2007
Dimerization of FIR upon FUSE DNA binding suggests a mechanism of c‐myc inhibition
Crichlow GV, Zhou H, Hsiao HH, Frederick KB, Debrosse M, Yang Y, Folta-Stogniew EJ, Chung HJ, Fan C, De La Cruz EM, Levens D, Lolis E, Braddock D. Dimerization of FIR upon FUSE DNA binding suggests a mechanism of c‐myc inhibition. The EMBO Journal 2007, 27: 277-289. PMID: 18059478, PMCID: PMC2206118, DOI: 10.1038/sj.emboj.7601936.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCrystallography, X-RayDimerizationDNADNA HelicasesDNA-Binding ProteinsDrosophila ProteinsGene Expression RegulationHumansMagnetic Resonance SpectroscopyMolecular Sequence DataPromoter Regions, GeneticProtein BindingProto-Oncogene Proteins c-mycRepressor ProteinsRNA Splicing FactorsRNA-Binding ProteinsTranscription Factor TFIIHConceptsRRM domainDNA bindingFirst RRM domainSecond RRM domainC-myc transcriptional controlSite-directed mutationsDNA upstreamTranscriptional controlInfluences transcriptionC-Myc inhibitionNucleic acid recognitionPromoter sitesP1 promoterAnalogous mutationCell homeostasisC-MycTFIIHProteinLight scattering revealBinding sitesDNATranscriptionSingle strandsMutationsSize exclusion chromatography
2006
Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors
Folta-Stogniew E. Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors. Methods In Molecular Biology 2006, 328: 97-112. PMID: 16785643, DOI: 10.1385/1-59745-026-x:97.Peer-Reviewed Original ResearchConceptsSize exclusion chromatographyRefractive indexMolar massStatic laser lightLaser lightOligomeric stateRefractive index detectorProtein-detergent complexesLight scatteringIndex detectorUltraviolet detectionDetectorFractionation stepsNon-ionic detergentAnomalous behaviorGlycosylated proteinsChromatographyNative proteinScatteringMassPolypeptide chainStateRI analysisRI approachUniversal approach
2003
Trimeric Subunit Stoichiometry of the Glutamate Transporters from Bacillus caldotenax and Bacillus stearothermophilus †
Yernool D, Boudker O, Folta-Stogniew E, Gouaux E. Trimeric Subunit Stoichiometry of the Glutamate Transporters from Bacillus caldotenax and Bacillus stearothermophilus †. Biochemistry 2003, 42: 12981-12988. PMID: 14596613, DOI: 10.1021/bi030161q.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid Transport System X-AGBacillusChromatography, GelCross-Linking ReagentsElectrophoresis, Polyacrylamide GelGenetic VectorsGeobacillus stearothermophilusGlutaralLasersLiposomesMolecular Sequence DataMolecular WeightProtein SubunitsProtein TransportScattering, RadiationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpectrophotometry, UltravioletConceptsSubunit stoichiometryBacillus caldotenaxMembrane proteinsPolytopic integral membrane proteinPrimary structureAmino acid sequence relationshipsGlutamate transportersBacillus stearothermophilusKingdoms of lifeIntegral membrane proteinsNeutral amino acid transporterTrimeric quaternary structureNative molecular massDetergent-solubilized stateAmino acid transportersSize exclusion chromatographyB. caldotenaxEukaryotic organismsHigh-affinity glutamate transportersEukaryotic transportersQuaternary organizationAqueous solutionNutrient uptakeLife formsDetergent micelles
1999
Determination of molecular masses of proteins in solution: Implementation of an HPLC size exclusion chromatography and laser light scattering service in a core laboratory.
Folta-Stogniew E, Williams KR. Determination of molecular masses of proteins in solution: Implementation of an HPLC size exclusion chromatography and laser light scattering service in a core laboratory. Journal Of Biomolecular Techniques 1999, 10: 51-63. PMID: 19499008, PMCID: PMC2291588.Peer-Reviewed Original ResearchRefractive indexLaser lightSEC/LSEmission spectraAbsorption spectraLight scatteringSize exclusion chromatographyInstrument configurationLS experimentsProtein oligomerizationNative proteinRI detectorMolecular massNonglobular shapeSpectraMolecular weightProteinHPLC size exclusion chromatographyMW determinationLaserLightScatteringExclusion chromatographyProtein standardsDetector