2024
A complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell–cell contacts
Johnson B, Iuliano M, Lam T, Biederer T, De Camilli P. A complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell–cell contacts. Journal Of Cell Biology 2024, 223: e202311137. PMID: 39158698, PMCID: PMC11334333, DOI: 10.1083/jcb.202311137.Peer-Reviewed Original ResearchConceptsPlasma membraneNon-vesicular lipid transferSites of cell contactC-terminus motifsCell contact-dependent signalsContact-dependent signalingCell-cell contactER/PM junctionsTMEM24ER proteinsPM proteinsSynCAM 1Cell adhesion moleculesCellular functionsLipid transferC2CD2Phospholipid transportLipid transportCell contactProteinAdhesion moleculesCalcium homeostasisCellsFamily membersParalogs
2023
RBG Motif Bridge-Like Lipid Transport Proteins: Structure, Functions, and Open Questions
Hanna M, Guillén-Samander A, De Camilli P. RBG Motif Bridge-Like Lipid Transport Proteins: Structure, Functions, and Open Questions. Annual Review Of Cell And Developmental Biology 2023, 39: 409-434. PMID: 37406299, DOI: 10.1146/annurev-cellbio-120420-014634.Peer-Reviewed Original ResearchLipid transfer proteinMembrane contact sitesVesicle-mediated trafficTransport of lipidsPutative physiological roleEukaryotic cellsEndocytic pathwayContact sitesLipid transportPhysiological roleTransfer proteinProteinHydrophobic channelRod-like structureLipidsEntire lengthDevelopmental disordersCytosolMutationsNew familyTransportPathwayMechanismMembraneCells
2022
ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling
Hancock-Cerutti W, Wu Z, Xu P, Yadavalli N, Leonzino M, Tharkeshwar AK, Ferguson SM, Shadel GS, De Camilli P. ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling. Journal Of Cell Biology 2022, 221: e202106046. PMID: 35657605, PMCID: PMC9170524, DOI: 10.1083/jcb.202106046.Peer-Reviewed Original ResearchConceptsParkinson's diseasePD pathogenesisLeucine-rich repeat kinase 2 (LRRK2) G2019S mutationCGAS-STING pathwayAccumulation of lysosomesDNA-sensing cGAS-STING pathwayImmune activationLipid profileSTING signalingG2019S mutationAutosomal recessive Parkinson's diseaseRecessive Parkinson's diseaseModel human cell linesHuman cell linesCell linesPathogenesisLate endosomes/lysosomesDiseaseVPS13CEndosomes/lysosomesCurrent studyTransfer proteinActivationCellsPathway
2021
Cooperative function of synaptophysin and synapsin in the generation of synaptic vesicle-like clusters in non-neuronal cells
Park D, Wu Y, Lee SE, Kim G, Jeong S, Milovanovic D, De Camilli P, Chang S. Cooperative function of synaptophysin and synapsin in the generation of synaptic vesicle-like clusters in non-neuronal cells. Nature Communications 2021, 12: 263. PMID: 33431828, PMCID: PMC7801664, DOI: 10.1038/s41467-020-20462-z.Peer-Reviewed Original ResearchConceptsNon-neuronal cellsSV clustersSynaptic vesiclesSmall synaptic-like microvesiclesSV membrane proteinsSynaptic-like microvesiclesSV proteinsDiffuse cytosolic distributionMembrane proteinsReconstitution systemCytosolic distributionCooperative functionSuch vesiclesMechanistic insightsLiquid-like propertiesPowerful modelPhysiological formationProteinSynapsinVesiclesCellsSynaptic transmissionAssembly of structuresDefining featureLiquid condensate
2020
Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells
Benedetti L, Marvin JS, Falahati H, Guillén-Samander A, Looger LL, De Camilli P. Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells. ELife 2020, 9: e63230. PMID: 33174843, PMCID: PMC7735757, DOI: 10.7554/elife.63230.Peer-Reviewed Original ResearchConceptsProtein fusion partnersSmall subcellular volumesSubcellular optogeneticsOrganelle contactsHomodimerization interfaceProtein modulesMammalian cellsBiological processesPhysiological processesSubcellular organellesLow temperature preincubationSimultaneous photoactivationFusion partnerCell preincubationWhole cellsSubcellular volumesConcatemerizationSpatial controlSpatiotemporal confinementCellsNeurosporaOrganellesHeterodimersVIVIDProteinDynamin Function in Exocytosis and Endocytosis Coupling of Dense-Core Vesicles in Pancreatic Beta Cells
Fan F, Wendlick J, Tamarina N, Wu Y, Ferguson S, Philipson L, De Camilli P, Lou X. Dynamin Function in Exocytosis and Endocytosis Coupling of Dense-Core Vesicles in Pancreatic Beta Cells. Biophysical Journal 2020, 118: 488a. DOI: 10.1016/j.bpj.2019.11.2700.Peer-Reviewed Original Research
2009
Mechanistic Insights into Membrane Remodeling Through BAR‐Domain Proteins
Frost A, Mim C, Perera R, Spasov K, Egelman E, De Camilli P, Unger V. Mechanistic Insights into Membrane Remodeling Through BAR‐Domain Proteins. The FASEB Journal 2009, 23: 82.2-82.2. DOI: 10.1096/fasebj.23.1_supplement.82.2.Peer-Reviewed Original ResearchF-BAR domainBAR domain proteinsMembrane-bound stateDifferent molecular surfacesMembrane remodelingCellular physiologyMembrane curvatureElectron cryomicroscopyMechanistic insightsCooperative assemblyConstituent membranesProteinStructural informationMolecular surfaceFunctional stateMembraneDomainSubtle changesCryomicroscopyBilayer substratePhysiologyStructural modelAssemblyRemodelingCells
2008
Alpha and Gamma Isoforms of the Type I Phosphatidylinositol 4‐phosphate 5‐kinase Regulate Distinct Stages of the Ca2+ Response in Mast Cells
Vasudevan L, Jeromin A, Volpicelli‐Daley L, De Camilli P, Baird B, Holowka D. Alpha and Gamma Isoforms of the Type I Phosphatidylinositol 4‐phosphate 5‐kinase Regulate Distinct Stages of the Ca2+ Response in Mast Cells. The FASEB Journal 2008, 22: 804.1-804.1. DOI: 10.1096/fasebj.22.1_supplement.804.1.Peer-Reviewed Original ResearchMast cellsER storesMast cell responsesRBL-2H3 mast cellsType IInflammatory mediatorsKO miceBone marrowStore-operated Ca 2IgE receptorCell responsesAntigen crosslinkingExocytotic releaseIP 3 receptorsCa 2Hydrolysis of phosphatidylinositolAntigenReceptorsEndoplasmic reticulumCellular localizationGamma isoformsInfluxCells
2000
A Functional Link between Dynamin and the Actin Cytoskeleton at Podosomes
Ochoa G, Slepnev V, Neff L, Ringstad N, Takei K, Daniell L, Kim W, Cao H, McNiven M, Baron R, De Camilli P. A Functional Link between Dynamin and the Actin Cytoskeleton at Podosomes. Journal Of Cell Biology 2000, 150: 377-390. PMID: 10908579, PMCID: PMC2180219, DOI: 10.1083/jcb.150.2.377.Peer-Reviewed Original ResearchConceptsDynamin 2Rous sarcoma virus resultsFunctional linkAttachment sitesDynamin familyActin cytoskeletonActin turnoverGFP-actinAdhesion structuresPlasma membranePodosomesAdhesion plaquesDynaminCell transformationNontransformed cellsTubular invaginationsFluorescence recoverySimilar turnoverActinSubstratumVirus resultsCellsTurnoverCytoskeletonDramatic changes
1999
AP-2 Recruitment to Synaptotagmin Stimulated by Tyrosine-Based Endocytic Motifs
Haucke V, De Camilli P. AP-2 Recruitment to Synaptotagmin Stimulated by Tyrosine-Based Endocytic Motifs. Science 1999, 285: 1268-1271. PMID: 10455054, DOI: 10.1126/science.285.5431.1268.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Protein Complex alpha SubunitsAdaptor Proteins, Vesicular TransportAnimalsBinding SitesCalcium-Binding ProteinsCattleCell MembraneCHO CellsClathrinCoated Pits, Cell-MembraneCricetinaeEndocytosisMembrane GlycoproteinsMembrane ProteinsNerve Tissue ProteinsNeuronsOligopeptidesPhospholipase DProtein BindingRatsRecombinant Fusion ProteinsSynaptic MembranesSynaptotagminsTyrosineConceptsAP-2 recruitmentEndocytic motifAP-2Cargo proteinsPlasma membraneTyrosine-based endocytic motifClathrin adaptor protein AP-2Adaptor protein AP-2Nucleation of clathrinNon-neuronal cellsProtein synaptotagminDocking siteSynaptotagminClathrinMotifProteinRecruitmentMembraneEndocytosisTyrosineBindingCellsPeptides
1997
Identification and characterization of homologues of the Exocyst component Sec10p
Guo W, Roth D, Gatti E, De Camilli P, Novick P. Identification and characterization of homologues of the Exocyst component Sec10p. FEBS Letters 1997, 404: 135-139. PMID: 9119050, DOI: 10.1016/s0014-5793(97)00109-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBrainCloning, MolecularCOS CellsExocytosisFungal ProteinsGolgi ApparatusHumansMammalsMolecular Sequence DataPolymerase Chain ReactionRecombinant ProteinsRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidTranscription, GeneticTransfectionVesicular Transport ProteinsConceptsC. elegans proteinsCharacterization of homologuesAmino acid identityBroad tissue distributionGolgi trafficMammalian counterpartsYeast SaccharomycesAcid identityGene productsCOS cellsWestern blot analysisSec10pPeripheral cytoplasmExocystBlot analysisProteinTissue distributionImmunofluorescence stainingSec8pCellsSaccharomycesCloningHomologuesExocytosisCytoplasm
1994
Formation of synaptic vesicles
Mundigl O, De Camilli P. Formation of synaptic vesicles. Current Opinion In Cell Biology 1994, 6: 561-567. PMID: 7986534, DOI: 10.1016/0955-0674(94)90077-9.Peer-Reviewed Original ResearchConceptsSynaptic vesiclesNew synaptic vesiclesSV membranesTransport vesiclesEvolutionary originNon-peptide neurotransmittersMembrane proteinsSecretory organellesFocal signalingVesiclesExocytosisMolecular compositionCellsGamma-aminobutyric acidOrganellesSignalingNerve cellsProteinDepolarization-induced Ca2PathwaySignificant progressNeurotransmittersMembraneAssemblyNerve terminals
1990
Synapsins in the vertebrate retina: Absence from ribbon synapses and heterogeneous distribution among conventional synapses
Mandell J, Townes-Anderson E, Czernik A, Cameron R, Greengard P, De Camilli P. Synapsins in the vertebrate retina: Absence from ribbon synapses and heterogeneous distribution among conventional synapses. Neuron 1990, 5: 19-33. PMID: 2114884, DOI: 10.1016/0896-6273(90)90030-j.Peer-Reviewed Original ResearchConceptsConventional synapsesRibbon synapsesAmacrine cellsBipolar cellsIntrinsic membrane proteinsSynapsin IAdult rat retinaNerve terminal phosphoproteinVertebrate retinaMembrane proteinsNerve terminalsRat retinaVesicle clusteringSalamander retinaSynapse typesSynaptic vesiclesMolecular differencesRetinaRod cellsSynapsesCone cellsSynapsinCellsSynaptophysinPhosphoprotein
1988
Cell-specific localization of the α-subunit of calcium/calmodulin-dependent protein kinase II in Purkinje cells in rodent cerebellum
Walaas S, Lai Y, Gorelick F, DeCamilli P, Moretti M, Greengard P. Cell-specific localization of the α-subunit of calcium/calmodulin-dependent protein kinase II in Purkinje cells in rodent cerebellum. Brain Research 1988, 4: 233-242. DOI: 10.1016/0169-328x(88)90029-0.Peer-Reviewed Original ResearchCalmodulin-dependent protein kinase type IICalcium/calmodulin-dependent protein kinase type IIProtein kinase type IIKinase type IIΑ-subunitΒ-subunitCalmodulin-dependent protein kinase IICalcium/calmodulin-dependent protein kinase IIDistinct isozymic formsProtein kinase IINormal rat cerebellumNon-Purkinje cellsKinase IICell-specific localizationRestricted localizationRodent cerebellumIsozymic formsPurkinje cellsDifferent cellsImmunocytochemical analysisMutant miceSubunitsCellsCerebellar mutantsType II
1984
Immunocytochemistry as a tool in the study of neurotransmitter actions
De Camilli P, Navone F. Immunocytochemistry as a tool in the study of neurotransmitter actions. Trends In Pharmacological Sciences 1984, 5: 300-303. DOI: 10.1016/0165-6147(84)90451-6.Peer-Reviewed Original ResearchSpecific cellular functionsProtein phosphorylation reactionsVariety of phosphoproteinsCellular functionsProtein phosphorylationImportant general mechanismProtein kinaseSecond messengerPhysiological functionsPhosphorylation reactionGeneral mechanismBiochemical informationMammalian brainNeurotransmitter actionPhosphoproteinKinasePhosphorylationImmunocytochemistryNerve cellsTarget neuronsMessengerCellsFunction