2025
Investigation of the protein corona and biodistribution profile of polymeric nanoparticles for intra-amniotic delivery
Lynn A, Shin K, Eaton D, Rose M, Zhang X, Ene M, Grundler J, Deschenes E, Rivero R, Bracaglia L, Glazer P, Stitelman D, Saltzman W. Investigation of the protein corona and biodistribution profile of polymeric nanoparticles for intra-amniotic delivery. Biomaterials 2025, 320: 123238. PMID: 40064138, PMCID: PMC11972154, DOI: 10.1016/j.biomaterials.2025.123238.Peer-Reviewed Original ResearchAmniotic fluidProtein corona formationProtein coronaIntra-amniotic deliveryNP delivery systemPEGylated nanoparticlesSurface polyethylene glycolPoly(lactic-co-glycolic acidProtein corona compositionLung cells in vitroCorona formationPLA-PEG nanoparticlesNon-PEGylated nanoparticlesCells in vitroLevels of albuminFetal bowelFetal lungFetal organsNP biodistributionPoly(lactic-acidDense brush conformationCorona compositionPolyvinyl alcoholBiodistribution profileCellular association
2023
Amniotic fluid cortisol predicts neonatal and infant development in non-stressed rhesus monkeys: Implications for prenatal stress
Otridge J, Meyer J, Dettmer A. Amniotic fluid cortisol predicts neonatal and infant development in non-stressed rhesus monkeys: Implications for prenatal stress. Neurotoxicology And Teratology 2023, 100: 107308. PMID: 37890675, PMCID: PMC10872548, DOI: 10.1016/j.ntt.2023.107308.Peer-Reviewed Original ResearchConceptsHair cortisol concentrationsCORT exposureInfant developmentPrenatal stressMaternal hair cortisol concentrationsAmniotic fluid cortisolInfant hair cortisol concentrationsFetal cortisol exposurePostnatal day 30Healthy rhesus macaquesInfant growth rateHealthy primatesGestational ageClinical reasonsCortisol exposureDays gestationNeonatal assessmentSensorimotor scoresAmniotic fluidNeurological developmentCortisol concentrationsDay 30Rhesus monkeysRhesus macaquesCORT levels
2020
Obstetric Emergencies
Kuschner Z, Bassily‐Marcus A. Obstetric Emergencies. 2020, 377-386. DOI: 10.1002/9781119293255.ch41.Peer-Reviewed Original ResearchMaternal cardiac arrestPre-eclampsiaObstetric emergenciesCardiac arrestFetal amniotic fluidPost-partum hemorrhagePulmonary embolic diseasePeri-partum cardiomyopathyNational society guidelinesAmniotic fluidPulmonary arteryEmbolic diseaseEmbolic occlusionSociety guidelinesReversible causesVenous thrombiEarly treatmentMechanical obstructionMyocardial depressionEndovascular dysfunctionEclampsiaArrestTreatmentCardiomyopathyHemorrhage
2019
Fetal Myocardial Function as Assessed by N-Terminal Fragment Brain Natriuretic Protein in Premature Fetuses Exposed to Intra-amniotic Inflammation
Irani RA, Buhimschi CS, Cross SN, Zhao G, Copel JA, Bhandari V, Buhimschi IA, Bahtiyar MO. Fetal Myocardial Function as Assessed by N-Terminal Fragment Brain Natriuretic Protein in Premature Fetuses Exposed to Intra-amniotic Inflammation. American Journal Of Perinatology 2019, 37: 745-753. PMID: 31121635, DOI: 10.1055/s-0039-1688909.Peer-Reviewed Original ResearchMeSH KeywordsAdultAmniocentesisAmniotic FluidBiomarkersChorioamnionitisEchocardiography, DopplerFemaleFetal BloodFetal HeartHumansInfant, NewbornInfant, PrematureInflammationInterleukin-6MaleMass SpectrometryNatriuretic Peptide, BrainPeptide FragmentsPlacentaPregnancyPremature BirthVentricular Function, LeftConceptsIntra-amniotic infection/inflammationBrain natriuretic proteinAmniotic fluidNT-proBNPTei indexNatriuretic proteinVentricular functionFetal exposureCord bloodCord IL-6Intra-amniotic inflammationFetal myocardial functionInfection/inflammationBlood NT-proBNPFetal heart functionNT-proBNP immunoassaysEnzyme-linked immunosorbentGestational agePremature fetusesFetal echocardiogramIL-6Circulatory levelsMyocardial functionHeart functionFetuses
2018
Tenascin-X in amniotic fluid and reproductive tissues of pregnancies complicated by infection and preterm prelabor rupture of membranes†
Rood KM, Buhimschi CS, Zhao G, Oliver EA, Summerfield T, Bahtiyar MO, Buhimschi IA. Tenascin-X in amniotic fluid and reproductive tissues of pregnancies complicated by infection and preterm prelabor rupture of membranes†. Biology Of Reproduction 2018, 100: 773-782. PMID: 30277495, PMCID: PMC6437262, DOI: 10.1093/biolre/ioy216.Peer-Reviewed Original ResearchConceptsIntra-amniotic infection/inflammationPreterm prelabor ruptureFetal membranesAmniotic fluidTNX expressionPrelabor ruptureInfection/inflammationLung maturity testingMembrane statusSpontaneous PPROMPregnancy complicationsNormal outcomeTherapeutic targetGenetic karyotypingReproductive tissuesPPROMMRNA expressionMaturity testingPregnancyWestern blottingMRNA levelsQRT-PCRExtracellular matrix proteinsCervixInflammation
2015
Carcinoembryonic antigen as a biomarker for meconium‐stained amniotic fluid
Mor A, Tal R, Irani M, McCalla S, Haberman S, Garg D, Wajntraub B. Carcinoembryonic antigen as a biomarker for meconium‐stained amniotic fluid. International Journal Of Gynecology & Obstetrics 2015, 132: 329-331. PMID: 26674317, DOI: 10.1016/j.ijgo.2015.07.032.Peer-Reviewed Original ResearchConceptsMeconium-stained amniotic fluidClear amniotic fluidAmniotic fluidCEA concentrationHigh CEA concentrationsMean CEA concentrationsTerm singleton pregnanciesProspective cohort studyRupture of membranesClear medical indicationCarcinoembryonic antigen concentrationCharacteristic curve analysisAmniotic fluid statusCEA cutoffSingleton pregnanciesCohort studyArtificial ruptureBedside testFluid statusNormal rangeMedical indicationsCarcinoembryonic antigenAntigen concentrationCurve analysisOnly womenAlpha-Fetoprotein as a Tool to Distinguish Amniotic Fluid From Urine, Vaginal Discharge, and Semen
Mor A, Tal R, Haberman S, McCalla S, Irani M, Perlman J, Seifer DB, Minkoff H. Alpha-Fetoprotein as a Tool to Distinguish Amniotic Fluid From Urine, Vaginal Discharge, and Semen. Obstetrics And Gynecology 2015, 125: 448-452. PMID: 25569004, DOI: 10.1097/aog.0000000000000635.Peer-Reviewed Original ResearchConceptsNormal vaginal dischargeAlpha-fetoprotein concentrationDiagnosis of ruptureVaginal dischargeAmniotic fluidAlpha-fetoproteinPregnant womenReceiver operator characteristic curve analysisSanitary padsOperator characteristic curve analysisProspective cohort studyAlpha-fetoprotein levelsAmniotic fluid levelsCharacteristic curve analysisAmniotic fluid specimensCohort studyInfertility evaluationMaternal urineAFP levelsFluid specimensFluid levelsSemen specimensCurve analysisUrineAFP quantification
2012
Prenatal versus postnatal sex steroid hormone effects on autistic traits in children at 18 to 24 months of age
Auyeung B, Ahluwalia J, Thomson L, Taylor K, Hackett G, O’Donnell K, Baron-Cohen S. Prenatal versus postnatal sex steroid hormone effects on autistic traits in children at 18 to 24 months of age. Molecular Autism 2012, 3: 17. PMID: 23231861, PMCID: PMC3554559, DOI: 10.1186/2040-2392-3-17.Peer-Reviewed Original ResearchMonths of ageAndrogen exposureSex steroid hormone effectsFT levelsSex steroid hormone levelsSex steroid hormonesSteroid hormone levelsSecond trimester amniocentesisSteroid hormone effectsMonths old toddlersPostnatal androgen exposurePrenatal androgen exposureConclusionsThese preliminary findingsEstradiol levelsPostnatal exposurePregnant womenPrenatal exposureHormone levelsPostnatal testosterone levelsTestosterone levelsAndrogen hormonesAmniotic fluidLarger sample populationHormone effectsSteroid hormones51: Evidence for the presence of super-interleukin-6 (superIL-6) trans-signaling complex in amniotic fluid (AF) and its participation in the intraamniotic inflammatory response to infection
Abdel-Razeq S, Buhimschi I, Trotta K, Zhao G, Bahtiyar M, Pettker C, Buhimschi C. 51: Evidence for the presence of super-interleukin-6 (superIL-6) trans-signaling complex in amniotic fluid (AF) and its participation in the intraamniotic inflammatory response to infection. American Journal Of Obstetrics And Gynecology 2012, 206: s32-s33. DOI: 10.1016/j.ajog.2011.10.077.Peer-Reviewed Original Research
2010
Interpretation of Amniotic Fluid White Blood Cell Count in “Bloody Tap” Amniocenteses in Women With Symptoms of Preterm Labor
Abdel-Razeq SS, Buhimschi IA, Bahtiyar MO, Rosenberg VA, Dulay AT, Han CS, Werner EF, Thung S, Buhimschi CS. Interpretation of Amniotic Fluid White Blood Cell Count in “Bloody Tap” Amniocenteses in Women With Symptoms of Preterm Labor. Obstetrics And Gynecology 2010, 116: 344-354. PMID: 20664395, PMCID: PMC4142228, DOI: 10.1097/aog.0b013e3181e8fec6.Peer-Reviewed Original ResearchConceptsWhite blood cellsAmniotic fluid white blood cellBlood cell countWBC countAmniotic fluidBloody tapAmniotic fluid white blood cell countCell countAmniotic fluid WBC countRBC/White blood cell countPositive amniotic fluid cultureMaternal WBC countRed blood cell countCells/Number of neutrophilsAbsence of inflammationSetting of inflammationAmniotic fluid culturePositive likelihood ratioAmniotic fluid samplesIntraamniotic inflammationPreterm laborConsecutive womenFluid culture
2009
527: Interleukin-6 trans-signaling molecules soluble GP130 and soluble IL-6 receptor in the amniotic fluid (AF) of women with intra-amniotic inflammation (IAI) and preterm premature rupture of membranes (PPROM)
Lee S, Buhimschi I, Zhao G, Dulay A, Bahtiyar M, Han C, Buhimschi C. 527: Interleukin-6 trans-signaling molecules soluble GP130 and soluble IL-6 receptor in the amniotic fluid (AF) of women with intra-amniotic inflammation (IAI) and preterm premature rupture of membranes (PPROM). American Journal Of Obstetrics And Gynecology 2009, 201: s196. DOI: 10.1016/j.ajog.2009.10.693.Peer-Reviewed Original ResearchUsing proteomics in perinatal and neonatal sepsis: hopes and challenges for the future
Buhimschi CS, Bhandari V, Han YW, Dulay AT, Baumbusch MA, Madri JA, Buhimschi IA. Using proteomics in perinatal and neonatal sepsis: hopes and challenges for the future. Current Opinion In Infectious Diseases 2009, 22: 235-243. PMID: 19395960, PMCID: PMC2763282, DOI: 10.1097/qco.0b013e32832a5963.Peer-Reviewed Original ResearchConceptsNeonatal sepsisEarly-onset neonatal sepsisInsulin-like growth factor-binding protein-1Growth factor-binding protein-1Proteomic biomarkersAdvanced glycation end productsAmniotic fluid biomarkersAmniotic fluid inflammationFetal immune responsePoor neurodevelopmental outcomesIntra-amniotic infectionRisk of sepsisGlycation end productsHistological chorioamnionitisPreterm birthInflammatory statusNeurodevelopmental outcomesNeuroprotection strategiesInflammatory stateNeurological injuryFluid biomarkersInflammatory processImmune responseAmniotic fluidDefensin 2
2008
Multidimensional Proteomics Analysis of Amniotic Fluid to Provide Insight into the Mechanisms of Idiopathic Preterm Birth
Buhimschi IA, Zhao G, Rosenberg VA, Abdel-Razeq S, Thung S, Buhimschi CS. Multidimensional Proteomics Analysis of Amniotic Fluid to Provide Insight into the Mechanisms of Idiopathic Preterm Birth. PLOS ONE 2008, 3: e2049. PMID: 18431506, PMCID: PMC2315798, DOI: 10.1371/journal.pone.0002049.Peer-Reviewed Original ResearchMeSH KeywordsAdultAmniotic FluidBiomarkersDown-RegulationElectrophoresis, Gel, Two-DimensionalFemaleHemorrhageHumansInflammationPlacentaPregnancyPregnancy ProteinsPremature BirthProteomeProteomicsRNA, MessengerSoftwareSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTreatment OutcomeUmbilical CordUp-RegulationConceptsPreterm birthAmniotic fluidIdiopathic preterm birthIntra-amniotic inflammationMost preterm birthsSubgroup of patientsAbsence of inflammationDistinct pathogenetic mechanismsFuture drug developmentProteomic fingerprintExpectant managementNovel pathogenetic pathwaysPreterm laborPremature ruptureClinical resultsPathogenetic mechanismsPathogenetic pathwaysConsecutive sampleTherapeutical interventionsInflammationSELDI peaksBirthBleedingProtein metabolismDrug developmentUsing Proteomic Analysis of the Human Amniotic Fluid to Identify Histologic Chorioamnionitis
Buhimschi IA, Zambrano E, Pettker CM, Bahtiyar MO, Paidas M, Rosenberg VA, Thung S, Salafia CM, Buhimschi CS. Using Proteomic Analysis of the Human Amniotic Fluid to Identify Histologic Chorioamnionitis. Obstetrics And Gynecology 2008, 111: 403-412. PMID: 18238979, DOI: 10.1097/aog.0b013e31816102aa.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAmniocentesisAmniotic FluidBiomarkersBlack or African AmericanChorioamnionitisFemaleHumansInflammationPregnancyPregnancy Complications, InfectiousPregnancy OutcomePrevalenceProteomeProteomicsReproducibility of ResultsSensitivity and SpecificitySeverity of Illness IndexSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationWhite PeopleConceptsHistologic chorioamnionitisAmniotic fluidIntra-amniotic inflammationGrade of inflammationAbsence of biomarkersHuman amniotic fluidSurface-enhanced laser desorption ionization timeAfrican American womenPreterm laborSingleton pregnanciesDelivery intervalPlacental pathologyGestational agePremature ruptureSevere inflammationEarly recognitionChorioamnionitisChorionic plateInflammationTherapeutic interventionsProteomic biomarkersCalgranulin CBiomarkersProteomic analysisScores
2007
Proteomic Profiling of the Amniotic Fluid to Detect Inflammation, Infection, and Neonatal Sepsis
Buhimschi CS, Bhandari V, Hamar BD, Bahtiyar MO, Zhao G, Sfakianaki AK, Pettker CM, Magloire L, Funai E, Norwitz ER, Paidas M, Copel JA, Weiner CP, Lockwood CJ, Buhimschi IA. Proteomic Profiling of the Amniotic Fluid to Detect Inflammation, Infection, and Neonatal Sepsis. PLOS Medicine 2007, 4: e18. PMID: 17227133, PMCID: PMC1769412, DOI: 10.1371/journal.pmed.0040018.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAmniocentesisAmniotic FluidBiomarkersFemaleHumansInflammationMiddle AgedPregnancyPregnancy Complications, InfectiousPregnancy OutcomeProteomeProteomicsReproducibility of ResultsSensitivity and SpecificitySepsisSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationConceptsEarly-onset neonatal sepsisAmniotic fluid inflammationIntra-amniotic infectionMR scoreNeonatal sepsisAmniotic fluidHistological chorioamnionitisDelivery intervalNewborn Special Care UnitGram stainEarly-onset sepsisIntra-amniotic inflammationDegree of inflammationSpecial care unitsIntrauterine inflammationPlacental inflammationShorter amniocentesisPreterm deliveryPreterm laborSingleton pregnanciesConsecutive womenPreterm birthCare unitGestational agePremature rupture
2006
Angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2) in amniotic fluid. Identification and regulation during pregnancy and intra-amniotic infection
Buhimschi C, Bhandari V, Funai E, Zhao G, Rosenberg V, Cackovic M, Magriples U, Paidas M, Buhimschi I. Angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2) in amniotic fluid. Identification and regulation during pregnancy and intra-amniotic infection. American Journal Of Obstetrics And Gynecology 2006, 195: s158. DOI: 10.1016/j.ajog.2006.10.572.Peer-Reviewed Original Research
2001
57 Detection of viral deoxyribonucleic acid in amniotic fluid: Association with fetal malformation and pregnancy abnormalities
Reddy U, Baschat A, Towbin J, Harman C, Weiner C. 57 Detection of viral deoxyribonucleic acid in amniotic fluid: Association with fetal malformation and pregnancy abnormalities. American Journal Of Obstetrics And Gynecology 2001, 185: s95. DOI: 10.1016/s0002-9378(01)80092-2.Peer-Reviewed Original ResearchIn vitro sealing of punctured fetal membranes: Potential treatment for midtrimester premature rupture of membranes
Reddy U, Shah S, Nemiroff R, Ballas S, Hyslop T, Chen J, Wapner R, Sciscione A. In vitro sealing of punctured fetal membranes: Potential treatment for midtrimester premature rupture of membranes. American Journal Of Obstetrics And Gynecology 2001, 185: 1090-1093. PMID: 11717639, DOI: 10.1067/mob.2001.117685.Peer-Reviewed Original ResearchConceptsMidtrimester premature ruptureAmniotic fluidPremature ruptureFetal membranesAmniotic membraneSignificant perinatal morbidityPrimary outcome variableSecond trimester pregnancySecond trimester amniotic fluidRank sum testPerinatal morbidityMedian timeTrimester pregnancyPuncture siteEffective treatmentPotential treatmentNeedle punctureOutcome variablesSum test
2000
Fetal arterial and venous Doppler parameters in the interpretation of oligohydramnios in postterm pregnancies
Selam B, Koksal R, Ozcan T. Fetal arterial and venous Doppler parameters in the interpretation of oligohydramnios in postterm pregnancies. Ultrasound In Obstetrics And Gynecology 2000, 15: 403-406. PMID: 10976482, DOI: 10.1046/j.1469-0705.2000.00121.x.Peer-Reviewed Original ResearchConceptsAmniotic fluid indexInferior vena cavaMiddle cerebral arteryVenous Doppler parametersPulsatility indexPostterm pregnancyDuctus venosusMCA PI/UA PI ratioSemiquantitative assessment of amniotic fluid volumeAssessment of amniotic fluid volumeRedistribution of fetal blood flowFetal inferior vena cavaFetal middle cerebral arteryDoppler parametersAmniotic fluid volumeCases of oligohydramniosRenal artery PIFetal blood flowPost-term pregnancyPresence of oligohydramniosBrain sparing effectPulsed Doppler imagingCross-sectional studyUmbilical arteryAmniotic fluid
1999
Prostate-specific antigen (PSA) in women.
Yu H, Berkel H. Prostate-specific antigen (PSA) in women. Journal Of The Louisiana State Medical Society 1999, 151: 209-13. PMID: 10234897.Peer-Reviewed Original ResearchConceptsProstate-specific antigenBreast cancerPSA levelsProduction of PSABetter disease-free survivalSerum prostate-specific antigenBreast tissueDisease-free survivalBreast cancer riskValuable tumor markerExcess androgenOverall survivalGestational agePregnant womenBreast fluidProgesterone receptorMenstrual cycleNipple aspiratesProstate cancerClinical studiesEstrogen receptorCancer riskTumor markersCyst fluidAmniotic fluid
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