QIF by AQUA

Automated quantitative analysis (AQUA) allows detection of biomarker expression within specific subcellular compartments, as defined by antibody-conjugated fluorophore labelling and co-localization of the target of interest with cytoplasmic or nuclear staining. The fluorescent intensity is measured and divided by the compartment area to yield a quantitative, continuous, and reproducible score for each field of view, and has been validated in tissue microarrays as well as whole tissue sections.