2025
Optic nerve injury impairs intrinsic mechanisms underlying electrical activity in a resilient retinal ganglion cell
Zapadka T, Tran N, Demb J. Optic nerve injury impairs intrinsic mechanisms underlying electrical activity in a resilient retinal ganglion cell. The Journal Of Physiology 2025 PMID: 39985791, DOI: 10.1113/jp286414.Peer-Reviewed Original ResearchOptic nerve crushRetinal ganglion cellsOptic nerveGanglion cellsSynaptic inputsVoltage-gated sodium channel currentsRetinal ganglion cell typesVoltage-gatedRetinal ganglion cell survivalChelation of intracellular calciumResting membrane potentialOptic nerve injuryVoltage-gated currentsAxonal injurySodium channel currentsRetinal ganglion cell axonsRGC typesAlpha retinal ganglion cellsAxon initial segmentIntracellular calciumRate of survivalNerve injuryElectrophysiological propertiesNerve crushIntrinsic excitability
2022
Rabphilin3A reduces integrin-dependent growth cone signaling to restrict axon regeneration after trauma
Sekine Y, Kannan R, Wang X, Strittmatter SM. Rabphilin3A reduces integrin-dependent growth cone signaling to restrict axon regeneration after trauma. Experimental Neurology 2022, 353: 114070. PMID: 35398339, PMCID: PMC9555232, DOI: 10.1016/j.expneurol.2022.114070.Peer-Reviewed Original ResearchConceptsAxon regenerationModerate spinal cord contusion injurySpinal cord contusion injuryTraumatic spinal cord injuryAdult mammalian central nervous systemGrowth conesRetinal ganglion cell axonsOptic nerve crushSpinal cord crush injuryGanglion cell axonsSpinal cord injuryMammalian central nervous systemCentral nervous systemCorticospinal axon regenerationContusion injuryAxonal sproutingCrush injuryNerve crushAxonal growth conesCord injuryAxon sproutingCell axonsProximal bodyNervous systemNeural repair
2021
Optic nerve regeneration screen identifies multiple genes restricting adult neural repair
Lindborg JA, Tran NM, Chenette DM, DeLuca K, Foli Y, Kannan R, Sekine Y, Wang X, Wollan M, Kim IJ, Sanes JR, Strittmatter SM. Optic nerve regeneration screen identifies multiple genes restricting adult neural repair. Cell Reports 2021, 34: 108777. PMID: 33657370, PMCID: PMC8009559, DOI: 10.1016/j.celrep.2021.108777.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsCRISPR-Cas SystemsDependovirusFemaleGene EditingGene Expression RegulationGenetic Association StudiesHEK293 CellsHumansInterleukinsMaleMAP Kinase Kinase KinasesMice, Inbred C57BLMice, TransgenicNerve RegenerationNeurogenesisOptic NerveOptic Nerve InjuriesRetinal Ganglion CellsSignal TransductionSTAT3 Transcription FactorConceptsOptic nerve crushRetinal ganglion cellsRegeneration-associated genesShort hairpin RNAIL-22Neural repairCentral nervous system traumaNeurological deficits persistNervous system traumaNerve crushAxonal damageAxonal regenerationGanglion cellsSystem traumaInflammatory responseCNS regenerationDeficits persistAxonal growthHairpin RNAConcurrent activationTranscription 3Cell-autonomous factorsKinase pathwaySignal transducerRepair
2018
Functional Genome-wide Screen Identifies Pathways Restricting Central Nervous System Axonal Regeneration
Sekine Y, Lin-Moore A, Chenette DM, Wang X, Jiang Z, Cafferty WB, Hammarlund M, Strittmatter SM. Functional Genome-wide Screen Identifies Pathways Restricting Central Nervous System Axonal Regeneration. Cell Reports 2018, 23: 415-428. PMID: 29642001, PMCID: PMC5937716, DOI: 10.1016/j.celrep.2018.03.058.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsCaenorhabditis elegansCaenorhabditis elegans ProteinsCentral Nervous SystemFemaleGene Regulatory NetworksGenomeMiceMice, Inbred C57BLMice, KnockoutNerve RegenerationOptic Nerverab GTP-Binding ProteinsRecovery of FunctionRetinal Ganglion CellsRNA InterferenceRNA, Small InterferingSpinal Cord InjuriesSuppressor of Cytokine Signaling ProteinsConceptsAxonal regenerationCentral nervous system axonal regenerationRetinal ganglion cell axon regenerationGreater motor functionOptic nerve crushCerebral cortical neuronsSpinal cord traumaNeurological recoveryCord traumaNerve crushCNS injuryAxonal regrowthCortical neuronsMotor functionAxon regenerationReceptor bindingComprehensive functional screenAdult mammalsInjuryMultiple pathwaysExpression profilesIdentifies pathwaysSignificant overlapPathwayFunction screen
2017
Regulation of axonal regeneration by the level of function of the endogenous Nogo receptor antagonist LOTUS
Hirokawa T, Zou Y, Kurihara Y, Jiang Z, Sakakibara Y, Ito H, Funakoshi K, Kawahara N, Goshima Y, Strittmatter SM, Takei K. Regulation of axonal regeneration by the level of function of the endogenous Nogo receptor antagonist LOTUS. Scientific Reports 2017, 7: 12119. PMID: 28935984, PMCID: PMC5608707, DOI: 10.1038/s41598-017-12449-6.Peer-Reviewed Original ResearchConceptsSpinal cord injuryOptic nerve crushAxonal regenerationMotor recoveryNerve crushNeural repairRetinal ganglion cell axonal regenerationAdult mammalian central nervous systemIntrinsic motor recoverySpontaneous neural repairAxonal growth inhibitorsMammalian central nervous systemCentral nervous systemNon-permissive environmentLevel of functionUntreated miceFunctional recoveryCord injuryReceptor antagonistNeuronal overexpressionNervous systemGenetic deletionViral overexpressionCrushInhibitors
2015
Intravitreal Delivery of Human NgR-Fc Decoy Protein Regenerates Axons After Optic Nerve Crush and Protects Ganglion Cells in Glaucoma ModelsNgR-Fc Rescues Ganglion Cells in Glaucoma
Wang X, Lin J, Arzeno A, Choi JY, Boccio J, Frieden E, Bhargava A, Maynard G, Tsai JC, Strittmatter SM. Intravitreal Delivery of Human NgR-Fc Decoy Protein Regenerates Axons After Optic Nerve Crush and Protects Ganglion Cells in Glaucoma ModelsNgR-Fc Rescues Ganglion Cells in Glaucoma. Investigative Ophthalmology & Visual Science 2015, 56: 1357-1366. PMID: 25655801, PMCID: PMC4338631, DOI: 10.1167/iovs.14-15472.Peer-Reviewed Original ResearchConceptsOptic nerve crushFluro-GoldNerve crushAxonal regenerationGanglion cellsOptic nerve crush injuryRetinal ganglion cell degenerationRGC axonal regenerationNerve crush injuryDisease-modifying therapiesGanglion cell degenerationDecoy proteinMicrobead modelVitreal spaceIntravitreal treatmentRGC densityAxonal sproutingCrush injuryGlaucoma modelNeuroprotective effectsAnterior chamberControl ratsVision lossAnterograde labelingBolus administration
2013
Sciatic nerve regeneration is not inhibited by anti-NGF antibody treatment in the adult rat
Lankford K, Arroyo E, Liu C, Somps C, Zorbas M, Shelton D, Evans M, Hurst S, Kocsis J. Sciatic nerve regeneration is not inhibited by anti-NGF antibody treatment in the adult rat. Neuroscience 2013, 241: 157-169. PMID: 23531437, DOI: 10.1016/j.neuroscience.2013.03.024.Peer-Reviewed Original ResearchConceptsNerve growth factorAdult ratsNerve regenerationFunctional recoveryAnti-NGF antibody treatmentElevated nerve growth factorUnilateral sciatic nerve crushDorsal root ganglion neuronsAnti-NGF antibodySciatic nerve crushType of painVehicle-treated animalsSciatic nerve regenerationPost nerve injuryNovel therapeutic approachesCell body sizePeripheral nerve regenerationFluro-GoldPeripheral nervous system developmentNerve injuryPain modelNerve crushPain managementAntibody treatmentGait recovery
2006
Electrical impedance myography: Transitioning from human to animal studies
Nie R, Sunmonu N, Chin A, Lee K, Rutkove S. Electrical impedance myography: Transitioning from human to animal studies. Clinical Neurophysiology 2006, 117: 1844-1849. PMID: 16807097, DOI: 10.1016/j.clinph.2006.03.024.Peer-Reviewed Original ResearchConceptsElectrical impedance myographyHamstring musclesRat models of neuromuscular diseasesSciatic nerve crushPrimary outcome variableHealthy adult ratsHuman subjectsAdult ratsNerve crushModels of neuromuscular diseasesRat modelNeurogenic injuryOutcome variablesAnimal studiesRatsImpedance myographyEIM dataPost-injuryRat muscleNeuromuscular diseaseMuscleHamstringRecordsSurface recordingsSubjects
2003
Nogo-C is sufficient to delay nerve regeneration
Kim J, Bonilla IE, Qiu D, Strittmatter SM. Nogo-C is sufficient to delay nerve regeneration. Molecular And Cellular Neuroscience 2003, 23: 451-459. PMID: 12837628, DOI: 10.1016/s1044-7431(03)00076-9.Peer-Reviewed Original ResearchConceptsAxonal regenerationTransgenic miceSciatic nerve injurySciatic nerve crushAxon growth inhibitorsWild-type miceCentral nervous systemC transgenic miceDecreased recovery ratePeripheral Schwann cellsNerve injuryNerve crushMotor functionPeripheral clearanceSchwann cellsCNS expressionNerve regenerationNervous systemAdult mammalsMiceNogoCellsGrowth inhibitorExpressionInjury
2002
Regenerating nerves follow the road more traveled
Fournier AE, Strittmatter SM. Regenerating nerves follow the road more traveled. Nature Neuroscience 2002, 5: 821-822. PMID: 12196804, DOI: 10.1038/nn0902-821.Peer-Reviewed Original Research
2000
Nerve growth factor maintains potassium conductance after nerve injury in adult cutaneous afferent dorsal root ganglion neurons
Everill B, Kocsis J. Nerve growth factor maintains potassium conductance after nerve injury in adult cutaneous afferent dorsal root ganglion neurons. Neuroscience 2000, 100: 417-422. PMID: 11008179, PMCID: PMC2605351, DOI: 10.1016/s0306-4522(00)00263-3.Peer-Reviewed Original ResearchConceptsTransient A-currentNerve growth factorDorsal root ganglion neuronsK currentsNerve ligationGrowth factorA-currentGanglion neuronsSciatic nerveWhole-cell patch-clamp techniquePotassium conductanceDistal nerve segmentsAfferent cell bodiesMini-osmotic pumpsVoltage-dependent potassium conductancePatch-clamp techniqueTransient potassium currentAppropriate ion replacementNerve growth factor treatmentGrowth factor treatmentNerve injuryNerve crushNerve segmentsCutaneous afferentsControl neurons
1998
Cloning of Zebrafish Neurofilament cDNAs for Plasticin and Gefiltin: Increased mRNA Expression in Ganglion Cells After Optic Nerve Injury
Asch W, Leake D, Canger A, Passini M, Argenton F, Schechter N. Cloning of Zebrafish Neurofilament cDNAs for Plasticin and Gefiltin: Increased mRNA Expression in Ganglion Cells After Optic Nerve Injury. Journal Of Neurochemistry 1998, 71: 20-32. PMID: 9648847, DOI: 10.1046/j.1471-4159.1998.71010020.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCloning, MolecularDNA, ComplementaryEye ProteinsFish ProteinsGene Expression RegulationGoldfishIntermediate Filament ProteinsMolecular Sequence DataNerve CrushNerve RegenerationNerve Tissue ProteinsNeurofilament ProteinsOptic NerveOptic Nerve InjuriesRetinal Ganglion CellsRNA, MessengerSequence Homology, Amino AcidVisual PathwaysZebrafishConceptsNeuronal intermediate filament proteinOptic nerve crushOptic nerve injuryGoldfish visual pathwayIntermediate filament proteinsOptic axon regenerationIntermediate filament compositionNerve crushNerve injuryUnoperated eyesGanglion cellsGefiltinFilament proteinsAxon regenerationRetinal growthBaseline levelsVisual pathwayAxonal growthSoluble factorsMRNA expressionMRNA levels
1997
Timing of c-jun protein induction in lumbar dorsal root ganglia after sciatic nerve transection varies with lesion distance
Kenney A, Kocsis J. Timing of c-jun protein induction in lumbar dorsal root ganglia after sciatic nerve transection varies with lesion distance. Brain Research 1997, 751: 90-95. PMID: 9098571, DOI: 10.1016/s0006-8993(96)01402-3.Peer-Reviewed Original ResearchConceptsSciatic nerve transectionNerve transectionDorsal root gangliaNerve injuryRoot gangliaLumbar dorsal root ganglion neuronsL5 dorsal root gangliaLumbar dorsal root gangliaDorsal root ganglion neuronsSciatic nerve injurySciatic nerve crushC-Jun proteinProtein inductionC-Jun responseNerve crushGanglion neuronsImmunohistochemical studyLesion distanceTransectionInduces upregulationEarly increaseC-jun mRNAEarly inductionChemiluminescent immunoblottingGanglia
1991
Tea‐sensitive potassium channels and inward rectification in regenerated rat sciatic nerve
Gardon T, Kocsis J, Waxman S. Tea‐sensitive potassium channels and inward rectification in regenerated rat sciatic nerve. Muscle & Nerve 1991, 14: 640-646. PMID: 1922170, DOI: 10.1002/mus.880140707.Peer-Reviewed Original ResearchConceptsCompound action potentialRat sciatic nerveNerve crushRegenerated axonsSciatic nerveRegenerated nervesInward rectificationIntra-axonal recording techniquesAdult rat sciatic nerveTEA-sensitive potassium channelsPotassium channelsRegenerated rat sciatic nerveSucrose gap recordingsSciatic nerve crushPeripheral nerve axonsWhole nerve recordingsIntra-axonal recordingsVoltage-sensitive sodium channelsCrush injuryNormal nervesSensitive relaxationRepetitive stimulationAfterhyperpolarizationGap recordingsNerve recordings
1990
Trophic influence of the distal nerve segment on GABAA receptor expression in axotomized adult sensory neurons
Bhisitkul R, Kocsis J, Gordon T, Waxman S. Trophic influence of the distal nerve segment on GABAA receptor expression in axotomized adult sensory neurons. Experimental Neurology 1990, 109: 273-278. PMID: 2170161, DOI: 10.1016/s0014-4886(05)80017-2.Peer-Reviewed Original ResearchConceptsDistal nerve segmentsGamma-aminobutyric acidNerve segmentsSciatic nerveReceptor expressionSensory neuronsTrophic supportGABAA receptor agonist muscimolDorsal root ganglion neuronsAxotomized sensory neuronsReactive Schwann cellsGABAA receptor expressionAdult sensory neuronsReceptor agonist muscimolExpression of receptorsPeripheral target tissuesGABA receptor expressionDorsal root fibersGABA receptor sensitivityEnd organ innervationPostoperative dayNerve crushNerve transectionCrush siteDistal stump
1987
Axonal GABA receptors are selectively present on normal and regenerated sensory fibers in rat peripheral nerve
Bhisitkul R, Villa J, Kocsis J. Axonal GABA receptors are selectively present on normal and regenerated sensory fibers in rat peripheral nerve. Experimental Brain Research 1987, 66: 659-663. PMID: 3038587, DOI: 10.1007/bf00270698.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidVentral root fibersGABA receptorsRoot fibersSensory fibersPeripheral nervesSensory axonsRegenerated sensory axonsSucrose gap chamberPeripheral nerve fibersRat peripheral nerveDorsal root fibersMammalian peripheral nervesAgonist baclofenNerve crushDorsal rootsAgonist muscimolSciatic nerveNerve fibersRat peripheral nerve fibersNerveReceptorsMuscimolSelective presenceAxons
1983
Long-term regenerated nerve fibres retain sensitivity to potassium channel blocking agents
Kocsis J, Waxman S. Long-term regenerated nerve fibres retain sensitivity to potassium channel blocking agents. Nature 1983, 304: 640-642. PMID: 6308475, DOI: 10.1038/304640a0.Peer-Reviewed Original ResearchConceptsNerve fibersPotassium channelsMyelinated peripheral nerve fibresAxon segmentsPeripheral nerve fibersAxon sproutsEndoneurial tubesNerve crushFunctional recoveryFunctional organizationMyelinated fibersAxon cylindersSchwann cellsBurst activityMyelinated axonsMammalian axonsAxonsPeripheral connectionsMembrane depolarizationBasement membraneK channelsRegenerated fibersAxon maturation
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