2001
Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons
Sasaki M, Honmou O, Akiyama Y, Uede T, Hashi K, Kocsis J. Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons. Glia 2001, 35: 26-34. PMID: 11424189, PMCID: PMC2605363, DOI: 10.1002/glia.1067.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBeta-GalactosidaseBone Marrow TransplantationCells, CulturedEthidiumGlial Fibrillary Acidic ProteinImmunohistochemistryMiceMice, TransgenicMyeloid Progenitor CellsNerve Fibers, MyelinatedNerve RegenerationNeurogliaRatsRats, WistarRecovery of FunctionSpinal CordSpinal Cord InjuriesConceptsBone marrow cellsSpinal cordMyelin-forming cellsMarrow cellsDemyelinated rat spinal cordRat spinal cord axonsDorsal column lesionBone marrow cell fractionRat spinal cordX-irradiation treatmentSpinal cord axonsLacZ transgenic miceSchwann cell myelinationCell fractionCell transplantation techniquesDorsal funiculusPeripheral patternTransgenic miceTransplantation techniquesHematopoietic stem cellsIsolated cell fractionsCordFemoral bonePrecursor cellsTransplantationTransplantation of Clonal Neural Precursor Cells Derived from Adult Human Brain Establishes Functional Peripheral Myelin in the Rat Spinal Cord
Akiyama Y, Honmou O, Kato T, Uede T, Hashi K, Kocsis J. Transplantation of Clonal Neural Precursor Cells Derived from Adult Human Brain Establishes Functional Peripheral Myelin in the Rat Spinal Cord. Experimental Neurology 2001, 167: 27-39. PMID: 11161590, DOI: 10.1006/exnr.2000.7539.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsBrainBrain Tissue TransplantationCell DifferentiationCells, CulturedCerebral VentriclesClone CellsDemyelinating DiseasesFemaleGenes, ReporterHumansIntermediate Filament ProteinsMaleMiddle AgedMyelin SheathNerve Tissue ProteinsNestinNeural ConductionNeuronsRadiation Injuries, ExperimentalRatsRats, WistarSpinal CordStem Cell TransplantationStem CellsTransplantation, HeterologousConceptsAdult human brainRat spinal cordNestin-positive cellsNeural precursor cellsSpinal cordAdult rat spinal cordNormal conduction velocityHuman brainMitogen withdrawalSpinal cord resultsPrecursor cellsGlia-like cellsSchwann cell myelinationNeural progenitor cellsFunctional remyelinationP0 immunoreactivityRemyelinated axonsExtensive remyelinationCord resultsAnterior hornLateral ventricleSubventricular zoneMajority of cellsMyelin patternSchwann cells
2000
Xenotransplantation of transgenic pig olfactory ensheathing cells promotes axonal regeneration in rat spinal cord
Imaizumi T, Lankford K, Burton W, Fodor W, Kocsis J. Xenotransplantation of transgenic pig olfactory ensheathing cells promotes axonal regeneration in rat spinal cord. Nature Biotechnology 2000, 18: 949-953. PMID: 10973214, PMCID: PMC2605371, DOI: 10.1038/79432.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedAxonsCD59 AntigensCell SeparationElectrophysiologyFlow CytometryFluorescent Antibody Technique, IndirectHumansImmunosuppression TherapyModels, BiologicalOlfactory NerveRatsRats, WistarRegenerationSchwann CellsSciatic NerveSpinal CordSwineTransgenesTransplantation, HeterologousConceptsAxonal regenerationSpinal cordSchwann cellsImpulse conductionLesion-control ratsDorsal column lesionTransplantation of olfactoryRat spinal cordConduction velocity measurementsComplement inhibitory proteinsHyperacute responseRegenerated axonsImmunosuppressed ratsTransection siteLesion sitePeripheral patternHost tractCordNormal axonsDonor cellsAxonsInhibitory proteinRatsDonor cell typeTransgenic pigs[Comparison of myelin-forming cells as candidates for therapeutic transplantation in demyelinated CNS axons].
Imaizumi T, Lankford K, Kocsis J, Sasaki M, Akiyama Y, Hashi K. [Comparison of myelin-forming cells as candidates for therapeutic transplantation in demyelinated CNS axons]. Brain And Nerve 脳と神経 2000, 52: 609-15. PMID: 10934721.Peer-Reviewed Original ResearchConceptsDemyelinated CNS axonsSchwann cellsBrain cellsSpinal cordConduction velocityCNS axonsAdult rat spinal cordFronto-temporal lobesRat spinal cordAdult Schwann cellsDemyelination of axonsMyelin-forming cellsBlock of conductionAdult olfactoryDemyelinated diseasesDemyelinated axonsLess myelinationHistological examinationCNS tissueTherapeutic approachesStimulus trainsDay 28Transplanted OECsAmplitude decrementRemyelinationTransplantation of human olfactory ensheathing cells elicits remyelination of demyelinated rat spinal cord
Kato T, Honmou O, Uede T, Hashi K, Kocsis J. Transplantation of human olfactory ensheathing cells elicits remyelination of demyelinated rat spinal cord. Glia 2000, 30: 209-218. PMID: 10756071, PMCID: PMC2605375, DOI: 10.1002/(sici)1098-1136(200005)30:3<209::aid-glia1>3.0.co;2-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCation Transport ProteinsCell SizeCells, CulturedDemyelinating DiseasesDNA ProbesFemaleFungal ProteinsHumansIn Situ HybridizationMembrane Transport ProteinsMicroscopy, ElectronMiddle AgedMyelin SheathNeurogliaOlfactory NerveRatsRats, WistarSaccharomyces cerevisiae ProteinsSpinal CordConceptsSpinal cordDemyelinated rat spinal cordHuman olfactoryImmunosuppressed adult ratsDemyelinated spinal cordAdult mammalian CNSRat spinal cordExtensive remyelinationHuman OECsDemyelinated axonsOlfactory nervePeripheral typeSchwann cellsAdult ratsLesion siteMammalian CNSMyelin sheathRemyelinationCordAxonsSitu hybridizationSimilar numberOlfactoryCellsLarge nucleiTransplantation of olfactory ensheathing cells or Schwann cells restores rapid and secure conduction across the transected spinal cord
Imaizumi T, Lankford K, Kocsis J. Transplantation of olfactory ensheathing cells or Schwann cells restores rapid and secure conduction across the transected spinal cord. Brain Research 2000, 854: 70-78. PMID: 10784108, DOI: 10.1016/s0006-8993(99)02285-4.Peer-Reviewed Original ResearchConceptsRegenerated axonsCell transplantationSpinal cordSchwann cellsTransection siteIsolated spinal cord preparationSpinal cord preparationTransplantation of olfactoryRat spinal cordSpinal cord axonsConduction velocity measurementsTransplantation of cellsCord preparationDorsal columnsAxonal regenerationAxon areaTransplantationImpulse conductionHost tractElectrophysiological recordingsAxonsNormal axonsDonor cellsNeuronal sourcesCord
1999
The role of voltage-gated Ca2+ channels in anoxic injury of spinal cord white matter
Imaizumi T, Kocsis J, Waxman S. The role of voltage-gated Ca2+ channels in anoxic injury of spinal cord white matter. Brain Research 1999, 817: 84-92. PMID: 9889329, DOI: 10.1016/s0006-8993(98)01214-1.Peer-Reviewed Original ResearchConceptsVoltage-gated Ca2Spinal cord axonsAnoxic injuryDorsal columnsR-type voltage-gated Ca2N-type calcium channelsSpinal cord white matterRat dorsal columnsDorsal column axonsR-type Ca2Rat spinal cordCord white matterT-type channelsInflux of Ca2Dose-dependent mannerLoss of conductionAxonal conductionSpinal cordChannel blockersCalcium channelsSurface stimulationWhite matterPerfusion solutionInjuryGlass microelectrodes
1998
Transplanted Olfactory Ensheathing Cells Remyelinate and Enhance Axonal Conduction in the Demyelinated Dorsal Columns of the Rat Spinal Cord
Imaizumi T, Lankford K, Waxman S, Greer C, Kocsis J. Transplanted Olfactory Ensheathing Cells Remyelinate and Enhance Axonal Conduction in the Demyelinated Dorsal Columns of the Rat Spinal Cord. Journal Of Neuroscience 1998, 18: 6176-6185. PMID: 9698311, PMCID: PMC2605360, DOI: 10.1523/jneurosci.18-16-06176.1998.Peer-Reviewed Original ResearchConceptsDorsal column axonsRat spinal cordSpinal cordRemyelinated axonsDorsal columnsAdult rat spinal cordExtent of remyelinationTransplantation of OECsSpinal cord lesionsCell injection siteQuantitative histological analysisFunctional remyelinationCord lesionsAxonal conductionNeonatal ratsFocal injectionsConduction blockSchwann cellsConduction velocityInjection siteElectrophysiological propertiesAction potentialsAxonsHistological analysisTransplantationResistance to anoxic injury in the dorsal columns of adult rat spinal cord following demyelination
Imaizumi T, Kocsis J, Waxman S. Resistance to anoxic injury in the dorsal columns of adult rat spinal cord following demyelination. Brain Research 1998, 779: 292-296. PMID: 9473700, DOI: 10.1016/s0006-8993(97)01171-2.Peer-Reviewed Original ResearchConceptsCompound action potentialDorsal columnsSpinal cordAction potentialsWhite matterSpinal cord dorsal columnAdult rat spinal cordAdult spinal cordRat spinal cordMin of reoxygenationAdult white matterAction potential conductionControl ratsFocal demyelinationAnoxic injurySupramaximal stimulationOnset of anoxiaAdult ratsDemyelinationPotential conductionCordX-irradiationRatsGreater recoveryOnset
1992
Conduction properties of spinal cord axons in the myelin-deficient rat mutant
Utzschneider D, Black J, Kocsis J. Conduction properties of spinal cord axons in the myelin-deficient rat mutant. Neuroscience 1992, 49: 221-228. PMID: 1407548, DOI: 10.1016/0306-4522(92)90090-o.Peer-Reviewed Original ResearchConceptsDorsal column axonsMyelin-deficient rat mutantsSpinal cordFrequency-dependent conduction blockNormal age-matched ratsBrain slice chamberMyelin-deficient rat spinal cordAction potential dischargeRat mutantAge-matched ratsRat spinal cordSpinal cord axonsAction potential conductionControl ratsDemyelinated axonsConduction blockConduction velocityRefractory periodPotential dischargeControl axonsSlice chamberPharmacological resultsPotential conductionAxonsCompensatory mechanisms