2013
Paracrine Loop of Keratinocyte Proliferation and Directed Neuritic Outgrowth in a Neuroepithelial Coculture
Radtke C, Rennekampff H, Reimers K, Vogt P, Kocsis J. Paracrine Loop of Keratinocyte Proliferation and Directed Neuritic Outgrowth in a Neuroepithelial Coculture. Annals Of Plastic Surgery 2013, Publish Ahead of Print: &na;. DOI: 10.1097/sap.0b013e318276d946.Peer-Reviewed Original ResearchNerve growth factorNeuritic outgrowthDRG neuronsHuman keratinocytesNeurotrophin nerve growth factorDorsal root ganglion neuronsCutaneous afferent axonsKeratinocyte proliferationAnti-NGF antibodyWound healingChronic nonhealing woundsProliferation of keratinocytesNGF levelsSkin innervationPrimary human keratinocytesNeurotrophic factorGanglion neuronsAfferent axonsParacrine loopNonhealing woundsMitogenic effectCell bodiesNeuritic processesGrowth factorNeurons
1999
Reduction in Potassium Currents in Identified Cutaneous Afferent Dorsal Root Ganglion Neurons After Axotomy
Everill B, Kocsis J. Reduction in Potassium Currents in Identified Cutaneous Afferent Dorsal Root Ganglion Neurons After Axotomy. Journal Of Neurophysiology 1999, 82: 700-708. PMID: 10444667, DOI: 10.1152/jn.1999.82.2.700.Peer-Reviewed Original ResearchConceptsDorsal root ganglion neuronsCutaneous afferent neuronsAfferent neuronsGanglion neuronsPotassium currentWhole-cell patch-clamp recordingsCell patch-clamp recordingsPrimary afferent neuronsEffects of axotomyPatch-clamp recordingsControl cellsAppropriate ion replacementAcute ligationNerve ligationDRG neuronsExcitability changesChannel blockersNeuronal excitabilityPharmacological componentsAxotomyNeuronsLigationBasis of sensitivityCellsBlockers
1998
Morphologically Identified Cutaneous Afferent DRG Neurons Express Three Different Potassium Currents in Varying Proportions
Everill B, Rizzo M, Kocsis J. Morphologically Identified Cutaneous Afferent DRG Neurons Express Three Different Potassium Currents in Varying Proportions. Journal Of Neurophysiology 1998, 79: 1814-1824. PMID: 9535950, PMCID: PMC2605378, DOI: 10.1152/jn.1998.79.4.1814.Peer-Reviewed Original ResearchConceptsDRG neuronsMedium-sized DRG neuronsDorsal root ganglion neuronsCutaneous afferent neuronsDifferent potassium currentsWhole-cell patch-clamp configurationOutward current componentPatch-clamp configurationAfferent neuronsFluoro-GoldGanglion neuronsRetrograde labelingConditioning prepulseEntire populationChannel blockersPotassium currentMyelinated axonsSustained currentNeuronsTest pulseDendrotoxinBasis of sensitivityIKCellsBlockersMechanisms of enhancement of neurite regeneration in vitro following a conditioning sciatic nerve lesion
Lankford K, Waxman S, Kocsis J. Mechanisms of enhancement of neurite regeneration in vitro following a conditioning sciatic nerve lesion. The Journal Of Comparative Neurology 1998, 391: 11-29. PMID: 9527536, PMCID: PMC2605358, DOI: 10.1002/(sici)1096-9861(19980202)391:1<11::aid-cne2>3.0.co;2-u.Peer-Reviewed Original ResearchConceptsDorsal root gangliaConditioning lesionNerve injuryNerve regenerationAffected dorsal root ganglionControl dorsal root gangliaDenervated peripheral nervePrior nerve injurySciatic nerve lesionCultured DRG neuronsSciatic nerve transectionPeripheral target tissuesPeripheral nerve stumpRapid nerve regenerationAbility of neuronsSecond axotomyNerve lesionsDRG neuronsNerve transectionNerve stumpRoot gangliaControl neuronsPeripheral nervesNerve tractsAdult rats
1997
Temporal variability of jun family transcription factor levels in peripherally or centrally transected adult rat dorsal root ganglia
Kenney A, Kocsis J. Temporal variability of jun family transcription factor levels in peripherally or centrally transected adult rat dorsal root ganglia. Brain Research 1997, 52: 53-61. PMID: 9450677, DOI: 10.1016/s0169-328x(97)00211-8.Peer-Reviewed Original ResearchConceptsDorsal root gangliaDorsal root transectionPeripheral axotomyDRG neuronsRoot transectionRoot gangliaProtein levelsAdult rat lumbar dorsal root gangliaRat lumbar dorsal root gangliaLumbar dorsal root gangliaAdult rat dorsal root gangliaRat dorsal root gangliaNeurotrophic factor administrationSpinal nerve transectionC-Jun protein levelsAdministration of NGFFactor protein levelsJunD protein levelsTarget disconnectionNerve transectionFactor administrationPeripheral branchesTransectionFactor levelsRapid elevation
1996
GABAA-receptor-mediated conductance and action potential waveform in cutaneous and muscle afferent neurons of the adult rat: differential expression and response to nerve injury
Oyelese A, Kocsis J. GABAA-receptor-mediated conductance and action potential waveform in cutaneous and muscle afferent neurons of the adult rat: differential expression and response to nerve injury. Journal Of Neurophysiology 1996, 76: 2383-2392. PMID: 8899611, PMCID: PMC2605353, DOI: 10.1152/jn.1996.76.4.2383.Peer-Reviewed Original ResearchConceptsGABAA receptor-mediated conductanceMuscle afferent neuronsCutaneous afferent neuronsAction potential waveformAfferent neuronsDorsal root gangliaCrush injuryAction potentialsL5 dorsal root gangliaWhole-cell patch-clamp recordingsCell patch-clamp recordingsInjury-induced plasticitySciatic nerve ligationPotential waveformDistal nerve stumpPatch-clamp recordingsGamma-aminobutyric acidPeak GABANerve ligationMuscle afferentsNerve injuryDRG neuronsInjured neuronsNerve stumpRoot gangliaMechanisms of Paresthesiae, Dysesthesiae, and Hyperesthesiae: Role of Na+ Channel Heterogeneity
Rizzo M, Kocsis J, Waxman S. Mechanisms of Paresthesiae, Dysesthesiae, and Hyperesthesiae: Role of Na+ Channel Heterogeneity. European Neurology 1996, 36: 3-12. PMID: 8719643, DOI: 10.1159/000117192.Peer-Reviewed Original ResearchConceptsAxonal injuryCutaneous afferentsDorsal root ganglion neuronsAction potential activityNormal sensory functionEctopic impulsesDRG neuronsClinical syndromeGanglion neuronsSensory functionMembrane excitabilityInjuryNerve impulsesDysesthesiaeChannel physiologyMolecular changesParesthesiaeAfferentsPreliminary evidenceNeuronsEctopicMolecular mechanismsSensory anatomyPotential activityPopulation
1995
Enhancement of GABAA receptor-mediated conductances induced by nerve injury in a subclass of sensory neurons
Oyelese A, Eng D, Richerson G, Kocsis J. Enhancement of GABAA receptor-mediated conductances induced by nerve injury in a subclass of sensory neurons. Journal Of Neurophysiology 1995, 74: 673-683. PMID: 7472373, PMCID: PMC2605359, DOI: 10.1152/jn.1995.74.2.673.Peer-Reviewed Original ResearchConceptsDRG neuronsAction potential durationMedium neuronsNerve injurySmall neuronsLarge neuronsAction potentialsGABA conductanceNerve ligationControl neuronsPotential durationAdult rat dorsal root ganglion neuronsGABAA receptor-mediated conductanceRat dorsal root ganglion neuronsWhole-cell patch-clamp recordingsCell patch-clamp recordingsDorsal root ganglion neuronsMean action potential durationShort-duration action potentialsControl DRG neuronsSciatic nerve ligationEffects of axotomyReceptor-mediated conductanceTraumatic nerve injuryPatch-clamp recordings
1994
Intracellular calcium mobilization and neurite outgrowth in mammalian neurons
Kocsis J, Rand M, Lankford K, Waxman S. Intracellular calcium mobilization and neurite outgrowth in mammalian neurons. Developmental Neurobiology 1994, 25: 252-264. PMID: 8195789, DOI: 10.1002/neu.480250306.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsCaffeineCalciumCalcium ChannelsCell CompartmentationCell DifferentiationCells, CulturedFemaleGanglia, SpinalGene Expression RegulationImage Processing, Computer-AssistedIntracellular FluidIon Channel GatingLasersMicroscopy, FluorescenceModels, BiologicalNeuritesNeuronsRatsRats, WistarTerpenesThapsigarginConceptsDepolarization-induced Ca2Calcium-induced calcium releaseDRG neuronsIntracellular Ca2Cultured adult rat dorsal root ganglion neuronsAdult rat dorsal root ganglion neuronsRat dorsal root ganglion neuronsNeurite outgrowthDorsal root ganglion neuronsIntact intracellular Ca2Intracellular calcium mobilizationIndicator dye fluo-3Nuclear Ca2Dye Fluo-3Endoplasmic reticulum Ca2Ganglion neuronsCalcium mobilizationDependent kinase IICalcium releaseFluo-3NeuronsMammalian neuronsReticulum Ca2Elevated Ca2Elicit neurite outgrowthNuclear and cytoplasmic Ca2+ signals in developing rat dorsal root ganglion neurons studied in excised tissue
Utzschneider D, Rand M, Waxman S, Kocsis J. Nuclear and cytoplasmic Ca2+ signals in developing rat dorsal root ganglion neurons studied in excised tissue. Brain Research 1994, 635: 231-237. PMID: 8173960, DOI: 10.1016/0006-8993(94)91444-3.Peer-Reviewed Original ResearchConceptsDorsal root ganglion neuronsEmbryonic DRG neuronsDepolarization-induced Ca2DRG neuronsGanglion neuronsRat dorsal root ganglion neuronsAdult dorsal root ganglion neuronsCytoplasmic Ca2Sensitive fluorescent dye fluo-3Rat DRG neuronsDorsal root gangliaExcised dorsal root gangliaFluorescent dye fluo-3Postnatal time periodsElevated extracellular potassiumEarly postnatal time periodsBrief electrical stimuliNuclear Ca2Dye Fluo-3Neonatal gangliaNeonatal neuronsTarget innervationRoot gangliaAdult neuronsEmbryonic neurons
1992
Mutual excitation among dorsal root ganglion neurons in the rat
Utzschneider D, Kocsis J, Devor M. Mutual excitation among dorsal root ganglion neurons in the rat. Neuroscience Letters 1992, 146: 53-56. PMID: 1475049, DOI: 10.1016/0304-3940(92)90170-c.Peer-Reviewed Original ResearchConceptsDorsal root gangliaDorsal rootsDorsal root ganglion neuronsPrimary sensory neuronsExcised dorsal root gangliaNerve injuryDRG neuronsGanglion neuronsMost neuronsRoot gangliaSensory abnormalitiesIntracellular recordingsDR stimulationRepetitive stimulationSensory neuronsTransient riseNeighboring neuronsNeuronsStimulationSimilar kineticsMutual excitationGangliaInjuryRatsAbnormalities