTumor Cells, CulturedTyrosineGrowth inhibition of human acute promyelocytic leukemia NB-4 cells by interferons and all-trans retinoic acid: trans-modulation of inducible gene expression pathways.
Kumar R, Korutla L. Growth inhibition of human acute promyelocytic leukemia NB-4 cells by interferons and all-trans retinoic acid: trans-modulation of inducible gene expression pathways. Anticancer Research 1995, 15: 353-60. PMID: 7763006.Peer-Reviewed Original Research
1994
Inhibitory effect of curcumin on epidermal growth factor receptor kinase activity in A431 cells
Korutla L, Kumar R. Inhibitory effect of curcumin on epidermal growth factor receptor kinase activity in A431 cells. Biochimica Et Biophysica Acta 1994, 1224: 597-600. PMID: 7803521, DOI: 10.1016/0167-4889(94)90299-2.Peer-Reviewed Original ResearchConceptsReceptor's intrinsic kinase activityShort-term treatmentObserved early effectsTime-dependent mannerA431 cellsReceptor kinase activityKinase activityInhibitory effectEarly effectsAntigrowth actionEGF-induced tyrosine phosphorylationEpidermoid carcinoma A431 cellsHuman epidermoid carcinoma A431 cellsIntrinsic kinase activityEpidermal growth factor receptor kinase activityEGF receptorCurcuminCell growthCellsTyrosine phosphorylationDoseCell cycle-dependent modulation of alpha-interferon-inducible gene expression and activation of signaling components in Daudi cells.
Kumar R, Korutla L, Zhang K. Cell cycle-dependent modulation of alpha-interferon-inducible gene expression and activation of signaling components in Daudi cells. Journal Of Biological Chemistry 1994, 269: 25437-25441. PMID: 7929242, DOI: 10.1016/s0021-9258(18)47269-9.Peer-Reviewed Original ResearchMeSH Keywords2',5'-Oligoadenylate SynthetaseBase SequenceBurkitt LymphomaCell CycleDNA-Binding ProteinsEnzyme ActivationG1 PhaseGene Expression RegulationHumansInterferon-alphaJanus Kinase 1Molecular Sequence DataProtein-Tyrosine KinasesProteinsS PhaseSignal TransductionSTAT1 Transcription FactorTrans-ActivatorsTumor Cells, CulturedTYK2 KinaseConceptsTyk-2 kinaseJAK-1Synthetase proteinTyrosine phosphorylationGene expressionCell cycle-dependent modulationCell cycleCell cycle-dependent expressionCell cycle-dependent changesInducible gene expressionGene factor 3Unique expression patternCycle-dependent expressionG1/S phaseCell cycle progressionG1/SIFN-inducible gene expressionG2/M.Human Burkitt lymphoma Daudi cellsInduction of expressionG1/S blockG2/MDaudi cellsIFN-alpha-inducible genesCycle-dependent changes