2016
Direct vitamin D3 actions on rhesus macaque follicles in three-dimensional culture: assessment of follicle survival, growth, steroid, and antimüllerian hormone production
Xu J, Hennebold JD, Seifer DB. Direct vitamin D3 actions on rhesus macaque follicles in three-dimensional culture: assessment of follicle survival, growth, steroid, and antimüllerian hormone production. Fertility And Sterility 2016, 106: 1815-1820.e1. PMID: 27678030, PMCID: PMC5136302, DOI: 10.1016/j.fertnstert.2016.08.037.Peer-Reviewed Original ResearchConceptsVitamin D3Follicle survivalPreantral follicle survivalAntral folliclesWeek 5High-dose vitamin D3Direct actionAntimüllerian hormone concentrationsAntral follicle diameterPrimate follicular developmentVitamin D3 actionAntral follicle growthDihydroxy vitamin D3Rhesus monkey ovariesSecondary preantral folliclesFemale rhesus macaquesMacaque folliclesPrimate follicleD3 actionAMH concentrationsNational Primate Research CenterAMH productionPrimate Research CenterFollicle diameterMAIN OUTCOME
2000
Progesterone, Inhibin, and hCG Multiple Marker Strategy to Differentiate Viable From Nonviable Pregnancies
PHIPPS M, HOGAN J, PEIPERT J, LAMBERT-MESSERLIAN G, CANICK J, SEIFER D. Progesterone, Inhibin, and hCG Multiple Marker Strategy to Differentiate Viable From Nonviable Pregnancies. Obstetrics And Gynecology 2000, 95: 227-231. DOI: 10.1097/00006250-200002000-00011.Peer-Reviewed Original ResearchMeSH KeywordsAdultBiomarkersChorionic GonadotropinChorionic Gonadotropin, beta Subunit, HumanCohort StudiesFemaleFetal ViabilityHumansInhibinsPredictive Value of TestsPregnancyPregnancy ComplicationsPregnancy OutcomePregnancy Trimester, FirstProgesteroneProspective StudiesROC CurveSensitivity and SpecificityConceptsDual-biomarker strategyMultiple biomarker strategyNonviable pregnanciesSerum progesteroneBiomarker strategiesComplaints of painProspective cohort studySymptomatic pregnant womenCombination of progesteroneCohort studyCombination of serumInhibin AUrine biomarkersFirst trimesterPregnant womenEarly gestationNonviable outcomesPregnancySingle biomarkerHCGProgesteroneSpecific biomarkersDiagnostic accuracySerum samplesBiomarkersProgesterone, inhibin, and hCG multiple marker strategy to differentiate viable from nonviable pregnancies.
Phipps M, Hogan J, Peipert J, Lambert-Messerlian G, Canick J, Seifer D. Progesterone, inhibin, and hCG multiple marker strategy to differentiate viable from nonviable pregnancies. Obstetrics And Gynecology 2000, 95: 227-31. PMID: 10674584, DOI: 10.1016/s0029-7844(99)00480-9.Peer-Reviewed Original ResearchMeSH KeywordsAdultBiomarkersChorionic GonadotropinChorionic Gonadotropin, beta Subunit, HumanCohort StudiesFemaleFetal ViabilityHumansInhibinsPredictive Value of TestsPregnancyPregnancy ComplicationsPregnancy OutcomePregnancy Trimester, FirstProgesteroneProspective StudiesROC CurveSensitivity and SpecificityConceptsDual-biomarker strategyMultiple biomarker strategyNonviable pregnanciesSerum progesteroneBiomarker strategiesComplaints of painProspective cohort studySymptomatic pregnant womenCombination of progesteroneCohort studyCombination of serumInhibin AUrine biomarkersFirst trimesterPregnant womenEarly gestationNonviable outcomesPregnancySingle biomarkerHCGProgesteroneSpecific biomarkersDiagnostic accuracySerum samplesBiomarkers
1999
Diagnosis of acute and persistent ectopic pregnancy.
Graczykowski JW, Seifer DB. Diagnosis of acute and persistent ectopic pregnancy. Clinical Obstetrics & Gynecology 1999, 42: 9-22; quiz 55-6. PMID: 10073295, DOI: 10.1097/00003081-199903000-00005.Peer-Reviewed Original Research
1998
Activin a stimulates meiotic maturation of human oocytes and modulates granulosa cell steroidogenesis in vitro
Alak B, Coskun S, Friedman C, Kennard E, Kim M, Seifer D. Activin a stimulates meiotic maturation of human oocytes and modulates granulosa cell steroidogenesis in vitro. Fertility And Sterility 1998, 70: 1126-1130. PMID: 9848305, DOI: 10.1016/s0015-0282(98)00386-0.Peer-Reviewed Original ResearchConceptsGranulosa cell steroidogenesisGerminal vesicle breakdownGranulosa cellsActivin AE2 secretionSteroid secretionCell steroidogenesisNonluteinized granulosa cellsMeiotic maturationMetaphase IIUniversity Medical CenterAntral granulosa cellsNonovarian pathologyInhibin AMedical CenterMAIN OUTCOMEActivin A.Oocyte meiotic maturationImmature oocytesHuman oocytesOocyte maturationSecretionVesicle breakdownProgesteroneSteroidogenesisDimeric inhibin: a direct marker of ovarian aging
Danforth D, Arbogast L, Mroueh J, Kim M, Kennard E, Seifer D, Friedman C. Dimeric inhibin: a direct marker of ovarian aging. Fertility And Sterility 1998, 70: 119-123. PMID: 9660432, DOI: 10.1016/s0015-0282(98)00127-7.Peer-Reviewed Original ResearchConceptsInhibin B levelsPerimenopausal transitionOvarian reserveRegular menstrual cyclesPituitary FSH secretionDaily urine samplesSubsequent follicular phaseCorpus luteum functionYears of ageDay of ovulationWomen 39FSH levelsInhibin secretionFSH secretionPerimenopausal womenSerum levelsLH surgeProspective studyOvarian functionFollicular phaseMenstrual cycleFifth decadeMAIN OUTCOMEBlood samplesLuteal secretion
1996
Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis**Presented in part at the 51st Annual Meeting of the American Society for Reproductive Medicine. Seattle, Washington, October 7 to 12, 1995.††Supported in part by Physician Scientist Award (AG00566 [D.B.S.] and R01HD31894 [A.L.S.]) from the National Institutes of Health, National Institute on Aging, Bethesda, Maryland.
Seifer D, Freeman M, Gardiner A, Hill G, Schneyer A, Vanderhyden B. Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis**Presented in part at the 51st Annual Meeting of the American Society for Reproductive Medicine. Seattle, Washington, October 7 to 12, 1995.††Supported in part by Physician Scientist Award (AG00566 [D.B.S.] and R01HD31894 [A.L.S.]) from the National Institutes of Health, National Institute on Aging, Bethesda, Maryland. Fertility And Sterility 1996, 66: 425-429. PMID: 8751742, DOI: 10.1016/s0015-0282(16)58513-6.Peer-Reviewed Original ResearchConceptsLuteinized granulosa cellsGranulosa cellsStandard IVF-ET treatment cycleIVF-ET treatment cyclesTwo-pronuclear embryosGranulosa cell steroidogenesisTwo-pronuclear zygotesNational InstituteVitro Fertilization UnitProduction of E2Steroid hormone productionPituitary desensitizationOvarian stimulationFollicular aspiratesCell steroidogenesisFertilization unitTreatment cyclesHormone productionCell coculturesReproductive medicineP productionAmerican SocietyAcademic research environmentE2Human embryosSerum inhibin levels are lower in ectopic than intrauterine spontaneously conceived pregnancies*†*Presented in part at the 42nd Annual Meeting of the Society for Gynecological Investigation, Chicago, Illinois, March 15 to 18, 1995.†Supported in part by Physician Scientist Award from National Institutes of Health, National Institute on Aging grant AG00566 (D.B.S.) and HD29164 (G.M.L.M., A.L.S.).
Seifer D, Lambert-Messerlian G, Canick J, Frishman G, Schneyer A. Serum inhibin levels are lower in ectopic than intrauterine spontaneously conceived pregnancies*†*Presented in part at the 42nd Annual Meeting of the Society for Gynecological Investigation, Chicago, Illinois, March 15 to 18, 1995.†Supported in part by Physician Scientist Award from National Institutes of Health, National Institute on Aging grant AG00566 (D.B.S.) and HD29164 (G.M.L.M., A.L.S.). Fertility And Sterility 1996, 65: 667-669. PMID: 8774307, DOI: 10.1016/s0015-0282(16)58174-6.Peer-Reviewed Original ResearchConceptsIntrauterine pregnancySerum inhibin concentrationsDimeric inhibinInhibin concentrationsTotal inhibinMaternal serumSerum samplesDimeric inhibin concentrationSingle intrauterine pregnancySerum inhibin levelsCase-control studyAcademic clinical practiceNational InstituteAlpha-inhibin subunitGynecological InvestigationInhibin levelsSonographic evidenceGestational ageInhibin subunitsWeek 8MAIN OUTCOMEClinical practiceInhibinEctopicWomenDifferential secretion of dimeric inhibin in cultured luteinized granulosa cells as a function of ovarian reserve
Seifer DB, Gardiner AC, Lambert-Messerlian G, Schneyer AL. Differential secretion of dimeric inhibin in cultured luteinized granulosa cells as a function of ovarian reserve. The Journal Of Clinical Endocrinology & Metabolism 1996, 81: 736-739. PMID: 8636296, DOI: 10.1210/jcem.81.2.8636296.Peer-Reviewed Original ResearchConceptsDay 3 serum FSHLuteinized granulosa cellsHigh FSH groupDimeric inhibinSerum FSHFSH groupPg/Granulosa cellsFSH levelsOvarian reserveOvulation inductionProgesterone concentrationsDay 3 serum FSH levelsCultured luteinized granulosa cellsDimeric inhibin productionLow FSH groupSerum FSH levelsFertilization-embryo transferHigher FSH levelsReproductive technology programGranulosa cell levelTotal inhibinInhibin concentrationsInhibin productionProspective studyRelaxin secretion in in vitro fertilization pregnancies
Haning R, Goldsmith L, Seifer D, Wheeler C, Frishman G, Sarmento J, Weiss G. Relaxin secretion in in vitro fertilization pregnancies. American Journal Of Obstetrics And Gynecology 1996, 174: 233-240. PMID: 8572013, DOI: 10.1016/s0002-9378(96)70400-3.Peer-Reviewed Original ResearchConceptsHuman chorionic gonadotropinSecretion of relaxinChorionic gonadotropinCorpora luteaSerum estradiolSerum relaxinFunctional statusHuman chorionic gonadotropin (hCG) injectionOvarian hyperstimulation syndromeSerum of womenSecretion of steroidsSerum relaxin concentrationsFertilization pregnanciesHyperstimulation syndromePremature deliveryMultiple gestationsGonadotropin injectionRelaxin secretionMore injectionsRelaxin concentrationsProgesterone concentrationsMultiple linear regression analysisPregnancyGonadotropinSteroid concentrations
1994
Early and late presentation of the ovarian hyperstimulation syndrome: two distinct entities with different risk factors*
Lyons CA, Wheeler CA, Frishman GN, Hackett RJ, Seifer DB, Haning RV. Early and late presentation of the ovarian hyperstimulation syndrome: two distinct entities with different risk factors*. Human Reproduction 1994, 9: 792-799. PMID: 7929724, DOI: 10.1093/oxfordjournals.humrep.a138598.Peer-Reviewed Original ResearchConceptsLate ovarian hyperstimulation syndromeOvarian hyperstimulation syndromeSevere ovarian hyperstimulation syndromeNumber of oocytesHyperstimulation syndromeEarly ovarian hyperstimulation syndromePost-human chorionic gonadotrophinDifferent risk factorsStepwise logistic regressionSeries of casesDay-hCGMultiple gestationsClinical predictorsFertilization cyclesGestational sacEarly pregnancyLate presentationOestradiol concentrationsSerum concentrationsEgg retrievalRisk factorsHigh oestradiolAcute effectsChorionic gonadotrophinRetrospective analysis
1992
Luteinizing response to human chorionic gonadotropin does not predict outcome in gonadotropin releasing hormone agonist-suppressed/human menopausal gonadotropin-stimulated in vitro fertilization (IVF) cycles
Penzias A, Shamma F, Gutmann J, Seifer D, DeCherney A, Lavy G. Luteinizing response to human chorionic gonadotropin does not predict outcome in gonadotropin releasing hormone agonist-suppressed/human menopausal gonadotropin-stimulated in vitro fertilization (IVF) cycles. Journal Of Assisted Reproduction And Genetics 1992, 9: 244-247. PMID: 1525454, DOI: 10.1007/bf01203821.Peer-Reviewed Original Research