2025
Molecular Components of Vesicle Cycling at the Rod Photoreceptor Ribbon Synapse
Hanke-Gogokhia C, Zapadka T, Finkelstein S, Arshavsky V, Demb J. Molecular Components of Vesicle Cycling at the Rod Photoreceptor Ribbon Synapse. Advances In Experimental Medicine And Biology 2025, 1468: 325-330. PMID: 39930217, DOI: 10.1007/978-3-031-76550-6_54.Peer-Reviewed Original ResearchConceptsSynaptic vesicle exocytosisSynaptic vesicle recyclingPhotoreceptor ribbon synapseVesicle exocytosisVesicle recyclingVesicle cycleVesicle releaseRibbon synapseProtein synthesisProperties of synaptic transmissionMolecular componentsMouse rodsSynaptic terminalsRod cellsProteinVesiclesRod photoreceptorsDim lightSynaptic transmissionInner segmentsCellsExocytosisEndocytosisOuter segmentsEnergy production
2024
Glycolysis in hepatic stellate cells coordinates fibrogenic extracellular vesicle release spatially to amplify liver fibrosis
Khanal S, Liu Y, Bamidele A, Wixom A, Washington A, Jalan-Sakrikar N, Cooper S, Vuckovic I, Zhang S, Zhong J, Johnson K, Charlesworth M, Kim I, Yeon Y, Yoon S, Noh Y, Meroueh C, Timbilla A, Yaqoob U, Gao J, Kim Y, Lucien F, Huebert R, Hay N, Simons M, Shah V, Kostallari E. Glycolysis in hepatic stellate cells coordinates fibrogenic extracellular vesicle release spatially to amplify liver fibrosis. Science Advances 2024, 10: eadn5228. PMID: 38941469, PMCID: PMC11212729, DOI: 10.1126/sciadv.adn5228.Peer-Reviewed Original ResearchConceptsHepatic stellate cellsLiver fibrosisExtracellular vesiclesEV releaseHistone 3 lysine 9 acetylationExtracellular vesicle releaseIncreased EV releaseFibrotic gene expressionNanosized extracellular vesiclesPromoter regionVesicle releasePotential therapeutic targetGene expressionGenetic inhibitionSpatial transcriptomicsStellate cellsGlycolysisUp-regulatedFibrosisTherapeutic targetPericentral zoneLiverPathwayAmplificationExpression
2022
Molecular determinants of complexin clamping and activation function
Bera M, Ramakrishnan S, Coleman J, Krishnakumar SS, Rothman JE. Molecular determinants of complexin clamping and activation function. ELife 2022, 11: e71938. PMID: 35442188, PMCID: PMC9020821, DOI: 10.7554/elife.71938.Peer-Reviewed Original ResearchConceptsSynaptotagmin-1Single-vesicle fusionAccessory helixFusion clampHelical domainMolecular detailsComplexinMutational analysisVesicle releaseFusion kineticsMolecular determinantsSpecific interactionsInhibitory functionProbability of fusionRapid CaSNAREpinsAssembly processFusionClamping functionDomainHelixVesiclesFunctionMembraneInteraction
2014
Genetic analysis of the Complexin trans-clamping model for cross-linking SNARE complexes in vivo
Cho RW, Kümmel D, Li F, Baguley SW, Coleman J, Rothman JE, Littleton JT. Genetic analysis of the Complexin trans-clamping model for cross-linking SNARE complexes in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 10317-10322. PMID: 24982161, PMCID: PMC4104896, DOI: 10.1073/pnas.1409311111.Peer-Reviewed Original ResearchConceptsSNARE complexSpontaneous synaptic vesicle fusionSingle SNARE complexSNARE fusion machinerySynaptic vesicle fusionGenetic rescue approachStructure-function studiesDistinct molecular mechanismsVivo genetic manipulationCpx proteinsFusion clampTrans-SNAREFusion machineryTrans interactionsConformational switchGenetic manipulationGenetic analysisVesicle fusionMolecular mechanismsVesicle releaseRescue approachMutantsProteinSnareAdditional mechanismFluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons.
Li H, Park HA, Jonas EA. Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons. Bio-protocol 2014, 4 PMID: 27446978, PMCID: PMC4950945, DOI: 10.21769/bioprotoc.1304.Peer-Reviewed Original Research
2013
Patch-Clamp Recordings from Lateral Line Neuromast Hair Cells of the Living Zebrafish
Ricci AJ, Bai JP, Song L, Lv C, Zenisek D, Santos-Sacchi J. Patch-Clamp Recordings from Lateral Line Neuromast Hair Cells of the Living Zebrafish. Journal Of Neuroscience 2013, 33: 3131-3134. PMID: 23407967, PMCID: PMC3684625, DOI: 10.1523/jneurosci.4265-12.2013.Peer-Reviewed Original ResearchConceptsHair cellsSynaptic vesicle releaseNeuromast hair cellsLiving zebrafishBiological discoveryIonic channel activityZebrafishVesicle releaseChannel activityPhysiological activityLateral lineJunctional couplingCellsDetailed understandingPatch-clamp recordingsVestibular peripheryFishNeuromastsActivityDiscoveryFirst recording
1982
Synaptic vesicle recycling at the neuromuscular junction in the presence of a presynaptic membrane marker
Lentz T, Chester J. Synaptic vesicle recycling at the neuromuscular junction in the presence of a presynaptic membrane marker. Neuroscience 1982, 7: 9-20. PMID: 6176905, DOI: 10.1016/0306-4522(82)90148-8.Peer-Reviewed Original ResearchConceptsSynaptic vesicle recyclingSynaptic vesiclesVesicle recyclingNew synaptic vesiclesAxolemmal infoldingsPresynaptic axonal membraneMechanism of recyclingSynaptic vesicle releaseNeuromuscular junctionBlack widow spider venomMembranous cisternaeVesicle membraneCoated vesiclesVesicle releaseSpider venomAxonal membraneMembrane markersVesiclesMembraneExternal potassium ionsCisternaeInfoldingsRecyclingMarkersBungarotoxin
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