Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential
Guerrero G, Siegel M, Roska B, Loots E, Isacoff E. Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential. Biophysical Journal 2002, 83: 3607-3618. PMID: 12496128, PMCID: PMC1302436, DOI: 10.1016/s0006-3495(02)75361-7.Peer-Reviewed Original ResearchMeSH KeywordsBiosensing TechniquesFluorescence Resonance Energy TransferGreen Fluorescent ProteinsLuminescent ProteinsMembrane PotentialsMicroscopy, FluorescenceMutagenesis, Site-DirectedNeuronsOptics and PhotonicsPotassium ChannelsPotassium Channels, Voltage-GatedProtein EngineeringRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsConceptsDetector domainDual-wavelength measurementVoltage-gated ShakerWavelength measurementsOptical readoutOptical sensorsSpectral shiftElectrical signalsIntensity changesDistinct spectraFlashesReadoutSpectraFluorescence intensity changesVoltage dependenceDependenceVoltage rangeMeasurementsSensorsDistinct voltageVoltageDynamicsShift