2012
Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport
Du Z, Wan L, Yan Q, Weinbaum S, Weinstein AM, Wang T. Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f1507-f1516. PMID: 22952281, PMCID: PMC3532483, DOI: 10.1152/ajprenal.00277.2012.Peer-Reviewed Original ResearchAngiotensin IIAngiotensin II Type 1 Receptor BlockersAnimalsBicarbonatesBiological TransportEnzyme InhibitorsFemaleHemostasisIn Vitro TechniquesKidney GlomerulusKidney TubulesLosartanMacrolidesMiceMice, KnockoutModels, AnimalProton-Translocating ATPasesReceptor, Angiotensin, Type 1SodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersRegulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport
Du Z, Yan Q, Wan L, Weinbaum S, Weinstein AM, Wang T. Regulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f386-f395. PMID: 22552936, PMCID: PMC3433864, DOI: 10.1152/ajprenal.00531.2011.Peer-Reviewed Original ResearchMeSH Keywords8-Bromo Cyclic Adenosine MonophosphateAlgorithmsAnimalsBenzazepinesBicarbonatesBiological Transport, ActiveChloridesCyclic AMP-Dependent Protein KinasesDopamineDopamine AntagonistsDopamine D2 Receptor AntagonistsFemaleIsoquinolinesKidney GlomerulusKidney TubulesKidney Tubules, ProximalMacrolidesMiceMicrovilliProton-Translocating ATPasesReceptors, Dopamine D1SodiumSulfonamidesSulpirideConceptsNa/H exchanger 3Impact of dopamineMouse proximal tubulesLuminal flowGlomerulotubular balanceCl reabsorptionProximal tubulesNa-K-ATPase activityProximal tubule reabsorptionPKA-mediated mechanismLuminal flow rateLuminal dopamineDA1 receptorsTubule reabsorptionPKA inhibitor H89Exogenous dopamineH-ATPase activityReabsorptive fluxReabsorptionDopamineNHE3 activityInhibitor H89Perfusion conditionsCoordinated modulationFlow stimulation
2010
Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells
Marin EP, Moeckel G, Al-Lamki R, Bradley J, Yan Q, Wang T, Wright PL, Yu J, Sessa WC. Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells. American Journal Of Pathology 2010, 177: 2765-2773. PMID: 20971739, PMCID: PMC2993268, DOI: 10.2353/ajpath.2010.100199.Peer-Reviewed Original ResearchConceptsUnilateral ureteral obstructionAcute tubular necrosisEpithelial cellsRenal tubular epithelial cellsMurine kidneyIschemia/reperfusionMeasurement of fibrosisDistal nephron segmentsRecruitment of macrophagesWild-type miceInflammatory gene expressionTubular epithelial cellsDe novo expressionHuman biopsy specimensRenal injuryTubular necrosisUreteral obstructionWT miceVascular injuryHistological damageBiopsy specimensCortical tubulesDeficient miceMacrophage recruitmentTissue injury
2006
Use of transgenic mice in acid-base balance studies.
Cantone A, Wang T, Pica A, Simeoni M, Capasso G. Use of transgenic mice in acid-base balance studies. Journal Of Nephrology 2006, 19 Suppl 9: s121-7. PMID: 16736435.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAcid-Base ImbalanceAnimalsCation Transport ProteinsDisease Models, AnimalDNAGene ExpressionKidney TubulesMembrane ProteinsMiceMice, TransgenicNitric Oxide SynthaseSodium-Bicarbonate SymportersSodium-Hydrogen Exchanger 1Sodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsAcid-base statusNitric oxide synthaseTransgenic miceMedullary thick ascending limbProximal tubule transportThick ascending limbSubunit expression levelsPotassium-chloride cotransporterKnockout animal modelsAcid-base balanceKidney regulationTransepithelial HCO3- absorptionNatriuretic responseBlood pressureRenal diseaseOxide synthaseDistal nephronAnimal modelsEndothelial isoformTubule transportBasolateral NHE1Proximal tubulesFunction of pendrinAscending limbHCO3- absorption
2004
Mechanical stimuli induce cleavage and nuclear translocation of the polycystin-1 C terminus
Chauvet V, Tian X, Husson H, Grimm DH, Wang T, Hieseberger T, Igarashi P, Bennett AM, Ibraghimov-Beskrovnaya O, Somlo S, Caplan MJ. Mechanical stimuli induce cleavage and nuclear translocation of the polycystin-1 C terminus. Journal Of Clinical Investigation 2004, 114: 1433-1443. PMID: 15545994, PMCID: PMC525739, DOI: 10.1172/jci21753.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell LineCell NucleusChlorocebus aethiopsCHO CellsCOS CellsCricetinaeCricetulusDogsEmbryo, MammalianEpithelial CellsKidney TubulesMembrane ProteinsMiceMice, TransgenicPolycystic Kidney, Autosomal DominantProteinsSequence DeletionSignal TransductionStress, MechanicalTranscription Factor AP-1TRPP Cation ChannelsConceptsC-terminal tailAutosomal dominant polycystic kidney diseaseCell-matrix interactionsCiliary signalingSecond genePolycystin-2Polycystin-1C-terminusNovel pathwayProteolytic cleavageNuclear translocationMechanical stimuliGenesDominant polycystic kidney diseasePolycystic kidney diseasePrecise mechanismCleavageTerminusSignalingTranslocationNucleusPathwayMechanosensory function of microvilli of the kidney proximal tubule
Du Z, Duan Y, Yan Q, Weinstein AM, Weinbaum S, Wang T. Mechanosensory function of microvilli of the kidney proximal tubule. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 13068-13073. PMID: 15319475, PMCID: PMC516518, DOI: 10.1073/pnas.0405179101.Peer-Reviewed Original Research
2003
Distribution and regulation of expression of serum‐ and glucocorticoid‐induced kinase‐1 in the rat kidney
de la Rosa D, Coric T, Todorovic N, Shao D, Wang T, Canessa C. Distribution and regulation of expression of serum‐ and glucocorticoid‐induced kinase‐1 in the rat kidney. The Journal Of Physiology 2003, 551: 455-466. PMID: 12816971, PMCID: PMC2343216, DOI: 10.1113/jphysiol.2003.042903.Peer-Reviewed Original ResearchMeSH KeywordsAdrenalectomyAldosteroneAnimalsAntibodiesAntibody SpecificityBlotting, NorthernBlotting, WesternCells, CulturedDNA, ComplementaryElectrophoresis, Polyacrylamide GelEpithelial CellsGene Expression Regulation, EnzymologicGlucocorticoidsImmediate-Early ProteinsImmunoblottingIn Vitro TechniquesIsoenzymesKidneyKidney TubulesMicroscopy, FluorescenceNuclear ProteinsProtein Serine-Threonine KinasesRatsRats, Sprague-DawleyRNASubcellular FractionsTransfectionConceptsGlucocorticoid-induced kinase 1Kinase 1Ion channelsRegulation of expressionConstitutive high expressionBasolateral membraneRenal epithelial cellsSubcellular localizationLevel of expressionRegulation of levelsEpithelial ion channelsSGK1 proteinMammalian kidneyApical membraneDirect interactionSGK1Epithelial cellsWestern blottingHigh expressionExpressionExpression of SGK1ProteinRat kidneyTransportersPhysiological changes
1998
Tubule Function in Transgenic Mice
Wang T, Giebisch G. Tubule Function in Transgenic Mice. Experimental Nephrology 1998, 6: 447-453. PMID: 9730661, DOI: 10.1159/000020554.Peer-Reviewed Original ResearchConceptsRenal tubule transportRenal electrolyte transportStrains of miceExchanger isoforms NHE2Renal transport functionsK-ATPase expressionMouse modelTubule functionTransgenic knockoutTubule transportTransgenic miceExchanger isoforms NHE1MiceTubule segmentsSelective alterationsOverexpression modelElectrolyte transportTransport functionKnockout modelsLesionsKidneyIsoforms NHE1
1995
Effects of a novel KATP channel blocker on renal tubule function and K channel activity.
Wang T, Wang W, Klein-Robbenhaar G, Giebisch G. Effects of a novel KATP channel blocker on renal tubule function and K channel activity. Journal Of Pharmacology And Experimental Therapeutics 1995, 273: 1382-9. PMID: 7791111.Peer-Reviewed Original ResearchConceptsKATP channel blockerLoop of HenleCortical collecting tubuleThick ascending limbPotassium secretionChannel blockersCollecting tubuleAscending limbPotassium recyclingRenal tubule functionK channel activitySodium reabsorptionTubule functionCl reabsorptionK secretionPotassium conductanceApical potassium conductancePrincipal cellsSecretionChannel activityBlockersHenleReabsorptionLimbRecent studies
1989
Neural control of distal tubular bicarbonate and fluid transport
Wang T, Chan Y. Neural control of distal tubular bicarbonate and fluid transport. American Journal Of Physiology 1989, 257: f72-f76. PMID: 2750926, DOI: 10.1152/ajprenal.1989.257.1.f72.Peer-Reviewed Original ResearchConceptsDistal tubulesRenal nerve activityProximal tubular reabsorptionEarly distal tubuleEffects of denervationLate distal tubuleDenervated kidneysReabsorb bicarbonateSham denervationNerve activityElectrolyte excretionTubular reabsorptionNeural regulationDenervationFluid reabsorptionBicarbonate absorptionFluid absorptionFluid samplesNeural controlVolume markerMicroperfusion methodTubulesBicarbonate concentrationJHCO3Reabsorption