2025
YAP controls cell migration and invasion through a Rho GTPase switch
Shah S, Ren C, Tippens N, Park J, Mohyeldin A, Wang S, Vela G, Martinez-Gutierrez J, Margolis S, Schmidt S, Quiñones-Hinojosa A, Levchenko A. YAP controls cell migration and invasion through a Rho GTPase switch. Science Signaling 2025, 18: eadu3794. PMID: 40424361, DOI: 10.1126/scisignal.adu3794.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBrain NeoplasmsCell Line, TumorCell MovementFemaleGlioblastomaGuanine Nucleotide Exchange FactorsHumansMiceNeoplasm InvasivenessPhosphoproteinsProtein Serine-Threonine Kinasesrac1 GTP-Binding ProteinrhoA GTP-Binding ProteinSignal TransductionSTAT3 Transcription FactorTranscription FactorsYAP-Signaling ProteinsConceptsCell migrationGuanine nucleotide exchange factor TrioRegulation of cytoskeletal dynamicsRho family guanosine triphosphatasesInvasive cell spreadTranscriptional coactivator YAPActivation of Rac1Inhibition of RhoAHyperactivation of YAPHuman breast epithelial cellsIncreased cell migrationBreast epithelial cellsGTPase switchAssociated with cancer metastasisMovement of cellsCytoskeletal dynamicsGuanosine triphosphataseSignaling networksInvasion in vitroIntronic enhancerTranscription factorsCell spreadingRac1Invasive behaviorPathological contextsSugar unmasking for trafficking
Calderwood D, Toomre D. Sugar unmasking for trafficking. Nature Cell Biology 2025, 27: 375-376. PMID: 40038537, DOI: 10.1038/s41556-025-01615-y.Peer-Reviewed Original ResearchGeneration of Orthogonal Gradients of the Matrix Stiffness and Chemotactic Cues in a Suspended Array of Hydrogel to Study hMSCs Migration
Xu Z, Ponek A, Thomas J, Qyang Y. Generation of Orthogonal Gradients of the Matrix Stiffness and Chemotactic Cues in a Suspended Array of Hydrogel to Study hMSCs Migration. ACS Sensors 2025, 10: 1722-1728. PMID: 40021359, DOI: 10.1021/acssensors.4c02793.Peer-Reviewed Original ResearchConceptsMatrix stiffnessChemotactic cuesHuman mesenchymal stem cellsHydrogel cylindersCell migrationDevice working principleMigration of human mesenchymal stem cellsFactor 1 alphaCellular contextOrthogonal gradientsReplica moldingGlass substratesCell migration studiesDevice fabricationWorking principleMicrofluidic devicesIn vitro assaysHydrogelsStiffnessCell typesSuspended arrayMesenchymal stem cellsConcentration gradientPoly(dimethylsiloxaneCylinderOncostatin M modulates the biology of cholangiocarcinoma cells and the tumor microenvironment
Porro N, Nurcis J, De Siervi S, Cadamuro M, Turato C, Mantovani S, Oliviero B, Fabris L, Mondelli M, Marra F, Parola M, Caligiuri A, Pastore M, Cannito S, Gentilini A. Oncostatin M modulates the biology of cholangiocarcinoma cells and the tumor microenvironment. Digestive And Liver Disease 2025, 57: s18. DOI: 10.1016/j.dld.2025.01.031.Peer-Reviewed Original ResearchTumor microenvironmentOncostatin MStromal cellsFibrotic tumor microenvironmentPrimary hepatic stellate cellsCultured stromal cellsHuman iCCA cell linesResistance to chemotherapyCell migrationTumor-stroma interactionsDose-dependent increaseExpression of oncostatin MCancer-associated fibroblastsHepatic stellate cellsOncostatin M receptorAggressive tumorsInvasion of iCCA cellsHuman CCA specimensPoor prognosisPeritumoral tissuesCancer-associated pathwaysIncreased cell migrationConditioned mediumTumorCancer cellsG3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration
Boraas L, Hu M, Martino P, Thornton L, Vejnar C, Zhen G, Zeng L, Parker D, Cox A, Giraldez A, Su X, Mayr C, Wang S, Nicoli S. G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration. Cell Reports 2025, 44: 115237. PMID: 39883578, PMCID: PMC11923778, DOI: 10.1016/j.celrep.2025.115237.Peer-Reviewed Original ResearchConceptsRNA-binding proteinsFocal adhesionsCell migrationStress granulesRNA-dependent mannerProtein mobilityFA proteinsRNA bindingDimerization domainSubcellular localizationRibonucleoprotein complexNon-stress conditionsFA sizeCell speedG3BP1RibonucleoproteinFA localizationBiological processesB-actinMRNAProteinCellsFA functionMigrationLocalization
2024
Ezrin drives adaptation of monocytes to the inflamed lung microenvironment
Gudneppanavar R, Di Pietro C, H Öz H, Zhang P, Cheng E, Huang P, Tebaldi T, Biancon G, Halene S, Hoppe A, Kim C, Gonzalez A, Krause D, Egan M, Gupta N, Murray T, Bruscia E. Ezrin drives adaptation of monocytes to the inflamed lung microenvironment. Cell Death & Disease 2024, 15: 864. PMID: 39613751, PMCID: PMC11607083, DOI: 10.1038/s41419-024-07255-8.Peer-Reviewed Original ResearchConceptsActivation of focal adhesion kinaseExtracellular matrixActin-binding proteinsFocal adhesion kinaseLung extracellular matrixKnock-out mouse modelProtein kinase signalingCortical cytoskeletonLoss of ezrinKinase signalingPlasma membraneCell migrationSignaling pathwayEzrinResponse to lipopolysaccharideTissue-resident macrophagesMouse modelLipopolysaccharideCytoskeletonEzrin expressionLung microenvironmentKinaseMonocyte recruitmentProteinAktCofilin-Mediated Filament Softening and Crosslinking Counterbalance to Enhance Actin Network Flexibility
Sun Z, Murrell M. Cofilin-Mediated Filament Softening and Crosslinking Counterbalance to Enhance Actin Network Flexibility. Physical Review Letters 2024, 133: 218402. PMID: 39642486, DOI: 10.1103/physrevlett.133.218402.Peer-Reviewed Original ResearchActin-binding proteinsF-actin networkF-actinCrosslinking proteinsF-actin crosslinking proteinCrosslinks F-actinF-actin filamentsTransmission of mechanical forcesCofilin concentrationFilamentous-actinAccessory proteinsCell cytoskeletonCell divisionCell shapeCofilinBinding proteinCell migrationFilament flexibilityProteinDisulfide bondsFilament levelFilamentsCellsMechanical forcesLow pHViscosity regulates cell spreading and cell‐extracellular matrix interactions
Xiao H, Gong X, Jordan S, Liang Z, Mak M. Viscosity regulates cell spreading and cell‐extracellular matrix interactions. The FEBS Journal 2024, 292: 740-758. PMID: 39529371, PMCID: PMC12002552, DOI: 10.1111/febs.17306.Peer-Reviewed Original ResearchCell spreadingRho-associated protein kinase 1Actin-related protein 2/3Regulation of cell locomotionRegulation of ECM remodelingCollagen substrateRas-related C3 botulinum toxin substrate 1Cell-extracellular matrix interactionsECM remodelingCellular remodelingExtracellular matrixEnhanced cell spreadingProtein kinase 1Membrane rufflingCell locomotionRemodeling of extracellular matrixCellular forcesSubstrate 1Cell migrationCellular spreadingKinase 1Matrix interactionsRac1MicrotubulesRegulationPhysics of bacterial chemotaxis
Moore J, Emonet T. Physics of bacterial chemotaxis. Current Biology 2024, 34: r972-r977. PMID: 39437738, DOI: 10.1016/j.cub.2024.05.054.Peer-Reviewed Original ResearchCRISPR-based dissection of microRNA-23a ~ 27a ~ 24-2 cluster functionality in hepatocellular carcinoma
Cui M, Liu Z, Wang S, Bae S, Guo H, Zhou J, Liu R, Wang L. CRISPR-based dissection of microRNA-23a ~ 27a ~ 24-2 cluster functionality in hepatocellular carcinoma. Oncogene 2024, 43: 2708-2721. PMID: 39112518, PMCID: PMC11364504, DOI: 10.1038/s41388-024-03115-z.Peer-Reviewed Original ResearchConceptsMiR-23aMiR-27aCRISPR interferenceCRISPR activationHigh-throughput RNA-seqCell migrationCDK1/cyclin B activityReduced cell growth in vitroMiRNA target predictionCell cycle arrestMiRNA clusterHepatocellular carcinoma cellsCell growth in vitroRNA-seqGene networksTarget predictionCRISPR knockoutOncogenic roleGrowth in vitroCycle arrestMature miRNAsMiRNAsG2/M phaseSignaling pathwayOncogenic functionActive tension and membrane friction mediate cortical flows and blebbing in a model actomyosin cortex
Sakamoto R, Murrell M. Active tension and membrane friction mediate cortical flows and blebbing in a model actomyosin cortex. Physical Review Research 2024, 6: 033024. DOI: 10.1103/physrevresearch.6.033024.Peer-Reviewed Original ResearchActomyosin cortexCell membraneActin cytoskeletonCortical flowMembrane blebbingCell divisionCell migrationCytoskeletonActomyosinBiological phenomenaMembrane bulgesBlebsCellsMembraneViscoelastic fluidMechanical responseElastic stressesStress yieldActinUbiquitous structuresApoptosisMechanical stressMembrane elasticityPhysical behaviormiR‐1246 promotes osteosarcoma cell migration via NamiRNA‐enhancer network dependent on Argonaute 2
Yang S, Zou Q, Liang Y, Zhang D, Peng L, Li W, Li W, Liu M, Tong Y, Chen L, Xu P, Yang Z, Zhou K, Xiao J, Wang H, Yu W. miR‐1246 promotes osteosarcoma cell migration via NamiRNA‐enhancer network dependent on Argonaute 2. MedComm 2024, 5: e543. PMID: 38585233, PMCID: PMC10999177, DOI: 10.1002/mco2.543.Peer-Reviewed Original ResearchArgonaute 2Gene activationMiR-1246Gene expressionCell migrationActivate gene expressionNuclear activating miRNAsActivate gene transcriptionOsteosarcoma cellsOsteosarcoma metastasisOsteosarcoma cell migrationMNNG cellsPAZ domainEnhancer DNAH3K27ac enrichmentMiR-1246 levelActive miRNAsGene transcriptionRNase HMalignant osteosarcoma cellsIn vitro assaysPromote tumor metastasisNamiRNAsArgonauteExtracellular vesiclesMLL1 regulates cytokine-driven cell migration and metastasis
Nair P, Danilova L, Gómez-de-Mariscal E, Kim D, Fan R, Muñoz-Barrutia A, Fertig E, Wirtz D. MLL1 regulates cytokine-driven cell migration and metastasis. Science Advances 2024, 10: eadk0785. PMID: 38478601, PMCID: PMC10936879, DOI: 10.1126/sciadv.adk0785.Peer-Reviewed Original ResearchConceptsMethyltransferase mixed-lineage leukemia 1Cell migrationControls actin filament assemblyRegulation of cell migrationHistone methyltransferase mixed-lineage leukemia 1Actin filament assemblyCell cycle-related pathwaysCancer cell migrationMixed-lineage leukemia 1Regulating cell proliferationMyosin contractilityFilament assemblyProtein meninAssociated with immune cellsMetastatic burdenCancer cellsCell proliferationPrimary tumor growth rateLung metastatic burdenTumor growth rateGrowth rateCellsPreexisting metastasesMetastatic diseaseTumor growthStem cell migration drives lung repair in living mice
Chioccioli M, Liu S, Magruder S, Tata A, Borriello L, McDonough J, Konkimalla A, Kim S, Nouws J, Gonzalez D, Traub B, Ye X, Yang T, Entenberg D, Krishnaswamy S, Hendry C, Kaminski N, Tata P, Sauler M. Stem cell migration drives lung repair in living mice. Developmental Cell 2024, 59: 830-840.e4. PMID: 38377991, PMCID: PMC11003834, DOI: 10.1016/j.devcel.2024.02.003.Peer-Reviewed Original ResearchStem cell migrationCell migrationAlveolar type 2 cellsAlveolar unitsStem cell motilityAlveolar type 1 cellsStem cell activityCellular response to injuryResponse to injuryType 2 cellsMotile phenotypeType 1 cellsCell motilityLung repairImpaired regenerationGenetic depletionCell activationAT2Stem cellsTissue repairAT1Longitudinal imagingInjuryMotilityCellular resolutionHox Genes
Duraiswamy A, Senkumar L, De Kumar B. Hox Genes. 2024 DOI: 10.1016/b978-0-12-822563-9.00196-7.Peer-Reviewed Original ResearchHox genesAberrant expression of HOX genesCell fate determinationExpression of Hox genesFate determinationTissue homeostasisMetastatic behavior of tumorsCell migrationGenesDevelopmental eventsDevelopmental defectsHoxCancer progressionAberrant expressionMetastatic behaviorBehavior of tumorsCellsDisease progressionDisease prognosisProteinHomeostasisOrganogenesisPrognosis
2023
Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton
Amiri S, Muresan C, Shang X, Huet-Calderwood C, Schwartz M, Calderwood D, Murrell M. Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton. Nature Communications 2023, 14: 8011. PMID: 38049429, PMCID: PMC10695988, DOI: 10.1038/s41467-023-43612-5.Peer-Reviewed Original ResearchConceptsF-actin architectureStress fibersCortical actinActin cytoskeletonMolecular tension sensorsF-actin stress fibersF-actin cytoskeletonFilamentous actin cytoskeletonMechanical forcesTension sensorCell divisionCytoskeletonCell migrationExtracellular matrixMyosin inhibitionActinDirection of stretchCellsCell axisUniaxial stretchStretchFRETAbl2 repairs microtubules and phase separates with tubulin to promote microtubule nucleation
Duan D, Lyu W, Chai P, Ma S, Wu K, Wu C, Xiong Y, Sestan N, Zhang K, Koleske A. Abl2 repairs microtubules and phase separates with tubulin to promote microtubule nucleation. Current Biology 2023, 33: 4582-4598.e10. PMID: 37858340, PMCID: PMC10877310, DOI: 10.1016/j.cub.2023.09.018.Peer-Reviewed Original ResearchConceptsCOS-7 cellsMT nucleationMT latticeFamily kinasesTubulin recruitmentLiquid-liquid phase separationTubulin C-terminal tailsCryo-EM analysisC-terminal tailAbl family kinasesWild-type cellsC-terminal halfRescue frequencyGenetic experimentsNeuronal morphogenesisMicrotubule nucleationSplice isoformsMicrotubule dynamicsNocodazole treatmentMolecular mechanismsAxon guidanceCell migrationDamage sitesABL2MT assemblyMembrane tension induces F-actin reorganization and flow in a biomimetic model cortex
Sakamoto R, Banerjee D, Yadav V, Chen S, Gardel M, Sykes C, Banerjee S, Murrell M. Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex. Communications Biology 2023, 6: 325. PMID: 36973388, PMCID: PMC10043271, DOI: 10.1038/s42003-023-04684-7.Peer-Reviewed Original ResearchConceptsF-actin reorganizationMechanical stressCell cortexBiochemical regulationCell shapeActomyosin cortexPhysical behaviorCell polarizationMembrane tensionCell migrationEssential physical behaviorPore openingSpatial assemblyDrive changesMembraneRelative rolesStressActinRegulationDiverse behaviorsRoleAssemblyTunable Mesoscopic Collagen Island Architectures Modulate Stem Cell Behavior
Nguyen R, Cabral A, Rossello‐Martinez A, Zulli A, Gong X, Zhang Q, Yan J, Mak M. Tunable Mesoscopic Collagen Island Architectures Modulate Stem Cell Behavior. Advanced Materials 2023, 35: e2207882. PMID: 36895051, PMCID: PMC10166061, DOI: 10.1002/adma.202207882.Peer-Reviewed Original ResearchConceptsStem cell behaviorCell behaviorPluripotent stem cellsMesenchymal stem cell behaviorMammalian cellsMesodermal differentiationCell migrationExtracellular matrixStem cellsOsteogenic differentiationRegional enrichmentCollagen-based hydrogelsPhysiological tissuesBioactive cuesDifferentiationBiophysical environmentCollagen hydrogelsCellsIslandsComplex architectureTissue engineering applicationsTissueCollagen architectureEnrichmentGelCorrection: Trio family proteins as regulators of cell migration and morphogenesis in development and disease – mechanisms and cellular contexts
Bircher J, Koleske A. Correction: Trio family proteins as regulators of cell migration and morphogenesis in development and disease – mechanisms and cellular contexts. Journal Of Cell Science 2023, 136 PMID: 36763488, PMCID: PMC11034500, DOI: 10.1242/jcs.260984.Peer-Reviewed Original Research
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