2021
FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria
Landajuela A, Braun M, Rodrigues CDA, Martínez-Calvo A, Doan T, Horenkamp F, Andronicos A, Shteyn V, Williams ND, Lin C, Wingreen NS, Rudner DZ, Karatekin E. FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria. PLOS Biology 2021, 19: e3001314. PMID: 34185788, PMCID: PMC8274934, DOI: 10.1371/journal.pbio.3001314.Peer-Reviewed Original ResearchConceptsMembrane fissionMembrane fission machineryMother cell membraneNegative-curvature lipidsMother cell cytoplasmUnique membrane topologyFission machineryMembrane scissionMembrane topologyLipid microdomainsMembrane necksFission proteinsLipid bindingMembrane curvatureFilamentous cellsBacillus subtilisFisBProtein BCell cytoplasmAcidic lipidsCell membraneCurvature sensingImmobile clustersIntrinsic abilityBacteria
2020
DNA Origami Post‐Processing by CRISPR‐Cas12a
Xiong Q, Xie C, Zhang Z, Liu L, Powell JT, Shen Q, Lin C. DNA Origami Post‐Processing by CRISPR‐Cas12a. Angewandte Chemie International Edition 2020, 59: 3956-3960. PMID: 31883145, PMCID: PMC7101258, DOI: 10.1002/anie.201915555.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Associated ProteinsDNAEndodeoxyribonucleasesConceptsDNA origami structuresCRISPR-Cas12aDNA origami devicesDNA origami designDNA origami techniqueNanomaterial fabricationFunctional nanodevicesDNA origamiScaffold strandTremendous promiseCutting-edge toolsVersatile toolNanodevicesNanostructuresFacileFabricationBiocompatibilityBiotechnologySequence specificityOrigamiEnzymatic methodMechanical propertiesStructural transformationDevicesIntricate structure