2025
Exploring Calcium Channels as Potential Therapeutic Targets in Blast Traumatic Brain Injury
Wachtler N, O’Brien R, Ehrlich B, McGuone D. Exploring Calcium Channels as Potential Therapeutic Targets in Blast Traumatic Brain Injury. Pharmaceuticals 2025, 18: 223. PMID: 40006037, PMCID: PMC11859800, DOI: 10.3390/ph18020223.Peer-Reviewed Original ResearchCalcium signalingCalcium channelsTherapeutic strategiesCalcium homeostasisFunction of calcium channelsDysregulated calcium signalingModulation of injuryTraumatic brain injuryBrain injuryLoss of calcium homeostasisBlast-related traumatic brain injuryDevelopment of neuroprotective interventionsIntracellular calcium dynamicsPlasma membrane stabilityExtracellular calciumBlast traumatic brain injuryPreclinical modelsTherapeutic outcomesNeuroprotective interventionsMembrane abnormalitiesPharmacological inhibitorsNeuronal somataExclusion criteriaCalcium dynamicsSecondary injury
2023
Plant MDL proteins synergize with the cytokine MIF at CXCR2 and CXCR4 receptors in human cells
Spiller L, Manjula R, Leissing F, Basquin J, Bourilhon P, Sinitski D, Brandhofer M, Levecque S, Gerra S, Sabelleck B, Zhang L, Feederle R, Flatley A, Hoffmann A, Panstruga R, Bernhagen J, Lolis E. Plant MDL proteins synergize with the cytokine MIF at CXCR2 and CXCR4 receptors in human cells. Science Signaling 2023, 16: eadg2621. PMID: 37988455, DOI: 10.1126/scisignal.adg2621.Peer-Reviewed Original ResearchConceptsMammalian macrophage migration inhibitory factorHetero-oligomeric complexesHigh structural similarityMultifunctional inflammatory cytokineHuman lung epithelial cellsYeast reporter systemReporter systemLung epithelial cellsPlant leavesFunctional similarityCellular responsesHuman cellsPharmacological inhibitorsDopachrome tautomeraseFunctional implicationsX-ray crystallographyMacrophage migration inhibitory factorStructural similarityEpithelial cellsMigration inhibitory factorCXCR4 receptorProteinTautomerase activityCellsMIF receptor
2021
Targeting OCT3 attenuates doxorubicin-induced cardiac injury
Huang KM, Thomas M, Magdy T, Eisenmann ED, Uddin ME, DiGiacomo DF, Pan A, Keiser M, Otter M, Xia SH, Li Y, Jin Y, Fu Q, Gibson AA, Bonilla IM, Carnes CA, Corps KN, Coppola V, Smith SA, Addison D, Nies AT, Bundschuh R, Chen T, Lustberg MB, Wang J, Oswald S, Campbell MJ, Yan PS, Baker SD, Hu S, Burridge PW, Sparreboom A. Targeting OCT3 attenuates doxorubicin-induced cardiac injury. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2020168118. PMID: 33495337, PMCID: PMC7865186, DOI: 10.1073/pnas.2020168118.Peer-Reviewed Original ResearchConceptsOrganic cation transporter 3Cardiac injuryCardiovascular functionSide effectsTranslational relevanceCalcium-binding proteins S100A8Irreversible cardiac injuryCurrent preventative strategiesPotential translational relevanceCardiac damagePlasma levelsCardiac accumulationBreast cancerAntitumor effectsPharmacological targetingPreventative strategiesModest protectionProteins S100A8Critical transporterTransporter 3Pharmacological inhibitorsOverexpression modelIntervention strategiesDoxorubicinCardiotoxicity
2016
Inhibition of Regulatory-Associated Protein of Mechanistic Target of Rapamycin Prevents Hyperoxia-Induced Lung Injury by Enhancing Autophagy and Reducing Apoptosis in Neonatal Mice
Sureshbabu A, Syed M, Das P, Janér C, Pryhuber G, Rahman A, Andersson S, Homer RJ, Bhandari V. Inhibition of Regulatory-Associated Protein of Mechanistic Target of Rapamycin Prevents Hyperoxia-Induced Lung Injury by Enhancing Autophagy and Reducing Apoptosis in Neonatal Mice. American Journal Of Respiratory Cell And Molecular Biology 2016, 55: 722-735. PMID: 27374190, PMCID: PMC5105179, DOI: 10.1165/rcmb.2015-0349oc.Peer-Reviewed Original ResearchMeSH KeywordsAcute Lung InjuryAdaptor Proteins, Signal TransducingAlveolar Epithelial CellsAnimalsAnimals, NewbornApoptosisAutophagyBronchopulmonary DysplasiaCell LineFemaleHumansHyperoxiaHypertension, PulmonaryHypertrophy, Right VentricularInfant, NewbornLungMiceMicrotubule-Associated ProteinsNaphthyridinesPhenotypeRegulatory-Associated Protein of mTORTime FactorsTumor Suppressor Protein p53ConceptsAcute lung injuryBronchopulmonary dysplasiaLung injuryWild-type miceMechanistic targetRegulatory-Associated ProteinLysosomal-associated membrane protein 1Apoptotic cell deathFetal type II alveolar epithelial cellsMouse lungRole of autophagyHyperoxia-Induced Lung InjuryLight chain 3Activation of autophagyType II alveolar epithelial cellsRespiratory distress syndromeMembrane protein 1Developmental lung diseaseUseful therapeutic targetNeonatal mouse lungAlveolar epithelial cellsPharmacological inhibitorsTreatment of hyperoxiaCell deathAutophagic fluxEGLN1 Inhibition and Rerouting of α-Ketoglutarate Suffice for Remote Ischemic Protection
Olenchock B, Moslehi J, Baik A, Davidson S, Williams J, Gibson W, Chakraborty A, Pierce K, Miller C, Hanse E, Kelekar A, Sullivan L, Wagers A, Clish C, Vander Heiden M, Kaelin W. EGLN1 Inhibition and Rerouting of α-Ketoglutarate Suffice for Remote Ischemic Protection. Cell 2016, 164: 884-895. PMID: 26919427, PMCID: PMC4819986, DOI: 10.1016/j.cell.2016.02.006.Peer-Reviewed Original ResearchConceptsRemote ischemic preconditioningIschemic preconditioningHypoxia-inducible factorKynurenic acidIschemic protectionMyocardial ischemia-reperfusion (I/R) injuryIschemia-reperfusion (I/R) injuryParabiosis experimentsPeriod of sublethal ischemiaMouse modelIschemic insultSecreted factorsPharmacological inhibitorsHepatic productionOther organsGene deletionSublethal ischemiaIschemiaMiceEGLN1InhibitionInhibition of Fatty Acid Synthase Decreases Expression of Stemness Markers in Glioma Stem Cells
Yasumoto Y, Miyazaki H, Vaidyan LK, Kagawa Y, Ebrahimi M, Yamamoto Y, Ogata M, Katsuyama Y, Sadahiro H, Suzuki M, Owada Y. Inhibition of Fatty Acid Synthase Decreases Expression of Stemness Markers in Glioma Stem Cells. PLOS ONE 2016, 11: e0147717. PMID: 26808816, PMCID: PMC4726602, DOI: 10.1371/journal.pone.0147717.Peer-Reviewed Original ResearchConceptsFatty acid synthaseGlioma stem cellsStem cellsCellular lipid metabolismCellular metabolic changesKey lipogenic enzymesPatient-derived glioma stem cellsLipid biosynthesisFatty acid binding proteinAcid binding proteinPharmacological inhibitorsSynthesis assayBinding proteinAcid synthaseCerulenin treatmentGlial fibrillary acidic protein (GFAP) expressionGSC stemnessProtein expressionCancer cellsDe novo lipogenesisLipogenic enzymesImmunocytochemical analysisMarker nestinStemness markersDecrease expression
2015
Early to Late Endosome Trafficking Controls Secretion and Zymogen Activation in Rodent and Human Pancreatic Acinar Cells
Messenger SW, Thomas D, Cooley MM, Jones EK, Falkowski MA, August BK, Fernandez LA, Gorelick FS, Groblewski GE. Early to Late Endosome Trafficking Controls Secretion and Zymogen Activation in Rodent and Human Pancreatic Acinar Cells. Cellular And Molecular Gastroenterology And Hepatology 2015, 1: 695-709. PMID: 26618189, PMCID: PMC4657148, DOI: 10.1016/j.jcmgh.2015.08.002.Peer-Reviewed Original ResearchEarly endosomesRecycling endosomesEndosomal traffickingLate endosomesDominant-negative Rab11a mutantCellular damage responseAssociated membrane proteinLysosome-Associated Membrane ProteinsPIKfyve inhibitionApical traffickingPIKfyve activityEndosomal systemCCK-8Damage responseMembrane proteinsPlasma membraneAcinar cellsPIKfyvePharmacological inhibitorsPancreatic acinar cellsTraffickingEndosomesZymogen activationAdenoviral overexpressionCellular deathABT‐737 Inhibits Full Length And Cleaved Pro‐Apoptotic Bcl‐xL, Resulting in Differential Effects on Death And Survival
Park H, Licznerski P, Niu Y, Alavian K, Jonas E. ABT‐737 Inhibits Full Length And Cleaved Pro‐Apoptotic Bcl‐xL, Resulting in Differential Effects on Death And Survival. The FASEB Journal 2015, 29 DOI: 10.1096/fasebj.29.1_supplement.777.4.Peer-Reviewed Original ResearchFull-length Bcl-xLBcl-xLABT-737Mitochondrial potentialATP productionΔN-BclAnti-apoptotic Bcl-2 family proteinsBcl-2 family proteinsCell death stimuliMitochondrial membrane permeabilityATP synthase activityMitochondrial permeability transition porePro-apoptotic BclPro-apoptotic formBcl-xL inhibitorsPermeability transition poreDeath stimuliFamily proteinsBcl-xL.Isolated mitochondriaPharmacological inhibitorsTransition poreCell deathFull lengthSynthase activity
2014
Sequential Application of a Cytotoxic Nanoparticle and a PI3K Inhibitor Enhances Antitumor Efficacy
Pandey A, Kulkarni A, Roy B, Goldman A, Sarangi S, Sengupta P, Phipps C, Kopparam J, Oh M, Basu S, Kohandel M, Sengupta S. Sequential Application of a Cytotoxic Nanoparticle and a PI3K Inhibitor Enhances Antitumor Efficacy. Cancer Research 2014, 74: 675-685. PMID: 24121494, PMCID: PMC3946433, DOI: 10.1158/0008-5472.can-12-3783.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsBreast NeoplasmsCell Line, TumorCell SurvivalCisplatinErbB ReceptorsFemaleHumansMiceModels, BiologicalNanoparticlesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsProtein Kinase InhibitorsProto-Oncogene Proteins c-aktReproducibility of ResultsSignal TransductionConceptsMolecular targeted therapeuticsEnhanced antitumor efficacyAntitumor efficacyBreast cancerCytotoxic agents to tumorsPharmacological inhibitor of PI3KImpact of drug sequencingCombination of nanomedicineAgents to tumorsBreast cancer cellsPI3K) pathwayManagement of cancerInhibitor of PI3KCytotoxic nanoparticlesCombination therapyDrug sequenceOncogenic driversAntitumor outcomesProsurvival signalingCancer cellsPharmacological inhibitorsCancer chemotherapyInduce apoptosisCancerIn vivo validation
2013
Contrasting effects of chronic, systemic treatment with mTOR inhibitors rapamycin and metformin on adult neural progenitors in mice
Kusne Y, Goldberg EL, Parker SS, Hapak SM, Maskaykina IY, Chew WM, Limesand KH, Brooks HL, Price TJ, Sanai N, Nikolich-Zugich J, Ghosh S. Contrasting effects of chronic, systemic treatment with mTOR inhibitors rapamycin and metformin on adult neural progenitors in mice. GeroScience 2013, 36: 199-212. PMID: 23949159, PMCID: PMC3889877, DOI: 10.1007/s11357-013-9572-5.Peer-Reviewed Original ResearchConceptsSystemic administrationMTOR inhibitorsImproved health spanAdult-born neuronsHealth spanEffects of chronicNeural progenitorsAdult neural stem cellsMTOR inhibitor rapamycinInhibition of mTORPotential adverse effectsAdult neural progenitorsNeural stem cellsSystemic treatmentDendate gyrusMouse hippocampusSubventricular regionOrgan functionMetforminBehavioral healthInhibitor rapamycinAdverse effectsPharmacological inhibitorsMTORRapamycin
2012
Insulin-Like Growth Factors Promote Vasculogenesis in Embryonic Stem Cells
Piecewicz S, Pandey A, Roy B, Xiang S, Zetter B, Sengupta S. Insulin-Like Growth Factors Promote Vasculogenesis in Embryonic Stem Cells. PLOS ONE 2012, 7: e32191. PMID: 22363814, PMCID: PMC3283730, DOI: 10.1371/journal.pone.0032191.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCell DifferentiationEmbryoid BodiesEmbryonic Stem CellsEndotheliumExtracellular Signal-Regulated MAP KinasesHypoxia-Inducible Factor 1, alpha SubunitInsulin-Like Growth Factor IInsulin-Like Growth Factor IIMesodermMiceModels, BiologicalNeovascularization, PhysiologicPodophyllotoxinProtein StabilityProto-Oncogene Proteins c-aktReceptor, IGF Type 1Receptor, IGF Type 2Signal TransductionVascular Endothelial Growth Factor AConceptsEmbryonic stem cellsStem cellsEndothelial differentiationInsulin-like growth factorStem cell-based modelsUp-regulation of VEGFIGF receptorsNeutralizing antibodiesFunctional blood vesselsPharmacological inhibitorsTherapeutic angiogenesisGrowth factorEndothelial cellsPromote vasculogenesisCell-based modelsCellular mechanismsGerm layersEmbryonic stemMesodermal lineagesEmbryoid body modelBlood vesselsEmbryoid bodiesVasculogenesisUp-regulatedTherapy
2011
Cholinergic signaling inhibits oxalate transport by human intestinal T84 cells
Hassan HA, Cheng M, Aronson PS. Cholinergic signaling inhibits oxalate transport by human intestinal T84 cells. American Journal Of Physiology - Cell Physiology 2011, 302: c46-c58. PMID: 21956166, PMCID: PMC3328906, DOI: 10.1152/ajpcell.00075.2011.Peer-Reviewed Original ResearchConceptsC-SrcT84 cellsHuman intestinal cell line T84PKC-δ inhibitor rottlerinSrc inhibitor PP2Intestinal cell line T84Human intestinal T84 cellsSurface expressionMuscarinic receptorsRegulation downstreamInhibitor PP2Phosphorylation studiesBiotinylation studiesInhibitor rottlerinKnockdown studiesPharmacological inhibitorsIntestinal T84 cellsIntestinal oxalate secretionPhospholipase CIntestinal ion transportPKC activationSignificant translocationCalcium oxalate urolithiasisPKCAnion exchanger SLC26A6
2010
Dlxin-1, a MAGE family protein, induces accelerated neurite outgrowth and cell survival by enhanced and early activation of MEK and Akt signalling pathways in PC12 cells
Reddy E, Chettiar S, Kaur N, Shepal V, Shiras A. Dlxin-1, a MAGE family protein, induces accelerated neurite outgrowth and cell survival by enhanced and early activation of MEK and Akt signalling pathways in PC12 cells. Experimental Cell Research 2010, 316: 2220-2236. PMID: 20595047, DOI: 10.1016/j.yexcr.2010.05.030.Peer-Reviewed Original ResearchConceptsDlxin-1MAGE homology domainCell survivalPC12 cellsPresence of NGFNeuronal differentiationDiverse cellular functionsMAGE family proteinsCell cycle progressionTranscriptional regulationHomology domainCellular functionsFamily proteinsNeuronal survivalDevelopmental apoptosisEnhanced neuritogenesisCycle progressionSignaling pathwaysMEK pathwayPharmacological inhibitorsCell deathAkt pathwayUnique regionAmino acidsEarly activation
2009
Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death
Choy JC, Pober JS. Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death. American Journal Of Transplantation 2009, 9: 2281-2291. PMID: 19663890, PMCID: PMC3505447, DOI: 10.1111/j.1600-6143.2009.02771.x.Peer-Reviewed Original ResearchConceptsHuman CD8 T cellsCD8 T cellsInducible NO synthaseT cellsActivated T cellsNitric oxideDeprivation-induced cell deathCell deathAllogeneic endothelial cellsT cell proliferationActivation-induced cell deathCytokine deprivationGeneration of NOT cell deathExogenous nitric oxideHuman T cellsNO augmentsAllogeneic responseS-nitrosylationNO synthaseProtective effectActivity of caspasesEndothelial cellsPharmacological inhibitorsDeathParacrine Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death (141.3)
Choy J, Pober J. Paracrine Generation of NO by Bystander Human CD8 T Cells Augments Allogeneic Responses by Inhibiting Cytokine Deprivation-Induced Cell Death (141.3). The Journal Of Immunology 2009, 182: 141.3-141.3. DOI: 10.4049/jimmunol.182.supp.141.3.Peer-Reviewed Original ResearchHuman CD8 T cellsCD8 T cellsT cellsActivated T cellsDeprivation-induced cell deathCell deathAllogeneic endothelial cellsT cell proliferationActivation-induced cell deathCytokine deprivationHuman T cellsProtein expression levelsNO augmentsAllogeneic responseS-nitrosylationProtective effectExogenous NOActivity of caspasesEndothelial cellsPharmacological inhibitorsDeathBcl-2Cell proliferationExpression levelsINOS
2007
SLC26A7 Can function as a chloride-loading mechanism in parietal cells
Kosiek O, Busque SM, Föller M, Shcheynikov N, Kirchhoff P, Bleich M, Muallem S, Geibel JP. SLC26A7 Can function as a chloride-loading mechanism in parietal cells. Pflügers Archiv - European Journal Of Physiology 2007, 454: 989-998. PMID: 17404755, DOI: 10.1007/s00424-007-0254-y.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsAntiportersChloride-Bicarbonate AntiportersChloridesFluorescent Antibody TechniqueGastric AcidGastric MucosaImage Processing, Computer-AssistedIn Vitro TechniquesMaleOocytesParietal Cells, GastricPatch-Clamp TechniquesRatsRats, Sprague-DawleySodiumSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2Sulfate TransportersXenopusConceptsParietal cellsXenopus laevis oocyte expression systemRat parietal cellsExpression of SLC26A7Rat gastric glandsAcid secretionOocyte expression systemNKCC inhibitorWestern blotHistamine stimulationPharmacological inhibitorsAcidic pHiGastric glandsDIDS concentrationExchanger AE2Subsequent readditionTransporter activityDisulfonic acidInhibitorsCellsSLC26A7InfluxImmunohistochemistrySecretagoguesUptake system
2006
Scaffolding Protein Grb2-associated Binder 1 Sustains Epidermal Growth Factor-induced Mitogenic and Survival Signaling by Multiple Positive Feedback Loops*
Kiyatkin A, Aksamitiene E, Markevich NI, Borisov NM, Hoek JB, Kholodenko BN. Scaffolding Protein Grb2-associated Binder 1 Sustains Epidermal Growth Factor-induced Mitogenic and Survival Signaling by Multiple Positive Feedback Loops*. Journal Of Biological Chemistry 2006, 281: 19925-19938. PMID: 16687399, PMCID: PMC2312093, DOI: 10.1074/jbc.m600482200.Peer-Reviewed Original ResearchConceptsEpidermal growth factorRas/MAPK signalingGab1 tyrosine phosphorylationGrowth factorRole of Gab1PI3K/Akt activationMultiple positive feedback loopsProtein Grb2Mutant proteinsScaffold proteinTyrosine phosphorylationBinder 1Positive feedback loopMitogenic pathwaysMAPK signalingEssential functionsSurvival signalingDiverse perturbationsCellular responsesAkt activationCytokine receptorsPharmacological inhibitorsGab1EGF dosesGrb2
2001
Gα16 Couples Chemoattractant Receptors to NF-κB Activation
Yang M, Sang H, Rahman A, Wu D, Malik A, Ye R. Gα16 Couples Chemoattractant Receptors to NF-κB Activation. The Journal Of Immunology 2001, 166: 6885-6892. PMID: 11359849, DOI: 10.4049/jimmunol.166.11.6885.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAmino Acid SubstitutionCell NucleusEnzyme ActivationGenes, ReporterGlutamineGTP-Binding Protein alpha Subunits, Gq-G11HeLa CellsHeterotrimeric GTP-Binding ProteinsHumansIsoenzymesLeucineLuciferasesNF-kappa BPhospholipase C betaReceptors, ChemokineReceptors, Formyl PeptideReceptors, ImmunologicReceptors, PeptideSignal TransductionTrans-ActivatorsTransfectionType C PhospholipasesConceptsNF-kappaB activationFormyl peptide receptorChemoattractant receptorsPeptide receptorCXC chemokine receptor 2NF-kappaB.Chemokine receptor 2Regulatory protein alpha subunitNF-κB activationLuciferase activityProtein kinase CKappaB-luciferase activityPhosphorylation of IkappaBalphaPhospholipase C-beta activityC3a receptorReceptor 2Seven-membrane-spanning receptorsAgonist stimulationConventional PKC isoformsPharmacological inhibitorsLuciferase reporterReceptorsHemopoietic cellsProtein alpha subunitsCell model system
1997
Efficient Scavenging of Fatty Acid Oxidation Products by Aminoguanidine
Al-Abed Y, Bucala R. Efficient Scavenging of Fatty Acid Oxidation Products by Aminoguanidine. Chemical Research In Toxicology 1997, 10: 875-879. PMID: 9282836, DOI: 10.1021/tx970035l.Peer-Reviewed Original ResearchConceptsLow-density lipoprotein levelsAdvanced glycation end productsGlycation end productsMechanism of actionVascular wall componentsLipoprotein levelsLipoprotein metabolismOxidative stressPharmacological inhibitorsFatty acid oxidation productsHuman subjectsToxic effectsAminoguanidineOxidative damageNormal pathwayReactive aldehydesRecent studiesPotential interactionsDamaging effectsVivoTissue constituentsEnd productsAtherogenesisLipid oxidationMalondialdehyde
1995
Physiological and pharmacological inhibitors of luteinizing hormone-dependent steroidogenesis induce heat shock protein-70 in rat luteal cells
Khanna A, Aten R, Behrman H. Physiological and pharmacological inhibitors of luteinizing hormone-dependent steroidogenesis induce heat shock protein-70 in rat luteal cells. Endocrinology 1995, 136: 1775-1781. DOI: 10.1210/en.136.4.1775.Peer-Reviewed Original ResearchRat luteal cellsInduction of HSP-70Inducible HSP-70Luteal cellsHSP-70Progesterone synthesisHeat shock protein inductionPharmacological inhibitorsHeat shock transcriptional control elementTumor necrosis factor-alphaInhibition of progesterone synthesisHeat shock proteinsLuteal regressionPhorbol 12-myristate 13-acetateNecrosis factor-alphaProstaglandin F2 alphaAssociated with inhibitionFunctional luteal regressionCAMP accumulationFactor-alphaF2 alphaPGF2 alphaAlpha treatmentPGF2 alpha treatmentHeat shock protein 70
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