2021
Maternal respiratory SARS-CoV-2 infection in pregnancy is associated with a robust inflammatory response at the maternal-fetal interface
Lu-Culligan A, Chavan AR, Vijayakumar P, Irshaid L, Courchaine EM, Milano KM, Tang Z, Pope SD, Song E, Vogels CBF, Lu-Culligan WJ, Campbell KH, Casanovas-Massana A, Bermejo S, Toothaker JM, Lee HJ, Liu F, Schulz W, Fournier J, Muenker MC, Moore AJ, Team Y, Konnikova L, Neugebauer KM, Ring A, Grubaugh ND, Ko AI, Morotti R, Guller S, Kliman HJ, Iwasaki A, Farhadian SF. Maternal respiratory SARS-CoV-2 infection in pregnancy is associated with a robust inflammatory response at the maternal-fetal interface. Med 2021, 2: 591-610.e10. PMID: 33969332, PMCID: PMC8084634, DOI: 10.1016/j.medj.2021.04.016.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionMaternal-fetal interfaceACE2 expressionNatural killerPregnant womenPlacental cellsAcute respiratory syndrome coronavirus 2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionSARS-CoV-2-infected womenTerm placentaSyndrome coronavirus 2 infectionCoronavirus 2 infectionPotential immune mechanismsRobust inflammatory responseRobust immune responseCoronavirus disease 2019Detectable viral RNAInterferon-related genesLower ACE2 expressionMajority of placentasPregnancy complicationsPlacental histologyHofbauer cellsEarly pregnancyImmune activation
2019
In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines.
Kisanga EP, Tang Z, Guller S, Whirledge S. In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines. Journal Of Visualized Experiments 2019 PMID: 30907868, DOI: 10.3791/58942.Peer-Reviewed Original ResearchConceptsCell movementCell migrationHuman first trimester trophoblast cell lineFirst trimester trophoblast cell lineIntrauterine growth restrictionCell linesTrophoblast cell linePregnancy disordersEarly pregnancyGrowth restrictionMaternal vasculatureTrophoblast migrationMigratory capacityPlacental developmentPotential mechanismsCellular changesVitro assaysInvasionTrophoblastProliferationThorough examinationMigrationAssaysIndividual factorsPregnancy
2014
Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages
Aldo PB, Racicot K, Craviero V, Guller S, Romero R, Mor G. Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages. American Journal Of Reproductive Immunology 2014, 72: 270-284. PMID: 24995492, PMCID: PMC4230492, DOI: 10.1111/aji.12288.Peer-Reviewed Original ResearchConceptsUnique macrophage phenotypeMacrophage phenotypeMonocyte differentiationFirst trimester trophoblast cellsPeripheral blood monocytesPhenotype of macrophagesCD14 surface expressionNormal deciduaHealthy pregnancyIL-10Chemokine productionEarly pregnancyIP-10Monocyte exposureIL-1BBlood monocytesDecidual phenotypePhagocytic capacityTrophoblast cellsFlow cytometryMorphologic changesMacrophagesMonocytesSurface expressionPlacenta development
2007
Role of Hypoxia-Inducible Transcription Factors 1α and 2α in the Regulation of Plasminogen Activator Inhibitor-1 Expression in a Human Trophoblast Cell Line
Meade ES, Ma YY, Guller S. Role of Hypoxia-Inducible Transcription Factors 1α and 2α in the Regulation of Plasminogen Activator Inhibitor-1 Expression in a Human Trophoblast Cell Line. Placenta 2007, 28: 1012-1019. PMID: 17570486, PMCID: PMC2001228, DOI: 10.1016/j.placenta.2007.04.005.Peer-Reviewed Original ResearchConceptsPAI-1 expressionHTR-8/SVneo cellsPlasminogen activator inhibitorTrophoblast cell linePAI-1HIF-1alphaHIF-2alphaFirst trimester extravillous trophoblast cell lineHuman trophoblast cell linePlasmin-dependent fibrinolysisExtravillous trophoblast cell linePlasminogen activator inhibitor-1 expressionHypoxia-inducible transcription factor-1αHypoxic conditionsPAI-1 protein levelsCell linesPAI-1 mRNAEarly pregnancyExtracellular matrix degradationHypoxia-inducible transcription factorsQuantitative real-time PCRReal-time PCRActivator inhibitorFactor-1αWestern blotting
2006
First-trimester trophoblast cell model gene response to hypoxia
Koklanaris N, Nwachukwu JC, Huang SJ, Guller S, Karpisheva K, Garabedian M, Lee MJ. First-trimester trophoblast cell model gene response to hypoxia. American Journal Of Obstetrics And Gynecology 2006, 194: 687-693. PMID: 16522398, DOI: 10.1016/j.ajog.2006.01.067.Peer-Reviewed Original ResearchConceptsReverse transcriptase-polymerase chain reactionQuantitative reverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionPolymerase chain reactionHypoxia-responsive genesHTR-8/SVneoChain reactionCell linesTrophoblast cell linePregnancy outcomesEarly pregnancyMotility/migrationHypoxic environmentTotal RNAHypoxiaPlacentationMicroarray studiesGene responsesPreeclampsiaPregnancy