2024
Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1
Tsemperouli M, Cheppali S, Rivera-Molina F, Chetrit D, Landajuela A, Toomre D, Karatekin E. Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1. Biophysical Journal 2024, 124: 1798-1814. PMID: 39719826, DOI: 10.1016/j.bpj.2024.12.023.Peer-Reviewed Original ResearchVesicle dockingSynaptotagmin-1C2 domainFusion triggeringCalcium sensor synaptotagmin 1Neuronal SNARE complexFusion pore dynamicsSensor synaptotagmin-1Domains of Syt1Neuroendocrine cell linePolybasic patchSNARE complexTriggers exocytosisFusion poreFusion eventsDense-core vesiclesSyt1Synaptic vesiclesPlasma membranePore regulationCalcium sensorNegatively charged phospholipidsBind calciumPore dynamicsCell lines
2006
Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging
Hua W, Sheff D, Toomre D, Mellman I. Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging. Journal Of Cell Biology 2006, 172: 1035-1044. PMID: 16567501, PMCID: PMC2063761, DOI: 10.1083/jcb.200512012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsCell LineCell MembraneCell PolarityDogsEndocytosisEpithelial CellsGlycoproteinsGlycosylphosphatidylinositolsGreen Fluorescent ProteinsKineticsLaser Scanning CytometryLuminescent ProteinsMembrane GlycoproteinsMembrane ProteinsNeural Cell Adhesion MoleculesProtein TransportRecombinant Fusion ProteinsTemperaturetrans-Golgi NetworkTransfectionTransport VesiclesViral Envelope ProteinsConceptsBasolateral membrane proteinsLive-cell imagingMembrane proteinsThree-dimensional live cell imagingGlycosylphosphatidylinositol-anchored proteinsVesicular stomatitis virus glycoproteinApical surfaceMadin-Darby canine kidney cellsCell imagingFilter-grown Madin-Darby canine kidney (MDCK) cellsEpithelial cellsBasolateral proteinsCanine kidney cellsTransport intermediatesVesicle dockingSecretory pathwayPlasma membraneVectorial insertionMembrane componentsJunctional complexesProteinRespective membranesKidney cellsVirus glycoproteinPathway
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