2025
Neuronal potassium channel activity triggers initiation of mRNA translation through binding of translation regulators
Malone T, Wu J, Zhang Y, Licznerski P, Chen R, Nahiyan S, Pedram M, Jonas E, Kaczmarek L. Neuronal potassium channel activity triggers initiation of mRNA translation through binding of translation regulators. Science Advances 2025, 11: eadv3140. PMID: 40435242, PMCID: PMC12118559, DOI: 10.1126/sciadv.adv3140.Peer-Reviewed Original ResearchConceptsMRNA translationTranslational regulationInitiation of mRNA translationInitiation of translationSevere intellectual disabilityRegulation of translationMRNA translation regulationNeurites of cortical neuronsB-actinChannel activityIntellectual disabilityPotassium channel activityNeuronal activityMolecular mechanismsInhibit initiationMutationsCell linesPharmacological stimulationCortical neuronsMRNABindingRegulationTranslationEIF4ECYFIP1Engineering a genomically recoded organism with one stop codon
Grome M, Nguyen M, Moonan D, Mohler K, Gurara K, Wang S, Hemez C, Stenton B, Cao Y, Radford F, Kornaj M, Patel J, Prome M, Rogulina S, Sozanski D, Tordoff J, Rinehart J, Isaacs F. Engineering a genomically recoded organism with one stop codon. Nature 2025, 639: 512-521. PMID: 39910296, PMCID: PMC11903333, DOI: 10.1038/s41586-024-08501-x.Peer-Reviewed Original Research
2022
mRNA 5′ terminal sequences drive 200-fold differences in expression through effects on synthesis, translation and decay
van den Elzen A, Watson M, Thoreen C. mRNA 5′ terminal sequences drive 200-fold differences in expression through effects on synthesis, translation and decay. PLOS Genetics 2022, 18: e1010532. PMID: 36441824, PMCID: PMC9731452, DOI: 10.1371/journal.pgen.1010532.Peer-Reviewed Original ResearchConceptsTerminal sequenceGene expressionKey post-transcriptional regulatorsTerminal oligopyrimidine motifsCore promoter motifsPost-transcriptional regulatorsPromoter motifsMRNA decayTranslation initiationRegulatory sequencesReporter mRNAEfficient transcriptionLibrary sequencesEndogenous mRNARegulatory potentialNative mRNAHuman cellsTranscriptionMRNAHybrid sequencesSequenceExpressionMotifMRNA expressionTranslationAn in Vitro Cytomimetic of In‐Cell RNA Folding
Yoo H, Davis C. An in Vitro Cytomimetic of In‐Cell RNA Folding. ChemBioChem 2022, 23: e202200406. PMID: 35999178, DOI: 10.1002/cbic.202200406.Peer-Reviewed Original ResearchUncovering translation roadblocks during the development of a synthetic tRNA
Prabhakar A, Krahn N, Zhang J, Vargas-Rodriguez O, Krupkin M, Fu Z, Acosta-Reyes FJ, Ge X, Choi J, Crnković A, Ehrenberg M, Puglisi EV, Söll D, Puglisi J. Uncovering translation roadblocks during the development of a synthetic tRNA. Nucleic Acids Research 2022, 50: 10201-10211. PMID: 35882385, PMCID: PMC9561287, DOI: 10.1093/nar/gkac576.Peer-Reviewed Original ResearchConceptsOrthogonal translation systemGenetic code expansionCode expansionTertiary interactionsNon-canonical amino acidsAminoacyl-tRNA substratesDomains of lifeAminoacyl-tRNA synthetaseTranslation systemSingle nucleotide mutationsSingle-molecule fluorescenceDistinct tRNAsNon-canonical structuresSelenocysteine insertionRibosomal translationTRNARibosomesSynthetic tRNANucleotide mutationsAmino acidsSame organismP siteOrganismsTranslocationTranslationMeasuring the tolerance of the genetic code to altered codon size
DeBenedictis EA, Söll D, Esvelt KM. Measuring the tolerance of the genetic code to altered codon size. ELife 2022, 11: e76941. PMID: 35293861, PMCID: PMC9094753, DOI: 10.7554/elife.76941.Peer-Reviewed Original ResearchConceptsFour-base codonsGenetic codeTRNA mutationsAminoacyl-tRNA synthetasesQuadruplet codonsSingle amino acidCodon translationTriplet codonsTRNA synthetasesSynthetic biologistsCodonTRNAAmino acidsChemical alphabetsMutationsMass spectrometrySynthetasesAnticodonToleranceSynthetic systemsBiologistsTranslationEscherichiaNascentDifferential synthesis of novel small protein times Salmonella virulence program
Salvail H, Choi J, Groisman EA. Differential synthesis of novel small protein times Salmonella virulence program. PLOS Genetics 2022, 18: e1010074. PMID: 35245279, PMCID: PMC8896665, DOI: 10.1371/journal.pgen.1010074.Peer-Reviewed Original ResearchConceptsVirulence programPhoP/PhoQCold-blooded animalsSalmonella enterica serovar TyphimuriumAncestral regulatorGene organizationBicistronic operonEnterica serovar TyphimuriumPolycistronic mRNACellular processesGene controlCorresponding proteinMRNA speciesOperonPhoP phosphorylationGenesSerovar TyphimuriumDifferential synthesisPremature activationS. entericaMRNATranslationUgtLPhoQRibosomesThe Process of Translating and Culturally Adapting a Digital Elder Abuse Intervention
Garcia M, Pena-Solorzano D, Edwards C, Abujarad F. The Process of Translating and Culturally Adapting a Digital Elder Abuse Intervention. International Journal Of Translation And Interpretation Studies 2022, 2: 07-26. DOI: 10.32996/ijtis.2022.2.1.2.Peer-Reviewed Original ResearchProcess of translatingLinguistic variationLanguage translationCross-cultural adaptationCultural nuancesLanguage barriersTranslation biasMultiple translatorsTranslation processCultural adaptationIndependent translatorsTranslatorsSpanishTranslationLatino populationEnglishTranslatingCulturallyExtensive literature reviewNuancesComprehensionLiterature reviewSeparate versionsCognitive interviewsMore attention
2021
Noncoding RNAs: biology and applications—a Keystone Symposia report
Cable J, Heard E, Hirose T, Prasanth KV, Chen L, Henninger JE, Quinodoz SA, Spector DL, Diermeier SD, Porman AM, Kumar D, Feinberg MW, Shen X, Unfried JP, Johnson R, Chen C, Wilusz JE, Lempradl A, McGeary SE, Wahba L, Pyle AM, Hargrove AE, Simon MD, Marcia M, Przanowska RK, Chang HY, Jaffrey SR, Contreras LM, Chen Q, Shi J, Mendell JT, He L, Song E, Rinn JL, Lalwani MK, Kalem MC, Chuong EB, Maquat LE, Liu X. Noncoding RNAs: biology and applications—a Keystone Symposia report. Annals Of The New York Academy Of Sciences 2021, 1506: 118-141. PMID: 34791665, PMCID: PMC9808899, DOI: 10.1111/nyas.14713.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsKeystone Symposia reportPotential drug targetsRNA biologyHuman transcriptomeEpigenetic modificationsKeystone eSymposiumNoncoding RNAsCell signalingBasic biologyDrug targetsRNABiologyDisease mechanismsNucleotidesSpeciesTranscriptomeImportant roleRNAsTranscriptionSymposium reportSignalingTranslationRoleTargetDynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo
Vinter DJ, Hoppe C, Minchington TG, Sutcliffe C, Ashe HL. Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. Development 2021, 148: dev196121. PMID: 33722899, PMCID: PMC8077512, DOI: 10.1242/dev.196121.Peer-Reviewed Original ResearchConceptsSingle mRNA resolutionDrosophila embryosHb mRNAMRNA translationAnterior-posterior patterningLive embryosSingle-molecule imagingTissue culture cellsTranslational repressionDevelopmental patterningSpatiotemporal regulationTranslational regulationExpression domainsSunTag systemTranscription factorsExpression boundariesTranslation dynamicsEmbryosCulture cellsMRNARepressionRegulationPatterningDrosophilaTranslationGenetic contributions to anxiety disorders: where we are and where we are heading
Ask H, Cheesman R, Jami ES, Levey DF, Purves KL, Weber H. Genetic contributions to anxiety disorders: where we are and where we are heading. Psychological Medicine 2021, 51: 2231-2246. PMID: 33557968, DOI: 10.1017/s0033291720005486.Peer-Reviewed Original ResearchConceptsLarge-scale genome-wide association studiesGenome-wide association studiesGenetics of anxietyGenome-wide studiesRelated traitsAssociation studiesGeneticsGenetic contributionSpecific gene-environment interactionsGene-environment interactionsCurrent knowledgeTraitsNew therapeuticsSuch discoveriesDiscoveryNew research possibilitiesLifespanTherapeuticsCircuit mechanismsTranslationTranslation and Cross-Cultural Adaptation of the Family Accommodation Scale for Obsessive-Compulsive Disorder Into Chinese.
Liao Z, You C, Chen Y, Yan L, Zhang J, Li F, Calvocoressi L, Ding L. Translation and Cross-Cultural Adaptation of the Family Accommodation Scale for Obsessive-Compulsive Disorder Into Chinese. Journal Of Cognitive Psychotherapy 2021, 35: 41-52. PMID: 33397786, DOI: 10.1891/jcpsy-d-20-00020.Peer-Reviewed Original ResearchCross-cultural adaptationOriginal English scaleLanguage adjustmentEnglish scaleFamily Accommodation ScaleConceptual equivalenceEnglish versionCultural adaptationPilot administrationChinese scaleChinese versionTranslationSteps of translationDifferent education levelsMinor editsChineseAccommodation ScalePretest stageVersionDifferent socioeconomic statusAccommodationPeopleEditsAdaptationFamily members
2020
Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma b rucei
Rajan KS, Doniger T, Cohen-Chalamish S, Rengaraj P, Galili B, Aryal S, Unger R, Tschudi C, Michaeli S. Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma b rucei. IScience 2020, 23: 101780. PMID: 33294788, PMCID: PMC7683347, DOI: 10.1016/j.isci.2020.101780.Peer-Reviewed Original ResearchRNA regulatorsProtein coding genesDistinct mRNA speciesNon-coding RNAsCoding genesPolycistronic transcriptsTranscriptional regulationMRNA translationRRNA locusMRNA functionMammalian hostsMRNA speciesMRNA stabilityGene expressionConcerted actionRegulatorParasitesRNACausative agentRegulationInsectsPolyadenylationSplicingTranslationAdditional layerEpigenetic fine-mapping: identification of causal mechanisms for autoimmunity
Lincoln MR, Axisa PP, Hafler DA. Epigenetic fine-mapping: identification of causal mechanisms for autoimmunity. Current Opinion In Immunology 2020, 67: 50-56. PMID: 32977183, DOI: 10.1016/j.coi.2020.09.002.Peer-Reviewed Original ResearchConceptsGenome-wide association studiesMolecular mechanismsSusceptibility lociIndividual susceptibility lociFundamental genetic basisCausal molecular mechanismsPathogenic cell typesSpecific molecular mechanismsGenetic susceptibility lociEpigenetic techniquesGenetic basisGenetic lociAssociation studiesCell typesLociRecent advancesMechanismGeneticsAutoimmune diseasesSpectrum of autoimmunityCausal mechanismsEtiological mechanismsInflammatory diseasesTranslationAutoimmunityCoding functions of “noncoding” RNAs
Wei LH, Guo JU. Coding functions of “noncoding” RNAs. Science 2020, 367: 1074-1075. PMID: 32139529, DOI: 10.1126/science.aba6117.Commentaries, Editorials and LettersConceptsRNA regionsProtein-coding functionProtein-coding sequencesDistinct biological processesRNA sequencing studiesLong noncoding RNAPervasive transcriptionFunctional peptidesPervasive translationHuman genomeNoncoding RNAsTranslation eventsBiological processesSequencing studiesCell growthRNATranscriptomeGenomeTranscriptionLncRNAsPeptidesMicroproteinsTranslationSubsequent studiesRegionEmerging technologies to study glial cells
Hirbec H, Déglon N, Foo LC, Goshen I, Grutzendler J, Hangen E, Kreisel T, Linck N, Muffat J, Regio S, Rion S, Escartin C. Emerging technologies to study glial cells. Glia 2020, 68: 1692-1728. PMID: 31958188, DOI: 10.1002/glia.23780.Peer-Reviewed Original ResearchConceptsCell typesChallenging biological questionsGlial cellsSpecific cell typesDifferent glial cell typesGlial cell typesBiological questionsPhysiological functionsPrecursor cellsTight interactionOligodendrocyte precursor cellsCellsExperimental approachRelative contributionBrain functionFull understandingSpecific brain functionsRoleFunctionInteractionDevelopmentTranslationChapter 68 Lung tissue engineering
Raredon M, Yuan Y, Niklason L. Chapter 68 Lung tissue engineering. 2020, 1273-1285. DOI: 10.1016/b978-0-12-818422-6.00070-8.ChaptersTissue homeostasisTissue-engineered lungsExtracellular matrixHomeostatic levelsPulmonary regenerationCell populationsGas exchangeRegeneration of lungTissue unitsFunctional unitsPrecise mixtureRegenerationTissue engineeringProteinHomeostasisBarrier integrityCellsLung tissue engineeringTissue damageTranslationBroad overview
2019
Chromosome Territorial Organization Drives Efficient Protein Complex Formation: A Hypothesis.
Bera M, Kalyana Sundaram RV. Chromosome Territorial Organization Drives Efficient Protein Complex Formation: A Hypothesis. The Yale Journal Of Biology And Medicine 2019, 92: 541-548. PMID: 31543715, PMCID: PMC6747946.Peer-Reviewed Original ResearchConceptsProtein complex assemblyProtein complex formationNascent protein chainNuclear basketExport factorsProtein complexesComplex assemblyNuclear granulesKissing loopProtein chainsEukaryotesMRNAComplex formationComplex formation efficiencyChromosomesERPathwayAssemblyLoopInterphaseComplexesGranulesTranslationFormationExport22THE PSYCHIATRIC RISK GENE NT5C2 REGULATES PROTEIN TRANSLATION IN HUMAN NEURAL PROGENITOR CELLS, AND IS INVOLVED IN LOCOMOTOR BEHAVIOUR IN DROSOPHILA MELANOGASTER
Duarte R, Bachtel N, Eleftherianos I, Nixon D, Murray R, Bray N, Powell T, Srivastava D. 22THE PSYCHIATRIC RISK GENE NT5C2 REGULATES PROTEIN TRANSLATION IN HUMAN NEURAL PROGENITOR CELLS, AND IS INVOLVED IN LOCOMOTOR BEHAVIOUR IN DROSOPHILA MELANOGASTER. European Neuropsychopharmacology 2019, 29: s1078. DOI: 10.1016/j.euroneuro.2018.08.029.Peer-Reviewed Original Research
2018
The translation of non-canonical open reading frames controls mucosal immunity
Jackson R, Kroehling L, Khitun A, Bailis W, Jarret A, York AG, Khan OM, Brewer JR, Skadow MH, Duizer C, Harman CCD, Chang L, Bielecki P, Solis AG, Steach HR, Slavoff S, Flavell RA. The translation of non-canonical open reading frames controls mucosal immunity. Nature 2018, 564: 434-438. PMID: 30542152, PMCID: PMC6939389, DOI: 10.1038/s41586-018-0794-7.Peer-Reviewed Original ResearchConceptsNon-canonical open reading framesOpen reading frameProtein-coding genomeReading frameNew open reading framesProtein-coding genesNon-protein codingNon-coding RNAsUnbiased transcriptomics approachTranscriptomic approachMethionine codonDistinct proteinsFunctional proteinsImportant transcriptsEssential roleGenomeAbsolute requirementGenesRNABacterial infectionsProteinaceous productsProteinTranslationMisannotationsCodon
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