1999
Regulation of Neurabin I Interaction with Protein Phosphatase 1 by Phosphorylation †
McAvoy T, Allen P, Obaishi H, Nakanishi H, Takai Y, Greengard P, Nairn A, Hemmings H. Regulation of Neurabin I Interaction with Protein Phosphatase 1 by Phosphorylation †. Biochemistry 1999, 38: 12943-12949. PMID: 10504266, DOI: 10.1021/bi991227d.Peer-Reviewed Original ResearchConceptsProtein phosphatase 1Neurabin IPP1 activityPhosphatase 1Two-hybrid interaction analysisActin-binding proteinsCo-immunoprecipitation experimentsMimic phosphorylationSerine 461Phosphorylated residuesGlutathione S-transferaseOverlay assaysFusion proteinSignaling mechanismGamma isoformsCAMP pathwayPhosphorylationS-transferaseProteinTryptic digestPKARegulationHPLC-MS analysisInteraction analysisS461
1988
Ca2+/calmodulin-dependent protein kinase II: identification of threonine-286 as the autophosphorylation site in the alpha subunit associated with the generation of Ca2+-independent activity.
Thiel G, Czernik AJ, Gorelick F, Nairn AC, Greengard P. Ca2+/calmodulin-dependent protein kinase II: identification of threonine-286 as the autophosphorylation site in the alpha subunit associated with the generation of Ca2+-independent activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 6337-6341. PMID: 2842767, PMCID: PMC281965, DOI: 10.1073/pnas.85.17.6337.Peer-Reviewed Original ResearchConceptsBeta/beta' subunitsAlpha subunitThr-286Beta subunitDependent protein kinase IIProtein kinase IIAutophosphorylation sitesThreonine residuesMajor phosphopeptideNaDodSO4/PAGEPhosphorylated residuesCyanogen bromide peptidesConsensus sequenceKinase IIIndependent activityThermolytic phosphopeptidesPrimary structureGas-phase Edman degradationGeneration of Ca2Edman degradationAutophosphorylationSubunitsThreonine-286Amino acidsAsp-Xaa