1989
Mechanism of Membrane Anchoring Affects Polarized Expression of Two Proteins in MDCK Cells
Brown D, Crise B, Rose J. Mechanism of Membrane Anchoring Affects Polarized Expression of Two Proteins in MDCK Cells. Science 1989, 245: 1499-1501. PMID: 2571189, DOI: 10.1126/science.2571189.Peer-Reviewed Original ResearchConceptsMembrane anchorageGPI anchorBasolateral plasma membrane domainsSorting of proteinsPlasma membrane domainsPolarized epithelial cellsClass of proteinsBasolateral surfaceVesicular stomatitis virus glycoproteinMembrane anchoringCytoplasmic domainMembrane domainsMembrane proteinsPolypeptide sequenceTransport signalVSV GPolarized expressionMDCK cellsApical expressionProteinApical surfaceEpithelial cellsVirus glycoproteinPlacental alkaline phosphataseExpression
1987
Replacement of the cytoplasmic domain alters sorting of a viral glycoprotein in polarized cells.
Puddington L, Woodgett C, Rose J. Replacement of the cytoplasmic domain alters sorting of a viral glycoprotein in polarized cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 2756-2760. PMID: 3033661, PMCID: PMC304737, DOI: 10.1073/pnas.84.9.2756.Peer-Reviewed Original ResearchConceptsCytoplasmic domainG proteinsPlasma membraneVesicular stomatitis virusNormal cytoplasmic domainIntegral membrane proteinsPolarized epithelial cellsVSV G proteinApical plasma membraneBasolateral plasma membraneBasolateral membraneCanine kidney cell lineMadin-Darby canine kidney (MDCK) cell lineIndirect immunofluorescence microscopyMembrane proteinsKidney cell lineDomain altersPolarized expressionImmunofluorescence microscopyBasolateral surfaceProteinStomatitis virusCell linesViral glycoproteinsEpithelial cells