2025
Macromolecular crowding-based biofabrication utilizing unmodified extracellular matrix bioinks
Jordan S, Li X, Rossello-Martinez A, Liang Z, Gong X, Xiao H, Mak M. Macromolecular crowding-based biofabrication utilizing unmodified extracellular matrix bioinks. Acta Biomaterialia 2025, 198: 37-48. PMID: 40268621, DOI: 10.1016/j.actbio.2025.02.052.Peer-Reviewed Original ResearchConceptsDecellularized extracellular matrixExtrusion bioprintingComplex 3D structuresLayer-by-layer buildingCell scaffold materialsNatural extracellular matrixBiofabrication applicationsSolubilized extracellular matrixRobust hydrogelsLow printabilityTissue engineeringBioinkFabrication methodPromote biocompatibilityGelation timeBioactive scaffoldsPrintabilityBiofabricationNative tissueOrgan-specific extracellular matrixCrosslinkingExtracellular matrixBiocompatibilityExtrusionRegenerative medicineFibronectin matrix assembly at a glance.
Sun Y, Hamlin A, Schwarzbauer J. Fibronectin matrix assembly at a glance. Journal Of Cell Science 2025, 138 PMID: 40130407, PMCID: PMC12050093, DOI: 10.1242/jcs.263834.Peer-Reviewed Original ResearchConceptsAbundant ECM proteinFibronectin matrix assemblyOrganization of FNExtracellular matrixMechanisms of extracellular matrixSecrete ECM componentsFN assemblyDomain organizationFN fibrilsFN matrixGlance articleECM proteinsMatrix assemblyECM componentsIntracellular pathwaysFibrillar matrixReceptor-mediated processFibronectinCell scienceProteinAssemblyCellsCell-mediated processPathwayMechanismGeneration of Orthogonal Gradients of the Matrix Stiffness and Chemotactic Cues in a Suspended Array of Hydrogel to Study hMSCs Migration
Xu Z, Ponek A, Thomas J, Qyang Y. Generation of Orthogonal Gradients of the Matrix Stiffness and Chemotactic Cues in a Suspended Array of Hydrogel to Study hMSCs Migration. ACS Sensors 2025, 10: 1722-1728. PMID: 40021359, DOI: 10.1021/acssensors.4c02793.Peer-Reviewed Original ResearchMeSH KeywordsCell MovementChemokine CXCL12ChemotaxisDimethylpolysiloxanesExtracellular MatrixHumansHydrogelsLab-On-A-Chip DevicesMesenchymal Stem CellsConceptsMatrix stiffnessChemotactic cuesHuman mesenchymal stem cellsHydrogel cylindersCell migrationDevice working principleMigration of human mesenchymal stem cellsFactor 1 alphaCellular contextOrthogonal gradientsReplica moldingGlass substratesCell migration studiesDevice fabricationWorking principleMicrofluidic devicesIn vitro assaysHydrogelsStiffnessCell typesSuspended arrayMesenchymal stem cellsConcentration gradientPoly(dimethylsiloxaneCylinderMorphogenesis of confined biofilms: how mechanical interactions determine cellular patterning and global geometry
Nam K, Yan J. Morphogenesis of confined biofilms: how mechanical interactions determine cellular patterning and global geometry. Soft Matter 2025, 21: 1436-1450. PMID: 39901805, PMCID: PMC11791476, DOI: 10.1039/d4sm01180e.Peer-Reviewed Original ResearchConceptsVibrio cholerae</i> biofilmsBiofilm developmentTransmission of mechanical stressExtracellular matrixSingle-cell levelBacterial communitiesDevelopmental biologyBacterial coloniesBiofilmSingle-cellReview recent studiesMechanical interplayFluorescence microscopyMorphogenesisActive nematicsGlobal geometryCellular patternOrientational orderContinuum levelVibrioConfining mediumRecent studiesMechanical interactionShort-term disruption of TGFβ signaling in adult mice renders the aorta vulnerable to hypertension-induced dissection
Jiang B, Ren P, He C, Wang M, Murtada S, Ruiz-Rodríguez M, Chen Y, Ramachandra A, Li G, Qin L, Assi R, Schwartz M, Humphrey J, Tellides G. Short-term disruption of TGFβ signaling in adult mice renders the aorta vulnerable to hypertension-induced dissection. JCI Insight 2025, 10 PMID: 39932797, PMCID: PMC11949005, DOI: 10.1172/jci.insight.182629.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsBlood pressureAortic dissectionAdult miceInherited connective tissue disorderConnective tissue disordersTGF-b signalingAccumulation of bloodHigh blood pressureAortic phenotypeTissue disordersMolecule expressionTGFB signalingMuscle cellsRisk factorsSynthesis of extracellular matrixSustained increaseTransient increaseBlood extravasationDissectionMedial injuryExtracellular matrix productionVascular degenerationExperimental modelMiceSurface remodeling and inversion of cell-matrix interactions underlie community recognition and dispersal in Vibrio cholerae biofilms
Moreau A, Nguyen D, Hinbest A, Zamora A, Weerasekera R, Matej K, Zhou X, Sanchez S, Rodriguez Brenes I, Tai J, Nadell C, Ng W, Gordon V, Komarova N, Olson R, Li Y, Yan J. Surface remodeling and inversion of cell-matrix interactions underlie community recognition and dispersal in Vibrio cholerae biofilms. Nature Communications 2025, 16: 327. PMID: 39747177, PMCID: PMC11695861, DOI: 10.1038/s41467-024-55602-2.Peer-Reviewed Original ResearchMeSH KeywordsBacterial AdhesionBacterial ProteinsBiofilmsExtracellular MatrixPolysaccharide-LyasesPolysaccharides, BacterialVibrio choleraeConceptsCell-matrix interactionsCausative agent of choleraSurface-associated bacterial communitiesAgent of choleraV. cholerae cellsVibrio cholerae biofilmsSurface remodelingInvestigate cell-matrix interactionsBiofilm cellsBacterial communitiesVibrio choleraeBiofilm developmentBiofilmCausative agentBiofilm growthBiochemical analysisExtracellular matrixVibrioBiofilm ageCell dispersionMatrix componentsCholeraCellsRbmBShed light
2024
Ezrin drives adaptation of monocytes to the inflamed lung microenvironment
Gudneppanavar R, Di Pietro C, H Öz H, Zhang P, Cheng E, Huang P, Tebaldi T, Biancon G, Halene S, Hoppe A, Kim C, Gonzalez A, Krause D, Egan M, Gupta N, Murray T, Bruscia E. Ezrin drives adaptation of monocytes to the inflamed lung microenvironment. Cell Death & Disease 2024, 15: 864. PMID: 39613751, PMCID: PMC11607083, DOI: 10.1038/s41419-024-07255-8.Peer-Reviewed Original ResearchConceptsActivation of focal adhesion kinaseExtracellular matrixActin-binding proteinsFocal adhesion kinaseLung extracellular matrixKnock-out mouse modelProtein kinase signalingCortical cytoskeletonLoss of ezrinKinase signalingPlasma membraneCell migrationSignaling pathwayEzrinResponse to lipopolysaccharideTissue-resident macrophagesMouse modelLipopolysaccharideCytoskeletonEzrin expressionLung microenvironmentKinaseMonocyte recruitmentProteinAktCellular stiffness sensing through talin 1 in tissue mechanical homeostasis
Chanduri M, Kumar A, Weiss D, Emuna N, Barsukov I, Shi M, Tanaka K, Wang X, Datye A, Kanyo J, Collin F, Lam T, Schwarz U, Bai S, Nottoli T, Goult B, Humphrey J, Schwartz M. Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis. Science Advances 2024, 10: eadi6286. PMID: 39167642, PMCID: PMC11338229, DOI: 10.1126/sciadv.adi6286.Peer-Reviewed Original ResearchConceptsTissue mechanical homeostasisStiffness sensingExtracellular matrixTalin-1Mechanical homeostasisExtracellular matrix mechanicsIncreased cell spreadingCell spreadingTalinMutationsCellular sensingFibrillar collagenReduced axial stiffnessTissue mechanical propertiesMechanical propertiesAxial stiffnessCompliant substratesHomeostasisRupture pressureArp2/3ARPC5LStiffnessHomeostasis hypothesisResident cellsTissue stiffnessIdentification of a fibronectin-binding protein signature associated with idiopathic pulmonary fibrosis
Sun Y, King B, Hamlin A, Saniepay M, Gorshkov K, Barker G, Ziegler M, Mukundan S, Cvijic M, Schwarzbauer J. Identification of a fibronectin-binding protein signature associated with idiopathic pulmonary fibrosis. Cells And Development 2024, 179: 203941. PMID: 39038657, PMCID: PMC11344656, DOI: 10.1016/j.cdev.2024.203941.Peer-Reviewed Original ResearchConceptsFn-binding proteinsExtracellular matrixInsulin-like growth factor binding protein-3Extracellular matrix protein fibronectinLevels of ECM proteinsExtracellular matrix proteinsIn vivo diseaseMatrisome genesEDB exonsProtein fibronectinECM proteinsRNA sequencingGene expressionTGF-bDown-regulationProteinHuman lung fibroblastsUp-regulatedLevels of FNProtein signaturesFibronectinFN expressionGrowth factor binding protein-3ExonAssociated with idiopathic pulmonary fibrosisSerologic extracellular matrix remodeling markers are related to fibrosis stage and prognosis in a phase 2b trial of simtuzumab in patients with primary sclerosing cholangitis
Thorburn D, Leeming D, Barchuk W, Wang Y, Lu X, Malkov V, Ito K, Bowlus C, Levy C, Goodman Z, Karsdal M, Muir A, Xu J. Serologic extracellular matrix remodeling markers are related to fibrosis stage and prognosis in a phase 2b trial of simtuzumab in patients with primary sclerosing cholangitis. Hepatology Communications 2024, 8: e0467. PMID: 38967589, PMCID: PMC11227354, DOI: 10.1097/hc9.0000000000000467.Peer-Reviewed Original ResearchConceptsPrimary sclerosing cholangitisELF scoreAdvanced fibrosisFibrosis stageFibrosis progressionClinical eventsSclerosing cholangitisExtracellular matrix remodeling markersRemodeling markersPredictors of disease severityPhase 2b trialBaseline to weekPRO-C3Progression to cirrhosisWilcoxon rank sum testMarker of collagen formationType III procollagenModerate diagnostic abilityDiagnosing fibrosis stagesRank sum testSerum markersNoninvasive predictorPrognostic markerModerate prognosisMedian changeTANGO1 inhibitors reduce collagen secretion and limit tissue scarring
Raote I, Rosendahl A, Häkkinen H, Vibe C, Küçükaylak I, Sawant M, Keufgens L, Frommelt P, Halwas K, Broadbent K, Cunquero M, Castro G, Villemeur M, Nüchel J, Bornikoel A, Dam B, Zirmire R, Kiran R, Carolis C, Andilla J, Loza-Alvarez P, Ruprecht V, Jamora C, Campelo F, Krüger M, Hammerschmidt M, Eckes B, Neundorf I, Krieg T, Malhotra V. TANGO1 inhibitors reduce collagen secretion and limit tissue scarring. Nature Communications 2024, 15: 3302. PMID: 38658535, PMCID: PMC11043333, DOI: 10.1038/s41467-024-47004-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCicatrixCollagenEndoplasmic ReticulumExtracellular MatrixFibroblastsFibrosisHumansPeptidesScleroderma, SystemicSkinWound HealingZebrafishConceptsEndoplasmic reticulum exit sitesECM proteinsSecretion of ECM proteinsPeptide inhibitorFibrotic diseasesCollagen exportTANGO1Binding interfaceWound healingZebrafish resultsECM componentsReduced granulation tissue formationGranulation tissue formationEffective therapyCutaneous wound healingInhibitor treatmentFibrotic processUncontrolled secretionWidespread occurrenceProtein levelsExit siteExcessive scarringAmount of collagenTherapeutic modulationTissue scarringBioengineering of vascularized porcine flaps using perfusion-recellularization
Xu M, D’Elia A, Hadzimustafic N, Adil A, Karoubi G, Waddell T, Haykal S. Bioengineering of vascularized porcine flaps using perfusion-recellularization. Scientific Reports 2024, 14: 7590. PMID: 38555385, PMCID: PMC10981729, DOI: 10.1038/s41598-024-58095-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBioengineeringBiomedical EngineeringExtracellular MatrixHumansPerfusionQuality of LifeSurgical FlapsSwineTissue EngineeringTissue ScaffoldsConceptsLow-concentration sodium dodecyl sulfateAcellular scaffoldsRecellularization techniquesPerfusion-decellularizationMesenchymal stromal cellsSodium dodecyl sulfateCell attachmentRecellularization approachCo-culture of human umbilical vein endothelial cellsRecellularizationHuman umbilical vein endothelial cellsReconstructive surgeryTensor fasciae lataeExtracellular matrixRepair optionsDonor site morbidityImpact patient qualityDodecyl sulfateClinically translatable optionTensor fascia lata flapUmbilical vein endothelial cellsSoft tissue defectsPorcine flapsMechanosensing regulates tissue repair program in macrophages
Meizlish M, Kimura Y, Pope S, Matta R, Kim C, Philip N, Meyaard L, Gonzalez A, Medzhitov R. Mechanosensing regulates tissue repair program in macrophages. Science Advances 2024, 10: eadk6906. PMID: 38478620, PMCID: PMC10936955, DOI: 10.1126/sciadv.adk6906.Peer-Reviewed Original ResearchConceptsExtracellular matrixRegulate chromatin accessibilityTissue repair programGene expression programsCytoskeletal dynamicsChromatin accessibilityAmoeboid migrationCytoskeletal remodelingBiochemical signalsTissue homeostasisExpression programsColony-stimulating factor-1Tissue-resident macrophagesFactor 1MechanosensingRegulating tissue repairTissue integrityMacrophagesTissueThree-dimensional environmentRepair programHomeostasisAltered extracellular matrix dynamics is associated with insulin resistance in adolescent children with obesity
Slusher A, Nouws J, Tokoglu F, Vash‐Margita A, Matthews M, Fitch M, Shankaran M, Hellerstein M, Caprio S. Altered extracellular matrix dynamics is associated with insulin resistance in adolescent children with obesity. Obesity 2024, 32: 593-602. PMID: 38410080, PMCID: PMC11034857, DOI: 10.1002/oby.23974.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentChildCollagenExtracellular MatrixHumansInsulinInsulin ResistanceIntra-Abdominal FatLipidsPediatric ObesitySubcutaneous FatConceptsSubcutaneous adipose tissueLiver fat contentInsulin resistanceInsulin sensitivityEctopic storage of lipidOral glucose tolerance testGluteal subcutaneous adipose tissueExtracellular matrix dynamicsMagnetic resonance imaging scansAdipose tissueBody fat distribution patternsFibrogenesis in vivoAdipocyte turnoverAssociated with insulin resistanceGlucose tolerance testFat distribution patternsWhole-body IRAdipose tissue insulin signalingTissue insulin signalingTolerance testGluteal fatImaging scansPathophysiological pathwaysAssociated with ratioObesityNovel muscle-derived extracellular matrix hydrogel promotes angiogenesis and neurogenesis in volumetric muscle loss
Chen Z, Huang Y, Xing H, Tseng T, Edelman H, Perry R, Kyriakides T. Novel muscle-derived extracellular matrix hydrogel promotes angiogenesis and neurogenesis in volumetric muscle loss. Matrix Biology 2024, 127: 38-47. PMID: 38325441, PMCID: PMC10958762, DOI: 10.1016/j.matbio.2024.02.001.Peer-Reviewed Original ResearchConceptsDecellularized extracellular matrixVolumetric muscle lossCharacterization of hydrogelsExtracellular matrix hydrogelTreat VML injuriesMuscle lossEnhancement of tissue repairRegenerative capacity of skeletal muscleMatrix hydrogelPromote muscle repairRegenerative capacityHydrogelsVolumetric muscle loss modelNative biomaterialsScar tissue formationEnhanced cell invasionCapacity of skeletal muscleMuscle repairTibialis anterior muscleClinical challengeBlood vessel formationReduce inflammationVML injuryLoss of functionSubcutaneous implantation
2023
A novel human iPSC model of COL4A1/A2 small vessel disease unveils a key pathogenic role of matrix metalloproteinases
Al-Thani M, Goodwin-Trotman M, Bell S, Patel K, Fleming L, Vilain C, Abramowicz M, Allan S, Wang T, Cader M, Horsburgh K, Van Agtmael T, Sinha S, Markus H, Granata A. A novel human iPSC model of COL4A1/A2 small vessel disease unveils a key pathogenic role of matrix metalloproteinases. Stem Cell Reports 2023, 18: 2386-2399. PMID: 37977146, PMCID: PMC10724071, DOI: 10.1016/j.stemcr.2023.10.014.Peer-Reviewed Original ResearchMeSH KeywordsBrainCollagen Type IVEndothelial CellsExtracellular MatrixHumansInduced Pluripotent Stem CellsMatrix MetalloproteinasesStrokeConceptsSmall vessel diseaseMatrix metalloproteinasesVessel diseaseCerebral small vessel diseaseKey pathogenic roleEndothelial cell tight junctionsHuman iPSC modelSVD pathologyCo-culture modelLeading causePathogenic roleCell phenotypic changesExtracellular matrixParacrine actionCell tight junctionsMMP activityTherapeutic opportunitiesECM abnormalitiesCell defectsEndothelial cellsMural cellsSmall vesselsTight junctionsIPSC modelsDiseaseFiber alignment in 3D collagen networks as a biophysical marker for cell contractility
Böhringer D, Bauer A, Moravec I, Bischof L, Kah D, Mark C, Grundy T, Görlach E, O'Neill G, Budday S, Strissel P, Strick R, Malandrino A, Gerum R, Mak M, Rausch M, Fabry B. Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility. Matrix Biology 2023, 124: 39-48. PMID: 37967726, PMCID: PMC10872942, DOI: 10.1016/j.matbio.2023.11.004.Peer-Reviewed Original ResearchConceptsCellular traction forcesTraction forceHepatic stellate cellsMatrix remodelingGlioblastoma cell linesFiber orientationCell contractilityDrug screening assaysLocal fiber orientationCell forcesRho-kinaseFiber alignmentStellate cellsCell linesScreening assayFiber networkCellsPython packageMechanical propertiesForce reconstructionBiopolymer matrixRemodelingInduced deformationQualitative proxyCollagen networkFibroblast activation protein: Pivoting cancer/chemotherapeutic insight towards heart failure
Gehris J, Ervin C, Hawkins C, Womack S, Churillo A, Doyle J, Sinusas A, Spinale F. Fibroblast activation protein: Pivoting cancer/chemotherapeutic insight towards heart failure. Biochemical Pharmacology 2023, 219: 115914. PMID: 37956895, PMCID: PMC10824141, DOI: 10.1016/j.bcp.2023.115914.Peer-Reviewed Original ResearchMeSH KeywordsExtracellular MatrixFibroblastsHeart FailureHumansMyocardiumNeoplasmsVentricular RemodelingConceptsFibroblast activation proteinExtracellular matrixCancer progressionFibroblast subtypesProtein signaturesECM structureEnzyme pathwaysActivation proteinTherapeutic targetImportant mechanismFibroblastsProliferationFibroblast growthProteinProgressionPathwayRemodelingFunctionCancerDiscoveryChemotherapeuticsSRC and TKS5 mediated podosome formation in fibroblasts promotes extracellular matrix invasion and pulmonary fibrosis
Barbayianni I, Kanellopoulou P, Fanidis D, Nastos D, Ntouskou E, Galaris A, Harokopos V, Hatzis P, Tsitoura E, Homer R, Kaminski N, Antoniou K, Crestani B, Tzouvelekis A, Aidinis V. SRC and TKS5 mediated podosome formation in fibroblasts promotes extracellular matrix invasion and pulmonary fibrosis. Nature Communications 2023, 14: 5882. PMID: 37735172, PMCID: PMC10514346, DOI: 10.1038/s41467-023-41614-x.Peer-Reviewed Original ResearchConceptsPulmonary fibrosisExtracellular matrix invasionLung fibroblastsIdiopathic pulmonary fibrosis patientsIdiopathic pulmonary fibrosisPulmonary fibrosis patientsMatrix invasionPromising therapeutic optionProfibrotic milieuTherapeutic optionsLung tissuePathogenic hallmarkPharmacological targetingFibrosisFibrosis patientsIncurable diseaseEx vivoBleomycinExtracellular matrix componentsTks5 expressionAberrant depositionInvasionMiceFibroblastsSrc kinaseCartilage-Related Collagens in Osteoarthritis and Rheumatoid Arthritis: From Pathogenesis to Therapeutics
Ouyang Z, Dong L, Yao F, Wang K, Chen Y, Li S, Zhou R, Zhao Y, Hu W. Cartilage-Related Collagens in Osteoarthritis and Rheumatoid Arthritis: From Pathogenesis to Therapeutics. International Journal Of Molecular Sciences 2023, 24: 9841. PMID: 37372989, PMCID: PMC10298547, DOI: 10.3390/ijms24129841.Peer-Reviewed Original ResearchMeSH KeywordsArthritis, RheumatoidCartilage, ArticularCollagenExtracellular MatrixHumansOsteoarthritisConceptsRheumatoid arthritisArticular cartilageCourse of osteoarthritisNew biochemical markersDisease progressionPathogenic factorsCartilage damageDegradation of collagenBiochemical markersProgressive destructionClinical diagnosisMechanical injuryConnective tissueDisease statesRole of collagenCollagen productionLow immunogenicityFacilitate drug developmentArthritisDrug developmentOsteoarthritisBiomechanical propertiesMechanical functionCartilageCollagen
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