2015
NFATc1 promotes prostate tumorigenesis and overcomes PTEN loss-induced senescence
Manda K, Tripathi P, Hsi A, Ning J, Ruzinova M, Liapis H, Bailey M, Zhang H, Maher C, Humphrey P, Andriole G, Ding L, You Z, Chen F. NFATc1 promotes prostate tumorigenesis and overcomes PTEN loss-induced senescence. Oncogene 2015, 35: 3282-3292. PMID: 26477312, PMCID: PMC5012433, DOI: 10.1038/onc.2015.389.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCell LineCell Line, TumorCell ProliferationCell Transformation, NeoplasticCellular SenescenceCytokinesGene Expression Regulation, NeoplasticHumansImmunohistochemistryMaleMice, KnockoutMice, NudeMice, TransgenicNFATC Transcription FactorsProstateProstatic NeoplasmsPTEN PhosphohydrolaseReverse Transcriptase Polymerase Chain ReactionTransplantation, HomologousTumor Cells, CulturedTumor MicroenvironmentConceptsProstate tumorigenesisHuman PCaNFATc1 activationNon-tumorigenic prostate cellsActivated T cells c1Cultured PCa cellsT cells c1Cellular senescenceRole of NFATc1Number of cytokinesActivation of NFATc1Proinflammatory cytokinesPCa cellsProstate cancerProstatic adenocarcinomaLuminal epitheliumMouse prostateCells c1Normal prostateOncogenic roleOncogene c-mycProstate tissueProstate cellsSoluble factorsNuclear factor
2009
Loss of Nkx3.1 leads to the activation of discrete downstream target genes during prostate tumorigenesis
Song H, Zhang B, Watson M, Humphrey P, Lim H, Milbrandt J. Loss of Nkx3.1 leads to the activation of discrete downstream target genes during prostate tumorigenesis. Oncogene 2009, 28: 3307-3319. PMID: 19597465, PMCID: PMC2746257, DOI: 10.1038/onc.2009.181.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtrophyClusterinDisease Models, AnimalDisease ProgressionDown-RegulationGene DeletionGene Expression ProfilingGene Expression Regulation, NeoplasticHomeodomain ProteinsHumansLasersMaleMiceMicrodissectionOxidoreductases Acting on Sulfur Group DonorsProstateProstatic NeoplasmsProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-mycPTEN PhosphohydrolaseSignal TransductionThioredoxinsTranscription FactorsTranscription, GeneticTranscriptional ActivationConceptsNKX3.1 lossMolecular consequencesGene expressionProstate tumorigenesisPTEN-AKTCancer initiationProstate cancer initiationCohort of genesNumber of genesC-Myc signaling pathwayDownstream target genesHuman prostate tumorigenesisLoss of NKX3.1NKX3.1 expressionTumor suppressor geneGene expression data setsExpression data setsQuiescin Q6Transcriptional regulatorsIndependent lossesExpression of NKX3.1Laser capture microdissectionTarget genesCancer gene expression data setsSignaling pathways
2001
Impaired prostate tumorigenesis in Egr1-deficient mice
Abdulkadir S, Qu Z, Garabedian E, Song S, Peters T, Svaren J, Carbone J, Naughton C, Catalona W, Ackerman J, Gordon J, Humphrey P, Milbrandt J. Impaired prostate tumorigenesis in Egr1-deficient mice. Nature Medicine 2001, 7: 101-107. PMID: 11135623, DOI: 10.1038/83231.Peer-Reviewed Original ResearchConceptsEarly growth response protein 1Prostate cancerTumor progressionProstatic intra-epithelial neoplasiaEgr1 deficiencyIntra-epithelial neoplasiaHigh-resolution magnetic resonance imagingTransgenic mouse modelHuman prostate cancerTumor growth rateEgr1-deficient miceMagnetic resonance imagingProstate tumor progressionMouse modelProstate tumorigenesisSurvival analysisResonance imagingCarcinomaTumor developmentProtein 1ProgressionCancerMiceDeficiencyEGR1
2000
Evaluation of a chronic indwelling prototype mesh ureteral stent in a porcine model
Olweny E, Portis A, Sundaram C, Afane J, Humphrey P, Ewers R, McDougall E, Clayman R. Evaluation of a chronic indwelling prototype mesh ureteral stent in a porcine model. Urology 2000, 56: 857-862. PMID: 11068323, DOI: 10.1016/s0090-4295(00)00734-2.Peer-Reviewed Original ResearchLAPAROSCOPIC AUGMENTATION CYSTOPLASTY WITH DIFFERENT BIODEGRADABLE GRAFTS IN AN ANIMAL MODEL
PORTIS A, ELBAHNASY A, SHALHAV A, BREWER A, HUMPHREY P, MCDOUGALL E, CLAYMAN R. LAPAROSCOPIC AUGMENTATION CYSTOPLASTY WITH DIFFERENT BIODEGRADABLE GRAFTS IN AN ANIMAL MODEL. Journal Of Urology 2000, 164: 1405-1411. PMID: 10992423, DOI: 10.1016/s0022-5347(05)67207-2.Peer-Reviewed Original ResearchConceptsSmall intestinal submucosaHuman placental membranesAcellular tissue matrixBladder capacityLaparoscopic techniqueSIS groupBladder wall replacementLaparoscopic partial cystectomyTransitional cell epitheliumBovine pericardiumIntracorporeal suturing techniquesEvidence of encrustationSignificant inflammatory responseLapra-Ty clipsHost bladderPartial cystectomyAugmentation cystoplastyBladder augmentationMucosal coverageUrethral catheterBPC groupFree graftEndostitch deviceInflammatory responseBladder wall
1998
A transgenic mouse model of metastatic prostate cancer originating from neuroendocrine cells
Garabedian E, Humphrey P, Gordon J. A transgenic mouse model of metastatic prostate cancer originating from neuroendocrine cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 15382-15387. PMID: 9860977, PMCID: PMC28051, DOI: 10.1073/pnas.95.26.15382.Peer-Reviewed Original ResearchConceptsMetastatic prostate cancerProstatic intraepithelial neoplasiaTransgenic mouse modelProstate cancerIntraepithelial neoplasiaMouse modelNeuroendocrine cellsHuman prostate cancerNeuroendocrine cell lineagesWeeks of ageNeuroendocrine differentiationSimian virus 40 T antigenLocal invasionMouse prostateCancerNeoplasiaProstateWeeksT antigenCell lineagesTransgene expressionMultiple pedigreesCellsMetastasisAndrogens
1997
Comparison of Fibrin Glue, Laser Weld, and Mechanical Suturing Device for the Laparoscopic Closure of Ureterotomy in a Porcine Model
Wolf S, Soble J, Nakada S, Rayala H, Humphrey P, Clayman R, Poppas D. Comparison of Fibrin Glue, Laser Weld, and Mechanical Suturing Device for the Laparoscopic Closure of Ureterotomy in a Porcine Model. Journal Of Urology 1997, 157: 1487-1492. PMID: 9120988, DOI: 10.1016/s0022-5347(01)65029-8.Peer-Reviewed Original ResearchEffect of Canarypox Virus (ALVAC)-Mediated Cytokine Expression on Murine Prostate Tumor Growth
Kawakita M, Rao G, Ritchey J, Ornstein D, Hudson M, Harmon T, Ratliff T, Humphrey P, Tartaglia J, Paoletti E. Effect of Canarypox Virus (ALVAC)-Mediated Cytokine Expression on Murine Prostate Tumor Growth. Journal Of The National Cancer Institute 1997, 89: 428-436. PMID: 9091644, DOI: 10.1093/jnci/89.6.428.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAvipoxvirusB7-1 AntigenCytokinesDisease Models, AnimalFlow CytometryGene Expression Regulation, NeoplasticGene Expression Regulation, ViralGene Transfer TechniquesGenetic VectorsImmunotherapyInterferon-gammaInterleukin-2MaleMiceMice, Inbred C57BLMice, SCIDProstatic NeoplasmsTime FactorsTumor Necrosis Factor-alphaConceptsRM-1 cellsTumor cell expressionMouse prostate cancer cellsIL-2TNF-alphaC57BL/6 miceProstate cancer cellsTumor growthCell expressionTumor sizeB7-1Gene product expressionCo-stimulatory molecules B7-1Canarypox virusCytotoxic T lymphocyte activityKaplan-Meier survival methodMeasurable tumor sizeNonspecific antitumor activitySubsequent tumor challengeT lymphocyte activityCancer cellsCytotoxic T cellsInfected cellsProstate tumor growthMouse prostate tumor modelIn Vivo Comparison of Electrosurgical Vaporization Electrodes
Wolf J, Rayala H, Humphrey P, Clayman R. In Vivo Comparison of Electrosurgical Vaporization Electrodes. Journal Of Endourology 1997, 11: 83-87. PMID: 9048305, DOI: 10.1089/end.1997.11.83.Peer-Reviewed Original ResearchLaparoscopic pyeloplasty in the animal model.
McDougall E, Elashry O, Clayman R, Humphrey P, Rayah H. Laparoscopic pyeloplasty in the animal model. JSLS Journal Of The Society Of Laparoscopic & Robotic Surgeons 1997, 1: 113-8. PMID: 9876657, PMCID: PMC3021264.Peer-Reviewed Original ResearchConceptsLaparoscopic pyeloplastyIntracorporeal suturing techniquesOperative timeEndostitch deviceSecondary ureteropelvic junction obstructionSuturing techniqueDifferent laparoscopic techniquesUnilateral UPJ obstructionLonger operative timeUreteropelvic junction obstructionLapra-Ty clipsRenal functionRenal scanSerum creatinineRenal biopsyUrine cultureGeneral anesthesiaJunction obstructionRetrograde pyelographyUreteral catheterizationLaparoscopic techniqueUPJ obstruction
1996
The Unintubated Ureterotomy Endourologically Revisited
Gardner S, Wolf S, Nakada S, Moon Y, Figenshau R, Pearle M, Soble J, Humphrey P, Clayman R. The Unintubated Ureterotomy Endourologically Revisited. Journal Of Urology 1996, 156: 1160-1163. PMID: 8709338, DOI: 10.1016/s0022-5347(01)65742-2.Peer-Reviewed Original Research
1995
Laparoscopic Pneumodissection: A Unique Means of Tissue Dissection
Gardner S, Clayman R, McDougall E, Moon Y, Fadden P, Anderson K, Pearle M, Royal H, Humphrey P, Pingleton E, Roemer F. Laparoscopic Pneumodissection: A Unique Means of Tissue Dissection. Journal Of Urology 1995, 154: 591-594. PMID: 7609143, DOI: 10.1016/s0022-5347(01)67117-9.Peer-Reviewed Original ResearchConceptsRenal scanRenal hilumBlood gasesChronic tissue effectsRenal damageLaparoscopic proceduresClinical studiesAcute changesAcute damageTissue dissectionLong-term studiesSafe methodTissue effectsBrief burstsHistological sectionsOnly tissueTissuePorcine kidneyHilumDiscernible damageSignificant changesSame tissueScansPorcine tissuesDamageEvaluation of Optimal Stent Size After Endourologic Incision of Ureteral Strictures
Moon Y, Kerbl K, Pearle M, Gardner S, McDougall E, Humphrey P, Clayman R. Evaluation of Optimal Stent Size After Endourologic Incision of Ureteral Strictures. Journal Of Endourology 1995, 9: 15-22. PMID: 7780426, DOI: 10.1089/end.1995.9.15.Peer-Reviewed Original Research
1994
Comparison of Retrograde Endo-Pyelotomy and Endo-Balloon Rupture of the Ureteropelvic Junction in a Porcine Model
Pearle M, Moon Y, Endicott R, Gardner S, Humphrey P, Clayman R. Comparison of Retrograde Endo-Pyelotomy and Endo-Balloon Rupture of the Ureteropelvic Junction in a Porcine Model. Journal Of Urology 1994, 152: 2232-2239. PMID: 7966717, DOI: 10.1016/s0022-5347(17)31649-x.Peer-Reviewed Original ResearchConceptsUreteropelvic junctionRetroperitoneal extravasationRetrograde pyelogramStent removalUreteral stentsAdventitial layerSecondary ureteropelvic junction obstructionPatent ureteropelvic junctionDouble pigtail ureteral stentUreteropelvic junction obstructionFemale farm pigsChronic study animalsPigtail ureteral stentChronic animal studiesNormal ureteropelvic junctionBalloon distentionBalloon incisionUrine extravasationAcute groupLinear tearChronic groupChronic inflammationMild fibrosisJunction obstructionCommon findingDevelopment of a Mouse Model for Nonbacterial Prostatitis
Keetch D, Humphrey P, Ratliff T. Development of a Mouse Model for Nonbacterial Prostatitis. Journal Of Urology 1994, 152: 247-250. PMID: 8201676, DOI: 10.1016/s0022-5347(17)32871-9.Peer-Reviewed Original ResearchConceptsNonbacterial prostatitisProstatic inflammationC57BL/6-lpr miceLpr miceBALB/c miceAdoptive transfer studiesCommon clinical entityDegree of inflammationBALB/cPeriglandular regionsAutoimmune processAJ miceLymphocytic infiltrationC57BL/6 miceClinical entityProstate antigenC miceImmune parametersSyngeneic miceProstatitisMouse modelProstatic tissueDisease processAnimal modelsInflammation
1993
Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas
Wikstrand C, Stanley S, Humphrey P, Pegram C, Archer G, Kurpad S, Shibuya M, Bigner D. Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas. Journal Of Neuroimmunology 1993, 46: 165-173. PMID: 8360327, DOI: 10.1016/0165-5728(93)90246-u.Peer-Reviewed Original ResearchConceptsEpidermal growth factor receptorGrowth factor receptorVariant receptorHuman epidermal growth factor receptorFactor receptorVariant epidermal growth factor receptorResponse of miceHuman tumor samplesAdditional immunizationsPeptide immunizationAntibody activityAntibody productionSynthetic peptidesTumor samplesImmunizationReceptorsRabbitsSingle goatGliomasTitersReceptor proteinVariant formImmunogenPeptides
1988
Comparative localization of murine monoclonal antibody Me1-14 F(ab')2 fragment and whole IgG2a in human glioma xenografts.
Colapinto E, Humphrey P, Zalutsky M, Groothuis D, Friedman H, de Tribolet N, Carrel S, Bigner D. Comparative localization of murine monoclonal antibody Me1-14 F(ab')2 fragment and whole IgG2a in human glioma xenografts. Cancer Research 1988, 48: 5701-7. PMID: 3167830.Peer-Reviewed Original ResearchConceptsHuman glioma cell line DNormal tissuesSpecific tumor localizationHuman glioma xenograftsXenograft-bearing miceNormal tissue ratiosHigh radiation dosesImmunoglobulin G2aAthymic miceGlioma xenograftsBrain tumorsBrain tumor diagnosisTumor localizationHigh tumorTumor uptakeIntracranial xenograftsComparative localizationTherapeutic agentsMonoclonal antibodiesMAb fragmentsTissue ratioTumor diagnosisPotential agentRadiation dosesSelective deliveryThe Localisation of Radiolabeled Murine Monoclonal Antibody 81C6 and its Fab Fragment in Human Glioma Xenografts in Athymic Mice
Colapinto E, Lee Y, Humphrey P, Zalutsky M, Friedman H, Bullard D, Bigner D. The Localisation of Radiolabeled Murine Monoclonal Antibody 81C6 and its Fab Fragment in Human Glioma Xenografts in Athymic Mice. British Journal Of Neurosurgery 1988, 2: 179-191. PMID: 3267302, DOI: 10.3109/02688698808992668.Peer-Reviewed Original ResearchConceptsMab 81C6Monoclonal antibody 81C6Dose/Athymic miceFab fragmentsHuman glioma xenograftsMG glioma cellsGlioma xenograftsHigh immunoreactivityIntracranial xenograftsGlioma cellsExtracellular matrix antigensNormal tissuesTissue ratioMarked lossRadiation dosesXenograftsImmunoreactivityTumorsMatrix antigensAntigenMiceTheoretical benefitsFab levelsGliomas