2002
Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential
Guerrero G, Siegel M, Roska B, Loots E, Isacoff E. Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential. Biophysical Journal 2002, 83: 3607-3618. PMID: 12496128, PMCID: PMC1302436, DOI: 10.1016/s0006-3495(02)75361-7.Peer-Reviewed Original ResearchMeSH KeywordsBiosensing TechniquesFluorescence Resonance Energy TransferGreen Fluorescent ProteinsLuminescent ProteinsMembrane PotentialsMicroscopy, FluorescenceMutagenesis, Site-DirectedNeuronsOptics and PhotonicsPotassium ChannelsPotassium Channels, Voltage-GatedProtein EngineeringRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsConceptsDetector domainDual-wavelength measurementVoltage-gated ShakerWavelength measurementsOptical readoutOptical sensorsSpectral shiftElectrical signalsIntensity changesDistinct spectraFlashesReadoutSpectraFluorescence intensity changesVoltage dependenceDependenceVoltage rangeMeasurementsSensorsDistinct voltageVoltageDynamicsShift
2001
Genetically encoded optical sensors of neuronal activity and cellular function
Guerrero G, Isacoff E. Genetically encoded optical sensors of neuronal activity and cellular function. Current Opinion In Neurobiology 2001, 11: 601-607. PMID: 11595495, DOI: 10.1016/s0959-4388(00)00256-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGenetic EngineeringGreen Fluorescent ProteinsHumansIndicators and ReagentsLuminescent ProteinsNeuronsOptics and PhotonicsSignal TransductionConceptsCellular signalsFluorescent proteinSpecific cellular signalsCellular functionsIntracellular messengerSpecific mutationsStructural rearrangementsFP fluorescenceCyclic nucleotidesNeurotransmitter releaseNew insightsProteinMembrane voltageOptical sensorsNeural circuitsNucleotidesFluorescenceMessengerMutationsRearrangementNitric oxideSignalsChemical environmentNeuronal activityFusion