2025
Evaluating the immunogenicity of a mouse partial hindlimb for composite allotransplantation
Sun J, Ligi S, Biniazan F, Haykal S. Evaluating the immunogenicity of a mouse partial hindlimb for composite allotransplantation. Frontiers In Immunology 2025, 16: 1595319. DOI: 10.3389/fimmu.2025.1595319.Peer-Reviewed Original ResearchPost-operative day 7CD8+ T cellsTr1 cellsVascularized composite allotransplantationAcute rejectionT cellsAllogeneic skinImmune responseTransplantation modelIncreased CD8+ T cellsImproving long-term graft survivalRecipient-derived T cellsFlow cytometryLong-term graft survivalPhenotype of infiltrating cellsImmune response complexityTolerance induction strategiesIncreased risk of infectionImmune cell populationsT cell presenceTUNEL-positive cellsInvestigate immune responsesSevere tissue traumaImmunosuppressive approachesSubclinical rejectionLKB1 regulates ILC3 postnatal development and effector function through metabolic programming
Zhang H, Zhao L, Zhang Q, Hu L, Su X, Sun J, Shen L. LKB1 regulates ILC3 postnatal development and effector function through metabolic programming. Frontiers In Immunology 2025, 16: 1587256. PMID: 40539052, PMCID: PMC12176730, DOI: 10.3389/fimmu.2025.1587256.Peer-Reviewed Original ResearchMeSH KeywordsAMP-Activated Protein Kinase KinasesAMP-Activated Protein KinasesAnimalsCitrobacter rodentiumEnterobacteriaceae InfectionsImmunity, InnateInterleukin-22InterleukinsIntestinal MucosaIntestinesLymphocytesMetabolic ReprogrammingMiceMice, Inbred C57BLMice, KnockoutProtein Serine-Threonine KinasesConceptsLiver kinase B1Intestinal immune homeostasisEffector functionsPostnatal developmentImmune homeostasisFlow cytometryGroup 3 innate lymphoid cellsMetabolic regulationIntestinal immunityLKB1 deficiencyIL-22 productionLKB1-deficient miceConditional knockout miceIntestinal inflammatory responsePotential therapeutic implicationsImpaired cell metabolismILC3 numbersIntestinal ILC3sLymphoid cellsILC3 functionCytokine productionILC3sKnockout miceMitochondrial massILC3 activationImmune biomarkers as predictors of response to mosunetuzumab in previously untreated follicular (FL) and marginal zone lymphoma (MZL).
Milrod C, Chorzalska A, Morgan J, Pardo M, Raker C, Ollila T, Pelcovits A, McMahon J, Donnelly S, Carmody C, Dallesandro F, Matasar M, Huntington S, Dubielecka P, Olszewski A. Immune biomarkers as predictors of response to mosunetuzumab in previously untreated follicular (FL) and marginal zone lymphoma (MZL). Journal Of Clinical Oncology 2025, 43: 7063-7063. DOI: 10.1200/jco.2025.43.16_suppl.7063.Peer-Reviewed Original ResearchMarginal zone lymphomaCTLA-4 levelsComplete responseT cell activationCTLA-4T cellsImmune biomarkersNK cellsCytokine levelsInvestigator-initiated phase 2 trialMarkers of T-cell activationEffector memory T-cell subsetsIndolent B-cell lymphomaCD8+ T cellsMemory T cell subsetsInvestigation of combination therapyFlow cytometryCD8+ subsetsBaseline cytokine levelsCirculating NK cellsB-cell lymphomaT cell subsetsImmune cell subsetsMultiplex Luminex assaySystemic immune changesImpact of peritoneal macrophage depletion on endometriotic lesion development in a mouse model
Stratopoulou C, Cacciottola L, Gawde N, Ngombo A, Brusa D, Donnez J, Taylor H, Dolmans M. Impact of peritoneal macrophage depletion on endometriotic lesion development in a mouse model. Journal Of Endometriosis And Uterine Disorders 2025, 10: 100113. DOI: 10.1016/j.jeud.2025.100113.Peer-Reviewed Original ResearchMacrophage depletionEndometriotic lesionsTreated miceMacrophage accumulationMouse modelVascular endothelial growth factor immunostainingEndometriosis developmentQuantification of immune cellsFlow cytometryMouse model of endometriosisClodronate-containing liposomesKi67-positive proliferating cellsNovel therapeutic optionsRate of fibrosisModel of endometriosisTUNEL-positive apoptotic cellsLevels of fibrosisPathogenesis of endometriosisReproductive-age womenMasson's trichrome stainingEndometriotic lesion developmentEnhanced cell deathPlacebo-controlledUterine fragmentsT cellsPartial Hydatidiform Mole
Buza N. Partial Hydatidiform Mole. 2025, 83-96. DOI: 10.1007/978-3-031-83545-2_6.Peer-Reviewed Original ResearchPartial hydatidiform moleGestational trophoblastic neoplasiaGestational trophoblastic diseaseHydatidiform moleComplete moleTrophoblastic hyperplasiaPersistent gestational trophoblastic diseaseNon-neoplastic proliferationTriploid gestationsTrophoblastic neoplasiaCytogenetic abnormalitiesTrophoblastic diseaseClinical presentationVillous trophoblastDiagnostic accuracyHydatidiformFlow cytometryTriploid karyotypeHyperplasiaFocal sparingVilliGestationFetusesEmbryo/fetusNeoplasiaTriple negative breast cancer cells acquire lymphocyte proteins and genomic DNA during trogocytosis with T cells
Sivakoses A, Marcarian H, Arias A, Lam A, Ihedioha O, Santamaria-Barria J, Gurtner G, Bothwell A. Triple negative breast cancer cells acquire lymphocyte proteins and genomic DNA during trogocytosis with T cells. PeerJ 2025, 13: e19236. PMID: 40183054, PMCID: PMC11967428, DOI: 10.7717/peerj.19236.Peer-Reviewed Original ResearchConceptsTriple negative breast cancerTriple negative breast cancer cellsT cellsCancer cellsTumor cell expressionNegative breast cancer cellsNegative breast cancerGenomic DNABreast cancer cellsCluster of differentiationSpecific proteinsT cell-specific proteinsLymphoid markersBreast cancerDonor cellsCell expressionCD45Immune detectionFlow cytometryPatient samplesTrogocytosisCD45RACo-cultureImmunofluorescence imagingLymphocyte proteinIn vivo synergistic enhancement of MIF‐mediated inflammation in acute lung injury by the plant ortholog Arabidopsis MDL1
Spiller L, Zhang L, Gerra S, Stoppe C, Scheiermann P, Calandra T, Lolis E, Panstruga R, Bernhagen J, Hoffmann A. In vivo synergistic enhancement of MIF‐mediated inflammation in acute lung injury by the plant ortholog Arabidopsis MDL1. The FASEB Journal 2025, 39: e70489. PMID: 40134325, PMCID: PMC11937861, DOI: 10.1096/fj.202403301r.Peer-Reviewed Original ResearchConceptsAcute lung injuryLung injuryPulmonary infiltration of neutrophilsAdministered to C57BL/6 miceAcute respiratory distress syndromeParameters of lung injuryRespiratory distress syndromeInfiltration of neutrophilsPro-inflammatory cytokine genesPulmonary inflammationDistress syndromeC57BL/6 miceLeukocyte infiltrationArabidopsis thaliana proteinsInflammatory mediatorsHuman MIFMouse modelCytokine genesCombined treatmentFlow cytometryMonocytic cellsInflammationImmunofluorescence microscopyMIFInjuryInitial management of patients with acquired aplastic anemia in the United States: results from a large national claims database
Stempel J, Wang R, Lee A, Zeidan A, Ma X, Podoltsev N. Initial management of patients with acquired aplastic anemia in the United States: results from a large national claims database. Annals Of Hematology 2025, 104: 2507-2515. PMID: 40102305, PMCID: PMC12052795, DOI: 10.1007/s00277-025-06307-z.Peer-Reviewed Original ResearchConceptsHematopoietic stem cell transplantationAcquired aplastic anemiaCalcineurin inhibitorsParoxysmal nocturnal hemoglobinuriaTransfusion-dependentTriple therapyAA patientsAplastic anemiaAllogeneic hematopoietic stem cell transplantationTreated with calcineurin inhibitorsTreated with antithymocyte globulinFlow cytometryInitial management of patientsSevere AA patientsStem cell transplantationBone marrow failureImmune-mediated disordersTreatment initiation delayRetrospective cohort studyManagement of patientsDiagnosis to treatmentNational claims databaseAntithymocyte globulinSibling donorMarrow failureDEVELOPMENT OF EX VIVO PATIENT-DERIVED MODELS TO UNCOVER THE TUMOR-IMMUNE MICROENVIRONMENT IN RENAL CELL CARCINOMA
Kashima S, Gupta R, Moritz V, Sadak K, Walker J, Adeniran A, Humphrey P, Dinulescu D, Palmer D, Hammond S, Bosenberg M, Hurwitz M, Kenney P, Braun D. DEVELOPMENT OF EX VIVO PATIENT-DERIVED MODELS TO UNCOVER THE TUMOR-IMMUNE MICROENVIRONMENT IN RENAL CELL CARCINOMA. Urologic Oncology Seminars And Original Investigations 2025, 43: 22-23. DOI: 10.1016/j.urolonc.2024.12.057.Peer-Reviewed Original ResearchRCC tumor microenvironmentPatient-derived tumor modelsRenal cell carcinomaImmune checkpoint inhibitorsT cell functionTumor microenvironmentT cellsEnzyme-linked immunosorbent assayFlow cytometryTumor fragmentsTumor samplesCytokine productionImpact of immune checkpoint inhibitorsAnti-PD-1 monoclonal antibodyCD4+CD25+ regulatory T cellsResection of renal cell carcinomaSurgical resection of renal cell carcinomaCD8+ T cell populationsAnti-PD-1 antibodyMetastatic renal cell carcinomaCD20+ B cellsActivated CD8 T cellsEvaluate T cell activationT cell cytokine productionStudy of renal cell carcinomaAssociation of localized high-risk prostate cancer (PC) and an androgen receptor low subpopulation susceptible to HER2 inhibition.
Sowalsky A, Wilkinson S, Ku A, Lis R, King I, Low D, Trostel S, Baj A, Kartal S, Heyward K, Vo B, Jansen C, Ye H, Harmon S, Kissick H, Pinto P, Choyke P, Turkbey B, Dahut W, Karzai F. Association of localized high-risk prostate cancer (PC) and an androgen receptor low subpopulation susceptible to HER2 inhibition. Journal Of Clinical Oncology 2025, 43: 401-401. DOI: 10.1200/jco.2025.43.5_suppl.401.Peer-Reviewed Original ResearchAndrogen deprivation therapyPathological responseHER2 activationRadical prostatectomyAR activityProstate tumorsHER2 inhibitionProstate cancerMultiplex immunofluorescenceAndrogen receptorMonths prior to radical prostatectomyLocalized high-risk prostate cancerHigh-risk prostate cancerInitiation of neoadjuvant therapyNeoadjuvant androgen deprivation therapyHER2 inhibitorsFlow cytometryTreated with low dosesAssociated with poor outcomesHigh-risk PCIntensive hormone therapyOccult micrometastatic diseasePoor pathologic responseResidual cancer volumeHuman prostate tumorsDecreased T helper 1 cell function underlies recurrent sinopulmonary infections in the 17q12 deletion syndrome
Shin J, Shin H, Gutierrez A, Yoo N, Par-Young J, Osmani L, Shin M, Sanchez-Lara P, Bucala R, Soffer G, Kang I. Decreased T helper 1 cell function underlies recurrent sinopulmonary infections in the 17q12 deletion syndrome. EBioMedicine 2025, 112: 105578. PMID: 39891996, PMCID: PMC11840234, DOI: 10.1016/j.ebiom.2025.105578.Peer-Reviewed Original ResearchConceptsCD4<sup>+</sup> T cellsRecurrent sinopulmonary infectionsT cell functionRNA-seq analysisT cellsHealthy controlsSinopulmonary infectionsRNA-seqT-betIFN-gFrequency of CD4<sup>+</sup> T cellsCD4<sup>+</sup> T cell functionTh1 transcription factor T-betDeletion syndromeFlow cytometryCompared to age-matched healthy controlsTranscription factor T-betDecreased T-betUrinary tract abnormalitiesAge-matched healthy controlsMultiplex assayDownstream effector cytokinesEffector cytokinesRecurrent infectionsTh17 cytokinesCrosstalk Between nNOS/NO and COX-2 Enhances Interferon-Gamma-Stimulated Melanoma Progression
Patel A, Tong S, Roosan M, Syed B, Awasthi A, Silverman R, Yang S. Crosstalk Between nNOS/NO and COX-2 Enhances Interferon-Gamma-Stimulated Melanoma Progression. Cancers 2025, 17: 477. PMID: 39941844, PMCID: PMC11816268, DOI: 10.3390/cancers17030477.Peer-Reviewed Original ResearchNeuronal nitric oxide synthaseIFN-gPro-tumorigenic activityCOX-2Human melanoma xenograft mouse modelMelanoma progressionMelanoma cellsFlow cytometryInduction of neuronal nitric oxide synthaseNeuronal nitric oxide synthase blockadeIn vivo antitumor efficacyMelanoma xenograft mouse modelMelanoma tumor microenvironmentLevels of PGE<sub>2</sub>PD-L1 expressionAnticancer immune responseMelanoma tumor growth in vivoCOX-2 expression levelsAnalysis of patientsInduction of COX-2Increased intracellular NO levelsSTAT3 inhibitor NapabucasinNitric oxideInhibited COX-2 expressionXenograft mouse modelP-531. Mitochondrial DNA Haplogroups and Circulating Immune Cell Phenotypes in Veterans with and without HIV
Yan A, Kundu S, Samuels D, Bailin S, Xu K, So-Armah K, Butt A, Gerschenson M, Goetz M, Tracy R, Marconi V, Justice A, Freiberg M, Koethe J, Hulgan T. P-531. Mitochondrial DNA Haplogroups and Circulating Immune Cell Phenotypes in Veterans with and without HIV. Open Forum Infectious Diseases 2025, 12: ofae631.730. PMCID: PMC11777815, DOI: 10.1093/ofid/ofae631.730.Peer-Reviewed Original ResearchCD8+ T cellsPeripheral blood mononuclear cellsCD4+ T cellsT cellsHIV statusEffector memory CD8+ T cellsPeripheral blood mononuclear cell phenotypeMemory CD8+ T cellsCirculating immune cell phenotypesFlow cytometryPlasma viral loadImmune response to viral infectionImmune cell phenotypesBlood mononuclear cellsCellular immune responsesInfluence cellular immune responsesWilcoxon rank sum testResponse to viral infectionSignificant univariate associationsVeterans Aging Cohort Study Biomarker CohortRank sum testBlack PWHCD28-negativeMemory CD8White PWH
2024
A novel sensitizer reduces EGFR-TKI resistance by regulating the PI3K/Akt/mTOR pathway and autophagy
Zhang J, Qu Z, Xiao X, Adelson D, Wang F, Wei A, Harata-Lee Y, Cui J, He D, Xie L, Sun L, Li J, Huang Z, Aung T, Yao H, Lin L. A novel sensitizer reduces EGFR-TKI resistance by regulating the PI3K/Akt/mTOR pathway and autophagy. Heliyon 2024, 11: e41104. PMID: 39844968, PMCID: PMC11750466, DOI: 10.1016/j.heliyon.2024.e41104.Peer-Reviewed Original ResearchEpidermal growth factor receptor tyrosine kinase inhibitorsEGFR-TKI-resistant cell linesNon-small cell lung cancerInhibition of drug resistanceLung cancerEpidermal growth factor receptor tyrosine kinase inhibitor resistanceDrug resistanceGrowth factor receptor tyrosine kinase inhibitorsCell linesReceptor tyrosine kinase inhibitorsEGFR-TKI resistanceResistance to gefitinibCell lung cancerFirst-line treatmentPI3K/AKT/mTOR pathwayMortality of lung cancerEGFR mutationsTreatment failureMolecular mechanismsDose-dependentlyKinase inhibitorsFlow cytometryAnticancer effectsGefitinibH1650 cellsEndometrial stromal cell signaling and microRNA exosome content in women with adenomyosis
Zipponi M, Cacciottola L, Camboni A, Stratopoulou C, Taylor H, Dolmans M. Endometrial stromal cell signaling and microRNA exosome content in women with adenomyosis. Molecular Human Reproduction 2024, 31: gaae044. PMID: 39673794, DOI: 10.1093/molehr/gaae044.Peer-Reviewed Original ResearchStromal cell signalsMenstrual phaseHealthy subjectsPresence of endometrial glandsPathogenesis of adenomyosisCompared to controlsEndometrial biopsyEndometrial glandsEutopic endometriumStromal compartmentAdenomyosisAdenomyosis developmentMolecular mechanismsCell signalingLesion establishmentStromal cellsNanoparticle tracking analysisFlow cytometryEpithelial glandsParacrine signalingQuality of lifeSecreted exosomesIntercellular communicationExosomal contentsReproductive healthA Phase III, Randomized Study of Daratumumab, Cyclophosphamide, Bortezomib and Dexamethasone (DARA-VCD) Induction Followed By Autologous Stem Cell Transplant or Dara-VCD Consolidation and Daratumumab Maintenance in Patients with Newly Diagnosed AL Amyloidosis
Hagen P, Sidana S, Parker T, Walker B, Sanchorawala V, Zonder J, Kourelis T, D'Souza A, Landau H, Rosenthal A, Hoering A, Ailawadhi S, Orlowski R. A Phase III, Randomized Study of Daratumumab, Cyclophosphamide, Bortezomib and Dexamethasone (DARA-VCD) Induction Followed By Autologous Stem Cell Transplant or Dara-VCD Consolidation and Daratumumab Maintenance in Patients with Newly Diagnosed AL Amyloidosis. Blood 2024, 144: 3309.1-3309.1. DOI: 10.1182/blood-2024-208227.Peer-Reviewed Original ResearchAutologous stem cell transplantationNext generation flow cytometryStem cell transplantationOverall survivalConsolidation therapyAL amyloidCell transplantationASCT armLight chain (AL) amyloidosisSeverity of cardiac involvementFlow cytometryAL amyloid patientsDaratumumab to bortezomibTransplant related mortalityProgression free survivalHematologic response rateTwo-sided significance levelPlasma cell malignancyMulti-organ involvementDaratumumab maintenanceInduction therapyMRD negativityFree survivalIntergroup trialCardiac involvementAddition of Blinatumomab to Consolidation Therapy Among Older Newly Diagnosed Patients (pts) with BCR::ABL1 Negative B-Lineage Acute Lymphoblastic Leukemia (ALL) in the ECOG-ACRIN E1910 Randomized Phase III Trial
Podoltsev N, Sun Z, Litzow M, Paietta E, Roberts K, Zhang Y, Racevskis J, Lazarus H, Rowe J, Arber D, Wieduwilt M, Liedtke M, Bergeron J, Wood B, Zhao Y, Wu G, Chang T, Zhang W, Pratz K, Dinner S, Frey N, Gore S, Bhatnagar B, Atallah E, Uy G, Jeyakumar D, Lin T, Willman C, DeAngelo D, Patel S, Elliott M, Advani A, Tzachanis D, Vachhani P, Roy R, Sharon E, Little R, Erba H, Stone R, Mullighan C, Tallman M, Luger S, Mattison R. Addition of Blinatumomab to Consolidation Therapy Among Older Newly Diagnosed Patients (pts) with BCR::ABL1 Negative B-Lineage Acute Lymphoblastic Leukemia (ALL) in the ECOG-ACRIN E1910 Randomized Phase III Trial. Blood 2024, 144: 4211-4211. DOI: 10.1182/blood-2024-207143.Peer-Reviewed Original ResearchHematopoietic stem cell transplantationRelapse free survivalAcute lymphoblastic leukemiaWhite blood cell countOlder ptsOverall survivalBone marrowAllogeneic hematopoietic stem cell transplantationB-lineage acute lymphoblastic leukemiaRandomized phase III trialFlow cytometryMulti-color flow cytometryStratified multivariable Cox modelsIncreased treatment toxicityPeripheral blood blastsStratified log-rank testImprovement of OSHigh dose methotrexateImproved overall survivalMedian follow-upStem cell transplantationKaplan-Meier methodPhase III trialsEstimates of OSLog-rank testFlow cytometry for RBC damage and complement activation
Villalba C, Yurtsever N, Paternoster K, Gallipoli P, Nash B, Bizzario L, Shah B, Rinder H, Tormey C, Lee E. Flow cytometry for RBC damage and complement activation. American Journal Of Clinical Pathology 2024, 162: s182-s183. DOI: 10.1093/ajcp/aqae129.400.Peer-Reviewed Original ResearchDirect antiglobulin testRed blood cellsTukey HSD testHSD testAntiglobulin testComplement depositionOne-way ANOVANegative direct antiglobulin testFlow cytometryRed blood cell clearanceStatistically significant differenceTwo-sample t-testPercentage of red blood cellsExposure of PSHealthy donorsDiluted red blood cellsRBC recoveryRBC unitsPhosphatidylserine expressionRBC injuryFlow cytometry assayHealthy individualsPS expressionRed blood cell ageComplement activationPhysalia gonodendra are not yet sexually mature when released
Oguchi K, Yamamoto G, Kohtsuka H, Dunn C. Physalia gonodendra are not yet sexually mature when released. Scientific Reports 2024, 14: 23011. PMID: 39362967, PMCID: PMC11450099, DOI: 10.1038/s41598-024-73611-5.Peer-Reviewed Original ResearchConceptsGerm cell maturationGene expression analysisGerm cell markersReproductive ecologySexual reproductionHaploid cellsGerm cellsReproductive biologyGonophoresExpression analysisRelated genesLife cycleGenesCell maturationGermConsistent with other studiesFlow cytometryPhysalia utriculusExpressionCnidariaCell markersCellsPhysaliaSpermHydrozoaVγ6/Vδ1+ γδ T cells protect from angiotensin II effects on blood pressure and endothelial function in mice
Mahmoud A, Caillon A, Shokoples B, Ferreira N, Comeau K, Hatano S, Yoshikai Y, Lewis J, Tigelaar R, Paradis P, Schiffrin E. Vγ6/Vδ1+ γδ T cells protect from angiotensin II effects on blood pressure and endothelial function in mice. Journal Of Hypertension 2024, 43: 109-119. PMID: 39248136, DOI: 10.1097/hjh.0000000000003871.Peer-Reviewed Original ResearchGd T cellsT cellsBlood pressureAngII infusionEndothelial dysfunctionBP elevationPerivascular tissueAngiotensin II (AngII)-induced hypertensionSpecific T cell receptorMesenteric arterial functionArtery endothelial dysfunctionAngiotensin II effectsDescending thoracic aortaT cell receptorAngII-infused micePerivascular adipose tissuePressure myographyHypertensive effectEndothelial functionThoracic aortaII effectsMale miceVascular injuryArterial functionFlow cytometry
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