2020
Acetaminophen Attenuates invasion and alters the expression of extracellular matrix enzymes and vascular factors in human first trimester trophoblast cells
Burman A, Garcia-Milian R, Wood M, DeWitt NA, Vasiliou V, Guller S, Abrahams VM, Whirledge S. Acetaminophen Attenuates invasion and alters the expression of extracellular matrix enzymes and vascular factors in human first trimester trophoblast cells. Placenta 2020, 104: 146-160. PMID: 33348283, DOI: 10.1016/j.placenta.2020.12.002.Peer-Reviewed Original ResearchConceptsFirst trimester trophoblast cellsHuman first trimester trophoblast cellsPrenatal acetaminophen useTrophoblast cellsAcetaminophen useTrophoblast functionsAcetaminophen exposureFetal developmentHTR-8/SVneo cellsPrenatal acetaminophen exposureAnti-inflammatory drug aspirinRecent epidemiological studiesPlacental cell typesMatrix metalloproteinases 2Expression of angiogenesisExtracellular matrix enzymesPrenatal acetaminophenCommon medicationsVascular factorsMaternal healthPlacental functionEpidemiological associationLong-term effectsCyclooxygenase-1Epidemiological studiesTrophoblast-secreted soluble-PD-L1 modulates macrophage polarization and function
Zhang Y, Aldo P, You Y, Ding J, Kaislasuo J, Petersen JF, Lokkegaard E, Peng G, Paidas MJ, Simpson S, Pal L, Guller S, Liu H, Liao AH, Mor G. Trophoblast-secreted soluble-PD-L1 modulates macrophage polarization and function. Journal Of Leukocyte Biology 2020, 108: 983-998. PMID: 32386458, PMCID: PMC8190653, DOI: 10.1002/jlb.1a0420-012rr.Peer-Reviewed Original ResearchConceptsSoluble PD-L1Macrophage polarizationTrophoblast cellsCell death ligand 1PD-L1/PDPD-1 blockageDeath ligand 1Macrophage differentiationModulates macrophage polarizationExpression/secretionDecidual macrophagesHealthy pregnancyPD-L1Pregnant womenTLR4 ligationM2 phenotypeExpression of typeFetal developmentLPS activationMacrophagesIFNPlacenta developmentLigand 1Constitutive productionGestation
2014
Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages
Aldo PB, Racicot K, Craviero V, Guller S, Romero R, Mor G. Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages. American Journal Of Reproductive Immunology 2014, 72: 270-284. PMID: 24995492, PMCID: PMC4230492, DOI: 10.1111/aji.12288.Peer-Reviewed Original ResearchConceptsUnique macrophage phenotypeMacrophage phenotypeMonocyte differentiationFirst trimester trophoblast cellsPeripheral blood monocytesPhenotype of macrophagesCD14 surface expressionNormal deciduaHealthy pregnancyIL-10Chemokine productionEarly pregnancyIP-10Monocyte exposureIL-1BBlood monocytesDecidual phenotypePhagocytic capacityTrophoblast cellsFlow cytometryMorphologic changesMacrophagesMonocytesSurface expressionPlacenta development
2010
Role of GnRH–GnRH receptor signaling at the maternal-fetal interface
Lee HJ, Snegovskikh VV, Park JS, Foyouzi N, Han KT, Hodgson EJ, Guller S, Norwitz ER. Role of GnRH–GnRH receptor signaling at the maternal-fetal interface. Fertility And Sterility 2010, 94: 2680-2687. PMID: 20400076, DOI: 10.1016/j.fertnstert.2010.03.016.Peer-Reviewed Original ResearchConceptsGnRHR-I mRNAMaternal-fetal interfaceDecidual stromal cellsHCG productionGnRH-IISelect cytokinesTrophoblast cellsCell linesEffects of GnRHUniversity Teaching HospitalExpression of GnRHFunction of GnRHReverse transcription-polymerase chain reactionDose-dependent fashionHuman trophoblast cellsTerm deliveryPolymerase chain reactionTeaching hospitalGnRH receptorMAIN OUTCOMEGnRHHuman trophoblastsStromal cellsWestern blotImmortalized cell lines
2009
The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71
Straszewski-Chavez SL, Abrahams VM, Alvero AB, Aldo PB, Ma Y, Guller S, Romero R, Mor G. The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71. Placenta 2009, 30: 939-948. PMID: 19766308, PMCID: PMC2784169, DOI: 10.1016/j.placenta.2009.08.007.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDAntigens, Differentiation, MyelomonocyticApoptosisCell LineChorionic GonadotropinCytokinesFemaleFibronectinsHistocompatibility Antigens Class IHLA AntigensHLA-G AntigensHumansIntercellular Signaling Peptides and ProteinsKeratin-7Leukocyte Common AntigensPregnancyPregnancy Trimester, FirstTelomeraseThy-1 AntigensTrophoblastsVimentinConceptsFirst trimester trophoblast cell lineSwan 71 cellsTrophoblast cell lineFirst trimester trophoblast cellsFibroblast-specific antigenHuman chorionic gonadotrophinPrimary trophoblast cellsTrophoblast cellsHuman telomerase reverse transcriptaseFetal fibronectinCell linesCD90/ThySwan 71Growth factor profileTNF-alpha-induced apoptosisChorionic gonadotrophinCytokeratin 7Specific antigenTelomerase reverse transcriptaseNormal placentaFactor profileKaryotypic abnormalitiesPatient samplesPrimary human cellsAdequate cell numbersORIGINAL ARTICLE: Regulation of Nod1 and Nod2 in First Trimester Trophoblast Cells
Mulla MJ, Yu AG, Cardenas I, Guller S, Panda B, Abrahams VM. ORIGINAL ARTICLE: Regulation of Nod1 and Nod2 in First Trimester Trophoblast Cells. American Journal Of Reproductive Immunology 2009, 61: 294-302. PMID: 19260860, DOI: 10.1111/j.1600-0897.2009.00694.x.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisBenzamidesCells, CulturedCytokinesDiaminopimelic AcidFemaleGene Expression Regulation, DevelopmentalHumansLipopolysaccharidesNF-kappa BNod1 Signaling Adaptor ProteinNod2 Signaling Adaptor ProteinPregnancyPregnancy Trimester, FirstSequence DeletionSignal TransductionThiazolesToll-Like Receptor 4TransgenesTrophoblastsConceptsFirst trimester trophoblast cellsTrophoblast cellsExpression of NOD1Cytoplasmic pattern recognition receptorsProduction of cytokinesNOD2 mRNA expressionPattern recognition receptorsNOD2 expressionCytokine responsesInflammatory responseNOD2 activationNOD1 activationNFkappaB inhibitorRecognition receptorsNOD1Nod1 stimulationNOD2Bacterial lipopolysaccharideMRNA expressionTLR4LipopolysaccharideNFkappaB pathwayRT-PCRBacterial peptidesTrophoblast
2005
A Role for TLRs in the Regulation of Immune Cell Migration by First Trimester Trophoblast Cells
Abrahams VM, Visintin I, Aldo PB, Guller S, Romero R, Mor G. A Role for TLRs in the Regulation of Immune Cell Migration by First Trimester Trophoblast Cells. The Journal Of Immunology 2005, 175: 8096-8104. PMID: 16339547, DOI: 10.4049/jimmunol.175.12.8096.Peer-Reviewed Original ResearchConceptsFirst trimester trophoblast cellsInnate immune cellsMaternal-fetal interfaceTrophoblast cellsIntrauterine infectionPregnancy complicationsNormal pregnancyImmune cellsImmune responseImmune systemHuman first trimester trophoblast cellsGrowth-related oncogene alphaCertain pregnancy complicationsElevated leukocyte infiltrationMaternal immune systemGrowth-related oncogeneTLR-3 stimulationImmune cell migrationSecretion of chemokinesInnate immune systemElevated monocytesPaternal AgsPreterm laborNK cellsTLR-3
2004
First trimester trophoblast cells secrete Fas ligand which induces immune cell apoptosis
Abrahams VM, Straszewski-Chavez S, Guller S, Mor G. First trimester trophoblast cells secrete Fas ligand which induces immune cell apoptosis. Molecular Human Reproduction 2004, 10: 55-63. PMID: 14665707, DOI: 10.1093/molehr/gah006.Peer-Reviewed Original ResearchConceptsFirst trimester trophoblast cellsImmune privilegeTrophoblast cellsFas/Fas ligand (FasL) apoptotic pathwayMaternal immune recognitionMaternal immune systemImmune cell apoptosisSecretion of FasLT cell deathPaternal alloantigensAllograft rejectionNormal pregnancyPeripheral toleranceImmune recognitionImmune systemRelease of microvesiclesFas ligandCell apoptosisVivo studiesFasLMembrane-associated expressionTrophoblastIntracellular FasLApoptotic pathwayDeath