2016
CJD and Scrapie Require Agent‐Associated Nucleic Acids for Infection
Botsios S, Manuelidis L. CJD and Scrapie Require Agent‐Associated Nucleic Acids for Infection. Journal Of Cellular Biochemistry 2016, 117: 1947-1958. PMID: 26773845, DOI: 10.1002/jcb.25495.Peer-Reviewed Original ResearchConceptsTSE agentsTransmissible spongiform encephalopathiesAdaptive immune responsesDegenerative brain changesInfectious particlesHost prion proteinGT1 neuronal cellsForms of PrPLymphoreticular tissuesBrain changesImmune responseTSE strainsNeuronal cellsNeurodegenerative diseasesLatent virusInfectivity assaysSpongiform encephalopathiesNucleic acid genomeTitersEpidemic spreadViral structuresPrion proteinHost proteinsVirusHost components
2015
Rapid chemical decontamination of infectious CJD and scrapie particles parallels treatments known to disrupt microbes and biofilms
Botsios S, Tittman S, Manuelidis L. Rapid chemical decontamination of infectious CJD and scrapie particles parallels treatments known to disrupt microbes and biofilms. Virulence 2015, 6: 787-801. PMID: 26556670, PMCID: PMC4826107, DOI: 10.1080/21505594.2015.1098804.Peer-Reviewed Original ResearchConceptsInfectious titerInnate immune responseHuman CJDResistant virusesIatrogenic infectionGT1 cellsBrain changesImmune responseInfectious agentsTSE agentsScrapie agentCJDPrion protein amyloidSheep scrapieVirulent microbesInfectious particlesMin exposureIntrinsic resistanceTreatmentTitersPrP amyloidScrapieAmyloidCultured cellsDeep proteomic analysisProteomic analysis of host brain components that bind to infectious particles in Creutzfeldt‐Jakob disease
Kipkorir T, Colangelo CM, Manuelidis L. Proteomic analysis of host brain components that bind to infectious particles in Creutzfeldt‐Jakob disease. Proteomics 2015, 15: 2983-2998. PMID: 25930988, PMCID: PMC4601564, DOI: 10.1002/pmic.201500059.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCase-Control StudiesCreutzfeldt-Jakob SyndromeCyclin-Dependent Kinase Inhibitor p18CyclophilinsHSC70 Heat-Shock ProteinsHumansMicePrionsProteinsProteomicsConceptsCreutzfeldt-Jakob diseaseInfectious agentsTransmissible encephalopathiesNew therapeutic initiativesBrain particlesCausal infectious agentInfectious particlesHost prion proteinHost immune recognitionSynapsin-2Such therapyHost proteinsTherapeutic initiativesImmune recognitionStrain-specific patternsCommon pathwayCross-species transmissionHigh infectivityDiseaseViral pathwaysProteomic analysisHost targetsViral proteinsViral processingBrain components
2014
Highly Infectious CJD Particles Lack Prion Protein but Contain Many Viral‐Linked Peptides by LC‐MS/MS
Kipkorir T, Tittman S, Botsios S, Manuelidis L. Highly Infectious CJD Particles Lack Prion Protein but Contain Many Viral‐Linked Peptides by LC‐MS/MS. Journal Of Cellular Biochemistry 2014, 115: 2012-2021. PMID: 24933657, PMCID: PMC7166504, DOI: 10.1002/jcb.24873.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainChromatography, LiquidCreutzfeldt-Jakob SyndromeHumansHuntingtin ProteinMiceNerve Tissue ProteinsPeptidesProteomicsPrPSc ProteinsTandem Mass SpectrometryVirulenceConceptsSporadic CJDMouse brainTransmissible encephalopathiesNormal human brain samplesHost prion proteinHuman brain samplesSCJD brainsPrion proteinAmyloid pathologyAPP processingNew therapiesUninfected controlsBrain homogenatesBrain samplesCellular findingsDetectable PrPNeurodegenerative diseasesSheep scrapieInfectious titerBrainProteinase KCJDLC-MS/MSViral motifsInfectious form
2012
Continuous Production of Prions after Infectious Particles Are Eliminated: Implications for Alzheimer’s Disease
Miyazawa K, Kipkorir T, Tittman S, Manuelidis L. Continuous Production of Prions after Infectious Particles Are Eliminated: Implications for Alzheimer’s Disease. PLOS ONE 2012, 7: e35471. PMID: 22509412, PMCID: PMC3324552, DOI: 10.1371/journal.pone.0035471.Peer-Reviewed Original Research
2011
High CJD infectivity remains after prion protein is destroyed
Miyazawa K, Emmerling K, Manuelidis L. High CJD infectivity remains after prion protein is destroyed. Journal Of Cellular Biochemistry 2011, 112: 3630-3637. PMID: 21793041, PMCID: PMC3202053, DOI: 10.1002/jcb.23286.Peer-Reviewed Original ResearchConceptsTransmissible spongiform encephalopathy agentsSpongiform encephalopathy agentInfectious particlesHost prion proteinPK digestionTotal PrPCJD infectivityInfectious homogenatesPrion proteinBrain resultsHigh titersTitersCell infectivityCell-based assaysCell culture assaysInfectivityProteinase K treatmentInfectious formPrPBrainCulture assaysAssaysProteinSensitive formK treatmentReplication and spread of CJD, kuru and scrapie agents in vivo and in cell culture
Miyazawa K, Emmerling K, Manuelidis L. Replication and spread of CJD, kuru and scrapie agents in vivo and in cell culture. Virulence 2011, 2: 188-199. PMID: 21527829, PMCID: PMC3149681, DOI: 10.4161/viru.2.3.15880.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Culture TechniquesCell LineCreutzfeldt-Jakob SyndromeDisease Models, AnimalHumansImmunity, InnateKuruMicePrionsRodent DiseasesScrapieSheepTime FactorsConceptsGT1 cellsSporadic CJDTSE agentsScrapie agentAgent-specific patternsTransmissible spongiform encephalopathy agentsComplex innate immune responseSpongiform encephalopathy agentInnate immune responseHost prion proteinK scrapie agentHuman CJDCJD agentNeuropathological sequelaeBrain titersImmune responseHuman kuruClearance mechanismsCJDInhibitory effectEnvironmental agentsCell-based assaysKuruInfectious formDistinct incubation times
2010
Nuclease resistant circular DNAs copurify with infectivity in scrapie and CJD
Manuelidis L. Nuclease resistant circular DNAs copurify with infectivity in scrapie and CJD. Journal Of NeuroVirology 2010, 17: 131-145. PMID: 21165784, DOI: 10.1007/s13365-010-0007-0.Peer-Reviewed Original ResearchMeSH KeywordsAcinetobacterAmino Acid SequenceAnimalsBase SequenceBrainConserved SequenceCreutzfeldt-Jakob SyndromeCricetinaeDeoxyribonucleasesDNA HelicasesDNA, CircularDNA, MitochondrialDNA-Directed DNA PolymeraseElectrophoresis, Agar GelGenome, BacterialHumansMiceMolecular Sequence DataNeuroblastomaNeurodegenerative DiseasesPlasmidsPolymerase Chain ReactionPrionsScrapieTumor Cells, Cultured
2009
The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents
Manuelidis L, Chakrabarty T, Miyazawa K, Nduom NA, Emmerling K. The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 13529-13534. PMID: 19633190, PMCID: PMC2715327, DOI: 10.1073/pnas.0905825106.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCattleCells, CulturedCreutzfeldt-Jakob SyndromeGeographyHumansKuruMiceNeuronsPrionsScrapieSheepTime FactorsTissue Culture TechniquesConceptsSporadic Creutzfeldt-Jakob diseaseCreutzfeldt-Jakob diseaseBovine spongiform encephalopathyBSE agentHuman sporadic Creutzfeldt-Jakob diseaseInfectious agentsEpidemic bovine spongiform encephalopathyTSE agentsScrapie agentTransmissible spongiform encephalopathy agentsSpongiform encephalopathy agentKuru agentLymphoreticular involvementBrain neuropathologySporadic CJDGT1 cellsNormal miceInfected humansNeurodegenerative diseasesDiseaseViral receptorsInfectious neurodegenerative diseasesSheep scrapieSpongiform encephalopathiesHigh levels
2008
Strain‐specific viral properties of variant Creutzfeldt–Jakob disease (vCJD) are encoded by the agent and not by host prion protein
Manuelidis L, Liu Y, Mullins B. Strain‐specific viral properties of variant Creutzfeldt–Jakob disease (vCJD) are encoded by the agent and not by host prion protein. Journal Of Cellular Biochemistry 2008, 106: 220-231. PMID: 19097123, PMCID: PMC2762821, DOI: 10.1002/jcb.21988.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCattleCreutzfeldt-Jakob SyndromeCricetinaeHumansPrion DiseasesPrionsSerial PassageSheepSpecies SpecificityConceptsVariant Creutzfeldt-Jakob diseaseBovine spongiform encephalopathyTransmissible spongiform encephalopathiesVCJD agentTSE strainsInfectious agentsNeuronal culturesEpidemic bovine spongiform encephalopathyMost viral infectionsCreutzfeldt-Jakob diseaseSpongiform encephalopathiesHost prion proteinHost PrP.Human CJDRegional neuropathologyVCJD brainPrion proteinSheep scrapie agentVariant CJDStrain-specific characteristicsBSE strainBrain homogenatesViral infectionPrimate brainScrapie agent
2001
Blood borne transit of CJD from brain to gut at early stages of infection
Radebold K, Chernyak M, Martin D, Manuelidis L. Blood borne transit of CJD from brain to gut at early stages of infection. BMC Infectious Diseases 2001, 1: 20. PMID: 11716790, PMCID: PMC59894, DOI: 10.1186/1471-2334-1-20.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCreutzfeldt-Jakob SyndromeDigestive SystemDisease Models, AnimalMicePrPSc ProteinsConceptsCreutzfeldt-Jakob diseaseIC inoculationAbnormal PrPCJD agentTransmissible spongiform encephalopathiesLymphoreticular tissuesEnteric infectionsIp inoculationPrP accumulationSpinal cordPeripheral tissuesGastrointestinal tractInfectious inoculumLymphatic drainageInfectious agentsVascular routeCell infectivityInfectionTerminal stageDiseaseSpongiform encephalopathiesDifferent organsBrainDaysProgressive appearance
1998
Cleaning CJD-contaminated instruments.
Manuelidis L. Cleaning CJD-contaminated instruments. Science 1998, 281: 1961. PMID: 9767039, DOI: 10.1126/science.281.5385.1961c.Peer-Reviewed Original ResearchCreutzfeldt-Jakob SyndromeDisinfectantsEquipment ContaminationGuanidinesHumansSurgical InstrumentsThiocyanates
1983
Scrapie-associated fibrils in Creutzfeldt–Jakob disease
Merz P, Somerville R, Wisniewski H, Manuelidis L, Manuelidis E. Scrapie-associated fibrils in Creutzfeldt–Jakob disease. Nature 1983, 306: 474-476. PMID: 6358899, DOI: 10.1038/306474a0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCreutzfeldt-Jakob SyndromeCricetinaeGuinea PigsHumansMiceScrapieSheepSpleenConceptsScrapie associated fibrilsCreutzfeldt-Jakob diseaseCentral nervous systemTitre of infectivityScrapie of sheepInfected brainPathological responseProgressive degenerationNervous systemSynaptosomal preparationsInfectious agentsBrain fractionsTransmissible encephalopathiesDiseaseHuman casesSpleen extractsAbnormal fibrilsSusceptible hostsScrapieClose associationDifferent tissuesEncephalopathyAgentsDegenerationTitres
1978
TRANSMISSION, FROM MAN TO HAMSTER, OF CREUTZFELDT-JAKOB DISEASE WITH CLINICAL RECOVERY
Manuelidis E, Manuelidis L, Pincus J, Collins W. TRANSMISSION, FROM MAN TO HAMSTER, OF CREUTZFELDT-JAKOB DISEASE WITH CLINICAL RECOVERY. The Lancet 1978, 312: 40-42. PMID: 78232, DOI: 10.1016/s0140-6736(78)91345-4.Peer-Reviewed Original ResearchAnimalsCreutzfeldt-Jakob SyndromeCricetinaeGuinea PigsHippocampusHumansMaleMesocricetusMiddle AgedSpinal Cord
1976
Serial propagation of Creutzfeldt-Jakob disease in guinea pigs.
Manuelidis E, Kim J, Angelo J, Manuelidis L. Serial propagation of Creutzfeldt-Jakob disease in guinea pigs. Proceedings Of The National Academy Of Sciences Of The United States Of America 1976, 73: 223-227. PMID: 1108016, PMCID: PMC335873, DOI: 10.1073/pnas.73.1.223.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCreutzfeldt-Jakob SyndromeDisease Models, AnimalGuinea PigsHumansHydrocephalusThalamusVisual CortexConceptsCreutzfeldt-Jakob diseaseGuinea pigsSubacute spongiform virus encephalopathiesSpongiform virus encephalopathiesHydrocephalus ex vacuoSubcortical gray structuresSerial propagationPercent takeNeuronal destructionCerebral atrophyNeuronal lossCerebral cortexEx vacuoGray structuresDiseaseSubsequent passagesPigsMorbidityAtrophyEncephalopathyIncubation periodInfectionMortalityCortexSymptomatology