2020
NLRP3 inflammasome function and pyroptotic cell death in human placental Hofbauer cells
Abrahams VM, Tang Z, Mor G, Guller S. NLRP3 inflammasome function and pyroptotic cell death in human placental Hofbauer cells. Journal Of Reproductive Immunology 2020, 142: 103214. PMID: 33152658, PMCID: PMC7770077, DOI: 10.1016/j.jri.2020.103214.Peer-Reviewed Original ResearchConceptsHofbauer cellsCaspase-1 activationIL-1β processingIL-1βInflammasome activationFetal inflammatory response syndromeSubsequent pregnancy complicationsInflammatory response syndromeCell deathNLRP3 inflammasome activationInterleukin-1 betaIL-1β secretionInhibition of P2X7Human term placentaGasdermin D cleavageNLRP3 knockdownPlacental inflammationPyroptotic cell deathFetal inflammationHistologic chorioamnionitisPregnancy complicationsResponse syndromeNLRP3 inflammasomeProtein/gene expressionInflammatory formTrophoblast-secreted soluble-PD-L1 modulates macrophage polarization and function
Zhang Y, Aldo P, You Y, Ding J, Kaislasuo J, Petersen JF, Lokkegaard E, Peng G, Paidas MJ, Simpson S, Pal L, Guller S, Liu H, Liao AH, Mor G. Trophoblast-secreted soluble-PD-L1 modulates macrophage polarization and function. Journal Of Leukocyte Biology 2020, 108: 983-998. PMID: 32386458, PMCID: PMC8190653, DOI: 10.1002/jlb.1a0420-012rr.Peer-Reviewed Original ResearchConceptsSoluble PD-L1Macrophage polarizationTrophoblast cellsCell death ligand 1PD-L1/PDPD-1 blockageDeath ligand 1Macrophage differentiationModulates macrophage polarizationExpression/secretionDecidual macrophagesHealthy pregnancyPD-L1Pregnant womenTLR4 ligationM2 phenotypeExpression of typeFetal developmentLPS activationMacrophagesIFNPlacenta developmentLigand 1Constitutive productionGestationHerpesvirus-infected Hofbauer cells activate endothelial cells through an IL-1β-dependent mechanism
Hendrix P, Tang Z, Silasi M, Racicot KE, Mor G, Abrahams VM, Guller S. Herpesvirus-infected Hofbauer cells activate endothelial cells through an IL-1β-dependent mechanism. Placenta 2020, 91: 59-65. PMID: 32174308, PMCID: PMC7078070, DOI: 10.1016/j.placenta.2020.01.010.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsHofbauer cellsIL-1βAdhesion molecule-1Placental viral infectionsIL-8Viral infectionFetal inflammationEndothelial cellsE-selectinMolecule-1IL-1 receptor antagonistVascular adhesion molecule-1Intercellular adhesion molecule-1Adverse pregnancy outcomesMHV-68 infectionIL-1β secretionICAM-1 mRNAUmbilical vein endothelial cellsUmbilical endothelial cellsPregnancy outcomesVein endothelial cellsIL-1raPlacental macrophagesHUVEC expression
2016
Zika virus infection of Hofbauer cells
Simoni MK, Jurado KA, Abrahams VM, Fikrig E, Guller S. Zika virus infection of Hofbauer cells. American Journal Of Reproductive Immunology 2016, 77 PMID: 27966815, PMCID: PMC5299062, DOI: 10.1111/aji.12613.Peer-Reviewed Original ResearchConceptsCongenital Zika syndromeHofbauer cellsZika virusZIKV infectionDevelopment of CZSDengue virusSpread of ZIKVVertical transmissionFetal placental macrophagesPlacental Hofbauer cellsZika virus infectionAntenatal infectionNeonatal outcomesPlacental responsesZika syndromeVirus infectionCurrent evidenceCongenital abnormalitiesRecent studiesNeonatal developmentFetal capillariesRelated flavivirusesInfectionSpecific molecular mechanismsCertain viruses
2014
Toll‐like Receptor‐Mediated Responses by Placental Hofbauer Cells (HBCs): A Potential Pro‐Inflammatory Role for Fetal M2 Macrophages
Young OM, Tang Z, Niven‐Fairchild T, Tadesse S, Krikun G, Norwitz ER, Mor G, Abrahams VM, Guller S. Toll‐like Receptor‐Mediated Responses by Placental Hofbauer Cells (HBCs): A Potential Pro‐Inflammatory Role for Fetal M2 Macrophages. American Journal Of Reproductive Immunology 2014, 73: 22-35. PMID: 25345551, PMCID: PMC4268350, DOI: 10.1111/aji.12336.Peer-Reviewed Original ResearchConceptsHofbauer cellsHuman umbilical vein endothelial cellsM2 macrophagesTLR-3TLR-4Potential pro-inflammatory roleToll-like receptor functionPlacental villous coreAdverse pregnancy outcomesPro-inflammatory rolePlacental Hofbauer cellsIL-6 secretionToll-like receptorsPolycytidylic acid treatmentUmbilical vein endothelial cellsFetal inflammationPregnancy outcomesVein endothelial cellsTLR agonistsTLR-2Cytokine secretionInflammatory responseVillous corePlacental fibroblastsReceptor functionTrophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages
Aldo PB, Racicot K, Craviero V, Guller S, Romero R, Mor G. Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages. American Journal Of Reproductive Immunology 2014, 72: 270-284. PMID: 24995492, PMCID: PMC4230492, DOI: 10.1111/aji.12288.Peer-Reviewed Original ResearchConceptsUnique macrophage phenotypeMacrophage phenotypeMonocyte differentiationFirst trimester trophoblast cellsPeripheral blood monocytesPhenotype of macrophagesCD14 surface expressionNormal deciduaHealthy pregnancyIL-10Chemokine productionEarly pregnancyIP-10Monocyte exposureIL-1BBlood monocytesDecidual phenotypePhagocytic capacityTrophoblast cellsFlow cytometryMorphologic changesMacrophagesMonocytesSurface expressionPlacenta development
2013
Decreased Levels of Folate Receptor‐β and Reduced Numbers of Fetal Macrophages (Hofbauer Cells) in Placentas from Pregnancies with Severe Pre‐Eclampsia
Tang Z, Buhimschi IA, Buhimschi CS, Tadesse S, Norwitz E, Niven‐Fairchild T, Huang S, Guller S. Decreased Levels of Folate Receptor‐β and Reduced Numbers of Fetal Macrophages (Hofbauer Cells) in Placentas from Pregnancies with Severe Pre‐Eclampsia. American Journal Of Reproductive Immunology 2013, 70: 104-115. PMID: 23480364, PMCID: PMC3686834, DOI: 10.1111/aji.12112.Peer-Reviewed Original ResearchConceptsSpontaneous preterm birthPre-eclampsiaAge-matched normotensive controlsPreterm pre-eclampsiaSevere pre eclampsiaPathophysiology of PEPregnancy complicationsPreterm birthNormotensive controlsHofbauer cellsFetal mortalityUnknown etiologyPlacental expressionSPTB groupMacrophage functionPlacentaSevere preFetal macrophagesWestern blottingMajor causeProtein expressionPlacental genesReverse transcription-PCR analysisMarker mRNAsTranscription-PCR analysis
2012
Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells
Tang Z, Niven-Fairchild T, Tadesse S, Norwitz ER, Buhimschi CS, Buhimschi IA, Guller S. Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells. Endocrinology 2012, 154: 471-482. PMID: 23142809, PMCID: PMC3529384, DOI: 10.1210/en.2012-1575.Peer-Reviewed Original ResearchConceptsHofbauer cellsGC treatmentGC administrationCD163 expressionDEX treatmentCD68 mRNASpontaneous preterm birthMacrophage marker genesPlacental explant culturesAntiinflammatory M2 macrophagesHuman term placentaExpression of FRPreterm deliveryPreterm birthCD163 mRNAM2 macrophagesReceptor mRNAGC actionSynthetic GCFetal capillariesTerm placentaPlacental fibroblastsFlow cytometryCD163 proteinFetal macrophages
2011
Isolation of Hofbauer Cells from Human Term Placentas with High Yield and Purity
Tang Z, Tadesse S, Norwitz E, Mor G, Abrahams VM, Guller S. Isolation of Hofbauer Cells from Human Term Placentas with High Yield and Purity. American Journal Of Reproductive Immunology 2011, 66: 336-348. PMID: 21545365, PMCID: PMC3154981, DOI: 10.1111/j.1600-0897.2011.01006.x.Peer-Reviewed Original ResearchConceptsHofbauer cellsHuman term placentaTerm placentaComplications of pregnancyCell typesMacrophage functionPhagocytotic activityFlow cytometryVillus functionConsensus methodologyVacuolated appearancePhagocytosis assaysPlacentaPercoll gradientCTFuture studiesFibroblastsHBCCellsDaysHigh levelsComplicationsCulture mediumPregnancyMacrophages
2010
Focal Increases of Fetal Macrophages in Placentas from Pregnancies with Histological Chorioamnionitis: Potential Role of Fibroblast Monocyte Chemotactic Protein‐1
Toti P, Arcuri F, Tang Z, Schatz F, Zambrano E, Mor G, Niven‐Fairchild T, Abrahams VM, Krikun G, Lockwood CJ, Guller S. Focal Increases of Fetal Macrophages in Placentas from Pregnancies with Histological Chorioamnionitis: Potential Role of Fibroblast Monocyte Chemotactic Protein‐1. American Journal Of Reproductive Immunology 2010, 65: 470-479. PMID: 21087336, PMCID: PMC3071455, DOI: 10.1111/j.1600-0897.2010.00927.x.Peer-Reviewed Original ResearchConceptsHistopathological chorioamnionitisMCP-1 mRNAFocal increaseMonocyte chemotactic protein-1Subset of pregnanciesAdverse neonatal outcomesProtein expressionPro-inflammatory cytokinesMaternal-fetal interfaceChemotactic protein-1MCP-1 expressionTerm placental villiHistological chorioamnionitisNeonatal outcomesCytokine cascadeIL-1βInflammatory cytokinesQuantitative real-time PCRFetal monocytesReal-time PCRHuman term placental villiPlacental villiControl groupBacterial productsTreatment of fibroblasts