2018
Regulation of renal Na transporters in response to dietary K
Yang L, Xu S, Guo X, Uchida S, Weinstein AM, Wang T, Palmer LG. Regulation of renal Na transporters in response to dietary K. American Journal Of Physiology. Renal Physiology 2018, 315: f1032-f1041. PMID: 29923764, PMCID: PMC6230734, DOI: 10.1152/ajprenal.00117.2018.Peer-Reviewed Original Research
2017
Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice
Li J, Hatano R, Xu S, Wan L, Yang L, Weinstein AM, Palmer L, Wang T. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice. American Journal Of Physiology. Renal Physiology 2017, 313: f505-f513. PMID: 28566500, PMCID: PMC5582908, DOI: 10.1152/ajprenal.00087.2017.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsDiuresisFemaleHydrochlorothiazideKidneyMaleMice, KnockoutNatriuresisPhenotypeProtein Serine-Threonine KinasesReceptor, Angiotensin, Type 1Receptors, DrugSex CharacteristicsSodium Chloride SymportersSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSolute Carrier Family 12, Member 3ConceptsUrine volumeKO miceCotransporter activityRenal clearance experimentsReceptor knockout miceGlomerular filtration rateBolus intravenous injectionDelivery of NaClNCC protein expressionGender differencesFiltration rateFemale miceAT1A receptorClearance experimentsKO animalsIntravenous injectionNCC expressionDistal nephronFractional NaKnockout miceMale KOProximal tubulesHCTZMiceNHE3 expression
2015
Regulation of glomerulotubular balance. III. Implication of cytosolic calcium in flow-dependent proximal tubule transport
Du Z, Weinbaum S, Weinstein AM, Wang T. Regulation of glomerulotubular balance. III. Implication of cytosolic calcium in flow-dependent proximal tubule transport. American Journal Of Physiology. Renal Physiology 2015, 308: f839-f847. PMID: 25651568, PMCID: PMC4398834, DOI: 10.1152/ajprenal.00601.2014.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBicarbonatesBiological TransportCalciumCalcium-Transporting ATPasesChelating AgentsCytosolEnzyme InhibitorsIn Vitro TechniquesInositol 1,4,5-Trisphosphate ReceptorsKidney Tubules, ProximalKineticsMice, Inbred C57BLPerfusionRenal ReabsorptionSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsNa/H exchanger 3Flow-induced increaseGlomerulotubular balanceProximal tubulesHCO3- reabsorptionCytosolic calciumAngiotensin II receptorsProximal tubule transportReceptor-mediated intracellularReabsorption of fluidMouse proximal tubulesChelator BAPTA-AMPerfusion rateH-ATPase activityControl tubulesII receptorsTubule transportBAPTA-AMInhibitor thapsigarginExchanger 3ReabsorptionBaseline flowHCO3- transportTubulesBAPTA
2013
Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion
Li H, Du Z, Barone S, Rubera I, McDonough A, Tauc M, Zahedi K, Wang T, Soleimani M. Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion. Journal Of Molecular Medicine 2013, 91: 951-963. PMID: 23508938, PMCID: PMC3730089, DOI: 10.1007/s00109-013-1015-3.Peer-Reviewed Original ResearchConceptsKO miceMetabolic acidosisBicarbonate reabsorptionAcid loadNHE3 knockout miceProximal tubule NHE3Mild metabolic acidosisCre transgenic miceExchanger NHE3NH4Cl acid loadMicroperfusion studiesWT miceKnockout miceFloxed miceTransgenic micePathophysiologic statesExcretion rateBicarbonate absorptionNH4Cl loadingMutant miceVolume reabsorptionWestern blotMiceSpecific knockoutAbsorption defect
2012
Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport
Du Z, Wan L, Yan Q, Weinbaum S, Weinstein AM, Wang T. Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f1507-f1516. PMID: 22952281, PMCID: PMC3532483, DOI: 10.1152/ajprenal.00277.2012.Peer-Reviewed Original ResearchAngiotensin IIAngiotensin II Type 1 Receptor BlockersAnimalsBicarbonatesBiological TransportEnzyme InhibitorsFemaleHemostasisIn Vitro TechniquesKidney GlomerulusKidney TubulesLosartanMacrolidesMiceMice, KnockoutModels, AnimalProton-Translocating ATPasesReceptor, Angiotensin, Type 1SodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen Exchangers
2011
Chloride Channel (Clc)-5 Is Necessary for Exocytic Trafficking of Na+/H+ Exchanger 3 (NHE3)*
Lin Z, Jin S, Duan X, Wang T, Martini S, Hulamm P, Cha B, Hubbard A, Donowitz M, Guggino SE. Chloride Channel (Clc)-5 Is Necessary for Exocytic Trafficking of Na+/H+ Exchanger 3 (NHE3)*. Journal Of Biological Chemistry 2011, 286: 22833-22845. PMID: 21561868, PMCID: PMC3123051, DOI: 10.1074/jbc.m111.224998.Peer-Reviewed Original ResearchConceptsKO miceTrafficking of NHE3Proximal tubulesOpossum kidney cellsNHE3 activityDent's diseaseClC-5Surface expressionNHE3 surface expressionKidney cellsRenal proximal tubulesTotal protein levelsChloride/proton exchangerRates of basalReduced surface expressionKnockdown cellsParathyroid hormoneWT miceDegree of inhibitionCLCN5 geneSurface NHE3MiceTubule perfusionReduced expressionTwo-photon microscopy
2010
Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells
Duan Y, Weinstein AM, Weinbaum S, Wang T. Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 21860-21865. PMID: 21106755, PMCID: PMC3003103, DOI: 10.1073/pnas.1015751107.Peer-Reviewed Original ResearchConceptsV-ATPaseFluid shear stressActin cytoskeletonNa/K-ATPaseIntact actin cytoskeletonApical plasma membraneK-ATPaseIntact microtubule networkShear stress-induced changesCellular functionsProximal tubule cellsApical NHE3Plasma membraneTransporter localizationMicrotubule networkIntracellular compartmentsWestern blot analysisCytochalasin DTubule cellsMPT cellsMicrotubule polymerizationTraffickingMouse proximal tubule cellsBlot analysisConfocal microscopy
2008
Mouse model of type II Bartter's syndrome. II. Altered expression of renal sodium- and water-transporting proteins
Wagner CA, Loffing-Cueni D, Yan Q, Schulz N, Fakitsas P, Carrel M, Wang T, Verrey F, Geibel JP, Giebisch G, Hebert SC, Loffing J. Mouse model of type II Bartter's syndrome. II. Altered expression of renal sodium- and water-transporting proteins. American Journal Of Physiology. Renal Physiology 2008, 294: f1373-f1380. PMID: 18322017, DOI: 10.1152/ajprenal.00613.2007.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAnimalsBartter SyndromeCarrier ProteinsCation Transport ProteinsDinoprostoneDisease Models, AnimalEpithelial Sodium ChannelsKidney Tubules, DistalKidney Tubules, ProximalLoop of HenleMiceMice, Mutant StrainsPotassium Channels, Inwardly RectifyingReverse Transcriptase Polymerase Chain ReactionSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSodium-Phosphate Cotransporter Proteins, Type IIaSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 1Up-RegulationWaterConceptsThick ascending limbBartter's syndromeBartter-like phenotypeType II Bartter's syndromeWild-type miceAntenatal Bartter syndromeWild-type littermatesROMK null micePlasma aldosteroneMaternal polyhydramniosRenal sodiumVolume depletionRenal tubulopathyMouse modelSemiquantitative immunoblottingProximal tubulesAscending limbKidney homogenatesSyndromeHenle's loopNull miceDCT cellsWater transport proteinsCompensatory mechanismsMice
2007
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity
Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. American Journal Of Physiology. Renal Physiology 2007, 293: f212-f218. PMID: 17409282, DOI: 10.1152/ajprenal.00042.2007.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAnimalsCells, CulturedColforsinCyclic AMP-Dependent Protein KinasesElectrophoresis, Polyacrylamide GelKidneyKidney Tubules, ProximalMaleMicrovilliParathyroid HormonePhosphodiesterase InhibitorsPhosphorylationRatsRats, Sprague-DawleySerineSodiumSodium RadioisotopesSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersStimulation, ChemicalConceptsSerine 552NHE3 phosphorylationNHE3 activityMicrovillar membrane vesiclesPhosphorylation of NHE3Direct phosphorylationPhosphospecific antibodiesSprague-Dawley ratsExchanger type 3PKA activationMembrane vesiclesSite-specific changesPhosphorylationTransport activityOpossum kidney cellsParathyroid hormoneIntravenous infusionRat modelExchange activityKidney cellsOKP cellsNHE3 inhibitionPKACell modelExact roleFlow-dependent transport in a mathematical model of rat proximal tubule
Weinstein A, Weinbaum S, Duan Y, Du Z, Yan Q, Wang T. Flow-dependent transport in a mathematical model of rat proximal tubule. American Journal Of Physiology. Renal Physiology 2007, 292: f1164-f1181. PMID: 17213461, DOI: 10.1152/ajprenal.00392.2006.Peer-Reviewed Original Research
2006
Use of transgenic mice in acid-base balance studies.
Cantone A, Wang T, Pica A, Simeoni M, Capasso G. Use of transgenic mice in acid-base balance studies. Journal Of Nephrology 2006, 19 Suppl 9: s121-7. PMID: 16736435.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAcid-Base ImbalanceAnimalsCation Transport ProteinsDisease Models, AnimalDNAGene ExpressionKidney TubulesMembrane ProteinsMiceMice, TransgenicNitric Oxide SynthaseSodium-Bicarbonate SymportersSodium-Hydrogen Exchanger 1Sodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsAcid-base statusNitric oxide synthaseTransgenic miceMedullary thick ascending limbProximal tubule transportThick ascending limbSubunit expression levelsPotassium-chloride cotransporterKnockout animal modelsAcid-base balanceKidney regulationTransepithelial HCO3- absorptionNatriuretic responseBlood pressureRenal diseaseOxide synthaseDistal nephronAnimal modelsEndothelial isoformTubule transportBasolateral NHE1Proximal tubulesFunction of pendrinAscending limbHCO3- absorption
2005
Axial flow modulates proximal tubule NHE3 and H-ATPase activities by changing microvillus bending moments
Du Z, Yan Q, Duan Y, Weinbaum S, Weinstein AM, Wang T. Axial flow modulates proximal tubule NHE3 and H-ATPase activities by changing microvillus bending moments. American Journal Of Physiology. Renal Physiology 2005, 290: f289-f296. PMID: 16144961, DOI: 10.1152/ajprenal.00255.2005.Peer-Reviewed Original ResearchRole of PDZK1 in membrane expression of renal brush border ion exchangers
Thomson RB, Wang T, Thomson BR, Tarrats L, Girardi A, Mentone S, Soleimani M, Kocher O, Aronson PS. Role of PDZK1 in membrane expression of renal brush border ion exchangers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13331-13336. PMID: 16141316, PMCID: PMC1201624, DOI: 10.1073/pnas.0506578102.Peer-Reviewed Original ResearchConceptsProximal tubulesExpression of NHE3Functional activityRole of PDZK1Protein PDZK1Reabsorption of NaBrush border expressionMammalian kidneyCFEXBrush border localizationMutant miceGST fusion proteinProtein expressionKidneyBrush border membrane proteinsExchanger NHE3Membrane expressionNHE3Brush borderBrush border membrane vesiclesPDZK1 interactionTubulesNormal expressionMembrane proteinsPDZK1
2004
NHE2‐mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice
Bailey MA, Giebisch G, Abbiati T, Aronson PS, Gawenis LR, Shull GE, Wang T. NHE2‐mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice. The Journal Of Physiology 2004, 561: 765-775. PMID: 15604231, PMCID: PMC1665379, DOI: 10.1113/jphysiol.2004.074716.Peer-Reviewed Original ResearchConceptsBicarbonate excretionDistal tubulesBicarbonate reabsorptionNHE3 null miceBicarbonate loadEarly distal tubule fluidNull miceNephron segmentsVivo micropunctureDistal tubule fluidProximal tubule bicarbonate reabsorptionRenal clearance experimentsUrinary bicarbonate excretionGlomerular filtration rateDistal nephron segmentsUrinary bicarbonate lossRat distal tubulesDistal bicarbonate reabsorptionExcretion of bicarbonateMetabolic acidosisFiltration rateVivo microperfusionClearance experimentsReabsorption capacityMicroperfusion experimentsMechanosensory function of microvilli of the kidney proximal tubule
Du Z, Duan Y, Yan Q, Weinstein AM, Weinbaum S, Wang T. Mechanosensory function of microvilli of the kidney proximal tubule. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 13068-13073. PMID: 15319475, PMCID: PMC516518, DOI: 10.1073/pnas.0405179101.Peer-Reviewed Original Research
2003
Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice
Beck F, Neuhofer W, Dörge A, Giebisch G, Wang T. Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice. Pflügers Archiv - European Journal Of Physiology 2003, 446: 100-105. PMID: 12690468, DOI: 10.1007/s00424-002-1001-z.Peer-Reviewed Original ResearchConceptsIntracellular Na concentrationNa/K-ATPaseΑ1 subunitTubule cellsProximal convoluted tubule cellsDistal convoluted tubuleWild-type miceNa/K-ATPase activityPCT cellsConvoluted tubule cellsIntracellular Cl- concentrationK-ATPaseWestern blot analysisK-ATPase activityNa concentrationRenal cortexCell Na concentrationConvoluted tubulesNa/K-ATPase α1 subunitNa/HNHE3 deficiencyK-ATPase α1-subunitMiceFluid absorption
2002
Use of transgenic animals to study renal acid-base transport.
Wang T, Giebisch G, Aronson PS. Use of transgenic animals to study renal acid-base transport. Journal Of Nephrology 2002, 15 Suppl 5: s151-60. PMID: 12027214.Commentaries, Editorials and LettersConceptsAcid-base transportSpecific transporter isoformsSuch knockout miceRenal acid-base transportRenal acid-base homeostasisTransgenic miceTransporter isoformsPump subunitsTransgenic animalsKnockout micePhysiological roleATPase isoformsTransport deficiencyMolecular levelAcid-base homeostasisIsoformsAdaptive mechanismsCarbonic anhydraseCompensatory-adaptive mechanismsUseful experimental modelTransportersRegulatory mediatorsNHE isoformsNitric oxideExperimental model
2001
Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron
Wang T, Hropot M, Aronson P, Giebisch G. Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron. American Journal Of Physiology. Renal Physiology 2001, 281: f1117-f1122. PMID: 11704563, DOI: 10.1152/ajprenal.2001.281.6.f1117.Peer-Reviewed Original ResearchConceptsLoop of HenleHOE 694Proximal tubulesAddition of ethylisopropylamilorideDistal convoluted tubuleBicarbonate reabsorptionConvoluted tubulesSitu microperfusionRat kidneySignificant inhibitionNHE isoformsHenlePredominant isoformTubulesNHE3Functional roleInhibitionNHE2Apical membraneIsoformsApical isoformKidneyMicroperfusionEthylisopropylamilorideNephronEssential role of NHE3 in facilitating formate-dependent NaCl absorption in the proximal tubule
Wang T, Yang C, Abbiati T, Shull G, Giebisch G, Aronson P. Essential role of NHE3 in facilitating formate-dependent NaCl absorption in the proximal tubule. American Journal Of Physiology. Renal Physiology 2001, 281: f288-f292. PMID: 11457720, DOI: 10.1152/ajprenal.2001.281.2.f288.Peer-Reviewed Original Research
1999
Mechanism of proximal tubule bicarbonate absorption in NHE3 null mice
Wang T, Yang C, Abbiati T, Schultheis P, Shull G, Giebisch G, Aronson P. Mechanism of proximal tubule bicarbonate absorption in NHE3 null mice. American Journal Of Physiology 1999, 277: f298-f302. PMID: 10444585, DOI: 10.1152/ajprenal.1999.277.2.f298.Peer-Reviewed Original Research