2006
Identification of the lipopolysaccharide modifications controlled by the Salmonella PmrA/PmrB system mediating resistance to Fe(III) and Al(III)
Nishino K, Hsu FF, Turk J, Cromie MJ, Wösten MM, Groisman EA. Identification of the lipopolysaccharide modifications controlled by the Salmonella PmrA/PmrB system mediating resistance to Fe(III) and Al(III). Molecular Microbiology 2006, 61: 645-654. PMID: 16803591, PMCID: PMC1618816, DOI: 10.1111/j.1365-2958.2006.05273.x.Peer-Reviewed Original ResearchMeSH KeywordsAluminumBacterial ProteinsBase SequenceDrug Resistance, BacterialEscherichia coli ProteinsGene Expression Regulation, BacterialIronLipid ALipopolysaccharidesMolecular Sequence DataMutationPeriplasmPhosphoric Monoester HydrolasesPhosphorylationPolymyxin BSalmonella typhimuriumSoil MicrobiologyTranscription FactorsConceptsPmrA/PmrB systemGram-negative bacterial speciesNon-host environmentsPmrA/PmrBWild-type strainSalmonella enterica serovar TyphimuriumEnterica serovar TyphimuriumOuter membraneLipopolysaccharide modificationBacterial speciesCovalent modificationResistance genesSerovar TyphimuriumOxygen-dependent killingPmrAEssential metalsHomeostatic mechanismsSalmonella survivalMutantsDephosphorylationGenesSpeciesProteinMajor constituentsIdentification
2004
Signal-dependent Binding of the Response Regulators PhoP and PmrA to Their Target Promoters in Vivo *
Shin D, Groisman EA. Signal-dependent Binding of the Response Regulators PhoP and PmrA to Their Target Promoters in Vivo *. Journal Of Biological Chemistry 2004, 280: 4089-4094. PMID: 15569664, DOI: 10.1074/jbc.m412741200.Peer-Reviewed Original ResearchConceptsResponse regulator PhoPPmrA proteinPhoP proteinRegulator PhoPTranscription of PhoPWild-type strainTarget promotersPromoter occupancyChromatin immunoprecipitationPromotes phosphorylationRegulatory regionsTarget genesPhoPValine residueLow Mg2GenesPhosphorylationPromoterProteinAsp 52TranscriptionSalmonella entericaVivoImmunoprecipitationMg2
2002
Fe(III)‐mediated cellular toxicity
Chamnongpol S, Dodson W, Cromie MJ, Harris ZL, Groisman EA. Fe(III)‐mediated cellular toxicity. Molecular Microbiology 2002, 45: 711-719. PMID: 12139617, DOI: 10.1046/j.1365-2958.2002.03041.x.Peer-Reviewed Original ResearchConceptsIsogenic wild-type strainSignal transduction systemWild-type strainGram-negative speciesPmrA mutantOuter membraneIron-mediated toxicityTransduction systemMicrobicidal activityBacterial survivalMajor regulatorCell deathPmrA geneIron homeostasisMutantsEscherichia coliSalmonella mutantsDeleterious metalsCellular toxicitySalmonella entericaExcellent biocatalystAnaerobic conditionsGenesRegulatorSpecies
2000
The regulatory protein PhoP controls susceptibility to the host inflammatory response in Shigella flexneri
Moss J, Fisher P, Vick B, Groisman E, Zychlinsky A. The regulatory protein PhoP controls susceptibility to the host inflammatory response in Shigella flexneri. Cellular Microbiology 2000, 2: 443-452. PMID: 11207599, DOI: 10.1046/j.1462-5822.2000.00065.x.Peer-Reviewed Original ResearchConceptsWild-type strainPhoP mutantPhoP/PhoQ systemTwo-component regulatory systemRegulatory protein PhoPHost cell phagosomesPhoP/PhoQKey virulence genesMutant bacteriaEnteric bacteriumPathogen escapeCell phagosomesHost cellsMutantsWild-type ShigellaPolymorphonuclear leucocytesRegulatory systemVirulence genesAntimicrobial moleculesAntimicrobial peptidesPhoPExtreme acidInflammatory responseEpithelial cellsPhagosomes
1996
Molecular basis of the magnesium deprivation response in Salmonella typhimurium: identification of PhoP-regulated genes
Soncini FC, Véscovi E, Solomon F, Groisman EA. Molecular basis of the magnesium deprivation response in Salmonella typhimurium: identification of PhoP-regulated genes. Journal Of Bacteriology 1996, 178: 5092-5099. PMID: 8752324, PMCID: PMC178303, DOI: 10.1128/jb.178.17.5092-5099.1996.Peer-Reviewed Original ResearchConceptsTranscription of PhoPPhoP-dependent mannerOpen reading frameLac gene fusionsWild-type strainPhoP-PhoQ systemTwo-component systemUDP-glucose dehydrogenaseSequence similarityPhoP-PhoQPhoPQ operonReading frameDeprivation responseFusion strainMolecular basisSalmonella typhimuriumPhoPDifferent proteinsSolid mediumGene fusionsUptake systemGenesNormal growthMgtASuch fusionIdentification of a pathogenicity island required for Salmonella survival in host cells.
Ochman H, Soncini FC, Solomon F, Groisman EA. Identification of a pathogenicity island required for Salmonella survival in host cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 7800-7804. PMID: 8755556, PMCID: PMC38828, DOI: 10.1073/pnas.93.15.7800.Peer-Reviewed Original ResearchConceptsPathogenicity islandType III secretion systemSalmonella typhimurium chromosomeWild-type levelsEpithelial cell invasionWild-type strainSPI genesSecretion systemPutative regulatorHost cellsSecretory apparatusCell invasionSecreted proteaseVirulence determinantsSpi(-) mutantsGenesProteinProteaseSalmonella survivalEnteric pathogensMutantsChromosomesIslandsCulture supernatantsForms of flagellin
1992
Molecular genetic analysis of the Escherichia coli phoP locus
Groisman EA, Heffron F, Solomon F. Molecular genetic analysis of the Escherichia coli phoP locus. Journal Of Bacteriology 1992, 174: 486-491. PMID: 1530848, PMCID: PMC205741, DOI: 10.1128/jb.174.2.486-491.1992.Peer-Reviewed Original ResearchConceptsDeduced amino acid sequenceIsogenic wild-type strainE. coli genomePhoP geneMajor virulence regulatorAmino acid sequenceWild-type strainTwo-component systemMolecular genetic analysisColi genomePhoP locusVirulence regulatorIntramacrophage survivalAcid sequenceGenetic analysisEnteric speciesPhoP mutantE. coliGenesMagainin 2Cationic peptidesPhagocytic cellsGenomeHomologMutants