2016
Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells
Kraehling JR, Chidlow JH, Rajagopal C, Sugiyama MG, Fowler JW, Lee MY, Zhang X, RamÃrez CM, Park EJ, Tao B, Chen K, Kuruvilla L, Larriveé B, Folta-Stogniew E, Ola R, Rotllan N, Zhou W, Nagle MW, Herz J, Williams KJ, Eichmann A, Lee WL, Fernández-Hernando C, Sessa WC. Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells. Nature Communications 2016, 7: 13516. PMID: 27869117, PMCID: PMC5121336, DOI: 10.1038/ncomms13516.Peer-Reviewed Original ResearchConceptsActivin-like kinase 1Wide RNAi screenGWA data setsLDL uptakeEndothelial cellsIdentification of pathwaysLDL receptorRNAi screenEndocytic pathwayLysosomal degradationKinase 1LDL transcytosisPhysiological roleGenetic ablationUptake of LDLPathwayFunctional LDL receptorsCellsLow affinityTranscytosisUptakeALK1Unique opportunityAortic endotheliumAnimals
2014
Myeloid-Specific Disruption of Tyrosine Phosphatase Shp2 Promotes Alternative Activation of Macrophages and Predisposes Mice to Pulmonary Fibrosis
Tao B, Jin W, Xu J, Liang Z, Yao J, Zhang Y, Wang K, Cheng H, Zhang X, Ke Y. Myeloid-Specific Disruption of Tyrosine Phosphatase Shp2 Promotes Alternative Activation of Macrophages and Predisposes Mice to Pulmonary Fibrosis. The Journal Of Immunology 2014, 193: 2801-2811. PMID: 25127857, DOI: 10.4049/jimmunol.1303463.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArginaseBleomycinBronchoalveolar Lavage FluidCells, CulturedChitinInflammationInterleukin-13Interleukin-4Interleukin-5Janus Kinase 1LungMacrophage ActivationMacrophages, AlveolarMiceMice, KnockoutProtein Tyrosine Phosphatase, Non-Receptor Type 11Pulmonary FibrosisReceptors, Cell SurfaceRNA InterferenceRNA, Small InterferingSchistosoma japonicumSchistosomiasis japonicaSignal TransductionSTAT6 Transcription FactorConceptsPulmonary fibrosisIL-4RAM2 polarizationAlternative activation of macrophagesBleomycin-induced inflammationActivation of M2 macrophagesM2 polarization in vitroActivation of macrophagesPolarization in vitroTyrosine phosphatase SHP2Association of Jak1Loss of Shp2Enhanced M2 polarizationM2 macrophagesMacrophage skewingAlveolar macrophagesCre recombinaseFibrosisPhosphatase SHP2M2 phenotypeM2 skewingMacrophagesSHP2Predisposed mice
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