2025
Molecular Components of Vesicle Cycling at the Rod Photoreceptor Ribbon Synapse
Hanke-Gogokhia C, Zapadka T, Finkelstein S, Arshavsky V, Demb J. Molecular Components of Vesicle Cycling at the Rod Photoreceptor Ribbon Synapse. Advances In Experimental Medicine And Biology 2025, 1468: 325-330. PMID: 39930217, DOI: 10.1007/978-3-031-76550-6_54.Peer-Reviewed Original ResearchConceptsSynaptic vesicle exocytosisSynaptic vesicle recyclingPhotoreceptor ribbon synapseVesicle exocytosisVesicle recyclingVesicle cycleVesicle releaseRibbon synapseProtein synthesisProperties of synaptic transmissionMolecular componentsMouse rodsSynaptic terminalsRod cellsProteinVesiclesRod photoreceptorsDim lightSynaptic transmissionInner segmentsCellsExocytosisEndocytosisOuter segmentsEnergy production
2024
The Structural and Functional Integrity of Rod Photoreceptor Ribbon Synapses Depends on Redundant Actions of Dynamins 1 and 3
Hanke-Gogokhia C, Zapadka T, Finkelstein S, Klingeborn M, Maugel T, Singer J, Arshavsky V, Demb J. The Structural and Functional Integrity of Rod Photoreceptor Ribbon Synapses Depends on Redundant Actions of Dynamins 1 and 3. Journal Of Neuroscience 2024, 44: e1379232024. PMID: 38641407, PMCID: PMC11209669, DOI: 10.1523/jneurosci.1379-23.2024.Peer-Reviewed Original ResearchRod ribbon synapsesDynamin-1Photoreceptor ribbon synapsesDynamin isoformsMembrane scissionRibbon synapsesConventional synapsesReduced synaptic vesicle densitySynaptic vesicle recycling processesEndocytosis of synaptic vesiclesRibbon-type active zonesVesicle densityConditional gene knockout approachGene knockout approachSynaptic vesicle densityVesicle endocytosisVesicle cycleDisrupt endocytosisDynaminSpecialized proteinsSynapse integrityEnlarged vesiclesSynaptic vesiclesKnockout approachRod photoreceptors
2020
Connectomic analysis reveals an interneuron with an integral role in the retinal circuit for night vision
Park SJ, Lieberman EE, Ke JB, Rho N, Ghorbani P, Rahmani P, Jun NY, Lee HL, Kim IJ, Briggman KL, Demb JB, Singer JH. Connectomic analysis reveals an interneuron with an integral role in the retinal circuit for night vision. ELife 2020, 9: e56077. PMID: 32412412, PMCID: PMC7228767, DOI: 10.7554/elife.56077.Peer-Reviewed Original ResearchConceptsAII amacrine cellsProjection neuronsSingle interneuron typeConnectomic analysisBipolar cell pathwaysNovel neural circuitGABAergic cellsAmacrine cellsCentral neuronsGanglion cellsSynaptic layersRetinal circuitsInterneuron typesSurround inhibitionMouse retinaNight visionON responseExcitatory centerOptogenetic analysesNeural circuitsInhibitory surroundCell pathwaysRod photoreceptorsReceptive fieldsRb pathway
2018
Restoration of vision after de novo genesis of rod photoreceptors in mammalian retinas
Yao K, Qiu S, Wang YV, Park SJH, Mohns EJ, Mehta B, Liu X, Chang B, Zenisek D, Crair MC, Demb JB, Chen B. Restoration of vision after de novo genesis of rod photoreceptors in mammalian retinas. Nature 2018, 560: 484-488. PMID: 30111842, PMCID: PMC6107416, DOI: 10.1038/s41586-018-0425-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsbeta CateninBlindnessCell CycleCell ProliferationCellular ReprogrammingDisease Models, AnimalFemaleGTP-Binding Protein alpha SubunitsHeterotrimeric GTP-Binding ProteinsMaleMiceNeurogenesisNeurogliaRegenerative MedicineRetinal Rod Photoreceptor CellsStem CellsTranscription FactorsTransducinVisual CortexVisual PathwaysConceptsMüller gliaGene transferMG proliferationRod photoreceptorsMammalian retinaCell fate specificationPopulations of stemSubsequent gene transferFate specificationRetinal stem cellsTranscription factorsRetinal neuronsCell cycleDouble mutant miceRegenerative machineryDe novo genesisΒ-cateninStem cellsProgenitor cellsRestoration of visionPrimary visual cortexMutant miceAbsence of injuryPhotoreceptorsRetinal injury
2015
A short N-terminal domain of HDAC4 preserves photoreceptors and restores visual function in retinitis pigmentosa
Guo X, Wang SB, Xu H, Ribic A, Mohns EJ, Zhou Y, Zhu X, Biederer T, Crair MC, Chen B. A short N-terminal domain of HDAC4 preserves photoreceptors and restores visual function in retinitis pigmentosa. Nature Communications 2015, 6: 8005. PMID: 26272629, PMCID: PMC4538705, DOI: 10.1038/ncomms9005.Peer-Reviewed Original ResearchConceptsRetinitis pigmentosaVisual functionRd1 miceCone photoreceptor deathMultiple cell death pathwaysRd1 mutationPhotoreceptor protectionPhotoreceptor deathEffective treatmentAnimal modelsPhotoreceptor degenerationRod deathCone photoreceptorsRod survivalInvaluable animal modelHDAC4 proteinMicePigmentosaCell death pathwaysRod photoreceptorsProtein therapyTherapyHDAC4DeathSurvival
2013
Adaptation to Background Light Enables Contrast Coding at Rod Bipolar Cell Synapses
Ke JB, Wang YV, Borghuis BG, Cembrowski MS, Riecke H, Kath WL, Demb JB, Singer JH. Adaptation to Background Light Enables Contrast Coding at Rod Bipolar Cell Synapses. Neuron 2013, 81: 388-401. PMID: 24373883, PMCID: PMC4267681, DOI: 10.1016/j.neuron.2013.10.054.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerateAdaptation, OcularAnimalsBiophysicsComputer SimulationExcitatory Amino Acid AntagonistsGlucosamine 6-Phosphate N-AcetyltransferaseGreen Fluorescent ProteinsIn Vitro TechniquesLightMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicModels, NeurologicalPatch-Clamp TechniquesQuinoxalinesRetinal Bipolar CellsRetinal Rod Photoreceptor CellsSynapsesVisual Pathways
2000
The subcellular localization of OTX2 is cell-type specific and developmentally regulated in the mouse retina
Baas D, Bumsted KM, Martinez JA, Vaccarino FM, Wikler KC, Barnstable CJ. The subcellular localization of OTX2 is cell-type specific and developmentally regulated in the mouse retina. Brain Research 2000, 78: 26-37. PMID: 10891582, DOI: 10.1016/s0169-328x(00)00060-7.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsAntibodiesBlotting, WesternCell NucleusCytoplasmGene Expression Regulation, DevelopmentalHomeodomain ProteinsHumansMiceMice, Inbred StrainsNerve Tissue ProteinsOtx Transcription FactorsPC12 CellsPigment Epithelium of EyeRabbitsRatsRetinal Ganglion CellsRetinal Rod Photoreceptor CellsTeratocarcinomaTrans-ActivatorsTransfectionTumor Cells, CulturedConceptsSubcellular localizationTranscription factorsHomeodomain-containing proteinCell fate determinationHomeodomain transcription factorCytoplasm of rodsFate determinationCell fateOtx2 proteinSubcellular distributionOtx2Retinal pigment epithelial cellsCell typesRod photoreceptorsPigment epithelial cellsRetinal developmentCytoplasmCell linesAdult eyesEpithelial cellsCentral nervous systemImmature rodsProteinCellsDifferential distribution
1997
Differentiation and Transdifferentiation of the Retinal Pigment Epithelium
Zhao S, Rizzolo L, Barnstable C. Differentiation and Transdifferentiation of the Retinal Pigment Epithelium. International Review Of Cytology 1997, 171: 225-266. PMID: 9066129, DOI: 10.1016/s0074-7696(08)62589-9.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumCell type-specific moleculesDifferent expression patternsGrowth factorLoss of polarityTranscription factorsEmbryonic developmentLoss of expressionRetinal cell typesExpression patternsPrE cellsFibroblast growth factorBasic fibroblast growth factorPigment epitheliumCell typesRod photoreceptorsUnique moleculesOcular metabolismRPE layerMonolayer culturesWide arrayExpressionCellsMammalsMolecules
1996
Phospholipase C β4 is involved in modulating the visual response in mice
Jiang H, Lyubarsky A, Dodd R, Vardi N, Pugh E, Baylor D, Simon M, Wu D. Phospholipase C β4 is involved in modulating the visual response in mice. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 14598-14601. PMID: 8962098, PMCID: PMC26179, DOI: 10.1073/pnas.93.25.14598.Peer-Reviewed Original ResearchConceptsPLC-beta 4Beta 4Phospholipase C beta 4Cell-cell interactionsC beta 4Phototransduction cascadePLC-betaRod photoreceptorsPhysiological significanceMouse linesLateral geniculate nucleusPhospholipase C β4Wild-type littermatesElectroretinographic resultsVisual functionGeniculate nucleusLittermate controlsSuperior colliculusRod aRetinal cellsBehavioral testsLight microscopyB-wave componentVisual responsesMiceSpatiotemporal gradients of cell genesis in the primate retina.
Rapaport D, Rakic P, LaVail M. Spatiotemporal gradients of cell genesis in the primate retina. Perspectives On Developmental Neurobiology 1996, 3: 147-59. PMID: 8931090.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsGanglion cellsCell genesisMonkey retinal ganglion cellsLonger survival periodRetinal pigment epitheliumAmacrine cellsPrimate retinaMüller cellsSurvival periodMacaque retinaSoma diameterBipolar cellsPigment epitheliumRetinal structureHorizontal cellsMacaque monkeysVisual developmentCone photoreceptorsBrain structuresFunctional classRetinaProgenitor cellsRod photoreceptorsCardinal event
1994
Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors
Ahmad I, Leinders-Zufall T, Kocsis J, Shepherd G, Zufall F, Barnstable C. Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors. Neuron 1994, 12: 155-165. PMID: 7507337, DOI: 10.1016/0896-6273(94)90160-0.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine8-Bromo Cyclic Adenosine MonophosphateAnimalsAnimals, NewbornBase SequenceCells, CulturedCyclic GMPCyclic Nucleotide-Gated Cation ChannelsCysteineDNA PrimersElectric ConductivityGene ExpressionIn Situ HybridizationIon ChannelsMembrane PotentialsModels, NeurologicalMolecular Sequence DataNitric OxideNitroprussidePolymerase Chain ReactionRatsRats, WistarRetinal Ganglion CellsRetinal Rod Photoreceptor CellsS-NitrosothiolsTranscription, GeneticConceptsRetinal ganglion cellsGanglion cellsWhole-cell patch-clamp recordingsRat retinal ganglion cellsNitric oxide donor sodium nitroprussideCation channelsGanglion cell activityPatch-clamp recordingsCation conductanceNitric oxide donorDonor sodium nitroprussideNonselective cation channelsAmacrine cellsSodium nitroprussideOxide donorPhosphodiesterase inhibitorCell activityPolymerase chain reaction amplificationNitric oxideChain reaction amplificationReversal potentialOutward rectificationRod photoreceptorsCGMPS-nitrosocysteine
1992
Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel.
Ahmad I, Korbmacher C, Segal A, Cheung P, Boulpaep E, Barnstable C. Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 10262-10266. PMID: 1279673, PMCID: PMC50318, DOI: 10.1073/pnas.89.21.10262.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernCell LineCyclic GMPDNAIon Channel GatingIon ChannelsKidney CortexKidney Tubules, CollectingMembrane PotentialsMiceMolecular Sequence DataOligodeoxyribonucleotidesPhotoreceptor CellsPoly APolymerase Chain ReactionRatsRNARNA, MessengerSequence Homology, Nucleic AcidConceptsNonselective cation channelsPhotoreceptor channelDuct cellsCation channel probeCation channelsChannel activityPolymerase chain reactionNonselective cation channel activityCGMP decreaseCation channel activityChannel subunitsCytoplasmic applicationChain reactionRod photoreceptorsNorthern blot analysisRetinal cGMPBlot analysisCGMPRat kidney cDNA libraryChannel genesMiceSingle-channel conductanceOpen probabilityCellsCalcium removal
1977
Electrical and adaptive properties of rod photoreceptors in Bufo marinus. II. Effects of cyclic nucleotides and prostaglandins.
Lipton S, Rasmussen H, Dowling J. Electrical and adaptive properties of rod photoreceptors in Bufo marinus. II. Effects of cyclic nucleotides and prostaglandins. Journal Of General Physiology 1977, 70: 771-791. PMID: 201724, PMCID: PMC2228513, DOI: 10.1085/jgp.70.6.771.Peer-Reviewed Original ResearchConceptsCombination of drugsIsolated toad retinaCyclic nucleotide levelsCombination of IBMXResponse amplitudeDrug levelsReceptor sensitivityLong-term exposureReceptor saturationIntracellular cGMPMM IBMXRetinaControl RingerSimilar alterationsElectrical activityCGMPRod photoreceptorsV-log IATPase pumpRod activitySuperfusionDark adaptationOuter segmentsSensitivity recoveryCyclic nucleotidesElectrical and adaptive properties of rod photoreceptors in Bufo marinus. I. Effects of altered extracellular Ca2+ levels.
Lipton S, Ostroy S, Dowling J. Electrical and adaptive properties of rod photoreceptors in Bufo marinus. I. Effects of altered extracellular Ca2+ levels. Journal Of General Physiology 1977, 70: 747-770. PMID: 412914, PMCID: PMC2228514, DOI: 10.1085/jgp.70.6.747.Peer-Reviewed Original ResearchConceptsReceptor sensitivityLight-evoked responsesResponse amplitudeLow calcium RingerMembrane potential levelMembrane potentialExtracellular Ca2Intracellular storesAdapted rodRod sensitivityReceptor potentialElectrical activityRod photoreceptorsRinger's solutionV-log IRingerDark adaptationProlonged exposureSensitivity recoveryRetinaResponse saturationSubstantial depolarizationBufo marinus
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