1996
Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis**Presented in part at the 51st Annual Meeting of the American Society for Reproductive Medicine. Seattle, Washington, October 7 to 12, 1995.††Supported in part by Physician Scientist Award (AG00566 [D.B.S.] and R01HD31894 [A.L.S.]) from the National Institutes of Health, National Institute on Aging, Bethesda, Maryland.
Seifer D, Freeman M, Gardiner A, Hill G, Schneyer A, Vanderhyden B. Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis**Presented in part at the 51st Annual Meeting of the American Society for Reproductive Medicine. Seattle, Washington, October 7 to 12, 1995.††Supported in part by Physician Scientist Award (AG00566 [D.B.S.] and R01HD31894 [A.L.S.]) from the National Institutes of Health, National Institute on Aging, Bethesda, Maryland. Fertility And Sterility 1996, 66: 425-429. PMID: 8751742, DOI: 10.1016/s0015-0282(16)58513-6.Peer-Reviewed Original ResearchConceptsLuteinized granulosa cellsGranulosa cellsStandard IVF-ET treatment cycleIVF-ET treatment cyclesTwo-pronuclear embryosGranulosa cell steroidogenesisTwo-pronuclear zygotesNational InstituteVitro Fertilization UnitProduction of E2Steroid hormone productionPituitary desensitizationOvarian stimulationFollicular aspiratesCell steroidogenesisFertilization unitTreatment cyclesHormone productionCell coculturesReproductive medicineP productionAmerican SocietyAcademic research environmentE2Human embryos
1993
Absence of Immunoreactive Cytokines in Supernatants of Individual Preimplantation Human Embryos
SEIFER D, ROMERO R, BERLINSKY D, HANING R. Absence of Immunoreactive Cytokines in Supernatants of Individual Preimplantation Human Embryos. American Journal Of Reproductive Immunology 1993, 30: 105-107. PMID: 8311918, DOI: 10.1111/j.1600-0897.1993.tb00610.x.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayPreimplantation human embryosSpecific cytokine productionIL-1 betaAvailable enzyme-linked immunosorbent assayIL-1 alphaImmunoreactive cytokinesCytokine productionIndividual preimplantation embryosIL-6Human embryosCommercial assaysImmunosorbent assayCytokinesAvailable kitsPreimplantation embryosSupernatantAssays