2009
Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy
Wang L, Sigworth FJ. Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy. Nature 2009, 461: 292-295. PMID: 19718020, PMCID: PMC2797367, DOI: 10.1038/nature08291.Peer-Reviewed Original ResearchConceptsVoltage sensor domainNative membrane environmentMembrane proteinsMembrane environmentChannel voltage-sensor domainElectron cryomicroscopyVoltage-activated potassium channelsSingle-particle electron cryomicroscopyIndividual protein particlesPotassium channelsBK potassium channelsLarge conductance calciumLipid membranesX-ray crystal structureBK channelsStructural studiesProtein particlesProteinCryomicroscopyCrystal structureFormation of crystalsDomainStructureCrystalsChannels
2006
Using cryo-EM to measure the dipole potential of a lipid membrane
Wang L, Bose PS, Sigworth FJ. Using cryo-EM to measure the dipole potential of a lipid membrane. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 18528-18533. PMID: 17116859, PMCID: PMC1693696, DOI: 10.1073/pnas.0608714103.Peer-Reviewed Original ResearchConceptsDipole potentialLipid membranesPhase-contrast imagingMolecular dynamics calculationsLipid bilayer membranesIon translocation ratesAtomic potentialLipid moleculesLipid monolayersBilayer membranesDynamics calculationsProbe methodSurface potentialAnionsDiphytanoylphosphatidylcholineCationsElectronsImage intensityAbsolute valueMembraneMoleculesConformationLarge permeabilityMonolayersBilayers