2025
Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM
Renganathan B, Moore A, Yeo W, Petruncio A, Ackerman D, Weigel A, Team T, Pasolli H, Xu C, Shtengel G, Hess H, Serpinskaya A, Zhang H, Lippincott-Schwartz J, Gelfand V. Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM. Journal Of Cell Biology 2025, 224: e202406054. PMID: 40062969, PMCID: PMC11893169, DOI: 10.1083/jcb.202406054.Peer-Reviewed Original Research
2022
Extracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells
Amraei R, Xia C, Olejnik J, White M, Napoleon M, Lotfollahzadeh S, Hauser B, Schmidt A, Chitalia V, Mühlberger E, Costello C, Rahimi N. Extracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2113874119. PMID: 35078919, PMCID: PMC8833221, DOI: 10.1073/pnas.2113874119.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionHuman endothelial cellsSARS-CoV-2 entrySARS-CoV-2S-protein interactionEndothelial cellsIdentification of vimentinShRNA-mediated knockdownIntermediate filament proteinsBinding of vimentinHEK-293 cellsAttachment factorsViral entrySARS-CoV-2 S proteinDevelopment of therapeuticsExtracellular vimentinS protein receptorInfectious SARS-CoV-2Host cellsCellular componentsCoexpression of vimentinFilament proteinsPrimary entry receptorSARS-CoV-2 spike proteinS protein
2020
Anti-modified citrullinated vimentin antibody: a novel biomarker associated with cardiac systolic dysfunction in patients with rheumatoid arthritis
Norouzi S, Javinani A, Aminorroaya A, Masoumi M. Anti-modified citrullinated vimentin antibody: a novel biomarker associated with cardiac systolic dysfunction in patients with rheumatoid arthritis. BMC Cardiovascular Disorders 2020, 20: 390. PMID: 32847506, PMCID: PMC7448352, DOI: 10.1186/s12872-020-01676-x.Peer-Reviewed Original ResearchConceptsLeft ventricular ejection fractionCardiac systolic dysfunctionCardiovascular diseaseSystolic dysfunctionRheumatoid arthritisReduced left ventricular ejection fractionPredictors of left ventricular ejection fractionAnti-modified citrullinated vimentinEchocardiographic parameters of systolicDisease Activity Score-28Anti-MCV levelsParameters of systolicAssociation of autoantibodiesVentricular ejection fractionConcomitant cardiovascular diseaseExclusion of patientsSusceptibility to cardiovascular diseaseReceiver operating characteristic curveDuration of diseaseCardiovascular disease risk factorsAnti-citrullinated proteinFramingham risk scoreCross-sectional studyTransthoracic echocardiographyDiastolic dysfunction
2019
Protective effects of GPR120 agonist-programmed macrophages on renal interstitial fibrosis in unilateral ureteral obstruction (UUO) rats
Wang L, Ren X, Tian XF, Cheng XL, Zhao YY, Li QY, Duan ZY, Tian LF, Chen Z, Lu JM, Liang XY, Zhao YF, Fu RG. Protective effects of GPR120 agonist-programmed macrophages on renal interstitial fibrosis in unilateral ureteral obstruction (UUO) rats. Biomedicine & Pharmacotherapy 2019, 117: 109172. PMID: 31261028, DOI: 10.1016/j.biopha.2019.109172.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsbeta CateninBiphenyl CompoundsCytokinesFibrosisGene Expression RegulationKidney DiseasesMacrophages, PeritonealMaleModels, BiologicalPhenotypePhenylpropionatesProtective AgentsRats, Sprague-DawleyReceptors, G-Protein-CoupledSignal TransductionTransforming Growth Factor beta1Ureteral ObstructionVimentinConceptsRenal interstitial fibrosisUnilateral ureteral obstructionInterstitial fibrosisUreteral obstructionProtective effectFree fatty acid receptor GPR120Fatty acid receptor GPR120Unilateral ureteral obstruction operationPeritoneal macrophagesAbnormal expressionExpression of CD206M2 phenotype macrophagesTumor necrosis factorEpithelial-mesenchymal transitionAutologous administrationReceptor GPR120UUO ratsInterleukin-6Intrarenal injectionArginase-1Necrosis factorGPR120 agonistM2 phenotypeΑ-SMATGF-β1Rudhira/BCAS3 couples microtubules and intermediate filaments to promote cell migration for angiogenic remodeling
Joshi D, Inamdar M. Rudhira/BCAS3 couples microtubules and intermediate filaments to promote cell migration for angiogenic remodeling. Molecular Biology Of The Cell 2019, 30: 1437-1450. PMID: 30995157, PMCID: PMC6724693, DOI: 10.1091/mbc.e18-08-0484.Peer-Reviewed Original ResearchConceptsCell migrationCytoskeleton organizationMT stabilityIntermediate filamentsEC migrationCytoskeletal protein essentialMT-IFVimentin intermediate filamentsFocal adhesionsEndothelial cell migrationMouse developmentProtein essentialRudhiraAssign functionCytoskeleton modulationDynamic remodelingBlood vessel formationAngiogenic sproutingSprouting angiogenesisAngiogenic remodelingMicrotubulesEssential roleVessel formationSequence 3Migration
2016
Circulating Tumor Cell Phenotype Predicts Recurrence and Survival in Pancreatic Adenocarcinoma
Poruk KE, Valero V, Saunders T, Blackford AL, Griffin JF, Poling J, Hruban RH, Anders RA, Herman J, Zheng L, Rasheed ZA, Laheru DA, Ahuja N, Weiss MJ, Cameron JL, Goggins M, Iacobuzio-Donahue CA, Wood LD, Wolfgang CL. Circulating Tumor Cell Phenotype Predicts Recurrence and Survival in Pancreatic Adenocarcinoma. Annals Of Surgery 2016, 264: 1073-1081. PMID: 26756760, PMCID: PMC4936958, DOI: 10.1097/sla.0000000000001600.Peer-Reviewed Original ResearchConceptsCytokeratin-positive CTCsPancreatic adenocarcinomaMesenchymal markersVimentin-positive CTCsEpithelial tumor cells (ISET) methodFourth leading causeBetter treatment stratificationPortal blood samplesSignificant independent predictorsPotential prognostic biomarkerBiology of metastasisDetection of CTCsSurgical resectionIndependent predictorsPrognostic factorsMedian timeMultivariable analysisPDAC patientsPrognostic utilityCancer deathPatient prognosisLeading causeTreatment stratificationCancer recurrencePoor survival
2015
Nonalcoholic fatty liver disease induced by noncanonical Wnt and its rescue by Wnt3a
Wang S, Song K, Srivastava R, Dong C, Go G, Li N, Iwakiri Y, Mani A. Nonalcoholic fatty liver disease induced by noncanonical Wnt and its rescue by Wnt3a. The FASEB Journal 2015, 29: 3436-3445. PMID: 25917329, PMCID: PMC4511193, DOI: 10.1096/fj.15-271171.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell Line, TumorCell TransdifferentiationFatty LiverHep G2 CellsHepatocytesHumansLiverLow Density Lipoprotein Receptor-Related Protein-6MiceMice, Inbred C57BLNon-alcoholic Fatty Liver DiseaseProtein BindingProtein Kinase CProtein Kinase C-alpharho-Associated KinasesSignal TransductionTransforming Growth Factor beta1VimentinWnt Signaling PathwayWnt3A ProteinConceptsNonalcoholic fatty liver diseaseFatty liver diseaseNonalcoholic steatohepatitisLiver diseaseLDL receptor-related protein 6NASH-related liver diseaseMetabolic risk factorsChronic liver diseaseEarly-onset atherosclerosisImportant potential therapeutic targetTGF-β1 activityPotential therapeutic targetDisease pathwaysRas homolog family member ASmooth muscle αFamily member ARisk factorsDisease progressionCommon causeLRP6 knockdownTherapeutic targetWnt3a administrationHepatocyte transdifferentiationDiseaseMuscle α
2014
Casein kinase 2 prevents mesenchymal transformation by maintaining Foxc2 in the cytoplasm
Golden D, Cantley LG. Casein kinase 2 prevents mesenchymal transformation by maintaining Foxc2 in the cytoplasm. Oncogene 2014, 34: 4702-4712. PMID: 25486430, PMCID: PMC4459945, DOI: 10.1038/onc.2014.395.Peer-Reviewed Original ResearchConceptsCK2β regulatory subunitSerine 124Epithelial-mesenchymal transitionRegulatory subunitNuclear localizationCytoplasmic localizationCasein kinase 2 phosphorylation sitesDevelopmental epithelial-mesenchymal transitionMesenchymal transformationCell migrationEpithelial cellsDe novo accumulationCK2 associatesTranscriptional regulatorsMalignant breast cancer cellsPhosphorylation sitesCK2β expressionKinase subunitMesenchymal genesBreast epithelial cellsProper targetingAmino terminusNormal breast epithelial cellsNormal epithelial cellsLeucine promotesReversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model
Kunjithapatham R, Karthikeyan S, Geschwind JF, Kieserman E, Lin M, Fu DX, Ganapathy-Kanniappan S. Reversal of Anchorage-Independent Multicellular Spheroid into a Monolayer Mimics a Metastatic Model. Scientific Reports 2014, 4: 6816. PMID: 25351825, PMCID: PMC4212233, DOI: 10.1038/srep06816.Peer-Reviewed Original ResearchConceptsMetastatic phenotypeAnchorage-independent growthMolecular regulationMulticellular spheroidsStem cell markersMolecular eventsCancer stem cell markersVitro modelModel of metastasisMetastatic processSpecific therapeutic targetsTherapeutic targetAntimetastatic agentPhenotypeMetastatic modelRegulationVivo dataExpressionChemoresistanceInvasivenessSpheroidsMajor impedimentInductionMimicsGrowthMechanotransduction of shear stress occurs through changes in VE-cadherin and PECAM-1 tension: Implications for cell migration
Conway DE, Schwartz MA. Mechanotransduction of shear stress occurs through changes in VE-cadherin and PECAM-1 tension: Implications for cell migration. Cell Adhesion & Migration 2014, 9: 335-339. PMID: 25482618, PMCID: PMC4955370, DOI: 10.4161/19336918.2014.968498.Peer-Reviewed Original ResearchCharacterization and development of photoactivatable fluorescent proteins for single-molecule–based superresolution imaging
Wang S, Moffitt JR, Dempsey GT, Xie XS, Zhuang X. Characterization and development of photoactivatable fluorescent proteins for single-molecule–based superresolution imaging. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 8452-8457. PMID: 24912163, PMCID: PMC4060684, DOI: 10.1073/pnas.1406593111.Peer-Reviewed Original ResearchAnimalsBacterial ProteinsBlotting, WesternCell Line, TumorChlorocebus aethiopsCOS CellsDNA-Binding ProteinsEscherichia coli ProteinsHumansLuminescent ProteinsMicroscopy, FluorescencePhotonsProtein MultimerizationReceptors, Cell SurfaceRecombinant Fusion ProteinsSpectrometry, FluorescenceVimentinZyxinHeterogeneous atypical cell populations are present in blood of metastatic breast cancer patients
Lustberg M, Balasubramanian P, Miller B, Garcia-Villa A, Deighan C, Wu Y, Carothers S, Berger M, Ramaswamy B, Macrae E, Wesolowski R, Layman R, Mrozek E, Pan X, Summers T, Shapiro C, Chalmers J. Heterogeneous atypical cell populations are present in blood of metastatic breast cancer patients. Breast Cancer Research 2014, 16: r23. PMID: 24602188, PMCID: PMC4053256, DOI: 10.1186/bcr3622.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAntigens, CDAntigens, Differentiation, MyelomonocyticAntigens, NeoplasmBiomarkers, TumorBreast NeoplasmsCell Adhesion MoleculesCell Line, TumorEpithelial Cell Adhesion MoleculeErbB ReceptorsFemaleFlow CytometryHumansImmunohistochemistryKeratin-18Keratin-19Keratin-8Leukocyte Common AntigensMCF-7 CellsMicroscopy, ConfocalMiddle AgedNeoplasm MetastasisNeoplastic Cells, CirculatingPrognosisProspective StudiesVimentinConceptsMetastatic breast cancerBreast cancer patientsBlood samplesCancer patientsBreast cancerMetastatic breast cancer patientsPatient samplesMultiparametric flow cytometry analysisAtypical cell populationNumber of CKPresent prospective trialWorse overall survivalTumor-associated macrophagesCell populationsPan-hematopoietic marker CD45Confocal microscopyEpidermal growth factor receptorEpithelial cell adhesion moleculeNormal control samplesCell surface markersRole of EpCAMFlow cytometry analysisGrowth factor receptorOverall survivalProspective trial
2013
Cartilage Oligomeric Matrix Protein in Idiopathic Pulmonary Fibrosis
Vuga LJ, Milosevic J, Pandit K, Ben-Yehudah A, Chu Y, Richards T, Sciurba J, Myerburg M, Zhang Y, Parwani AV, Gibson KF, Kaminski N. Cartilage Oligomeric Matrix Protein in Idiopathic Pulmonary Fibrosis. PLOS ONE 2013, 8: e83120. PMID: 24376648, PMCID: PMC3869779, DOI: 10.1371/journal.pone.0083120.Peer-Reviewed Original ResearchMeSH KeywordsAgedCartilage Oligomeric Matrix ProteinCells, CulturedCollagen Type ICollagen Type I, alpha 1 ChainExtracellular MatrixFemaleFibroblastsGene Expression RegulationHumansIdiopathic Pulmonary FibrosisLungMaleMiddle AgedPlasminogen Activator Inhibitor 1RNA, Small InterferingSignal TransductionSmad3 ProteinTransforming Growth Factor beta1VimentinConceptsIdiopathic pulmonary fibrosisCartilage oligomeric matrix proteinIPF lungsNormal human lung fibroblastsForce vital capacityHuman lung fibroblastsTGF-β1Oligomeric matrix proteinPulmonary fibrosisLung fibroblastsSerum COMP concentrationTGF-β1 activityEpithelial cell hyperplasiaMatrix proteinsLung restrictionWestern blot analysisExtracellular matrix depositionIPF patientsTime-dependent fashionDisease activityMedian survivalVital capacityCell hyperplasiaControl lungsBlood drawAntibodies to citrullinated peptides in tuberculosis
Lima I, Oliveira R, Atta A, Marchi S, Barbosa L, Reis E, Reis M, Santiago M. Antibodies to citrullinated peptides in tuberculosis. Clinical Rheumatology 2013, 32: 685-687. PMID: 23344687, DOI: 10.1007/s10067-013-2173-y.Peer-Reviewed Original ResearchConceptsAnti-citrullinated peptide antibodiesAnti-MCV antibodiesRheumatoid arthritisHealthy controlsRheumatoid factor positivityRA patientsSymmetric polyarthritisTB patientsPulmonary tuberculosisBone erosionCCP antibodyAutoimmune diseasesHigh incidencePeptide antibodiesPatientsControl groupHigh specificityTuberculosisInfectious diseasesAntibodiesPositivityDiseaseSpecificityMethodological factorsPolyarthritis
2012
Toll‐Like Receptor 4 Expression in Decidual Cells and Interstitial Trophoblasts Across Human Pregnancy
Schatz F, Kayisli UA, Vatandaslar E, Ocak N, Guller S, Abrahams VM, Krikun G, Lockwood CJ. Toll‐Like Receptor 4 Expression in Decidual Cells and Interstitial Trophoblasts Across Human Pregnancy. American Journal Of Reproductive Immunology 2012, 68: 146-153. PMID: 22564191, PMCID: PMC3395732, DOI: 10.1111/j.1600-0897.2012.01148.x.Peer-Reviewed Original ResearchConceptsToll-like receptor 4Interstitial trophoblastDecidual cellsDanger signalsToll-like receptor 4 expressionFirst trimester decidual cellsReceptor 4 expressionHuman pregnancyElective terminationNormal deliveryDecidua parietalisReceptor 4Luteal phaseStromal cellsGram-negative bacteriaSerial sectionsTrophoblastPrimary targetCellsClose contactEndometriumPregnancyHScoreGestationCytokeratinCyclooxygenase‐2 and Akt mediate multiple growth‐factor‐induced epithelial‐mesenchymal transition in human hepatocellular carcinoma
Ogunwobi O, Wang T, Zhang L, Liu C. Cyclooxygenase‐2 and Akt mediate multiple growth‐factor‐induced epithelial‐mesenchymal transition in human hepatocellular carcinoma. Journal Of Gastroenterology And Hepatology 2012, 27: 566-578. PMID: 22097969, PMCID: PMC3288221, DOI: 10.1111/j.1440-1746.2011.06980.x.Peer-Reviewed Original ResearchMeSH KeywordsAlbuminsalpha 1-AntitrypsinAnimalsCadherinsCarcinoma, HepatocellularCell MovementCell TransplantationCollagen Type ICyclooxygenase 2DinoprostoneEpidermal Growth FactorEpithelial-Mesenchymal TransitionFibroblast Growth Factor 2FibronectinsGene ExpressionHepatocyte Growth FactorHumansMiceOncogene Protein v-aktRNA, Small InterferingSignal TransductionTransforming Growth Factor beta1Tumor Cells, CulturedVimentinConceptsEpithelial-mesenchymal transitionCyclooxygenase-2Hepatocellular carcinomaBasic fibroblast growth factorGrowth factorProstaglandin E2Metastatic hepatocellular carcinomaProgression of HCCEffective therapeutic strategyExpression of vimentinHepatocyte growth factorGrowth factor βHuman hepatocellular carcinomaFibroblast growth factorAssociated hepatitisChemopreventive strategiesEpidermal growth factorMultiple growth factorsTherapeutic strategiesMesenchymal changesSignificant mortalityAkt pathwayMolecular targetingCancer invasionAkt
2011
Low doses ionizing radiation enhances the invasiveness of breast cancer cells by inducing epithelial–mesenchymal transition
Zhang X, Li X, Zhang N, Yang Q, Moran MS. Low doses ionizing radiation enhances the invasiveness of breast cancer cells by inducing epithelial–mesenchymal transition. Biochemical And Biophysical Research Communications 2011, 412: 188-192. PMID: 21810413, DOI: 10.1016/j.bbrc.2011.07.074.Peer-Reviewed Original ResearchConceptsEpithelial-mesenchymal transitionBreast cancer cellsMCF-7 cellsCancer cellsMCF-7 breast cancer cellsEpithelial differentiation markersBreast cancerEMT markersLow doseMesenchymal markersTherapeutic effectivenessTranswell assaysMolecular alterationsLow dosesMesenchymal transitionRT-PCRCell invasionEnhanced migrationDifferentiation markersLDIRInvasivenessMarkersCellsPhenotypic changesInvasionHepatocyte growth factor upregulation promotes carcinogenesis and epithelial-mesenchymal transition in hepatocellular carcinoma via Akt and COX-2 pathways
Ogunwobi O, Liu C. Hepatocyte growth factor upregulation promotes carcinogenesis and epithelial-mesenchymal transition in hepatocellular carcinoma via Akt and COX-2 pathways. Clinical & Experimental Metastasis 2011, 28: 721-731. PMID: 21744257, PMCID: PMC3732749, DOI: 10.1007/s10585-011-9404-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCadherinsCarcinoma, HepatocellularCell AdhesionCell DifferentiationCell Line, TumorCell MovementCell ProliferationCyclooxygenase 2Enzyme-Linked Immunosorbent AssayEpithelial-Mesenchymal TransitionExtracellular Signal-Regulated MAP KinasesHepatocyte Growth FactorLiver Neoplasms, ExperimentalMiceMice, Inbred BALB CNeoplasm InvasivenessPhosphorylationProto-Oncogene Proteins c-aktReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSignal TransductionUp-RegulationVimentinConceptsEpithelial-mesenchymal transitionHepatocyte growth factorCyclooxygenase-2Hepatocellular carcinomaBNL cellsMarkers of EMTDevelopment of HCCAdvanced hepatocellular carcinomaCOX-2 pathwayMetastatic hepatocellular carcinomaUpregulation of HGFMesenchymal characteristicsGrowth factor upregulationE-cadherinCharacteristic epithelial morphologyCancer mortalitySubsequent metastasisEMT markersImportant causeMigratory capacityHCC cellsBNL CLCancer progressionCollagen 1Growth factorDifferential Acute and Chronic Effects of Leptin on Hypothalamic Astrocyte Morphology and Synaptic Protein Levels
García-Cáceres C, Fuente-Martín E, Burgos-Ramos E, Granado M, Frago LM, Barrios V, Horvath T, Argente J, Chowen JA. Differential Acute and Chronic Effects of Leptin on Hypothalamic Astrocyte Morphology and Synaptic Protein Levels. Endocrinology 2011, 152: 1809-1818. PMID: 21343257, PMCID: PMC3860256, DOI: 10.1210/en.2010-1252.Peer-Reviewed Original ResearchConceptsGlial fibrillary acidic proteinChronic leptin exposureSynaptic inputsAstrocyte morphologyLeptin exposureGFAP levelsGlial structural proteinsSynaptic protein densityChronic leptin administrationAcute leptin treatmentSynaptic protein levelsAdult male ratsCentral leptin resistanceFibrillary acidic proteinLevels 1 hPossible direct effectGlial ensheathingNeonatal overnutritionGlial activationLeptin levelsLeptin administrationHypothalamic neuronsLeptin resistanceLeptin treatmentMale ratsAccreta complicating complete placenta previa is characterized by reduced systemic levels of vascular endothelial growth factor and by epithelial-to-mesenchymal transition of the invasive trophoblast
Wehrum MJ, Buhimschi IA, Salafia C, Thung S, Bahtiyar MO, Werner EF, Campbell KH, Laky C, Sfakianaki AK, Zhao G, Funai EF, Buhimschi CS. Accreta complicating complete placenta previa is characterized by reduced systemic levels of vascular endothelial growth factor and by epithelial-to-mesenchymal transition of the invasive trophoblast. American Journal Of Obstetrics And Gynecology 2011, 204: 411.e1-411.e11. PMID: 21316642, PMCID: PMC3136625, DOI: 10.1016/j.ajog.2010.12.027.Peer-Reviewed Original ResearchConceptsVascular endothelial growth factorComplete placenta previaPlacenta previaEndothelial growth factorCytokeratin 7Growth factorExtravillous trophoblastsSystemic vascular endothelial growth factorMesenchymal transitionFree vascular endothelial growth factorInterstitial extravillous trophoblastsMesenchymal transition featuresPlacental growth factorAngiogenic factor profilesExpression of vimentinInvasive extravillous trophoblastsUncomplicated pregnanciesMyometrial invasionPregnant womenPreviaInvasive trophoblastFactor profileHistological specimensInvasive placentationSerum samples
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