Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5'-nucleotidase in myeloid leukocytes
Clifford E, Martin K, Dalal P, Thomas R, Dubyak G. Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5'-nucleotidase in myeloid leukocytes. American Journal Of Physiology 1997, 273: c973-c987. PMID: 9316419, DOI: 10.1152/ajpcell.1997.273.3.c973.Peer-Reviewed Original ResearchMeSH Keywords5'-NucleotidaseAdenosine DiphosphateAdenosine MonophosphateAdenosine TriphosphatasesAdenosine TriphosphateAntigens, CDApyraseBucladesineCell DifferentiationCytosolDNA PrimersGene Expression Regulation, NeoplasticHL-60 CellsHumansLeukemiaLeukocytesModels, BiologicalPolymerase Chain ReactionReceptors, Purinergic P2RNA, MessengerTetradecanoylphorbol AcetateTime FactorsTranscription, GeneticTumor Cells, CulturedUridine TriphosphateConceptsKG-1 cellsMyeloid progenitorsStage-specific expressionHL-60 promyelocytesExtracellular ATPMyeloid cell differentiationHL-60P2-purinergic receptor subtypesSignal transductionMyeloid leukocytesHL-60 cellsTranscription-polymerase chain reaction analysisATP receptor subtypesExogenous ATPaseCell differentiationRecent identificationDevelopmental stagesApyrase activityTHP-1 monocytesChain reaction analysisPotato apyraseP2Y receptor subtypesPurinergic receptor subtypesDifferentiationAutocrine mechanismDown-Regulation of P2U-Purinergic Nucleotide Receptor Messenger RNA Expression During In Vitro Differentiation of Human Myeloid Leukocytes by Phorbol Esters or Inflammatory Activators
Martin K, Kertesy S, Dubyak G. Down-Regulation of P2U-Purinergic Nucleotide Receptor Messenger RNA Expression During In Vitro Differentiation of Human Myeloid Leukocytes by Phorbol Esters or Inflammatory Activators. Molecular Pharmacology 1997, 51: 97-108. PMID: 9016351, DOI: 10.1124/mol.51.1.97.Peer-Reviewed Original ResearchConceptsTHP-1 monocytesHL-60 cellsMRNA levelsMyeloid leukocytesReceptor messenger RNA expressionMonocyte/macrophage phenotypeMessenger RNA expressionInositol phospholipid hydrolysisNeutrophil phenotypeInflammatory activationHL-60 granulocytesUndifferentiated HL-60 cellsInflammatory activatorsInflammatory macrophagesAcute exposureMacrophage phenotypeNucleotide receptorsHuman epithelial cellsTissue-specific mechanismsEpithelial cellsRNA expressionLeukocytesDown regulationDibutyryl cAMPHuman keratinocytes