2023
An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells
Zhou W, Li W, Wang S, Salovska B, Hu Z, Tao B, Di Y, Punyamurtula U, Turk B, Sessa W, Liu Y. An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells. Nature Communications 2023, 14: 3803. PMID: 37365174, PMCID: PMC10293293, DOI: 10.1038/s41467-023-39514-1.Peer-Reviewed Original ResearchConceptsPhosphorylation sitesSerine/threonine kinase AktMass spectrometry-based phosphoproteomicsThreonine kinase AktAkt-dependent phosphorylationAberrant Akt activationEndothelial cellsKinase substrateKinase AktCell signalingPhosphorylation profilePhenotypic outcomesDownstream signalingAkt activationAkt1 phosphorylationHuman diseasesSystem-level analysisAKT1Vascular endothelial cellsRich resourcePhosphorylationSignalingGrowth factorAktCells
2020
BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells
Tao B, Kraehling JR, Ghaffari S, Ramirez C, Lee S, Fowler JW, Lee WL, Fernandez-Hernando C, Eichmann A, Sessa WC. BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells. Journal Of Biological Chemistry 2020, 295: 18179-18188. PMID: 33097593, PMCID: PMC7939458, DOI: 10.1074/jbc.ra120.015680.Peer-Reviewed Original ResearchConceptsBone morphogenetic protein 9Dynamin 2Caveolin-1LDL transcytosisGene expressionActivin-like kinase 1Endothelial cellsEndothelial cell quiescenceCell surface levelsSmad1/5 phosphorylationExtensive endocytosisUnbiased screenProtein 9Cell quiescenceKinase 1ALK-1BMP-9 levelsEssential roleEndocytosisEndothelial homeostasisHeavy chainSignalingInternalizationTranscytosisCells
2017
Macrophage-Restricted Shp2 Tyrosine Phosphatase Acts as a Rheostat for MMP12 through TGF-β Activation in the Prevention of Age-Related Emphysema in Mice
Xu J, Tao B, Guo X, Zhou S, Li Y, Zhang Y, Zhou Z, Cheng H, Zhang X, Ke Y. Macrophage-Restricted Shp2 Tyrosine Phosphatase Acts as a Rheostat for MMP12 through TGF-β Activation in the Prevention of Age-Related Emphysema in Mice. The Journal Of Immunology 2017, 199: 2323-2332. PMID: 28814604, DOI: 10.4049/jimmunol.1601696.Peer-Reviewed Original ResearchConceptsMatrix metalloproteinasesActivation of alveolar macrophagesLevels of matrix metalloproteinasesElevated levels of matrix metalloproteinasesChronic lung disordersFunction of Shp2Activation of macrophagesProtein tyrosine phosphatase 2Development of emphysemaLong-term observation periodAttenuated Smad2/3 phosphorylationElevation of matrix metalloproteinasesProduction of matrix metalloproteinasesLoss of Shp2Tyrosine phosphatase 2MMP12 expressionPotential etiologiesAged miceLung disordersDestruction of alveolar wallsPulmonary emphysemaSmad2/3 phosphorylationAlveolar macrophagesAlveolar morphologyExtracellular matrix degradation
2016
Cellular Phenotypic Analysis of Macrophage Activation Unveils Kinetic Responses of Agents Targeting Phosphorylation
Cao Q, Yao J, Li H, Tao B, Cai Y, Xiao P, Cheng H, Ke Y. Cellular Phenotypic Analysis of Macrophage Activation Unveils Kinetic Responses of Agents Targeting Phosphorylation. SLAS DISCOVERY 2016, 22: 51-57. PMID: 27554457, DOI: 10.1177/1087057116663166.Peer-Reviewed Original ResearchConceptsEndpoint assayHigh-throughput mannerRegulation of macrophage activationImpedance-based assayHost immune systemCell response profilesMicrobial productsPathogen clearanceGlobal screeningPhysiologically relevant dataMacrophage functionCellular impedanceMacrophage activationAssayPreventing autoimmune diseasesImmune systemKinase/phosphatasePlastic cellsPathogensMacrophagesAutoimmune diseasesReal-timeHostReal time
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