2023
High dietary K+ intake inhibits proximal tubule transport
Wang T, Liu T, Xu S, Frindt G, Weinstein A, Palmer L. High dietary K+ intake inhibits proximal tubule transport. American Journal Of Physiology. Renal Physiology 2023, 325: f224-f234. PMID: 37318989, PMCID: PMC10396284, DOI: 10.1152/ajprenal.00013.2023.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFree-flow micropunctureFiltration rateUrine volumeFractional NaNephron segmentsOverall kidney functionProximal tubule transportProximal tubule functionNHE3 protein expressionExchanger isoform 3HK animalsKCl dietFraction excretionKidney functionHigh dietaryTubule functionKidney microsomesTubule transportAnesthetized animalsProximal tubulesVolume reabsorptionExcretionWestern blotControl dietHigh Dietary K Intake Inhibits Proximal Tubule Transport and Reduces GFR
Wang T, Liu T, Xu S, Frindt G, Palmer L, Weinstein A. High Dietary K Intake Inhibits Proximal Tubule Transport and Reduces GFR. Physiology 2023, 38: 5731363. DOI: 10.1152/physiol.2023.38.s1.5731363.Peer-Reviewed Original ResearchGlomerular filtration rateTF/PProximal tubulesUrine volumeTubular fluidDistal NaFractional NaReduction of GFRNephron segmentsFree-flow micropunctureOverall kidney functionDistal nephron segmentsProximal nephron segmentsKidney functionFiltration ratePt-NaFiltered loadLate DCTProximal adjustmentsModulation of NaTubule transportFractional deliveryFluid absorptionPT transportRats
2022
Regulation of glomerulotubular balance. IV. Implication of aquaporin 1 in flow-dependent proximal tubule transport and cell volume
Du Z, Yan Q, Shen E, Weinstein A, Wang T. Regulation of glomerulotubular balance. IV. Implication of aquaporin 1 in flow-dependent proximal tubule transport and cell volume. American Journal Of Physiology. Renal Physiology 2022, 323: f642-f653. PMID: 36108052, PMCID: PMC9705020, DOI: 10.1152/ajprenal.00167.2022.Peer-Reviewed Original ResearchConceptsKO miceProximal tubulesMouse proximal tubulesWater reabsorptionAcid-base parametersKnockout miceAquaporin 1 knockout miceAquaporin-1Rat kidneyRenal clearance experimentsProximal tubule transportWater channel aquaporin-1Significant differencesHigh urine flowRat proximal tubuleKidney proximal tubulesWT miceUrine flowClearance experimentsGlomerulotubular balanceBlood pHTubule transportAQP1 knockout miceMiceFluid absorption
2021
Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice
Xu S, Li J, Yang L, Wang CJ, Liu T, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice. Pflügers Archiv - European Journal Of Physiology 2021, 473: 1749-1760. PMID: 34455480, PMCID: PMC8528772, DOI: 10.1007/s00424-021-02611-5.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFemale miceUrine volumeNaCl cotransporterLow K intakeMeasurement of functionSex differencesNatriuretic responseFractional excretionK intakeTransport protein expressionFiltration rateNCC abundanceNCC expressionRenal clearanceDistal nephronLK dietCation excretionPlasma KNCC proteinNKCC2 expressionWestern blottingProtein expressionMiceFENaRestoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease
Du Z, Tian X, Ma M, Somlo S, Weinstein AM, Wang T. Restoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease. JCI Insight 2021, 6: e146041. PMID: 33886508, PMCID: PMC8262298, DOI: 10.1172/jci.insight.146041.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateGlomerulotubular balanceRenal cyst formationCyst formationReceptor 1 antagonistPolycystic kidney diseaseKidney weightUntreated miceDA1 antagonistControl miceKidney diseaseFiltration rateFractional reabsorptionCystic indexMouse modelCyst growthConditional KOHCO3- absorptionHeterozygous miceSame antagonistsMicePT transportAntagonistEpithelial ciliaHCO3- transportDeletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse
Thomson R, Dynia DW, Burlein S, Thomson BR, Booth C, Knauf F, Wang T, Aronson P. Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse. American Journal Of Physiology. Renal Physiology 2021, 320: f1106-f1122. PMID: 33938239, PMCID: PMC8285649, DOI: 10.1152/ajprenal.00556.2020.Peer-Reviewed Original ResearchConceptsKsp-cadherinCell adhesion moleculeAtypical memberKidney developmentMammalian kidneyAdult mammalian kidneyBasolateral membraneNormal kidney developmentEpithelial cellsAdhesion moleculesMutant animalsExpression analysisSpecific expressionE-cadherin expressionWestern blot analysisEpithelial phenotypePrincipal proteinE-cadherinBlot analysisMouse linesAquaporin-2CadherinCritical roleDevelopmental delayKnockout mice
2020
Replicated risk CACNA1C variants for major psychiatric disorders may serve as potential therapeutic targets for the shared depressive endophenotype.
Guo X, Fu Y, Zhang Y, Wang T, Lu L, Luo X, Wang K, Huang J, Xie T, Zheng C, Yang K, Tong J, Zuo L, Kang L, Tan Y, Jiang K, Li CR, Luo X. Replicated risk CACNA1C variants for major psychiatric disorders may serve as potential therapeutic targets for the shared depressive endophenotype. 2020, 4 PMID: 34046650, PMCID: PMC8153461.Peer-Reviewed Original ResearchMajor psychiatric disordersGenome-wide association studiesL-type voltage-gated calcium channelsMajor depressive disorderMRNA expression regulationPsychiatric disordersTherapeutic targetBipolar disorderChronic mild stress (CMS) ratsHuman hippocampusExpression regulationDepressive endophenotypeType voltage-gated calcium channelsRole of venlafaxineVoltage-gated calcium channelsPotential therapeutic targetRisk genesSignificant mRNA expressionVenlafaxine treatmentDepressive disorderStress ratsDepressive symptomsIndependent cohortCalcium channelsMRNA expressionRole of potassium channels in female reproductive system
Kim JM, Song KS, Xu B, Wang T. Role of potassium channels in female reproductive system. Obstetrics & Gynecology Science 2020, 63: 565-576. PMID: 32838485, PMCID: PMC7494774, DOI: 10.5468/ogs.20064.Peer-Reviewed Original ResearchFemale reproductive systemPotassium channelsReproductive systemGonadotropin-releasing hormone (GnRH) neuronsEndothelium-derived hyperpolarizing factorsSulfonylurea receptorSmall conductance KCaStrong myometrial contractionsSodium-activated potassium channelsProduction of progesteroneKir6.1/SUR2BKir6.2/SUR1Variety of organsLuteal granulosa cellsHormone neuronsOvarian axisResistance arteriesUterine quiescenceHyperpolarizing factorsMyometrial contractionsLate pregnancyHormone releaseCardiovascular organsGranulosa cellsChannel activatorSex Difference In Kidney Electrolyte Transport III: Impact of Low K intake on Thiazide‐Sensitive Cation Excretion in Male and Female Mice
Xu S, Li J, Yang L, Wang C, Liu T, Weinstein A, Palmer L, Wang T. Sex Difference In Kidney Electrolyte Transport III: Impact of Low K intake on Thiazide‐Sensitive Cation Excretion in Male and Female Mice. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.04495.Peer-Reviewed Original ResearchGlomerular filtration rateFemale miceUrine volumeCation excretionNCC proteinNCC activityDietary potassium intakeLow K intakeRole of NCCMeasurement of functionSex differencesNatriuretic responsePotassium intakeK intakeFiltration rateMale miceNCC abundanceNCC expressionDistal tubulesDistal nephronElectrolyte homeostasisLK dietSodium absorptionHCTZK restriction
2019
Associations between dietary patterns and 10-year cardiovascular disease risk score levels among Chinese coal miners——a cross-sectional study
Sun Q, Ma J, Wang H, Xu S, Zhao J, Gao Q, Huang J, Wang T. Associations between dietary patterns and 10-year cardiovascular disease risk score levels among Chinese coal miners——a cross-sectional study. BMC Public Health 2019, 19: 1704. PMID: 31856787, PMCID: PMC6923962, DOI: 10.1186/s12889-019-8070-9.Peer-Reviewed Original ResearchConceptsRisk score levelDietary patternsCardiovascular diseaseValid semi-quantitative food frequency questionnaireSemi-quantitative food frequency questionnaireEffective dietary guidelinesFood frequency questionnaireHigh-risk populationCross-sectional studyLarge health burdenCoal minersFrequency questionnaireDietary intakeHealth burdenDietary guidelinesMethodsThe studyLogistic regressionConclusionsThis studySocioeconomic factorsScore levelDiseaseDietary structureAssociationChinese coal minersGround workersSex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice
Li J, Xu S, Yang L, Yang J, Wang CJ, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice. American Journal Of Physiology. Renal Physiology 2019, 317: f967-f977. PMID: 31390232, PMCID: PMC6843050, DOI: 10.1152/ajprenal.00125.2019.Peer-Reviewed Original ResearchConceptsHK dietCation excretionWT animalsFemale animalsMale animalsSex differencesRenal clearance experimentsGlomerular filtration rateR KO miceExchanger isoform 3Distal NaHK intakeWestern blot analysisFractional excretionUrine volumeFiltration rateFemale miceKO miceNHE3 abundanceClearance experimentsNCC abundanceLittle sex differenceKnockout miceENaC expressionExcretionThe bidirectional causal relationships of insomnia with five major psychiatric disorders: A Mendelian randomization study
Gao X, Meng L, Ma K, Liang J, Wang H, Gao Q, Wang T. The bidirectional causal relationships of insomnia with five major psychiatric disorders: A Mendelian randomization study. European Psychiatry 2019, 60: 79-85. PMID: 31234011, DOI: 10.1016/j.eurpsy.2019.05.004.Peer-Reviewed Original ResearchConceptsMajor psychiatric disordersPsychiatric disordersBipolar disorderAssociation of insomniaMendelian randomization studyAutism spectrum disorderOdds ratioObservational studySpectrum disorderInsomniaRandomization studyEffective interventionsCausal relationshipEgger regressionDisordersDisease modelsCausal roleMendelian randomizationConvincing evidenceGenome-wide association studiesAssociationAetiology of bipolar disorder: contribution of the L-type voltage-gated calcium channels
Guo X, Liu D, Wang T, Luo X. Aetiology of bipolar disorder: contribution of the L-type voltage-gated calcium channels. General Psychiatry 2019, 32: e100009. PMID: 31179430, PMCID: PMC6551429, DOI: 10.1136/gpsych-2018-100009.Peer-Reviewed Original Research
2018
Potassium conservation is impaired in mice with reduced renal expression of Kir4.1
Malik S, Lambert E, Zhang J, Wang T, Clark H, Cypress M, Goldman B, Porter GA, Pena S, Nino W, Gray D. Potassium conservation is impaired in mice with reduced renal expression of Kir4.1. American Journal Of Physiology. Renal Physiology 2018, 315: f1271-f1282. PMID: 30110571, PMCID: PMC6293297, DOI: 10.1152/ajprenal.00022.2018.Peer-Reviewed Original ResearchMeSH KeywordsAlkalosisAnimalsAquaporin 3Gene Knockdown TechniquesGenotypeHypercalcemiaHyperkalemiaHypernatremiaKidney Concentrating AbilityMice, Inbred C57BLMice, KnockoutNephronsPhenotypePhosphorylationPotassium Channels, Inwardly RectifyingPotassium, DietaryRenal ReabsorptionSolute Carrier Family 12, Member 3ConceptsMg-free dietSevere urinary concentrating defectReduced renal expressionRenal K wastingSeSAME/EAST syndromeDistal convoluted tubuleKir4.1 protein expressionWild-type miceUrinary concentrating defectCre-LoxP methodologyAquaporin 3 expressionRelative hypercalcemiaRenal expressionPotassium conservationMetabolic alkalosisNCC expressionChannel Kir4.1Distal nephronKnockout miceConvoluted tubulesCotransporter expressionEAST syndromeHypokalemiaMiceReduced expressionRegulation of renal Na transporters in response to dietary K
Yang L, Xu S, Guo X, Uchida S, Weinstein AM, Wang T, Palmer LG. Regulation of renal Na transporters in response to dietary K. American Journal Of Physiology. Renal Physiology 2018, 315: f1032-f1041. PMID: 29923764, PMCID: PMC6230734, DOI: 10.1152/ajprenal.00117.2018.Peer-Reviewed Original Research
2017
Urinary bladder hypertrophy characteristic of male ROMK Bartter’s mice does not occur in female mice
Kim JM, Xu S, Guo X, Hu H, Dong K, Wang T. Urinary bladder hypertrophy characteristic of male ROMK Bartter’s mice does not occur in female mice. AJP Regulatory Integrative And Comparative Physiology 2017, 314: r334-r341. PMID: 29092859, PMCID: PMC5899254, DOI: 10.1152/ajpregu.00315.2017.Peer-Reviewed Original ResearchConceptsKO miceBladder hypertrophyBladder weightUrinary bladder hypertrophyRenal outer medullary potassium channelSeverity of hydronephrosisWild-type miceROMK knockout miceBladder capacityDetrusor muscleWT miceUrinary tractBartter's syndromeFemale miceSalt wastingHydronephrosisKnockout miceROMK expressionMiceBladderHypertrophyPotassium channelsMRNA levelsSignificant enlargementSyndromeGender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice
Li J, Hatano R, Xu S, Wan L, Yang L, Weinstein AM, Palmer L, Wang T. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice. American Journal Of Physiology. Renal Physiology 2017, 313: f505-f513. PMID: 28566500, PMCID: PMC5582908, DOI: 10.1152/ajprenal.00087.2017.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsDiuresisFemaleHydrochlorothiazideKidneyMaleMice, KnockoutNatriuresisPhenotypeProtein Serine-Threonine KinasesReceptor, Angiotensin, Type 1Receptors, DrugSex CharacteristicsSodium Chloride SymportersSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSolute Carrier Family 12, Member 3ConceptsUrine volumeKO miceCotransporter activityRenal clearance experimentsReceptor knockout miceGlomerular filtration rateBolus intravenous injectionDelivery of NaClNCC protein expressionGender differencesFiltration rateFemale miceAT1A receptorClearance experimentsKO animalsIntravenous injectionNCC expressionDistal nephronFractional NaKnockout miceMale KOProximal tubulesHCTZMiceNHE3 expressionRegulation of glomerulotubular balance: flow-activated proximal tubule function
Wang T, Weinbaum S, Weinstein AM. Regulation of glomerulotubular balance: flow-activated proximal tubule function. Pflügers Archiv - European Journal Of Physiology 2017, 469: 643-654. PMID: 28271233, PMCID: PMC6162058, DOI: 10.1007/s00424-017-1960-8.Peer-Reviewed Original Research
2016
Kidney Tubular Ablation of Ocrl/Inpp5b Phenocopies Lowe Syndrome Tubulopathy
Inoue K, Balkin DM, Liu L, Nandez R, Wu Y, Tian X, Wang T, Nussbaum R, De Camilli P, Ishibe S. Kidney Tubular Ablation of Ocrl/Inpp5b Phenocopies Lowe Syndrome Tubulopathy. Journal Of The American Society Of Nephrology 2016, 28: 1399-1407. PMID: 27895154, PMCID: PMC5407733, DOI: 10.1681/asn.2016080913.Peer-Reviewed Original ResearchConceptsEarly embryonic lethalityTransporter 5Dent's diseaseIndependent endocytosisEmbryonic lethalityRedundant functionsType 2 inositolHuman phenotypesProximal tubule endocytosisOculocerebrorenal syndromeGenetic ablationCellular levelGermline knockoutLowe syndromeEndocytosisMouse backgroundMice resultsMutationsInositolLow molecular weight proteinuriaINPP5BParalogsProximal tubule functionDramatic effectOCRLPotassium Channelopathies and Gastrointestinal Ulceration
Han J, Lee SH, Giebisch G, Wang T. Potassium Channelopathies and Gastrointestinal Ulceration. Gut And Liver 2016, 10: 881-889. PMID: 27784845, PMCID: PMC5087926, DOI: 10.5009/gnl15414.Peer-Reviewed Original ResearchConceptsPotassium channelsGI tractPotassium channelopathiesAdenosine triphosphate-sensitive potassium channel openerTriphosphate-sensitive potassium channel openerNonsteroidal anti-inflammatory drugsAntianginal drug nicorandilAnti-inflammatory drugsPotassium channel openersOccurrence of ulcerationPotassium channel activityLong-term useRegulation of secretionGastrointestinal ulcerationUlcerative colitisPeptic ulcerationChannel openersGastric acidGastrointestinal tractUlcerationDifferent biological actionsTransporter inhibitorsAnal regionPotassium homeostasisTract