Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization*†
Seifer D, Gardiner A, Ferreira K, Peluso J. Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization*†. Fertility And Sterility 1996, 66: 593-598. PMID: 8816622, DOI: 10.1016/s0015-0282(16)58573-2.Peer-Reviewed Original ResearchConceptsDay 3 serum FSHFSH levelsOvarian reserveSerum FSHMIU/Granulosa cellsCell countDiminished ovarian reserveMIU/mLAcademic clinical practiceLuteinized granulosa cellsApoptotic granulosa cellsIVF-ETFollicular aspiratesPreovulatory folliclesClinical practiceFlow cytometryWomenMean numberApoptotic cellsFSHGreater percentageFolliclesCellsViable cellsAutologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis**Presented in part at the 51st Annual Meeting of the American Society for Reproductive Medicine. Seattle, Washington, October 7 to 12, 1995.††Supported in part by Physician Scientist Award (AG00566 [D.B.S.] and R01HD31894 [A.L.S.]) from the National Institutes of Health, National Institute on Aging, Bethesda, Maryland.
Seifer D, Freeman M, Gardiner A, Hill G, Schneyer A, Vanderhyden B. Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis**Presented in part at the 51st Annual Meeting of the American Society for Reproductive Medicine. Seattle, Washington, October 7 to 12, 1995.††Supported in part by Physician Scientist Award (AG00566 [D.B.S.] and R01HD31894 [A.L.S.]) from the National Institutes of Health, National Institute on Aging, Bethesda, Maryland. Fertility And Sterility 1996, 66: 425-429. PMID: 8751742, DOI: 10.1016/s0015-0282(16)58513-6.Peer-Reviewed Original ResearchConceptsLuteinized granulosa cellsGranulosa cellsStandard IVF-ET treatment cycleIVF-ET treatment cyclesTwo-pronuclear embryosGranulosa cell steroidogenesisTwo-pronuclear zygotesNational InstituteVitro Fertilization UnitProduction of E2Steroid hormone productionPituitary desensitizationOvarian stimulationFollicular aspiratesCell steroidogenesisFertilization unitTreatment cyclesHormone productionCell coculturesReproductive medicineP productionAmerican SocietyAcademic research environmentE2Human embryos