The Specialized Translational Services Lab provides automated quantitation along with in-situ analysis of proteins and nucleic acids.
MissionOur mission is to provide standardized and reproducible high quality analyses of biomarker expression levels. The specialized Translational Services Laboratory (STS Lab) at Yale University is a CLIA-certified research and service facility. We specialize in in-situ quantification of biomarkers in various tissue types, maintaining tissue morphology and spatial information. We offer objective and reproducible quantification of proteins and nucleic acids of formalin fixed paraffin embedded (FFPE) tissues using quantitative immunofluorescence (QIF) to measure biomarkers of interest in tumor/stromal areas and subcellular compartments.
Services Offered in a CLIA-Certified Lab Setting
QIF by AQUA
- Use of AQUA analysis software, which provides automated, quantitative, standardized objective results
Antibody Optimization & Titration
- Antibody Validation
Development of Index Arrays
- Assay development and standardization, including generation of SOPs and reproducibility/control arrays
- Biomarker-specific index array development
- Cohort analysis using TMAs for high throughput discovery work
RNA Biomarker Analysis
- In-situ quantification of mRNA levels
- Quantification of protein expression levels in various tissue types
- Customized Services Available
Director Yale Pathology Tissue Services
Professor of Pathology and of Medicine (Medical Oncology)
Director of Pathology Tissue Services
Director of Translational Pathology
Biomarkers, Pharmacological; Breast Neoplasms; Immunohistochemistry; Medical Oncology; Melanoma; Pathology
David Rimm is a Professor in the Department of Pathology at the Yale University School of Medicine. He completed an MD-PhD at Johns Hopkins University Medical School followed by a Pathology Residency at Yale and a Cytopathology Fellowship at the Medical College of Virginia. He is board certified in Anatomic Pathology and Cytopathology. At Yale since 1994, Dr. Rimm is the Director of Yale Pathology Tissue Services and the Yale Tissue Microarray Facility. He is a member of the Executive Team in...
Director Specialized Translational Services
Associate Research Scientist
I received my Bachelors degree in Biomedical Sciences in 2010 from University College Cork, Ireland and pursued a PhD in molecular biology with a specialized interest in translational research in Triple Negative Breast Cancer. I undertook a postdoctoral fellowship under the mentorship of Dr. Rimm in his academic lab focusing on PD-L1 and it's expression and utility as a biomarker in cancer. In addition I developed a strong interest in the quality control and reproducibility of antibody based...
I graduated from the University of Connecticut in 2004 with a B.S in Pathobiology. I have worked at several biomedical companies since 2004 in the fields of radiation therapy and biomarker imaging. In 2013 I came to Yale to work in the STS lab to pursue my interest in the area of assay development and biomarker imaging .