2024
Noncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin
Singh S, Siegler N, Pandey H, Yanir N, Popov M, Goldstein-Levitin A, Sadan M, Debs G, Zarivach R, Frank G, Kass I, Sindelar C, Zalk R, Gheber L. Noncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin. Science Advances 2024, 10: eadi1367. PMID: 38324691, PMCID: PMC10849588, DOI: 10.1126/sciadv.adi1367.Peer-Reviewed Original ResearchConceptsBidirectional motilityKinesin-5Plus-end-directed motilityKinesin-5 motorsCryo-EMC-terminal tailCryo-EM mapsPlus-end-directed kinesinCryo-electron microscopyDeletion mutantsNoncanonical interactionsIntracellular functionsCin8Cryo-electronMotor domainMotilityNonmotor domainsMutantsB-tubulinSingle-moleculeCell viabilityIn vivoIn vitroIn vitro experimentsLocal defects
2017
Structural basis of cooperativity in kinesin revealed by 3D reconstruction of a two-head-bound state on microtubules
Liu D, Liu X, Shang Z, Sindelar CV. Structural basis of cooperativity in kinesin revealed by 3D reconstruction of a two-head-bound state on microtubules. ELife 2017, 6: e24490. PMID: 28504639, PMCID: PMC5459574, DOI: 10.7554/elife.24490.Peer-Reviewed Original Research
2014
High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation
Shang Z, Zhou K, Xu C, Csencsits R, Cochran JC, Sindelar CV. High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation. ELife 2014, 3: e04686. PMID: 25415053, PMCID: PMC4383081, DOI: 10.7554/elife.04686.Peer-Reviewed Original Research
2012
Optimal noise reduction in 3D reconstructions of single particles using a volume-normalized filter
Sindelar CV, Grigorieff N. Optimal noise reduction in 3D reconstructions of single particles using a volume-normalized filter. Journal Of Structural Biology 2012, 180: 26-38. PMID: 22613568, PMCID: PMC3498508, DOI: 10.1016/j.jsb.2012.05.005.Peer-Reviewed Original ResearchConceptsParticle mapsOptimal noise reductionWiener filterError reduction schemeConventional Wiener filterNoise reductionNovel filterInversion methodNew filterHigh noise levelsParticle densityParticle volumeFilterParticlesNoise ratioNoise levelTheoretical analysisSingle particleAccurate implementationSpectral signalsSSNRInterior regionImage dataSignalsFourier inversion method
2010
An atomic-level mechanism for activation of the kinesin molecular motors
Sindelar CV, Downing KH. An atomic-level mechanism for activation of the kinesin molecular motors. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 4111-4116. PMID: 20160108, PMCID: PMC2840164, DOI: 10.1073/pnas.0911208107.Peer-Reviewed Original Research
2007
The beginning of kinesin's force-generating cycle visualized at 9-Å resolution
Sindelar CV, Downing KH. The beginning of kinesin's force-generating cycle visualized at 9-Å resolution. Journal Of Cell Biology 2007, 177: 377-385. PMID: 17470637, PMCID: PMC2064809, DOI: 10.1083/jcb.200612090.Peer-Reviewed Original ResearchConceptsSwitch II helixMicrotubule-binding proteinN-terminal extensionII helixNucleotide-free stateCryo-electron microscopySwitch IMicrotubule contactsResponse elementSingle-particle reconstructionConformational changesMicrotubulesActivation mechanismKinesinHigh-resolution characterizationHelixProtein
2003
Thermodynamic Properties of the Kinesin Neck-Region Docking to the Catalytic Core
Rice S, Cui Y, Sindelar C, Naber N, Matuska M, Vale R, Cooke R. Thermodynamic Properties of the Kinesin Neck-Region Docking to the Catalytic Core. Biophysical Journal 2003, 84: 1844-1854. PMID: 12609886, PMCID: PMC1302753, DOI: 10.1016/s0006-3495(03)74992-3.Peer-Reviewed Original Research