1999
The selC‐associated SHI‐2 pathogenicity island of Shigella flexneri
Moss J, Cardozo T, Zychlinsky A, Groisman E. The selC‐associated SHI‐2 pathogenicity island of Shigella flexneri. Molecular Microbiology 1999, 33: 74-83. PMID: 10411725, DOI: 10.1046/j.1365-2958.1999.01449.x.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsChromosome MappingChromosomes, BacterialCloning, MolecularColicinsDNA Transposable ElementsDNA, BacterialEnterobacteriaceaeEscherichia coliGenes, BacterialHydroxamic AcidsIntegrasesOperonSequence AlignmentSequence Homology, Nucleic AcidShigella flexneriSpecies SpecificityTransposasesVirulenceConceptsPathogenicity islandSHI-2Different pathogenicity islandsChromosomal gene clusterRelated non-pathogenic speciesMultiple mobile genetic elementsMobile genetic elementsNon-pathogenic speciesSite of integrationTRNA genesSelC locusTRNA locusGene clusterNovel proteinGenetic elementsKb downstreamSiderophore systemStressful environmentsEscherichia coliVirulence factorsEnterohaemorrhagic E. coliE. coliUropathogenic Escherichia coliSalmonella entericaGenes
1995
Transcriptional autoregulation of the Salmonella typhimurium phoPQ operon
Soncini FC, Véscovi EG, Groisman EA. Transcriptional autoregulation of the Salmonella typhimurium phoPQ operon. Journal Of Bacteriology 1995, 177: 4364-4371. PMID: 7543474, PMCID: PMC177185, DOI: 10.1128/jb.177.15.4364-4371.1995.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsBase SequenceCloning, MolecularDNA Transposable ElementsGene Expression Regulation, BacterialHomeostasisLac OperonModels, GeneticMolecular Sequence DataMutationNucleic Acid ConformationOperonPromoter Regions, GeneticRNA, BacterialRNA, MessengerSalmonella typhimuriumTranscription FactorsTranscription, GeneticConceptsPhoPQ operonTranscriptional fusionsTranscriptional autoregulationPhoP-PhoQTwo-component regulatory systemAbsence of PhoPTranscriptional regulator PhoPExpression of phoPPhospho-PhoPPhoQ proteinPhoP proteinRegulator PhoPEnvironmental signalsDifferent transcriptsIndependent promotersPosttranscriptional mechanismsOperonPhoPPhoQGenesRegulatory systemBasal levelsProtein levelsProteinPromoter
1994
A Salmonella protein that is required for resistance to antimicrobial peptides and transport of potassium.
Parra‐Lopez C, Lin R, Aspedon A, Groisman EA. A Salmonella protein that is required for resistance to antimicrobial peptides and transport of potassium. The EMBO Journal 1994, 13: 3964-3972. PMID: 8076592, PMCID: PMC395316, DOI: 10.1002/j.1460-2075.1994.tb06712.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceBiological TransportCarrier ProteinsCloning, MolecularDrug Resistance, MicrobialMelittenMembrane ProteinsMolecular Sequence DataNADPeptidesPotassiumProtaminesReceptor, trkARecombinant ProteinsRestriction MappingSalmonella typhimuriumSequence Analysis, DNASequence Homology, Amino AcidConceptsE. coli proteinsAntimicrobial peptidesMolecular genetic analysisAntimicrobial peptide protaminePutative transportersTransport of peptidesColi proteinsSingle mutantsSalmonella proteinsSame resistance pathwaysSAP mutantsHost defense moleculesGenetic analysisDefense moleculesLoci participateChannel proteinsExhibit hypersensitivityEfflux proteinsUptake systemResistance pathwaysMutantsEscherichia coliProteinTransport of potassiumHost tissues
1993
Molecular genetic analysis of a locus required for resistance to antimicrobial peptides in Salmonella typhimurium.
Parra‐Lopez C, Baer MT, Groisman EA. Molecular genetic analysis of a locus required for resistance to antimicrobial peptides in Salmonella typhimurium. The EMBO Journal 1993, 12: 4053-4062. PMID: 8223423, PMCID: PMC413698, DOI: 10.1002/j.1460-2075.1993.tb06089.x.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acid SequenceAnti-Bacterial AgentsATP-Binding Cassette TransportersBase SequenceBiological TransportCloning, MolecularDrug Resistance, MicrobialEnterobacteriaceaeGenes, BacterialMelittenModels, BiologicalMolecular Sequence DataOperonPeptidesProtaminesSalmonella typhimuriumSequence Analysis, DNASequence Homology, Amino AcidConceptsUptake of oligopeptidesSet of genesOpen reading frameMolecular genetic analysisMammalian mdrYeast STE6Wild-type plasmidOperon structurePeriplasmic componentPeptide pheromoneNovel transporterReading frameKb segmentSalmonella typhimuriumGenetic analysisKb mRNASmall cationic peptidesSuccessful pathogenAntimicrobial peptide melittinSequence analysisCassette familyEnteric bacteriaPeptide transportAntimicrobial peptidesCancer cellsMolecular, functional, and evolutionary analysis of sequences specific to Salmonella.
Groisman EA, Sturmoski MA, Solomon FR, Lin R, Ochman H. Molecular, functional, and evolutionary analysis of sequences specific to Salmonella. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 1033-1037. PMID: 8430070, PMCID: PMC45805, DOI: 10.1073/pnas.90.3.1033.Peer-Reviewed Original ResearchConceptsAtypical base compositionIndependent evolutionary eventsPhenotypic characteristicsOpen reading frameLac gene fusionsWild-type parentLysR familyEvolutionary eventsDeletion strainEvolutionary analysisTranscriptional regulatorsReading frameSalmonella genomeNucleotide sequenceBase compositionHorizontal transferDNA fragmentsGene fusionsBacterial speciesEnteric bacteriaStructural similaritySpeciesVirulenceSequenceUnprecedented array
1992
Horizontal transfer of a phosphatase gene as evidence for mosaic structure of the Salmonella genome.
Groisman EA, Saier MH, Ochman H. Horizontal transfer of a phosphatase gene as evidence for mosaic structure of the Salmonella genome. The EMBO Journal 1992, 11: 1309-1316. PMID: 1339343, PMCID: PMC556579, DOI: 10.1002/j.1460-2075.1992.tb05175.x.Peer-Reviewed Original ResearchMeSH KeywordsAcid PhosphataseAmino Acid SequenceBase CompositionBase SequenceChromosomes, BacterialCloning, MolecularCodonDNAEscherichia coliGenes, BacterialGenome, BacterialMolecular Sequence DataMosaicismPhylogenyPlasmidsRestriction MappingSalmonella typhimuriumSequence Homology, Nucleic AcidTransfectionConceptsBase compositionPhoN geneNon-specific acid phosphatase activityAtypical base compositionSpacing of genesOverall base compositionNon-specific acid phosphataseCodon usage patternsGram-negative speciesChromosome sizeBacterial genomesPhosphatase geneKb regionSalmonella chromosomeSalmonella genomeGenetic basisHorizontal transferAcid phosphatase activityTrinucleotide frequenciesHigh similarityGenomeGenesEscherichia coliPhosphatase activityOriT regionMolecular genetic analysis of the Escherichia coli phoP locus
Groisman EA, Heffron F, Solomon F. Molecular genetic analysis of the Escherichia coli phoP locus. Journal Of Bacteriology 1992, 174: 486-491. PMID: 1530848, PMCID: PMC205741, DOI: 10.1128/jb.174.2.486-491.1992.Peer-Reviewed Original ResearchConceptsDeduced amino acid sequenceIsogenic wild-type strainE. coli genomePhoP geneMajor virulence regulatorAmino acid sequenceWild-type strainTwo-component systemMolecular genetic analysisColi genomePhoP locusVirulence regulatorIntramacrophage survivalAcid sequenceGenetic analysisEnteric speciesPhoP mutantE. coliGenesMagainin 2Cationic peptidesPhagocytic cellsGenomeHomologMutants
1991
Genome mapping and protein coding region identification using bacteriophage Mu
Groisman E, Pagratis N, Casadaban M. Genome mapping and protein coding region identification using bacteriophage Mu. Gene 1991, 99: 1-7. PMID: 1827084, DOI: 10.1016/0378-1119(91)90026-8.Peer-Reviewed Original ResearchConceptsMu-like phagesT7 RNA polymeraseEscherichia coli KRecombinant DNA technologySpecialized promotersBacterial genesGenome mappingRNA polymeraseBacteriophage T7Coli KHost translationBacteriophage MuMu phageDNA technologyMu vectorTransposonGenesPhagesPseudomonas aeruginosaChromosomesTranscriptionPolymerasePromoterOrganismsProtein
1988
Isolation, characterization, and cloning of a plasmid-borne gene encoding a phosphotransferase that confers high-level amikacin resistance in enteric bacilli
Gaynes R, Groisman E, Nelson E, Casadaban M, Lerner S. Isolation, characterization, and cloning of a plasmid-borne gene encoding a phosphotransferase that confers high-level amikacin resistance in enteric bacilli. Antimicrobial Agents And Chemotherapy 1988, 32: 1379-1384. PMID: 2848443, PMCID: PMC175872, DOI: 10.1128/aac.32.9.1379.Peer-Reviewed Original Research
1987
Cloning of genes from members of the family Enterobacteriaceae with mini-Mu bacteriophage containing plasmid replicons
Groisman EA, Casadaban MJ. Cloning of genes from members of the family Enterobacteriaceae with mini-Mu bacteriophage containing plasmid replicons. Journal Of Bacteriology 1987, 169: 687-693. PMID: 3542967, PMCID: PMC211834, DOI: 10.1128/jb.169.2.687-693.1987.Peer-Reviewed Original ResearchConceptsSensitive recipient strainFamily EnterobacteriaceaeHigh phage titersC. freundiiProteus mirabilisPlasmid repliconsCitrobacter freundiiS. typhimuriumDrug-resistant transductantsSpecific mutationsMu derivativesE. coliSalmonella typhimuriumEnterobacteriaceaeFreundiiRecipient strainPhage titerTyphimuriumEscherichia coli bacteriophageInfectionTitersIn vivo DNA cloning with a mini-Mu replicon cosmid and a helper lambda phage
Groisman E, Casadaban M. In vivo DNA cloning with a mini-Mu replicon cosmid and a helper lambda phage. Gene 1987, 51: 77-84. PMID: 2954879, DOI: 10.1016/0378-1119(87)90476-8.Peer-Reviewed Original Research
1986
Mini-mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing
Groisman EA, Casadaban MJ. Mini-mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing. Journal Of Bacteriology 1986, 168: 357-364. PMID: 3020001, PMCID: PMC213459, DOI: 10.1128/jb.168.1.357-364.1986.Peer-Reviewed Original Researchcrp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli
Cossart P, Groisman EA, Serre MC, Casadaban MJ, Gicquel-Sanzey B. crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli. Journal Of Bacteriology 1986, 167: 639-646. PMID: 3525518, PMCID: PMC212937, DOI: 10.1128/jb.167.2.639-646.1986.Peer-Reviewed Original ResearchConceptsE. coli crp geneNucleotide sequenceCatabolite gene activator proteinCRP geneGene activator proteinCrp gene productCorresponding amino acid sequencesE. coli CRPComplete nucleotide sequenceAmino acid divergenceEscherichia coli KAmino acid sequenceBase pair changesAmino acid differencesSalmonella typhimurium LT2S. typhimuriumRegulatory genesCodon usageActivator proteinRegulatory regionsGene productsAcid sequenceColi KPair changesAcid differences
1984
In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.
Groisman EA, Castilho BA, Casadaban MJ. In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1480-1483. PMID: 6324195, PMCID: PMC344860, DOI: 10.1073/pnas.81.5.1480.Peer-Reviewed Original Research