Ligia Simoes Braga Boisserand, PhD
Associate Research ScientistCards
About
Research
Publications
2025
Multicenter SPAN Trial of Fasudil in Ischemic Stroke.
Imai T, Morais A, Jin X, Qin T, Lamb J, Nagarkatti K, Chen M, Boisserand L, Patel R, Kumskova M, Chauhan A, Dhandapani K, Khan M, Kamat P, Shi Y, Cao S, Sanganahalli B, Mandeville J, Lyden P, Hess D, Leira E, Chauhan A, Aronowski J, McCullough L, Koehler R, Sansing L, Diniz M, Ayata C. Multicenter SPAN Trial of Fasudil in Ischemic Stroke. Stroke 2025 PMID: 40421536, DOI: 10.1161/strokeaha.125.050977.Peer-Reviewed Original ResearchSpontaneously hypertensive ratsRho-associated kinase inhibitorHypertensive ratsAged miceActive treatment armsIschemic strokeModified intention-to-treat cohortKinase inhibitorsPrimary outcome end pointsIntention-to-treat cohortTest performanceEfficacy of fasudilPer-protocol populationOutcome end pointsMiddle cerebral artery occlusion modelHealthy young miceDiet-induced obese miceRodent modelsTrials NetworkTherapeutic profileFocal ischemic strokeTreatment armsYoung miceVehicle armSecondary outcomesDeterminants of Outcome After Endovascular Middle Cerebral Artery Occlusion in Rats in the SPAN Trial.
Jin X, Morais A, Imai T, Lamb J, Nagarkatti K, Boisserand L, Beatty H, Sansing L, Khan M, Dhandapani K, Kamat P, Hess D, Patel R, Kumskova M, Chauhan A, McCullough L, Aronowski J, Leira E, Shi Y, Avery B, Koehler R, Lyden P, Ayata C. Determinants of Outcome After Endovascular Middle Cerebral Artery Occlusion in Rats in the SPAN Trial. Stroke 2025 PMID: 40396268, DOI: 10.1161/strokeaha.125.051235.Peer-Reviewed Original ResearchMiddle cerebral artery occlusionSpontaneously hypertensive ratsCerebral artery occlusionProcedural predictorsNeurological outcomeHypertensive ratsArtery occlusionTransient middle cerebral artery occlusionActive treatment armsEndovascular middle cerebral artery occlusionSprague-Dawley ratsDeterminants of outcomeDependent outcome variablesOcclusal outcomesIndependent predictorsTreatment armsHippocampal infarctionInfarct volumeMultivariate analysisPreclinical trialsAnesthesia exposureImpact stroke outcomesMulticenter approachProcedural variablesRatsUric Acid Stroke Cerebroprotection Transcended Sex, Age, and Comorbidities in a Multicenter Preclinical Trial
Patel R, Kumskova M, Kodali H, Budnik I, Kuznetsov V, Jain A, Jha A, Thedens D, Dhanesha N, Sutariya B, Nagarkatti K, Lamb J, Kamat P, Shi Y, Avery B, Imai T, Jin X, Chauhan A, Boisserand L, Khan M, Dhandapani K, Sanganahalli B, Sansing L, Hess D, Koehler R, McCullough L, Aronowski J, Ayata C, Diniz M, Lyden P, Planas A, Chamorro A, Chauhan A, Leira E, Investigators O. Uric Acid Stroke Cerebroprotection Transcended Sex, Age, and Comorbidities in a Multicenter Preclinical Trial. Stroke 2025, 56: 965-973. PMID: 40091742, PMCID: PMC11932773, DOI: 10.1161/strokeaha.124.048748.Peer-Reviewed Original ResearchConceptsUA-treated animalsPrimary functional outcomeFunctional outcomesMiddle cerebral artery filament occlusionModified intention-to-treat populationIntention-to-treat populationUric acidHuman clinical trialsImprove functional outcomesStudy drugIntravenous salineEffects of UARelevant to patientsClinical trialsPrimary outcomeSaline controlsSecondary outcomesPreclinical trialsDay 2Survival rateDay 7Filament occlusionGroups of animalsComorbiditiesDiverse comorbidities
2024
Incidence de la circulation lymphatique méningée sur la réponse à un accident vasculaire cérébral
Thomas J, Boisserand L, Kamouh M. Incidence de la circulation lymphatique méningée sur la réponse à un accident vasculaire cérébral. Médecine/sciences 2024, 40: 604-608. PMID: 39303108, PMCID: PMC11742258, DOI: 10.1051/medsci/2024086.Peer-Reviewed Original ResearchBiological and Procedural Predictors of Outcome in the Stroke Preclinical Assessment Network (SPAN) Trial
Morais A, Imai T, Jin X, Locascio J, Boisserand L, Herman A, Chauhan A, Lamb J, Nagarkatti K, Diniz M, Kumskova M, Dhanesha N, Kamat P, Khan M, Dhandapani K, Patel R, Sutariya B, Shi Y, van Leyen K, Kimberly W, Hess D, Aronowski J, Leira E, Koehler R, Chauhan A, Sansing L, Lyden P, Ayata C. Biological and Procedural Predictors of Outcome in the Stroke Preclinical Assessment Network (SPAN) Trial. Circulation Research 2024, 135: 575-592. PMID: 39034919, PMCID: PMC11428171, DOI: 10.1161/circresaha.123.324139.Peer-Reviewed Original ResearchConceptsMiddle cerebral artery occlusionFilament middle cerebral artery occlusionHigh-fat dietProcedural variablesHigh-fatOlder ageStroke outcomeBlood flow monitoringCerebral blood flow monitoringPredictors of outcomeBody weightProspective designInfluence stroke outcomeAssociated with smaller infarctsCerebral artery occlusionExperimental focal cerebral ischemiaFocal cerebral ischemiaLonger anesthesia durationExperimental stroke modelsBlood flow dropAcute stroke interventionExperimental stroke outcomesMulticenter prospective designProcedural predictorsPreclinical studiesVEGF-C prophylaxis favors lymphatic drainage and modulates neuroinflammation in a stroke model
Boisserand L, Geraldo L, Bouchart J, Kamouh M, Lee S, Sanganahalli B, Spajer M, Zhang S, Lee S, Parent M, Xue Y, Skarica M, Yin X, Guegan J, Boyé K, Leser F, Jacob L, Poulet M, Li M, Liu X, Velazquez S, Singhabahu R, Robinson M, Askenase M, Osherov A, Sestan N, Zhou J, Alitalo K, Song E, Eichmann A, Sansing L, Benveniste H, Hyder F, Thomas J. VEGF-C prophylaxis favors lymphatic drainage and modulates neuroinflammation in a stroke model. Journal Of Experimental Medicine 2024, 221: e20221983. PMID: 38442272, PMCID: PMC10913814, DOI: 10.1084/jem.20221983.Peer-Reviewed Original ResearchConceptsVascular endothelial growth factor-CDeep cervical lymph nodesCentral nervous systemEffect of vascular endothelial growth factor-CMeningeal lymphatic vesselsAmeliorated motor performanceCervical lymph nodesIschemic strokeVEGF-C overexpressionIncreased BDNF signalingAcute ischemic strokeBrain cellsIncreased CSF drainageIschemic stroke outcomesModel of ischemic strokeMouse model of ischemic strokeImmune surveillanceCSF drainageLymph nodesFluid drainageNucleus RNA sequencingLymphatic growthLymphatic drainageMouse modelBDNF signaling
2023
Embracing Heterogeneity in The Multicenter Stroke Preclinical Assessment Network (SPAN) Trial
Morais A, Locascio J, Sansing L, Lamb J, Nagarkatti K, Imai T, van Leyen K, Aronowski J, Koenig J, Bosetti F, Lyden P, Ayata C, Bosetti F, Koenig J, Lyden P, Lamb J, Nagarkatti K, Hess D, Kamat P, Khan M, Dhandapani K, Arbab A, Siddiqui S, Smith C, Nisar M, Leira E, Chauhan A, Dhanesha N, Patel R, Kumskova M, Thedens D, Wang K, Ayata C, Morais A, Imai T, Qin T, Jin X, Erdogan T, Yu L, Mandeville J, Kimberly W, Whittier J, Lo E, Arai K, Van Leyen K, Sansing L, Hyder F, Mihailovic J, Sanganahalli B, Diaz-Perez S, Velazquez S, Beatty H, Johnson C, Herman A, Boisserand L, Immakavar E, Koehler R, Dawson T, Dawson V, Shi Y, Avery B, Lannon S, Bibic A, Akhter K, Karuppagounder S, Aronowski J, McCullough L, Obertas L, Goh A, Huang S, Chauhan A. Embracing Heterogeneity in The Multicenter Stroke Preclinical Assessment Network (SPAN) Trial. Stroke 2023, 54: 620-631. PMID: 36601951, PMCID: PMC9870939, DOI: 10.1161/strokeaha.122.040638.Peer-Reviewed Original ResearchConceptsTotal anesthesia durationAnesthesia durationIndependent predictorsMultivariable analysisClinical trialsTransient focal cerebral ischemiaFocal cerebral ischemiaCerebral blood flowLaser Doppler flowCentralized randomizationModified intentionTreat populationExperimental strokeCerebral ischemiaDoppler flowRodent modelsMCAOBlood flowNetwork trialFlow dropPreclinical trialsMouse cohortsStudy designTrialsTrial onset
2022
The Stroke Preclinical Assessment Network: Rationale, Design, Feasibility, and Stage 1 Results
Lyden PD, Bosetti F, Diniz MA, Rogatko A, Koenig JI, Lamb J, Nagarkatti KA, Cabeen RP, Hess DC, Kamat PK, Khan MB, Wood K, Dhandapani K, Arbab AS, Leira EC, Chauhan AK, Dhanesha N, Patel RB, Kumskova M, Thedens D, Morais A, Imai T, Qin T, Ayata C, Boisserand LSB, Herman AL, Beatty HE, Velazquez SE, Diaz-Perez S, Sanganahalli BG, Mihailovic JM, Hyder F, Sansing LH, Koehler RC, Lannon S, Shi Y, Karuppagounder SS, Bibic A, Akhter K, Aronowski J, McCullough LD, Chauhan A, Goh A, Siddiqui S, Sheth K, Matouk C, Cruz C, Zhou J, Dawson V, Dawson T, Liang J, van Zijl P, Zeiler S, Taylor Kimberly W, Erdogan T, Yu L, Mandeville J, Whittier J. The Stroke Preclinical Assessment Network: Rationale, Design, Feasibility, and Stage 1 Results. Stroke 2022, 53: 1802-1812. PMID: 35354299, PMCID: PMC9038686, DOI: 10.1161/strokeaha.121.038047.Peer-Reviewed Original ResearchConceptsPreclinical assessmentStroke treatmentCandidate treatmentMiddle cerebral artery occlusion (MCAO) surgeryClinical stroke trialsSuccessful stroke treatmentInclusion/exclusion criteriaStroke clinical trialsClinical trial designYoung male animalsComorbid diseasesOcclusion surgeryCerebral ischemiaNeurological disabilityComorbid conditionsStroke trialsProtocol adherenceBlinded assessmentSuch therapyClinical trialsClinical studiesExclusion criteriaTrial designAged animalsOutcome assessment
2021
Publisher Correction: VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours
Song E, Mao T, Dong H, Boisserand LSB, Antila S, Bosenberg M, Alitalo K, Thomas JL, Iwasaki A. Publisher Correction: VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours. Nature 2021, 590: e34-e34. PMID: 33500588, DOI: 10.1038/s41586-021-03204-z.Peer-Reviewed Original Research
2020
Les vaisseaux lymphatiques méningés, une cible potentielle pour le traitement des tumeurs cérébrales
Thomas JL, Song E, Boisserand L, Iwasaki A. Les vaisseaux lymphatiques méningés, une cible potentielle pour le traitement des tumeurs cérébrales. Médecine/sciences 2020, 36: 709-713. PMID: 32821046, PMCID: PMC8158397, DOI: 10.1051/medsci/2020141.Peer-Reviewed Original Research