2014
Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation
Yang-Hartwich Y, Romanoff E, Bingham J, Alvero AB, Mor G. Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation. Methods In Molecular Biology 2014, 1219: 87-93. PMID: 25308264, DOI: 10.1007/978-1-4939-1661-0_8.Peer-Reviewed Original ResearchConceptsTranscriptional activityP53 transcriptional activityChromatin immunoprecipitationTranscription-dependent activityDNA-binding domainExpression of hundredsImportant cellular functionsKey transcriptional mediatorAbility of p53Protein's transcriptional activityP53-induced apoptosisResult of mutationsGenome integrityCellular homeostasisCellular functionsTranscriptional mediatorsTarget genesPromoter regionApoptotic genesCell deathReal-time quantitative polymerase chain reactionCancer cellsQuantitative polymerase chain reactionImmunoprecipitationGenes
2013
Phenotypic modifications in ovarian cancer stem cells following Paclitaxel treatment
Craveiro V, Yang-Hartwich Y, Holmberg JC, Joo WD, Sumi NJ, Pizzonia J, Griffin B, Gill SK, Silasi DA, Azodi M, Rutherford T, Alvero AB, Mor G. Phenotypic modifications in ovarian cancer stem cells following Paclitaxel treatment. Cancer Medicine 2013, 2: 751-762. PMID: 24403249, PMCID: PMC3892380, DOI: 10.1002/cam4.115.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic Agents, PhytogenicCarcinoma, Ovarian EpithelialDrug Resistance, NeoplasmFemaleHEK293 CellsHumansHyaluronan ReceptorsMiceMice, NudeMyeloid Differentiation Factor 88Neoplasms, Glandular and EpithelialNeoplastic Stem CellsOvarian NeoplasmsPaclitaxelPhenotypeRecurrenceSnail Family Transcription FactorsTranscription FactorsTumor BurdenXenograft Model Antitumor AssaysConceptsEpithelial ovarian cancerRecurrent epithelial ovarian cancerOvarian cancer stem cellsEOC stem cellsCancer stem cellsQuantitative polymerase chain reactionRecurrent diseaseOvarian cancerEOC cellsVivo ovarian cancer modelsStem cellsDoses of paclitaxelLethal gynecologic malignancyOvarian cancer modelProcess of recurrenceWestern blot analysisMaintenance therapyGynecologic malignanciesPrimary diseaseAggressive diseaseEOC patientsPrimary tumorPolymerase chain reactionAggressive phenotypePaclitaxel treatment