2025
Structural basis of promiscuous inhibition of Listeria virulence activator PrfA by oligopeptides
Hainzl T, Scortti M, Lindgren C, Grundström C, Krypotou E, Vázquez-Boland J, Sauer-Eriksson A. Structural basis of promiscuous inhibition of Listeria virulence activator PrfA by oligopeptides. Cell Reports 2025, 44: 115290. PMID: 39970044, DOI: 10.1016/j.celrep.2025.115290.Peer-Reviewed Original ResearchConceptsDNA-binding helix-turn-helix motifInhibit virulence gene expressionVirulence gene expressionPathogen Listeria monocytogenesPrfA activityVirulence factorsPrfAMaster regulatorsHydrophobic residuesInhibitory bindingGene expressionStructural basisBinding promiscuityPeptide bindingBinding sitesConformational changesPeptide residuesOligopeptidesPeptideInhibitory peptidesBindingPeptide backboneResiduesPromiscuous inhibitionExpression
2023
Bacteria require phase separation for fitness in the mammalian gut
Krypotou E, Townsend G, Gao X, Tachiyama S, Liu J, Pokorzynski N, Goodman A, Groisman E. Bacteria require phase separation for fitness in the mammalian gut. Science 2023, 379: 1149-1156. PMID: 36927025, PMCID: PMC10148683, DOI: 10.1126/science.abn7229.Peer-Reviewed Original ResearchConceptsMammalian gutTranscription termination factor RhoTermination factor RhoGene regulationTranscription terminationMechanisms bacteriaBacteria interactionsHuman commensalValuable targetBacteriaRhoGut microbiotaFitnessNovel clinical applicationsTherapeutic manipulationGutHuman healthCommensalRegulation
2019
Control of Bacterial Virulence through the Peptide Signature of the Habitat
Krypotou E, Scortti M, Grundström C, Oelker M, Luisi B, Sauer-Eriksson A, Vázquez-Boland J. Control of Bacterial Virulence through the Peptide Signature of the Habitat. Cell Reports 2019, 26: 1815-1827.e5. PMID: 30759392, PMCID: PMC6389498, DOI: 10.1016/j.celrep.2019.01.073.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsEcosystemGene Expression Regulation, BacterialHumansListeria monocytogenesMutationPeptidesVirulenceConceptsBacterial virulenceControl of bacterial virulenceFacultative intracellular bacterium Listeria monocytogenesOpp transport systemIntracellular bacterium Listeria monocytogenesBacterium Listeria monocytogenesGSH-binding siteVirulence programCo-crystallization studiesNutritional peptidesPrfAGenetic screeningTranscriptional activityOrganic N sourcesVirulenceNon-cysteine-containing peptidesSteric blockadeHost entryRegulation mechanismBinding sitesPeptide signaturesActive peptidesPeptideN sourcesOligopeptides
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