2025
Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM
Renganathan B, Moore A, Yeo W, Petruncio A, Ackerman D, Weigel A, Team T, Pasolli H, Xu C, Shtengel G, Hess H, Serpinskaya A, Zhang H, Lippincott-Schwartz J, Gelfand V. Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM. Journal Of Cell Biology 2025, 224: e202406054. PMID: 40062969, PMCID: PMC11893169, DOI: 10.1083/jcb.202406054.Peer-Reviewed Original Research
2022
Journal Club: Neurofilament Light Chain Levels in Anti-NMDAR Encephalitis and Primary Psychiatric Psychosis
Deerhake M, Giarraputo J, Gupta M, Eckstein C. Journal Club: Neurofilament Light Chain Levels in Anti-NMDAR Encephalitis and Primary Psychiatric Psychosis. Neurology 2022, 99: 854-857. PMID: 36038273, PMCID: PMC9651450, DOI: 10.1212/wnl.0000000000201235.Peer-Reviewed Original ResearchMeSH KeywordsAnti-N-Methyl-D-Aspartate Receptor EncephalitisHumansIntermediate FilamentsPsychotic DisordersReceptors, N-Methyl-D-AspartateConceptsNMDA receptor encephalitisAntibody testingNeurofilament light chain levelsAutoimmune brain disordersAnti-NMDAR encephalitisSubset of patientsPrimary psychiatric disordersLight chain levelsReceptor encephalitisLumbar punctureNMDA receptorsSerum testPsychiatric disordersBrain disordersClinical settingEncephalitisDisordersPatientsPuncturePsychosisCSFChain levelAntibodiesReceptors
2021
Keratin 1 as a cell-surface receptor in cancer
Ogunnigbagbe O, Bunick CG, Kaur K. Keratin 1 as a cell-surface receptor in cancer. Biochimica Et Biophysica Acta (BBA) - Reviews On Cancer 2021, 1877: 188664. PMID: 34890750, PMCID: PMC8818032, DOI: 10.1016/j.bbcan.2021.188664.Peer-Reviewed Original ResearchConceptsImportant cellular functionsGlycine-rich loopType II keratinsCell surface receptorsReceptor-mediated endocytosisKeratin 1Cell surface expressionCellular functionsC-terminusCentral domainEpithelial cell markersCancer progressionIntermediate filamentsFibrous proteinsCancer cellsK1 expressionOxidative stressCancerous cellsCell markersImmune evasionCellsExpressionKeratinCsk1Endocytosis
2020
Recent insight into intermediate filament structure
Eldirany SA, Lomakin IB, Ho M, Bunick CG. Recent insight into intermediate filament structure. Current Opinion In Cell Biology 2020, 68: 132-143. PMID: 33190098, PMCID: PMC7925366, DOI: 10.1016/j.ceb.2020.10.001.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCytoskeletonHumansIntermediate Filament ProteinsIntermediate FilamentsLaminsModels, MolecularMutationConceptsIntermediate filamentsAssembly mechanismVariable N-terminalMultiple cellular processesCentral rod domainIntermediate filament structureCoil 1BCellular processesStudy of keratinsTail domainFilament assemblyRod domainC-terminalN-terminalElectrostatic surfacePathologic mutationsKey playersFilament structureRecent insightsComplex formationProteinHuman tissuesGlial fibrillary acidic proteinAcidic proteinDomainPlasma neurofilament light predicts mortality in patients with stroke
Gendron TF, Badi MK, Heckman MG, Jansen-West KR, Vilanilam GK, Johnson PW, Burch AR, Walton RL, Ross OA, Brott TG, Miller TM, Berry JD, Nicholson KA, Wszolek ZK, Oskarsson BE, Sheth KN, Sansing LH, Falcone GJ, Cucchiara BL, Meschia JF, Petrucelli L. Plasma neurofilament light predicts mortality in patients with stroke. Science Translational Medicine 2020, 12 PMID: 33177179, PMCID: PMC9534269, DOI: 10.1126/scitranslmed.aay1913.Peer-Reviewed Original ResearchConceptsBlood neurofilament lightNeurofilament lightStroke typeNeuroaxonal injuryHigher NfL concentrationsMini-Mental State ExaminationStroke brain injuryAcute cerebral infarctionModified Rankin ScaleEarly ischemic injuryNontraumatic intracerebral hemorrhageNIH Stroke ScaleBrain tissue damagePlasma neurofilament lightIntracranial hemorrhage volumeClinical trial efficiencyBlood biomarker studiesStroke therapeuticsCause mortalityStroke ScaleCerebral infarctionRankin ScaleFunctional disabilityIntracerebral hemorrhageIschemic injuryStructural properties of target binding by profilaggrin A and B domains and other S100 fused-type calcium-binding proteins
Hinbest AJ, Kim SR, Eldirany SA, Lomakin IB, Watson J, Ho M, Bunick CG. Structural properties of target binding by profilaggrin A and B domains and other S100 fused-type calcium-binding proteins. Journal Of Dermatological Science 2020, 100: 39-49. PMID: 32893105, PMCID: PMC7752840, DOI: 10.1016/j.jdermsci.2020.08.009.Peer-Reviewed Original ResearchAmino Acid SequenceAnnexin A2Binding SitesCrystallography, X-RayFilaggrin ProteinsHumansHydrophobic and Hydrophilic InteractionsIntermediate Filament ProteinsIntermediate FilamentsKeratinocytesKeratinsMolecular Docking SimulationMutationProtein BindingProtein Conformation, alpha-HelicalProtein DomainsProtein PrecursorsRecombinant ProteinsS100 ProteinsCrystal Structure of Keratin 1/10(C401A) 2B Heterodimer Demonstrates a Proclivity for the C-Terminus of Helix 2B to Form Higher Order Molecular Contacts.
Lomakin IB, Hinbest AJ, Ho M, Eldirany SA, Bunick CG. Crystal Structure of Keratin 1/10(C401A) 2B Heterodimer Demonstrates a Proclivity for the C-Terminus of Helix 2B to Form Higher Order Molecular Contacts. The Yale Journal Of Biology And Medicine 2020, 93: 3-17. PMID: 32226330, PMCID: PMC7087056.Peer-Reviewed Original ResearchThe Interface between Keratin Structurotype and Human Disease
Eldirany SA, Ho M, Bunick CG. The Interface between Keratin Structurotype and Human Disease. Structure 2020, 28: 271-273. PMID: 32130887, PMCID: PMC7252399, DOI: 10.1016/j.str.2020.02.002.Peer-Reviewed Original Research
2019
Rudhira/BCAS3 couples microtubules and intermediate filaments to promote cell migration for angiogenic remodeling
Joshi D, Inamdar M. Rudhira/BCAS3 couples microtubules and intermediate filaments to promote cell migration for angiogenic remodeling. Molecular Biology Of The Cell 2019, 30: 1437-1450. PMID: 30995157, PMCID: PMC6724693, DOI: 10.1091/mbc.e18-08-0484.Peer-Reviewed Original ResearchConceptsCell migrationCytoskeleton organizationMT stabilityIntermediate filamentsEC migrationCytoskeletal protein essentialMT-IFVimentin intermediate filamentsFocal adhesionsEndothelial cell migrationMouse developmentProtein essentialRudhiraAssign functionCytoskeleton modulationDynamic remodelingBlood vessel formationAngiogenic sproutingSprouting angiogenesisAngiogenic remodelingMicrotubulesEssential roleVessel formationSequence 3Migration
2015
Epigenetic silencing of neurofilament genes promotes an aggressive phenotype in breast cancer
Calmon MF, Jeschke J, Zhang W, Dhir M, Siebenkäs C, Herrera A, Tsai HC, O'Hagan HM, Pappou EP, Hooker CM, Fu T, Schuebel KE, Gabrielson E, Rahal P, Herman JG, Baylin SB, Ahuja N. Epigenetic silencing of neurofilament genes promotes an aggressive phenotype in breast cancer. Epigenetics 2015, 10: 622-632. PMID: 25985363, PMCID: PMC4622480, DOI: 10.1080/15592294.2015.1050173.Peer-Reviewed Original ResearchConceptsNeurofilament medium polypeptideNeurofilament heavy polypeptideDNA methylation-associated silencingDNA methylation-mediated silencingNeurofilament genesMethylation-mediated silencingMethylation-associated silencingMethylation-mediated inactivationGo/G1 phaseEpigenetic silencingMedium polypeptideEpigenetic inactivationCell cycleMajor subunitBreast cancer cellsCell typesGenesSilencingHeavy polypeptideG1 phaseFunctional significanceCandidate DNAMature neuronsCancer cellsPolypeptideFrequent somatic reversion of KRT1 mutations in ichthyosis with confetti
Choate KA, Lu Y, Zhou J, Elias PM, Zaidi S, Paller AS, Farhi A, Nelson-Williams C, Crumrine D, Milstone LM, Lifton RP. Frequent somatic reversion of KRT1 mutations in ichthyosis with confetti. Journal Of Clinical Investigation 2015, 125: 1703-1707. PMID: 25774499, PMCID: PMC4396494, DOI: 10.1172/jci64415.Peer-Reviewed Original ResearchAdultAge of OnsetAmino Acid SequenceCell Line, TumorCell NucleusChildChild, PreschoolChromosomes, Human, Pair 12CytoskeletonFrameshift MutationHumansIchthyosisIntermediate FilamentsKeratin-1KeratinocytesLoss of HeterozygosityMaleMolecular Sequence DataMosaicismPhenotypePolymorphism, Single NucleotideProtein TransportTransfection
2013
Somatic HRAS p.G12S Mutation Causes Woolly Hair and Epidermal Nevi
Levinsohn JL, Teng J, Craiglow BG, Loring EC, Burrow TA, Mane SS, Overton JD, Lifton RP, McNiff JM, Lucky AW, Choate KA. Somatic HRAS p.G12S Mutation Causes Woolly Hair and Epidermal Nevi. Journal Of Investigative Dermatology 2013, 134: 1149-1152. PMID: 24129065, PMCID: PMC3961553, DOI: 10.1038/jid.2013.430.Peer-Reviewed Original Research
2010
Mitotic Recombination in Patients with Ichthyosis Causes Reversion of Dominant Mutations in KRT10
Choate KA, Lu Y, Zhou J, Choi M, Elias PM, Farhi A, Nelson-Williams C, Crumrine D, Williams ML, Nopper AJ, Bree A, Milstone LM, Lifton RP. Mitotic Recombination in Patients with Ichthyosis Causes Reversion of Dominant Mutations in KRT10. Science 2010, 330: 94-97. PMID: 20798280, PMCID: PMC3085938, DOI: 10.1126/science.1192280.Peer-Reviewed Original ResearchAmino Acid SequenceCell NucleolusChromosome MappingChromosomes, Human, Pair 17FemaleFrameshift MutationHumansIchthyosiform Erythroderma, CongenitalIntermediate FilamentsKeratin-10KeratinsLoss of HeterozygosityMaleMitosisMolecular Sequence DataMosaicismMutant ProteinsRecombination, GeneticSelection, GeneticSkin
2004
Whorls of Intermediate Filaments With Entrapped Neurosecretory Granules Correspond to the “Rhabdoid” Inclusions Seen in Pancreatic Endocrine Neoplasms
Shia J, Erlandson R, Klimstra D. Whorls of Intermediate Filaments With Entrapped Neurosecretory Granules Correspond to the “Rhabdoid” Inclusions Seen in Pancreatic Endocrine Neoplasms. The American Journal Of Surgical Pathology 2004, 28: 271-273. PMID: 15043320, DOI: 10.1097/00000478-200402000-00018.Peer-Reviewed Original Research
2003
PECAM-1: old friend, new partners
Ilan N, Madri JA. PECAM-1: old friend, new partners. Current Opinion In Cell Biology 2003, 15: 515-524. PMID: 14519385, DOI: 10.1016/s0955-0674(03)00100-5.Peer-Reviewed Original ResearchMeSH KeywordsAdherens Junctionsalpha CateninAnimalsApoptosisbeta CateninCapillary PermeabilityCell Adhesion MoleculesCytoskeletal ProteinsDNA-Binding ProteinsHumansIntermediate FilamentsMilk ProteinsModels, MolecularPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Protein Structure, TertiarySignal TransductionSTAT5 Transcription FactorTrans-ActivatorsVascular Endothelial Growth Factor A
2002
Intermediate filament–membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength
Huen A, Park J, Godsel L, Chen X, Bannon L, Amargo E, Hudson T, Mongiu A, Leigh I, Kelsell D, Gumbiner B, Green K. Intermediate filament–membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength. Journal Of Cell Biology 2002, 159: 1005-1017. PMID: 12499357, PMCID: PMC2173978, DOI: 10.1083/jcb.200206098.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsBiotinylationCadherinsCell AdhesionCell LineCell MembraneCytoskeletal ProteinsCytoskeletonDesmogleinsDesmoplakinsDesmosomesDetergentsDNA, ComplementaryGreen Fluorescent ProteinsHumansIntermediate FilamentsKeratinocytesKeratoderma, PalmoplantarL-Lactate DehydrogenaseLuminescent ProteinsMicroscopy, FluorescenceProtein BindingProtein Structure, TertiaryTime FactorsTransfectionTumor Cells, CulturedConceptsMembrane attachmentAdherens junctionsIntermediate filamentsIntercellular adhesive strengthCell surface distributionDetergent-insoluble poolIF cytoskeletonPlasma membraneIntercellular adhesionLatrunculin AActin filamentsJunctional plaquesA431 cellsTerminal truncationDesmosomal proteinsCell aggregatesTissue integrityWeakly adherentLactate dehydrogenase releaseReduced adhesionFacilitate formationCell sheetsNormal keratinocytesSpecific decreaseDehydrogenase release
2001
Anomalous apical plasma membrane phenotype in CK8-deficient mice indicates a novel role for intermediate filaments in the polarization of simple epithelia
Ameen N, Figueroa Y, Salas P. Anomalous apical plasma membrane phenotype in CK8-deficient mice indicates a novel role for intermediate filaments in the polarization of simple epithelia. Journal Of Cell Science 2001, 114: 563-575. PMID: 11171325, DOI: 10.1242/jcs.114.3.563.Peer-Reviewed Original ResearchConceptsIntermediate filamentsPolarized epithelial cellsApical membrane proteinsEpithelial cellsSyntaxin 3Apical domainFemale sterilityMembrane proteinsApical markerNovel functionGamma-tubulinNovel roleApical poleSimple epitheliaCell typesColorectal hyperplasiaCK intermediate filamentsNull micePhenotypeBasolateral levelsNecrotic cellsMembrane phenotypeCellsFilamentsCytoplasm of enterocytes
2000
Plasticin, a Type III Neuronal Intermediate Filament Protein, Assembles as an Obligate Heteropolymer
Asch WS, Schechter N. Plasticin, a Type III Neuronal Intermediate Filament Protein, Assembles as an Obligate Heteropolymer. Journal Of Neurochemistry 2000, 75: 1475-1486. PMID: 10987827, DOI: 10.1046/j.1471-4159.2000.0751475.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAnimalsAxonsBiopolymersCatalysisCell LineEpidermolysis Bullosa SimplexEye ProteinsHemagglutininsHumansImmunohistochemistryIntermediate Filament ProteinsIntermediate FilamentsMutagenesis, Site-DirectedNerve Tissue ProteinsNeuronsPhenotypeRecombinant Fusion ProteinsTemperatureTransfectionVimentinZebrafishConceptsIntermediate filament proteinsEpidermolysis bullosa simplexPoint mutationsMammalian intermediate filament proteinsFilament proteinsCytoplasmic filament networkNeuronal intermediate filament proteinWeight NF proteinsTermination sequenceSW13 cellsObligate heteropolymersStructural differencesFilament networkCoil regionFilament aggregationKeratin mutationsMutationsImportant structural differencesAssembly propertiesProteinNF proteinsFirst heptadNumber of aggregatesPlasticinGrowth characteristics
1993
Protection of the axonal cytoskeleton in anoxic optic nerve by decreased extracellular calcium
Waxman S, Black J, Ransom B, Stys P. Protection of the axonal cytoskeleton in anoxic optic nerve by decreased extracellular calcium. Brain Research 1993, 614: 137-145. PMID: 8348309, DOI: 10.1016/0006-8993(93)91027-p.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsCalciumCytoskeletonHypoxia, BrainIn Vitro TechniquesIntermediate FilamentsMicrotubulesNerve Fibers, MyelinatedOptic NervePerfusionRanvier's NodesRatsConceptsArtificial cerebrospinal fluidMin of anoxiaOptic nerveZero-Ca2White matterAnoxic injuryCNS white matter tractAxonal cytoskeletonOptic nerve axonsCNS white matterRat optic nerveInflux of Ca2White matter tractsLoss of cristaeDisorganization of cristaeMembranous profilesUltrastructure of axonsAbnormal influxCerebrospinal fluidExtracellular calciumNerveMyelinated axonsNerve axonsNormal Ca2Axons
1986
Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum.
Schwartz M, Luna E. Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum. Journal Of Cell Biology 1986, 102: 2067-2075. PMID: 2423531, PMCID: PMC2114255, DOI: 10.1083/jcb.102.6.2067.Peer-Reviewed Original Research
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