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Antibody Optimization and Titration

Expression range graph for protein X

Antibody titration

Antibody titration is performed to optimize signal to noise ratio and achieve optimal dynamic range for a biomarker of interest. In this example a titer of 1 to 50 is chosen over 1 to 25 because of less background signal.

Western Blot of protein X and QIF of protein X on cell line TMA

Western Blot

After titer optimization the antibody is further validated for reproducibility and specificity (Western Blot)

Reproducibility of protein X