Featured Publications
ESCRT-mediated membrane repair protects tumor-derived cells against T cell attack
Ritter AT, Shtengel G, Xu CS, Weigel A, Hoffman DP, Freeman M, Iyer N, Alivodej N, Ackerman D, Voskoboinik I, Trapani J, Hess HF, Mellman I. ESCRT-mediated membrane repair protects tumor-derived cells against T cell attack. Science 2022, 376: 377-382. PMID: 35446649, DOI: 10.1126/science.abl3855.Peer-Reviewed Original ResearchConceptsESCRT machineryEndosomal Sorting ComplexCancer-derived cellsESCRT proteinsTarget cellsTumor-derived cellsPlasma membranePerforin poresTransport proteinsMembrane woundsMembrane repairRelease of perforinMachineryCytosolProteinCellsCytolytic attackCytotoxic T lymphocytesESCRTTumor cellsPerforin releaseGranzymesRepairApoptosisCell attack3D FIB-SEM reconstruction of microtubule–organelle interaction in whole primary mouse β cells
Müller A, Schmidt D, Xu CS, Pang S, D’Costa J, Kretschmar S, Münster C, Kurth T, Jug F, Weigert M, Hess HF, Solimena M. 3D FIB-SEM reconstruction of microtubule–organelle interaction in whole primary mouse β cells. Journal Of Cell Biology 2020, 220: e202010039. PMID: 33326005, PMCID: PMC7748794, DOI: 10.1083/jcb.202010039.Peer-Reviewed Original ResearchConceptsInsulin secretory granulesΒ-cellsSecretory granulesPrimary mammalian cellsFirst 3D reconstructionPrimary mouse β-cellsMouse β-cellsMammalian cellsMicrotubule organizationPlasma membraneIntracellular traffickingIslet β-cellsMicrotubule networkMicrotubulesUnprecedented resolutionCell constituentsMicrotubule numberCell functionGolgi apparatiCentriolesCellsEndocrine cellsGlucose stimulationEndomembranesGranules
2024
Periodic ER-plasma membrane junctions support long-range Ca2+ signal integration in dendrites
Benedetti L, Fan R, Weigel A, Moore A, Houlihan P, Kittisopikul M, Park G, Petruncio A, Hubbard P, Pang S, Xu C, Hess H, Saalfeld S, Rangaraju V, Clapham D, De Camilli P, Ryan T, Lippincott-Schwartz J. Periodic ER-plasma membrane junctions support long-range Ca2+ signal integration in dendrites. Cell 2024, 188: 484-500.e22. PMID: 39708809, DOI: 10.1016/j.cell.2024.11.029.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulum-plasma membrane junctionsEndoplasmic reticulum-plasma membranePlasma membrane of dendritesVoltage-gated Ca<sup>2+</sup> channelsER-plasma membrane junctionsMembrane of dendritesProtein kinase IIRyanodine receptorSynaptic inputsDendritic computationsSpine stimulationNeuronal dendritesKinase IIIntracellular signalingMembrane junctionsPlasma membraneER tubulesSignal propagationSignal transmissionSubcellular architectureRyanodineLadder-like arraysLocal activationReleaseDendritesVolume microscopic analysis of membrane contact sites in mouse kidney renal proximal tubule epithelial cells
Pandya R, Pang S, Lackner E, Reyna-Neyra A, Li W, Sy K, Burdyniuk M, Weisz O, Xu C, Caplan M. Volume microscopic analysis of membrane contact sites in mouse kidney renal proximal tubule epithelial cells. Physiology 2024, 39: 1086. DOI: 10.1152/physiol.2024.39.s1.1086.Peer-Reviewed Original ResearchMembrane contact sitesProximal tubule epithelial cellsTubule epithelial cellsEndoplasmic reticulumEpithelial cellsContact sitesPlasma membraneER volumeRenal proximal tubule epithelial cellsFunction of membrane contact sitesVolume of endoplasmic reticulumProximal tubule cellsInter-organelle communicationBasal-lateral surfacesRenal epithelial cellsAdvanced imaging techniquesMedian volumeTubule cellsMale miceCell plasma membraneRenal cortexScanning electron microscopyFIB-SEMMouse kidneySmooth ER