2025
Engineering a genomically recoded organism with one stop codon
Grome M, Nguyen M, Moonan D, Mohler K, Gurara K, Wang S, Hemez C, Stenton B, Cao Y, Radford F, Kornaj M, Patel J, Prome M, Rogulina S, Sozanski D, Tordoff J, Rinehart J, Isaacs F. Engineering a genomically recoded organism with one stop codon. Nature 2025, 639: 512-521. PMID: 39910296, PMCID: PMC11903333, DOI: 10.1038/s41586-024-08501-x.Peer-Reviewed Original Research
2015
Aldehyde Capture Ligation for Synthesis of Native Peptide Bonds
Raj M, Wu H, Blosser S, Vittoria M, Arora P. Aldehyde Capture Ligation for Synthesis of Native Peptide Bonds. Journal Of The American Chemical Society 2015, 137: 6932-6940. PMID: 25966041, DOI: 10.1021/jacs.5b03538.Peer-Reviewed Original ResearchConceptsBond formationAmide bond formationNative peptide bondChemoselective reactivityChemoselective reactionAmine terminusIntramolecular reactionSynthetic proteinsPeptide bondReactionAmino acid residuesBioconjugatesFormationAcid residuesAldehydesCarboxylBondsAminesSynthesisReactivityFragmentsPeptidesResidues
2004
Functional Characterization and NMR Spectroscopy on Full-Length Vpu from HIV-1 Prepared by Total Chemical Synthesis
Kochendoerfer G, Jones D, Lee S, Oblatt-Montal M, Opella S, Montal M. Functional Characterization and NMR Spectroscopy on Full-Length Vpu from HIV-1 Prepared by Total Chemical Synthesis. Journal Of The American Chemical Society 2004, 126: 2439-2446. PMID: 14982452, DOI: 10.1021/ja038985i.Peer-Reviewed Original ResearchConceptsTotal chemical synthesisChemical synthesisNMR spectroscopyNative chemical ligation methodologyMembrane proteinsSolid-phase peptide synthesisSolid-state NMR spectraRecombinant proteinsPhase peptide synthesisSolution NMR spectroscopyFull-length VpuIntegral membrane proteinsHydrated lipid bilayerHomogeneous membrane proteinsLigation methodologyChemical ligationPeptide synthesisNMR spectraBacterial expressionFunctional characterizationSynthetic proteinsLipid micellesLipid bilayersCharacterization studiesOverall topology
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