2013
Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion
Li H, Du Z, Barone S, Rubera I, McDonough A, Tauc M, Zahedi K, Wang T, Soleimani M. Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion. Journal Of Molecular Medicine 2013, 91: 951-963. PMID: 23508938, PMCID: PMC3730089, DOI: 10.1007/s00109-013-1015-3.Peer-Reviewed Original ResearchConceptsKO miceMetabolic acidosisBicarbonate reabsorptionAcid loadNHE3 knockout miceProximal tubule NHE3Mild metabolic acidosisCre transgenic miceExchanger NHE3NH4Cl acid loadMicroperfusion studiesWT miceKnockout miceFloxed miceTransgenic micePathophysiologic statesExcretion rateBicarbonate absorptionNH4Cl loadingMutant miceVolume reabsorptionWestern blotMiceSpecific knockoutAbsorption defect
2004
NHE2‐mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice
Bailey MA, Giebisch G, Abbiati T, Aronson PS, Gawenis LR, Shull GE, Wang T. NHE2‐mediated bicarbonate reabsorption in the distal tubule of NHE3 null mice. The Journal Of Physiology 2004, 561: 765-775. PMID: 15604231, PMCID: PMC1665379, DOI: 10.1113/jphysiol.2004.074716.Peer-Reviewed Original ResearchConceptsBicarbonate excretionDistal tubulesBicarbonate reabsorptionNHE3 null miceBicarbonate loadEarly distal tubule fluidNull miceNephron segmentsVivo micropunctureDistal tubule fluidProximal tubule bicarbonate reabsorptionRenal clearance experimentsUrinary bicarbonate excretionGlomerular filtration rateDistal nephron segmentsUrinary bicarbonate lossRat distal tubulesDistal bicarbonate reabsorptionExcretion of bicarbonateMetabolic acidosisFiltration rateVivo microperfusionClearance experimentsReabsorption capacityMicroperfusion experiments
2002
Role of iNOS and eNOS in modulating proximal tubule transport and acid-base balance
Wang T. Role of iNOS and eNOS in modulating proximal tubule transport and acid-base balance. American Journal Of Physiology. Renal Physiology 2002, 283: f658-f662. PMID: 12217856, DOI: 10.1152/ajprenal.00243.2001.Peer-Reviewed Original ResearchConceptsNeuronal nitric oxide synthaseInducible NOSEndothelial NOSProximal tubulesKnockout miceRole of iNOSNOS knockout miceENOS knockout miceINOS knockout miceIsoforms of NOSNitric oxide synthaseProximal tubule transportAcid-base statusAcid-base balanceINOS knockoutWild-type controlsKidney proximal tubulesMetabolic acidosisOxide synthaseTubule transportInhibitor LMiceFluid absorptionNOSTubules
2000
Defective fluid and HCO3 − absorption in proximal tubule of neuronal nitric oxide synthase-knockout mice
Wang T, Inglis F, Kalb R. Defective fluid and HCO3 − absorption in proximal tubule of neuronal nitric oxide synthase-knockout mice. American Journal Of Physiology. Renal Physiology 2000, 279: f518-f524. PMID: 10966931, DOI: 10.1152/ajprenal.2000.279.3.f518.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAnimalsBicarbonatesBiological TransportBlood Gas AnalysisBody FluidsElectrolytesEnzyme InhibitorsInjections, IntravenousKidney Tubules, ProximalMiceMice, Inbred C57BLMice, KnockoutNG-Nitroarginine Methyl EsterNitric Oxide SynthaseNitric Oxide Synthase Type IPotassiumSodiumConceptsWild-type miceNNOS knockout miceL-NAMEProximal tubulesBlood pressureMetabolic acidosisNeuronal nitric oxide synthase knockout miceNitric oxide synthase knockout miceAbsorption of fluidFluid absorptionNeuronal nitric oxide synthaseRenal clearance techniquesMean blood pressureSignificant metabolic acidosisSynthase knockout miceMonomethyl-L-arginine methyl esterNitric oxide synthaseWild-type control animalsProximal tubule transportLuminal perfusion solutionAcid-base balanceIntravenous bolusUrinary excretionUrine volumeClearance technique
1998
Effect of metabolic acidosis on NaCl transport in the proximal tubule
Wang T, Egbert A, Aronson P, Giebisch G. Effect of metabolic acidosis on NaCl transport in the proximal tubule. American Journal Of Physiology 1998, 274: f1015-f1019. PMID: 9841491, DOI: 10.1152/ajprenal.1998.274.6.f1015.Peer-Reviewed Original ResearchConceptsMetabolic acidosisProximal tubulesNaCl reabsorptionRat proximal tubule segmentsAcidotic ratsNaCl transportNaCl absorptionLate proximal tubuleProximal tubule segmentsControl ratsCapillary perfusateBicarbonate absorptionNephron segmentsAcidosisTubule segmentsRatsRecent evidenceJclTubulesFormate exchangeReabsorptionDifferential regulation